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The map label for this gene is ghrA [H]

Identifier: 146340389

GI number: 146340389

Start: 3610499

End: 3611449

Strand: Direct

Name: ghrA [H]

Synonym: BRADO3422

Alternate gene names: 146340389

Gene position: 3610499-3611449 (Clockwise)

Preceding gene: 146340388

Following gene: 146340393

Centisome position: 48.42

GC content: 68.14

Gene sequence:

>951_bases
ATGATCCAAGGTCAGATGCAAGGCGTGATCAGCGTTGCGCTCCTCAGCCGTCCCGGGCTGATGGATTCGATGAAGGCGCT
GCTCGCGCGCGAACTGCCTGGCGTGACCGTTTCGTCCTGGCCGGAGGCCGCCGCGCGTGACGCGGAGCTTGCGGTGTGCT
GGAAGCCGGAGCCCGGCGCGCTGGCGGCGATGCCGAAGCTGAAGCTCATTCATGCGATCGCAGCCGGCGTCGACAACATC
CTGTCTGATCCAACCGTGCCCGATGTGCCGCTGTGCCGGATCGTCGACCCCGGCATCACCGCGGCCATGACCGAGTTCGT
GCTGTGGGGCGCGCTGTATTTCCACCGCGATTTCGACCGGGTCATCAGCAATGCGCGTGAGGGCCGCTGGCAGCGCTATG
ACCAGCGCGCGGCAAAGGACGTGAGGGTCGGCATTCTCGGACTTGGCGAGCTCGGCACCGACGCCGCGCGCCGTCTCGCG
GAGCTTGGCTTCACGGTGAGCGCCTGGTCGCGCTCGCCGAAGCAGTTCGCTGGCGTAAGGACGTTCAGCGGCGCAGCTGC
GCTCGACGCATTCCTGAGCGAGACCGATATTCTTGTCTCGCTGCTGCCGCTGACGCCCTCGACCTTGGGGCTTCTGAATA
CGGCCCGGCTGAGCCGCCTGCCCAAGGGCGCCGCGCTGATCCTGTGCAGCCGCGGCGAGCACGTCGTGATGGATGATCTG
GTCGCACTGCTTCGCAGCGGCCATTTGCGCGGCGCCGTGCTCGACGTGTTCGAGCGGGAGCCGCTGCCAGCGGAGCATCC
GCTGTGGCGTGAGCCCGGCGTGCTCGTGACGCCGCACATGGCGGCGATCGCATCCTGGGAGGCCATCACGCTGCAGGTCG
CCGAGAACGCGCGGCGGCTGCTGAGCGGCCAGCCGCTGTTGAACGTGGTCGACCGCGCCCGCGGCTACTGA

Upstream 100 bases:

>100_bases
GGCCGCACGCGTTCGATGAGCGCGTCGATCTCGACAGCGTGCGCAAGACAACGCTGTCGATCGCGATGTTCGTCGCGCAA
TGGTGCGGGCTGCGCAAGGC

Downstream 100 bases:

>100_bases
GAGCCGAGCGCGGTCTCACTCGAGCGTCTGGACCGGAGGAGCGACCGGGCCCCGCGGTGTCGGCCGAGCCGCGGTGGCGG
TCCTGGGCTGGGAAGGCGCA

Product: putative 2-hydroxyacid dehydrogenase family protein

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MIQGQMQGVISVALLSRPGLMDSMKALLARELPGVTVSSWPEAAARDAELAVCWKPEPGALAAMPKLKLIHAIAAGVDNI
LSDPTVPDVPLCRIVDPGITAAMTEFVLWGALYFHRDFDRVISNAREGRWQRYDQRAAKDVRVGILGLGELGTDAARRLA
ELGFTVSAWSRSPKQFAGVRTFSGAAALDAFLSETDILVSLLPLTPSTLGLLNTARLSRLPKGAALILCSRGEHVVMDDL
VALLRSGHLRGAVLDVFEREPLPAEHPLWREPGVLVTPHMAAIASWEAITLQVAENARRLLSGQPLLNVVDRARGY

Sequences:

>Translated_316_residues
MIQGQMQGVISVALLSRPGLMDSMKALLARELPGVTVSSWPEAAARDAELAVCWKPEPGALAAMPKLKLIHAIAAGVDNI
LSDPTVPDVPLCRIVDPGITAAMTEFVLWGALYFHRDFDRVISNAREGRWQRYDQRAAKDVRVGILGLGELGTDAARRLA
ELGFTVSAWSRSPKQFAGVRTFSGAAALDAFLSETDILVSLLPLTPSTLGLLNTARLSRLPKGAALILCSRGEHVVMDDL
VALLRSGHLRGAVLDVFEREPLPAEHPLWREPGVLVTPHMAAIASWEAITLQVAENARRLLSGQPLLNVVDRARGY
>Mature_316_residues
MIQGQMQGVISVALLSRPGLMDSMKALLARELPGVTVSSWPEAAARDAELAVCWKPEPGALAAMPKLKLIHAIAAGVDNI
LSDPTVPDVPLCRIVDPGITAAMTEFVLWGALYFHRDFDRVISNAREGRWQRYDQRAAKDVRVGILGLGELGTDAARRLA
ELGFTVSAWSRSPKQFAGVRTFSGAAALDAFLSETDILVSLLPLTPSTLGLLNTARLSRLPKGAALILCSRGEHVVMDDL
VALLRSGHLRGAVLDVFEREPLPAEHPLWREPGVLVTPHMAAIASWEAITLQVAENARRLLSGQPLLNVVDRARGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=262, Percent_Identity=27.4809160305344, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI23308577, Length=263, Percent_Identity=26.9961977186312, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI61743967, Length=272, Percent_Identity=27.9411764705882, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI4557497, Length=272, Percent_Identity=27.9411764705882, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87081824, Length=295, Percent_Identity=40.3389830508475, Blast_Score=210, Evalue=9e-56,
Organism=Escherichia coli, GI87082289, Length=229, Percent_Identity=27.9475982532751, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789279, Length=203, Percent_Identity=30.0492610837438, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=227, Percent_Identity=28.6343612334802, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25147481, Length=192, Percent_Identity=26.5625, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6324055, Length=225, Percent_Identity=29.3333333333333, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28574286, Length=264, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24585514, Length=260, Percent_Identity=25.7692307692308, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28574282, Length=260, Percent_Identity=25.7692307692308, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28574284, Length=260, Percent_Identity=25.7692307692308, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45552429, Length=260, Percent_Identity=25.7692307692308, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45551003, Length=260, Percent_Identity=25.7692307692308, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28571528, Length=347, Percent_Identity=27.9538904899135, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19921140, Length=248, Percent_Identity=27.8225806451613, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34153; Mature: 34153

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQGQMQGVISVALLSRPGLMDSMKALLARELPGVTVSSWPEAAARDAELAVCWKPEPGA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEECCCCCC
LAAMPKLKLIHAIAAGVDNILSDPTVPDVPLCRIVDPGITAAMTEFVLWGALYFHRDFDR
EECCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
VISNAREGRWQRYDQRAAKDVRVGILGLGELGTDAARRLAELGFTVSAWSRSPKQFAGVR
HHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCHHHHCCCHHHHHCCC
TFSGAAALDAFLSETDILVSLLPLTPSTLGLLNTARLSRLPKGAALILCSRGEHVVMDDL
CCCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHH
VALLRSGHLRGAVLDVFEREPLPAEHPLWREPGVLVTPHMAAIASWEAITLQVAENARRL
HHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHHCCHHEEEHHHHHHHHH
LSGQPLLNVVDRARGY
HCCCHHHHHHHHHCCC
>Mature Secondary Structure
MIQGQMQGVISVALLSRPGLMDSMKALLARELPGVTVSSWPEAAARDAELAVCWKPEPGA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEECCCCCC
LAAMPKLKLIHAIAAGVDNILSDPTVPDVPLCRIVDPGITAAMTEFVLWGALYFHRDFDR
EECCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
VISNAREGRWQRYDQRAAKDVRVGILGLGELGTDAARRLAELGFTVSAWSRSPKQFAGVR
HHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCHHHHCCCHHHHHCCC
TFSGAAALDAFLSETDILVSLLPLTPSTLGLLNTARLSRLPKGAALILCSRGEHVVMDDL
CCCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHH
VALLRSGHLRGAVLDVFEREPLPAEHPLWREPGVLVTPHMAAIASWEAITLQVAENARRL
HHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHHCCHHEEEHHHHHHHHH
LSGQPLLNVVDRARGY
HCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA