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The map label for this gene is aspS [H]

Identifier: 146340332

GI number: 146340332

Start: 3551062

End: 3552834

Strand: Direct

Name: aspS [H]

Synonym: BRADO3359

Alternate gene names: 146340332

Gene position: 3551062-3552834 (Clockwise)

Preceding gene: 146340328

Following gene: 146340333

Centisome position: 47.62

GC content: 66.1

Gene sequence:

>1773_bases
ATGCATCGCTACCGGACCCATACCTGCGGCGCGCTCCGCGACAGCAACATCGGAGAGACGGTCCGGCTGTCCGGCTGGTG
CCATCGCATCCGCGACCATGGCGGCGTGCTGTTCGTCGACCTGCGCGACCATTACGGCATCACCCAGTGCGTCGTCGACC
CTGACTCCAAAGCGTTCGGGCTGGCCGAAAAGCTGCGCTCGGAGTGGGTCGTGCGCATGGAGGGCAAGGTCCGCCACCGT
CCCGAGGGCACCGAGAACCCGGAGCTGCCGACCGGGCAGGTCGAGCTCTACGTCGCCGACATCGAGGTGCTGGGCCCCGC
GGCCGAGCTGCCGCTGCCGGTGTTCGGCGAGCAGGACTACCCCGAGGACATCCGGCTGAAGTACCGCTTCCTCGACCTGC
GCCGTGACAGGCTGCACCAGAACATCATGACCCGCGGCGCGATCATCGATTCGATGCGCCGGCGGATGAAGGAGCAGGGC
TTCTTCGAATTCCAGACGCCGATCCTGACTGCGTCGTCGCCGGAGGGCGCGCGCGACTTCCTGGTGCCGTCGCGCATCCA
TCCCGGCAAGTTCTACGCGCTGCCGCAGGCGCCGCAGCAGTACAAGCAGCTGCTCATGATGAGCGGCTTCGACCGCTACT
TCCAGATCGCGCCGTGCTTCCGCGACGAGGATCCGCGTGCCGACCGTCTGCCCGGTGAGTTCTATCAGCTCGACGTCGAG
ATGAGCTTCGTCGAGCAGGAGGACGTGTTCGCGGCGATGGAGCCTGTGATCACCGGCGTGTTCGAAGATTTTGCCAAGGG
TAAGCCGGTCACCAAGGGCTGGCCGCGCATTCCGTTCGCGGAAGCGCTGCGCAAATACGGCACCGACAAGCCCGACCTGC
GCAATCCGTTGGAGATGCAGGACGTCTCCGAGCACTTCCGCGGCTCCGGCTTCAAGGTGTTCGCGCGCATGCTCGAGGAC
CCGAAGAACCAGGTCTGGGCGATCCCGGCACCGGGTGGCGGCAGCCGCGCGTTCTGCGACCGCATGAACTCGTGGGCGCA
GGGCGAGGGCCAGCCTGGCCTCGGGTACATCATGTGGCGCGAGGGCGGCGAGGGCGCTGGCCCGCTTGCGAACAACATCG
GCCCGGAGCGGACCGCCGCGATCCGCAGCCAGCTCGGCACCAAGGAGGGCGATGCCGCCTTCTTCGTCGCCGGCGATCCC
GACAAGTTCTGGAAGTTCGCAGGCCTCGCCCGCACCAAGGTCGGCGAGGAGCTGAACCTGATCGACAAGGACCGCTTCGC
GCTGGCCTGGATCGTCGACTTCCCGATGTACGAGTACAACGAGGACGACAAGAAGGTCGACTTCTCGCACAACCCGTTCT
CGATGCCGCAGGGTGGCCTCGAAGCGCTCACCACCAAGGATCCGCTGACCATCAAGGCGTTCCAATACGACATCGCCTGC
AACGGCTACGAGATCGCCTCCGGCGGCATCCGCAACCACAAGCCGGAAGCGATGGTGAAGGCGTTCGAGATCGCGGGCTA
TGGCGAGCAGGAGGTGGTCGACCGCTTCGGCGGCATGTACCGCGCCTTCCAGTACGGCGCCCCGCCGCATGGCGGCATGG
CCGCCGGCGTCGACCGCATCGTGATGCTGCTGTGTGGCACCACCAATCTGCGCGAGATCTCGCTGTTCCCGATGAACCAG
CAGGCGATGGATCTGCTGATGGGCGCGCCATCGGAAGCCACGACGAAGCAGCTGCGCGAGCTGCACATCAAGCCCAACCT
GCCGAACAAGTAG

Upstream 100 bases:

>100_bases
GCGCCGGGGCGGCTCGGTTAAACCCTGCCCGCATTCGGCAACGCGCCCCAGAGGCTTGCGCCGATTCCCTTTTCCTTAAC
GCCGACCATTCAGGACGACC

Downstream 100 bases:

>100_bases
TTCAGTGGAGGCGTGTAGCCCGGATGAACGACAGCGACATCCGGGTTCGCACGCGCAGCTCATGAGACTTCGGATGTCGC
TTTCGCTTCTGACGTAGGGT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MHRYRTHTCGALRDSNIGETVRLSGWCHRIRDHGGVLFVDLRDHYGITQCVVDPDSKAFGLAEKLRSEWVVRMEGKVRHR
PEGTENPELPTGQVELYVADIEVLGPAAELPLPVFGEQDYPEDIRLKYRFLDLRRDRLHQNIMTRGAIIDSMRRRMKEQG
FFEFQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFVEQEDVFAAMEPVITGVFEDFAKGKPVTKGWPRIPFAEALRKYGTDKPDLRNPLEMQDVSEHFRGSGFKVFARMLED
PKNQVWAIPAPGGGSRAFCDRMNSWAQGEGQPGLGYIMWREGGEGAGPLANNIGPERTAAIRSQLGTKEGDAAFFVAGDP
DKFWKFAGLARTKVGEELNLIDKDRFALAWIVDFPMYEYNEDDKKVDFSHNPFSMPQGGLEALTTKDPLTIKAFQYDIAC
NGYEIASGGIRNHKPEAMVKAFEIAGYGEQEVVDRFGGMYRAFQYGAPPHGGMAAGVDRIVMLLCGTTNLREISLFPMNQ
QAMDLLMGAPSEATTKQLRELHIKPNLPNK

Sequences:

>Translated_590_residues
MHRYRTHTCGALRDSNIGETVRLSGWCHRIRDHGGVLFVDLRDHYGITQCVVDPDSKAFGLAEKLRSEWVVRMEGKVRHR
PEGTENPELPTGQVELYVADIEVLGPAAELPLPVFGEQDYPEDIRLKYRFLDLRRDRLHQNIMTRGAIIDSMRRRMKEQG
FFEFQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFVEQEDVFAAMEPVITGVFEDFAKGKPVTKGWPRIPFAEALRKYGTDKPDLRNPLEMQDVSEHFRGSGFKVFARMLED
PKNQVWAIPAPGGGSRAFCDRMNSWAQGEGQPGLGYIMWREGGEGAGPLANNIGPERTAAIRSQLGTKEGDAAFFVAGDP
DKFWKFAGLARTKVGEELNLIDKDRFALAWIVDFPMYEYNEDDKKVDFSHNPFSMPQGGLEALTTKDPLTIKAFQYDIAC
NGYEIASGGIRNHKPEAMVKAFEIAGYGEQEVVDRFGGMYRAFQYGAPPHGGMAAGVDRIVMLLCGTTNLREISLFPMNQ
QAMDLLMGAPSEATTKQLRELHIKPNLPNK
>Mature_590_residues
MHRYRTHTCGALRDSNIGETVRLSGWCHRIRDHGGVLFVDLRDHYGITQCVVDPDSKAFGLAEKLRSEWVVRMEGKVRHR
PEGTENPELPTGQVELYVADIEVLGPAAELPLPVFGEQDYPEDIRLKYRFLDLRRDRLHQNIMTRGAIIDSMRRRMKEQG
FFEFQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFVEQEDVFAAMEPVITGVFEDFAKGKPVTKGWPRIPFAEALRKYGTDKPDLRNPLEMQDVSEHFRGSGFKVFARMLED
PKNQVWAIPAPGGGSRAFCDRMNSWAQGEGQPGLGYIMWREGGEGAGPLANNIGPERTAAIRSQLGTKEGDAAFFVAGDP
DKFWKFAGLARTKVGEELNLIDKDRFALAWIVDFPMYEYNEDDKKVDFSHNPFSMPQGGLEALTTKDPLTIKAFQYDIAC
NGYEIASGGIRNHKPEAMVKAFEIAGYGEQEVVDRFGGMYRAFQYGAPPHGGMAAGVDRIVMLLCGTTNLREISLFPMNQ
QAMDLLMGAPSEATTKQLRELHIKPNLPNK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=598, Percent_Identity=34.4481605351171, Blast_Score=353, Evalue=3e-97,
Organism=Homo sapiens, GI45439306, Length=294, Percent_Identity=28.9115646258503, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI226491502, Length=247, Percent_Identity=26.3157894736842, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI4758762, Length=251, Percent_Identity=24.3027888446215, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI194272210, Length=263, Percent_Identity=25.8555133079848, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI5031815, Length=263, Percent_Identity=25.8555133079848, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788173, Length=588, Percent_Identity=42.1768707482993, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1790571, Length=294, Percent_Identity=26.530612244898, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1789256, Length=294, Percent_Identity=26.530612244898, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI32566633, Length=606, Percent_Identity=32.8382838283828, Blast_Score=288, Evalue=7e-78,
Organism=Caenorhabditis elegans, GI71984122, Length=308, Percent_Identity=25, Blast_Score=79, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17551876, Length=297, Percent_Identity=26.9360269360269, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=657, Percent_Identity=28.158295281583, Blast_Score=221, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6321807, Length=277, Percent_Identity=29.9638989169675, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323011, Length=268, Percent_Identity=25.3731343283582, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24584738, Length=604, Percent_Identity=31.953642384106, Blast_Score=290, Evalue=3e-78,
Organism=Drosophila melanogaster, GI17136276, Length=265, Percent_Identity=27.9245283018868, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921528, Length=243, Percent_Identity=25.9259259259259, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66537; Mature: 66537

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRYRTHTCGALRDSNIGETVRLSGWCHRIRDHGGVLFVDLRDHYGITQCVVDPDSKAFG
CCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCHHH
LAEKLRSEWVVRMEGKVRHRPEGTENPELPTGQVELYVADIEVLGPAAELPLPVFGEQDY
HHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCHHHCCCCCCCCCCC
PEDIRLKYRFLDLRRDRLHQNIMTRGAIIDSMRRRMKEQGFFEFQTPILTASSPEGARDF
CHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEECCCCCCCHHC
LVPSRIHPGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVEQEDVFAAMEPVITGVFEDFAKGKPVTKGWPRIPFAEALRKYGTDKPDLRNPLEMQ
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHH
DVSEHFRGSGFKVFARMLEDPKNQVWAIPAPGGGSRAFCDRMNSWAQGEGQPGLGYIMWR
HHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCHHCCCCCCCCCEEEEEE
EGGEGAGPLANNIGPERTAAIRSQLGTKEGDAAFFVAGDPDKFWKFAGLARTKVGEELNL
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCC
IDKDRFALAWIVDFPMYEYNEDDKKVDFSHNPFSMPQGGLEALTTKDPLTIKAFQYDIAC
CCCCCEEEEEEEECCCCCCCCCCCEECCCCCCCCCCCCCHHHHCCCCCEEEEEEEEEEEE
NGYEIASGGIRNHKPEAMVKAFEIAGYGEQEVVDRFGGMYRAFQYGAPPHGGMAAGVDRI
CCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHH
VMLLCGTTNLREISLFPMNQQAMDLLMGAPSEATTKQLRELHIKPNLPNK
HHHHHCCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHEECCCCCCCC
>Mature Secondary Structure
MHRYRTHTCGALRDSNIGETVRLSGWCHRIRDHGGVLFVDLRDHYGITQCVVDPDSKAFG
CCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCHHH
LAEKLRSEWVVRMEGKVRHRPEGTENPELPTGQVELYVADIEVLGPAAELPLPVFGEQDY
HHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCHHHCCCCCCCCCCC
PEDIRLKYRFLDLRRDRLHQNIMTRGAIIDSMRRRMKEQGFFEFQTPILTASSPEGARDF
CHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEECCCCCCCHHC
LVPSRIHPGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVEQEDVFAAMEPVITGVFEDFAKGKPVTKGWPRIPFAEALRKYGTDKPDLRNPLEMQ
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHH
DVSEHFRGSGFKVFARMLEDPKNQVWAIPAPGGGSRAFCDRMNSWAQGEGQPGLGYIMWR
HHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCHHCCCCCCCCCEEEEEE
EGGEGAGPLANNIGPERTAAIRSQLGTKEGDAAFFVAGDPDKFWKFAGLARTKVGEELNL
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCC
IDKDRFALAWIVDFPMYEYNEDDKKVDFSHNPFSMPQGGLEALTTKDPLTIKAFQYDIAC
CCCCCEEEEEEEECCCCCCCCCCCEECCCCCCCCCCCCCHHHHCCCCCEEEEEEEEEEEE
NGYEIASGGIRNHKPEAMVKAFEIAGYGEQEVVDRFGGMYRAFQYGAPPHGGMAAGVDRI
CCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHH
VMLLCGTTNLREISLFPMNQQAMDLLMGAPSEATTKQLRELHIKPNLPNK
HHHHHCCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]