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The map label for this gene is rlmB [H]

Identifier: 146340002

GI number: 146340002

Start: 3205782

End: 3206675

Strand: Reverse

Name: rlmB [H]

Synonym: BRADO3007

Alternate gene names: 146340002

Gene position: 3206675-3205782 (Counterclockwise)

Preceding gene: 146340003

Following gene: 146339999

Centisome position: 43.0

GC content: 68.9

Gene sequence:

>894_bases
TTGGCGGTGCCAAATTGCCAGAATCCGCCGGCCATGACAGAAGCTCGGGTCATGAGCGAACGCGACCGAAAGTCCCCATT
CCGCCGCCAAGGTGGTAAATCCGCCGGCAAGTTCACCGGCAAACCGCGCGGTTTCGACCGGCCGCACGGCCGCTGGGAGC
GCGACTCGGACGCGGACGGTCCCGTCATTCTCTATGGCTGGCACACGGTCAGCCTGGCGCTGGCCAACCCCAAGCGCCGC
ATCCGCAAACTGTACCTGACGGAAAACGCCGCGCGGAGGCTTGCCGACGAGCACATCGAGACGCGGGTGACGCCGGAAAT
CGTACGTCCGAGCCAGATCGACCAGCGACTCGGTCCCGACGCCGTGCATCAGGGCCTGCTCGCGGAGGCCGATCCCCTGC
CCTCGCCCGACATCGACACGCTGGCCCCCGAGGGGATGATCCTGGTGCTCGACCAGATCACCGATCCGCACAATGTCGGC
GCTATCCTGCGCTCGGCGGCGGCGTTCGACGTCAAGGCAATCGTGACCACCGCGCGGCACAGCCCGGAGGCGACCGGCGT
GCTCGCCAAATCGGCGTCGGGGGCGCTCGAACTGGTGCCGATGATCACGGTGCAGAACCTCGCCCGCGCCCTCACCCGGC
TCAACGAGCGCGGCTTCCAGACCGTGGGGCTCGACAGCGCCGGCGACACCGACATCGCCGCGGTGACGCTGCGGCAGCCG
CTGGCGTTGGTGCTCGGCGCCGAGGGCAAGGGCCTGCGGCAGCTGACGCGCGAGACCTGCAGCGCGGTGGCGCGGCTCGA
CATGCCCGGCGCCATCAAGAGCCTGAACGTCTCCAATGCGGCCGTGCTCGCACTCTATGTCGGAGCCAGCCGGCTGGGAT
TGATGGGCGGCTGA

Upstream 100 bases:

>100_bases
CGGTAAGGCCGCGGGCCCGATGAGGGCTGCCGCGCGGCCGGGGCCCTTATGATCGAACCGGCACCGTAAAGTCAACCAAG
CGGCGACGAATTTTCCCGCT

Downstream 100 bases:

>100_bases
CGCGACGTTCGATCGAAGGCGAAGGGAGCGCAATAGCAAACGCCCGCGCGCGGCATGCGAGCGGGCGTGTGCGGATGGTG
TCAGACCGGCTGATCAGTAA

Product: putative rRNA methylase 23S rRNA (guanosine-2'-O-)-methyltransferase rlmB-like

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MAVPNCQNPPAMTEARVMSERDRKSPFRRQGGKSAGKFTGKPRGFDRPHGRWERDSDADGPVILYGWHTVSLALANPKRR
IRKLYLTENAARRLADEHIETRVTPEIVRPSQIDQRLGPDAVHQGLLAEADPLPSPDIDTLAPEGMILVLDQITDPHNVG
AILRSAAAFDVKAIVTTARHSPEATGVLAKSASGALELVPMITVQNLARALTRLNERGFQTVGLDSAGDTDIAAVTLRQP
LALVLGAEGKGLRQLTRETCSAVARLDMPGAIKSLNVSNAAVLALYVGASRLGLMGG

Sequences:

>Translated_297_residues
MAVPNCQNPPAMTEARVMSERDRKSPFRRQGGKSAGKFTGKPRGFDRPHGRWERDSDADGPVILYGWHTVSLALANPKRR
IRKLYLTENAARRLADEHIETRVTPEIVRPSQIDQRLGPDAVHQGLLAEADPLPSPDIDTLAPEGMILVLDQITDPHNVG
AILRSAAAFDVKAIVTTARHSPEATGVLAKSASGALELVPMITVQNLARALTRLNERGFQTVGLDSAGDTDIAAVTLRQP
LALVLGAEGKGLRQLTRETCSAVARLDMPGAIKSLNVSNAAVLALYVGASRLGLMGG
>Mature_296_residues
AVPNCQNPPAMTEARVMSERDRKSPFRRQGGKSAGKFTGKPRGFDRPHGRWERDSDADGPVILYGWHTVSLALANPKRRI
RKLYLTENAARRLADEHIETRVTPEIVRPSQIDQRLGPDAVHQGLLAEADPLPSPDIDTLAPEGMILVLDQITDPHNVGA
ILRSAAAFDVKAIVTTARHSPEATGVLAKSASGALELVPMITVQNLARALTRLNERGFQTVGLDSAGDTDIAAVTLRQPL
ALVLGAEGKGLRQLTRETCSAVARLDMPGAIKSLNVSNAAVLALYVGASRLGLMGG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=260, Percent_Identity=31.5384615384615, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1790623, Length=234, Percent_Identity=34.6153846153846, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1788935, Length=139, Percent_Identity=30.2158273381295, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17555908, Length=245, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324775, Length=291, Percent_Identity=28.8659793814433, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31820; Mature: 31689

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVPNCQNPPAMTEARVMSERDRKSPFRRQGGKSAGKFTGKPRGFDRPHGRWERDSDADG
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PVILYGWHTVSLALANPKRRIRKLYLTENAARRLADEHIETRVTPEIVRPSQIDQRLGPD
CEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHCCHH
AVHQGLLAEADPLPSPDIDTLAPEGMILVLDQITDPHNVGAILRSAAAFDVKAIVTTARH
HHHHCHHCCCCCCCCCCCHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SPEATGVLAKSASGALELVPMITVQNLARALTRLNERGFQTVGLDSAGDTDIAAVTLRQP
CCCHHHEEHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEHHCC
LALVLGAEGKGLRQLTRETCSAVARLDMPGAIKSLNVSNAAVLALYVGASRLGLMGG
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCHHHHCCCCC
>Mature Secondary Structure 
AVPNCQNPPAMTEARVMSERDRKSPFRRQGGKSAGKFTGKPRGFDRPHGRWERDSDADG
CCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PVILYGWHTVSLALANPKRRIRKLYLTENAARRLADEHIETRVTPEIVRPSQIDQRLGPD
CEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHCCHH
AVHQGLLAEADPLPSPDIDTLAPEGMILVLDQITDPHNVGAILRSAAAFDVKAIVTTARH
HHHHCHHCCCCCCCCCCCHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SPEATGVLAKSASGALELVPMITVQNLARALTRLNERGFQTVGLDSAGDTDIAAVTLRQP
CCCHHHEEHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEHHCC
LALVLGAEGKGLRQLTRETCSAVARLDMPGAIKSLNVSNAAVLALYVGASRLGLMGG
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]