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The map label for this gene is thrS [H]

Identifier: 146339967

GI number: 146339967

Start: 3173141

End: 3175207

Strand: Reverse

Name: thrS [H]

Synonym: BRADO2971

Alternate gene names: 146339967

Gene position: 3175207-3173141 (Counterclockwise)

Preceding gene: 146339971

Following gene: 146339965

Centisome position: 42.58

GC content: 64.88

Gene sequence:

>2067_bases
ATGCCCGACTCGAAATCCCCCGATTCGAAATCCGACCAGTCCAAGTCCGGCTTCCAGTACAGCCTCAGCAACCTCAAGCC
CGCCGCTCCGGTCGAGAAGATCACCATCAGCTTCCCGGACGGCGCCAAGCGCGAATATCCCAGGGGCACCACCGGCTTGG
ACATCGCCAAGGGCATTTCGCCCTCGCTCGCCAAGCGCACGGTCGCAATGGCGCTGAACGGCGACGTCGCCGACCTCAAC
GATCCGATCAACGAGGATGCCAGGCTCGAGCTCGTCGACCGCGACGATCCACGCGCGCTGGAGCTGATCCGGCACGACTG
CGCGCACGTGCTCGCGGAAGCGGTGCAGGCGCTGTGGCCGGGCACCCAGGTCACGATCGGCCCGGTCATCGAGAACGGCT
TCTATTACGACTTCTTCCGCAACGAGCCGTTCACGCCCGAGGACTTTGCCGCGATCGAGAAGAAGATGCGCGAGATCATC
GGCCGCGACAAGCCCTTCACCAAGGAGGTCTGGGACCGCGAGAAGACCAAGCAGGTGTTCCGCGACAAGGGCGAGGCGTT
CAAGGTCGAGCTGGTCGATGCCATTCCCGGCAACGAGCCGATCAAGATCTACTATCAGGGCGACTGGTTCGATTTGTGCC
GCGGCCCGCACATGACCTCGACGGGGAAGATCGGCAACGCGTTCAAGCTGATGAAGGTGGCGGGCGCCTATTGGCGCGGC
GATTCCAACAACCCGATGCTGACCCGCATCTACGGCACCGCCTTCGCCAAGCAGGAGCAGCTCGACGCCTATCTGAAGCA
GATCGAGGAAGCCGAGAAGCGTGACCACCGCAAGCTCGGTCGCGAGCTCGACCTGTTCCACTTCCAGGAGGAAGGCCCTG
GCGTCGTGTTCTGGCACCCCAAGGGCTGGAGCATCTTCCAGGCGCTGATCGCCTATATGCGCAGGCGTCTTGCCGGCGAC
TATGACGAGGTGAATGCGCCGCAGATCCTCGACAAGTCGCTGTGGGAGACCTCCGGCCACTGGAACTGGTACCGCGAGAA
CATGTTCGCGGCGCAGTCCGCCGGCGACGAGGCCGAGGACAAGCGCTGGTTCGCGCTGAAGCCGATGAACTGCCCGGGTC
ACGTGCAGATCTTCAAGCATGGCCTGAAGAGCTACCGCGACCTGCCGATGCGGCTCGCCGAGTTCGGCGTCGTGCACCGC
TACGAGGCCTCCGGCGCCATGCACGGGTTGATGCGCGTGCGCGGCTTCACCCAGGACGACGCGCATGTGTTCTGCACGGA
AGACCAGCTCGCCGAGGAGTGCCTGAAGATCAACGATCTGATCCTCTCGACCTACTCCGACTTCGGGTTCGAGGGCGAGT
TGACGGTCAAGCTGTCGACCCGGCCGGAGAAGCGCGTCGGCACCGACGAAATGTGGGATCATGCCGAGCGCGTGATGGCG
ACCGTGCTGAACGAGATCAAGGCGCAGAACAACCACATCCGCACCGAGATCAATCCGGGCGAAGGCGCGTTCTACGGGCC
GAAGTTCGAATACGTGCTGCGCGACGCCATCGGCCGCGACTGGCAGTGCGGCACCACGCAGGTCGACTTCAACCTGCCGG
AGCGGTTCGGCGCGTTCTACATCGACGCCGACGGCGCCAAGAAGGCGCCGGTGATGGTGCATCGGGCGATCTGCGGCTCG
ATGGAGCGCTTCATCGGCATCCTGATCGAGCACTATGCCGGCAGCTTCCCGCTGTGGCTGGCGCCGACCCAGGTCGTCGT
CACCACCATCACCTCCGAAGGCGACGAATACGCCAAGCAGGTGCTGGCGGCGCTGCGCCGGGCGGGTCTTCGGGCCGAGA
TCGATCTTCGCAACGAGAAGATCAACTACAAGGTCCGCGAGCATTCGCTGCAGAAGATCCCGGCGCTGCTCGTGGTCGGC
AAGAAGGAGGCCGAGACGCATTCGGTCTCCGTCCGCCGCCTCGGCCGCGACGGCCAGACGGTGATGCCGACCGCGGATGC
CATCGCCGCGCTCGTCGACGAGGCGACGCCGCCGGACGTGCGACGCAGGCAGCGCGAGGCGGCCTGA

Upstream 100 bases:

>100_bases
CCCGTCTTCCCCGAGGGTTGCAGCCTGGAAATCCCCTGCTATACCCCAGGCTCCCGCTTACCTGCGCTCGTTCGCAGGAG
TGAGCTAACGGAGAGAGAAA

Downstream 100 bases:

>100_bases
GCGAAGGCCGCTTCGTAGCCCGCATGAGCGCAGCGAGATGCGGGTATCCGCAACAATGCGTGAGATCCCGGATCTCGCTC
CCGCCGCAGCCCTGACGGGC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MPDSKSPDSKSDQSKSGFQYSLSNLKPAAPVEKITISFPDGAKREYPRGTTGLDIAKGISPSLAKRTVAMALNGDVADLN
DPINEDARLELVDRDDPRALELIRHDCAHVLAEAVQALWPGTQVTIGPVIENGFYYDFFRNEPFTPEDFAAIEKKMREII
GRDKPFTKEVWDREKTKQVFRDKGEAFKVELVDAIPGNEPIKIYYQGDWFDLCRGPHMTSTGKIGNAFKLMKVAGAYWRG
DSNNPMLTRIYGTAFAKQEQLDAYLKQIEEAEKRDHRKLGRELDLFHFQEEGPGVVFWHPKGWSIFQALIAYMRRRLAGD
YDEVNAPQILDKSLWETSGHWNWYRENMFAAQSAGDEAEDKRWFALKPMNCPGHVQIFKHGLKSYRDLPMRLAEFGVVHR
YEASGAMHGLMRVRGFTQDDAHVFCTEDQLAEECLKINDLILSTYSDFGFEGELTVKLSTRPEKRVGTDEMWDHAERVMA
TVLNEIKAQNNHIRTEINPGEGAFYGPKFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFYIDADGAKKAPVMVHRAICGS
MERFIGILIEHYAGSFPLWLAPTQVVVTTITSEGDEYAKQVLAALRRAGLRAEIDLRNEKINYKVREHSLQKIPALLVVG
KKEAETHSVSVRRLGRDGQTVMPTADAIAALVDEATPPDVRRRQREAA

Sequences:

>Translated_688_residues
MPDSKSPDSKSDQSKSGFQYSLSNLKPAAPVEKITISFPDGAKREYPRGTTGLDIAKGISPSLAKRTVAMALNGDVADLN
DPINEDARLELVDRDDPRALELIRHDCAHVLAEAVQALWPGTQVTIGPVIENGFYYDFFRNEPFTPEDFAAIEKKMREII
GRDKPFTKEVWDREKTKQVFRDKGEAFKVELVDAIPGNEPIKIYYQGDWFDLCRGPHMTSTGKIGNAFKLMKVAGAYWRG
DSNNPMLTRIYGTAFAKQEQLDAYLKQIEEAEKRDHRKLGRELDLFHFQEEGPGVVFWHPKGWSIFQALIAYMRRRLAGD
YDEVNAPQILDKSLWETSGHWNWYRENMFAAQSAGDEAEDKRWFALKPMNCPGHVQIFKHGLKSYRDLPMRLAEFGVVHR
YEASGAMHGLMRVRGFTQDDAHVFCTEDQLAEECLKINDLILSTYSDFGFEGELTVKLSTRPEKRVGTDEMWDHAERVMA
TVLNEIKAQNNHIRTEINPGEGAFYGPKFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFYIDADGAKKAPVMVHRAICGS
MERFIGILIEHYAGSFPLWLAPTQVVVTTITSEGDEYAKQVLAALRRAGLRAEIDLRNEKINYKVREHSLQKIPALLVVG
KKEAETHSVSVRRLGRDGQTVMPTADAIAALVDEATPPDVRRRQREAA
>Mature_687_residues
PDSKSPDSKSDQSKSGFQYSLSNLKPAAPVEKITISFPDGAKREYPRGTTGLDIAKGISPSLAKRTVAMALNGDVADLND
PINEDARLELVDRDDPRALELIRHDCAHVLAEAVQALWPGTQVTIGPVIENGFYYDFFRNEPFTPEDFAAIEKKMREIIG
RDKPFTKEVWDREKTKQVFRDKGEAFKVELVDAIPGNEPIKIYYQGDWFDLCRGPHMTSTGKIGNAFKLMKVAGAYWRGD
SNNPMLTRIYGTAFAKQEQLDAYLKQIEEAEKRDHRKLGRELDLFHFQEEGPGVVFWHPKGWSIFQALIAYMRRRLAGDY
DEVNAPQILDKSLWETSGHWNWYRENMFAAQSAGDEAEDKRWFALKPMNCPGHVQIFKHGLKSYRDLPMRLAEFGVVHRY
EASGAMHGLMRVRGFTQDDAHVFCTEDQLAEECLKINDLILSTYSDFGFEGELTVKLSTRPEKRVGTDEMWDHAERVMAT
VLNEIKAQNNHIRTEINPGEGAFYGPKFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFYIDADGAKKAPVMVHRAICGSM
ERFIGILIEHYAGSFPLWLAPTQVVVTTITSEGDEYAKQVLAALRRAGLRAEIDLRNEKINYKVREHSLQKIPALLVVGK
KEAETHSVSVRRLGRDGQTVMPTADAIAALVDEATPPDVRRRQREAA

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=624, Percent_Identity=42.6282051282051, Blast_Score=476, Evalue=1e-134,
Organism=Homo sapiens, GI38570111, Length=607, Percent_Identity=41.0214168039539, Blast_Score=458, Evalue=1e-129,
Organism=Homo sapiens, GI20070344, Length=635, Percent_Identity=38.4251968503937, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI1788013, Length=626, Percent_Identity=50.9584664536741, Blast_Score=672, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984192, Length=630, Percent_Identity=40.4761904761905, Blast_Score=462, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71984184, Length=630, Percent_Identity=40.4761904761905, Blast_Score=462, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322112, Length=647, Percent_Identity=40.4945904173107, Blast_Score=460, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322654, Length=396, Percent_Identity=35.6060606060606, Blast_Score=276, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24583842, Length=645, Percent_Identity=40.3100775193798, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI28574141, Length=645, Percent_Identity=40.3100775193798, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24583839, Length=644, Percent_Identity=40.0621118012422, Blast_Score=474, Evalue=1e-133,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 77926; Mature: 77795

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDSKSPDSKSDQSKSGFQYSLSNLKPAAPVEKITISFPDGAKREYPRGTTGLDIAKGIS
CCCCCCCCCCCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCC
PSLAKRTVAMALNGDVADLNDPINEDARLELVDRDDPRALELIRHDCAHVLAEAVQALWP
HHHHHHEEEEEECCCHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
GTQVTIGPVIENGFYYDFFRNEPFTPEDFAAIEKKMREIIGRDKPFTKEVWDREKTKQVF
CCEEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHHH
RDKGEAFKVELVDAIPGNEPIKIYYQGDWFDLCRGPHMTSTGKIGNAFKLMKVAGAYWRG
HCCCCEEEEEEEECCCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCC
DSNNPMLTRIYGTAFAKQEQLDAYLKQIEEAEKRDHRKLGRELDLFHFQEEGPGVVFWHP
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECC
KGWSIFQALIAYMRRRLAGDYDEVNAPQILDKSLWETSGHWNWYRENMFAAQSAGDEAED
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCC
KRWFALKPMNCPGHVQIFKHGLKSYRDLPMRLAEFGVVHRYEASGAMHGLMRVRGFTQDD
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCCC
AHVFCTEDQLAEECLKINDLILSTYSDFGFEGELTVKLSTRPEKRVGTDEMWDHAERVMA
CEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
TVLNEIKAQNNHIRTEINPGEGAFYGPKFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFY
HHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCHHHHCEEE
IDADGAKKAPVMVHRAICGSMERFIGILIEHYAGSFPLWLAPTQVVVTTITSEGDEYAKQ
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEECCCCHHHHHH
VLAALRRAGLRAEIDLRNEKINYKVREHSLQKIPALLVVGKKEAETHSVSVRRLGRDGQT
HHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCCCE
VMPTADAIAALVDEATPPDVRRRQREAA
ECCHHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure 
PDSKSPDSKSDQSKSGFQYSLSNLKPAAPVEKITISFPDGAKREYPRGTTGLDIAKGIS
CCCCCCCCCCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCC
PSLAKRTVAMALNGDVADLNDPINEDARLELVDRDDPRALELIRHDCAHVLAEAVQALWP
HHHHHHEEEEEECCCHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
GTQVTIGPVIENGFYYDFFRNEPFTPEDFAAIEKKMREIIGRDKPFTKEVWDREKTKQVF
CCEEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHHH
RDKGEAFKVELVDAIPGNEPIKIYYQGDWFDLCRGPHMTSTGKIGNAFKLMKVAGAYWRG
HCCCCEEEEEEEECCCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCC
DSNNPMLTRIYGTAFAKQEQLDAYLKQIEEAEKRDHRKLGRELDLFHFQEEGPGVVFWHP
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECC
KGWSIFQALIAYMRRRLAGDYDEVNAPQILDKSLWETSGHWNWYRENMFAAQSAGDEAED
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCC
KRWFALKPMNCPGHVQIFKHGLKSYRDLPMRLAEFGVVHRYEASGAMHGLMRVRGFTQDD
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCCC
AHVFCTEDQLAEECLKINDLILSTYSDFGFEGELTVKLSTRPEKRVGTDEMWDHAERVMA
CEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
TVLNEIKAQNNHIRTEINPGEGAFYGPKFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFY
HHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCHHHHCEEE
IDADGAKKAPVMVHRAICGSMERFIGILIEHYAGSFPLWLAPTQVVVTTITSEGDEYAKQ
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEECCCCHHHHHH
VLAALRRAGLRAEIDLRNEKINYKVREHSLQKIPALLVVGKKEAETHSVSVRRLGRDGQT
HHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCCCE
VMPTADAIAALVDEATPPDVRRRQREAA
ECCHHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA