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The map label for this gene is groEL [H]

Identifier: 146339890

GI number: 146339890

Start: 3093555

End: 3095195

Strand: Reverse

Name: groEL [H]

Synonym: BRADO2892

Alternate gene names: 146339890

Gene position: 3095195-3093555 (Counterclockwise)

Preceding gene: 146339891

Following gene: 146339888

Centisome position: 41.51

GC content: 65.75

Gene sequence:

>1641_bases
ATGTCAGCGAAAGACGTAAAGTTCGGCGTCGAAGCCCGCGACCGCATGCTGCGCGGCGTGGACATTCTCGCCAATGCCGT
GAAGGTCACGCTCGGTCCGAAGGGCCGCAACGTCGTCCTCGACAAGTCGTTCGGTGCGCCGCGCATCACCAAGGACGGCG
TCACCGTCGCCAAGGAGATCGAGCTCGACGACAAGTTCGAAAACATGGGCGCACAGATGGTGCGCGAGGTCGCTTCGAAG
TCCGCTGACGCGGCTGGCGACGGCACCACCACCGCGACCGTGCTGGCCCAGGCCATCGTGCGCGAAGGCGCCAAGGCGGT
TGCCGCCGGCATGAACCCGATGGATCTGAAGCGCGGCATCGACCTCGCGGTCGAGGCGGTCGTGGCCGATCTCGTCAAGA
ACTCCAAGAAGGTCACGTCGAACGACGAGATCGCCCAGGTCGGCACCATCTCCGCCAACGGCGACTCCGAGATCGGCAAG
TTCCTCGCCGACGCCATGAAGAAGGTCGGCAACGAGGGTGTCATCACCGTCGAGGAAGCCAAGTCGCTCGAGACCGAGCT
CGACGTCGTCGAGGGCATGCAGTTCGATCGCGGCTACATCTCTCCCTACTTCGTCACCAACGCCGACAAGATGCGCGTTG
AGATGGACGACGCCTACATCCTCATCAACGAGAAGAAGCTGTCGTCGCTGAACGAGCTGCTGCCGCTGCTCGAGGCCGTG
GTGCAGACCGGCAAGCCGCTGGTGATCGTCGCCGAGGACGTCGAGGGCGAGGCTCTGGCTACGCTCGTCGTGAACCGTCT
GCGTGGCGGCCTGAAGGTTGCCGCCGTCAAGGCGCCGGGCTTCGGCGACCGCCGCAAGGCCATGCTGCAGGACATCGCGA
TCCTGACCGGCGGCCAGGCGATCTCGGAAGACCTCGGCATCAAGCTCGAGAACGTCAACCTCTCCATGCTCGGCCGCGCC
AAGAAGGTGATGATCGACAAGGAGAACACCACGATCGTCAACGGCGCCGGCAAGAAGGCCGACATCGAGGCGCGCGTGGC
GCAGATCAAGGCGCAGATCGAGGAGACCACCTCGGACTACGACCGTGAGAAGCTGCAGGAGCGTCTGGCCAAGCTCGCCG
GCGGCGTCGCGGTGATCCGCGTCGGCGGCGCGACCGAGGTCGAGGTGAAGGAGCGCAAGGATCGCGTTGACGACGCGATG
CATGCGACCCGCGCGGCTGTCGAGGAAGGCATCCTGCCGGGCGGCGGCGTCGCCCTGCTCCGTGCCTCCGAGCAGCTCAA
GGGTGTCCGCACCAAGAACGAGGACCAGAAGACCGGTGTCGAGATCGTCCGCAAGGCGCTCTCCGCTCCGGCCCGCCAGA
TCGCCATCAACGCGGGCGAGGACGGTTCGGTCATCGTCGGCAAGATCCTCGAGAAGGAGCAGTATGCCTACGGCTTCGAC
TCGCAGTCGGGCGACTACGTGAACATGGTGTCCAAGGGCATCATCGACCCGACCAAGGTCGTGCGCACCGCGATCCAGAA
CGCCGCCTCCGTGGCGAGCCTCTTGATCACGACCGAGGCGATGGTCGCCGAGCTGCCGAAGAAGGCCGCCGCCGGCCCCG
CGATGCCTCCGGGCGGCGGCATGGGCGGCATGGACTTCTAA

Upstream 100 bases:

>100_bases
TGAGGCCTCAACCACCTCCTCATCTCGTCCTTCGAGACGCCCGCGACGTGCGCGGGCTCCTCGGGACGAGGACCTCGATC
AAAACTCAGGGAAACCCAAT

Downstream 100 bases:

>100_bases
GTCCGGCTTCCATCTGATCGTTGCAAAACCCCGGCAGCAATGCCGGGGTTTTCGTTTGTGCGGGAATTCGCCCGCTTGCG
CTGGGCACAGCGCGTAGCCC

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein 2; Protein Cpn60 2 [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSAKDVKFGVEARDRMLRGVDILANAVKVTLGPKGRNVVLDKSFGAPRITKDGVTVAKEIELDDKFENMGAQMVREVASK
SADAAGDGTTTATVLAQAIVREGAKAVAAGMNPMDLKRGIDLAVEAVVADLVKNSKKVTSNDEIAQVGTISANGDSEIGK
FLADAMKKVGNEGVITVEEAKSLETELDVVEGMQFDRGYISPYFVTNADKMRVEMDDAYILINEKKLSSLNELLPLLEAV
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQAISEDLGIKLENVNLSMLGRA
KKVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAM
HATRAAVEEGILPGGGVALLRASEQLKGVRTKNEDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKILEKEQYAYGFD
SQSGDYVNMVSKGIIDPTKVVRTAIQNAASVASLLITTEAMVAELPKKAAAGPAMPPGGGMGGMDF

Sequences:

>Translated_546_residues
MSAKDVKFGVEARDRMLRGVDILANAVKVTLGPKGRNVVLDKSFGAPRITKDGVTVAKEIELDDKFENMGAQMVREVASK
SADAAGDGTTTATVLAQAIVREGAKAVAAGMNPMDLKRGIDLAVEAVVADLVKNSKKVTSNDEIAQVGTISANGDSEIGK
FLADAMKKVGNEGVITVEEAKSLETELDVVEGMQFDRGYISPYFVTNADKMRVEMDDAYILINEKKLSSLNELLPLLEAV
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQAISEDLGIKLENVNLSMLGRA
KKVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAM
HATRAAVEEGILPGGGVALLRASEQLKGVRTKNEDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKILEKEQYAYGFD
SQSGDYVNMVSKGIIDPTKVVRTAIQNAASVASLLITTEAMVAELPKKAAAGPAMPPGGGMGGMDF
>Mature_545_residues
SAKDVKFGVEARDRMLRGVDILANAVKVTLGPKGRNVVLDKSFGAPRITKDGVTVAKEIELDDKFENMGAQMVREVASKS
ADAAGDGTTTATVLAQAIVREGAKAVAAGMNPMDLKRGIDLAVEAVVADLVKNSKKVTSNDEIAQVGTISANGDSEIGKF
LADAMKKVGNEGVITVEEAKSLETELDVVEGMQFDRGYISPYFVTNADKMRVEMDDAYILINEKKLSSLNELLPLLEAVV
QTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQAISEDLGIKLENVNLSMLGRAK
KVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAMH
ATRAAVEEGILPGGGVALLRASEQLKGVRTKNEDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKILEKEQYAYGFDS
QSGDYVNMVSKGIIDPTKVVRTAIQNAASVASLLITTEAMVAELPKKAAAGPAMPPGGGMGGMDF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=527, Percent_Identity=56.7362428842505, Blast_Score=595, Evalue=1e-170,
Organism=Homo sapiens, GI31542947, Length=527, Percent_Identity=56.7362428842505, Blast_Score=595, Evalue=1e-170,
Organism=Homo sapiens, GI5453607, Length=547, Percent_Identity=23.400365630713, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790586, Length=531, Percent_Identity=65.9133709981168, Blast_Score=691, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=529, Percent_Identity=55.1984877126654, Blast_Score=582, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI193210679, Length=211, Percent_Identity=54.9763033175355, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=57.7946768060836, Blast_Score=608, Evalue=1e-175,
Organism=Saccharomyces cerevisiae, GI6322524, Length=189, Percent_Identity=25.9259259259259, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322350, Length=543, Percent_Identity=22.8360957642726, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=59.2030360531309, Blast_Score=617, Evalue=1e-177,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=59.2030360531309, Blast_Score=617, Evalue=1e-177,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=54.6666666666667, Blast_Score=576, Evalue=1e-164,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=54.6666666666667, Blast_Score=576, Evalue=1e-164,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=54.6666666666667, Blast_Score=576, Evalue=1e-164,
Organism=Drosophila melanogaster, GI17864606, Length=555, Percent_Identity=45.945945945946, Blast_Score=486, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24584129, Length=537, Percent_Identity=36.1266294227188, Blast_Score=341, Evalue=9e-94,
Organism=Drosophila melanogaster, GI19921262, Length=537, Percent_Identity=36.1266294227188, Blast_Score=341, Evalue=9e-94,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57798; Mature: 57667

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKDVKFGVEARDRMLRGVDILANAVKVTLGPKGRNVVLDKSFGAPRITKDGVTVAKEI
CCCCCCEECHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEEC
ELDDKFENMGAQMVREVASKSADAAGDGTTTATVLAQAIVREGAKAVAAGMNPMDLKRGI
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHCH
DLAVEAVVADLVKNSKKVTSNDEIAQVGTISANGDSEIGKFLADAMKKVGNEGVITVEEA
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEHHH
KSLETELDVVEGMQFDRGYISPYFVTNADKMRVEMDDAYILINEKKLSSLNELLPLLEAV
HHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHH
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQA
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHH
ISEDLGIKLENVNLSMLGRAKKVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDY
HHHHHCEEEECCCHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAMHATRAAVEEGILPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
RASEQLKGVRTKNEDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKILEKEQYAYGFD
ECHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHHCCCCC
SQSGDYVNMVSKGIIDPTKVVRTAIQNAASVASLLITTEAMVAELPKKAAAGPAMPPGGG
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCC
MGGMDF
CCCCCC
>Mature Secondary Structure 
SAKDVKFGVEARDRMLRGVDILANAVKVTLGPKGRNVVLDKSFGAPRITKDGVTVAKEI
CCCCCEECHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEEC
ELDDKFENMGAQMVREVASKSADAAGDGTTTATVLAQAIVREGAKAVAAGMNPMDLKRGI
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHCH
DLAVEAVVADLVKNSKKVTSNDEIAQVGTISANGDSEIGKFLADAMKKVGNEGVITVEEA
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEHHH
KSLETELDVVEGMQFDRGYISPYFVTNADKMRVEMDDAYILINEKKLSSLNELLPLLEAV
HHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHH
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQA
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHH
ISEDLGIKLENVNLSMLGRAKKVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDY
HHHHHCEEEECCCHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAMHATRAAVEEGILPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
RASEQLKGVRTKNEDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKILEKEQYAYGFD
ECHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHHCCCCC
SQSGDYVNMVSKGIIDPTKVVRTAIQNAASVASLLITTEAMVAELPKKAAAGPAMPPGGG
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCC
MGGMDF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8101485; 12597275 [H]