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The map label for this gene is dctA [H]

Identifier: 146339838

GI number: 146339838

Start: 3041086

End: 3042417

Strand: Direct

Name: dctA [H]

Synonym: BRADO2839

Alternate gene names: 146339838

Gene position: 3041086-3042417 (Clockwise)

Preceding gene: 146339837

Following gene: 146339842

Centisome position: 40.78

GC content: 65.77

Gene sequence:

>1332_bases
ATGGCGGCGACGGATGTGAGCATGGCGGGGACGGCGTCCCCGAAGGGCAGCCTGTTCGGCTCGCTGTTCTTTCAGGTCGT
GGCGGCGCTCGCGATCGGCATCCTGATCGGCGTCGTCGCGCCCGAGACCGCCCAGGGCCTGAAATTCTTCAGCGACGCCT
TCCTGCGCCTGATCGCGATGGTCGTGGCGCCGATCGTGTTCTGCGTCGTCGTCCATGGCATCGCCAGTGCCGGCGACCTC
AGAAAGGTCGGCCGCGTCGGCGTCAAGTCGCTGGTCTATTTCGAGGGCATGACGACGATCGCGCTGGCCTTCGGCCTCGT
TCTGGCGCTGCTGTTCGGCCCCGGCCATGGCATGAACATCGACGTCTCGAAGCTCGATGCCAAGCAGCTCGCGACCTTCG
CCGACAACGCCCACAAGCTGCAAGGTGGCGGCTTTGGCGGCTTCATCCTCAACATCATCCCGCCGACCGCTTTCGACGCC
TTCGCCCGTAACGACGTGCTGCAGGTGCTGTTCTTCGCGGTGATCTTCGGCGTCAGCCTTGCGCTGGTCGGCGAGCCGGG
CAAGCCCGTCGTCAACCTGATCGATGCCGTCGCCAAGGTGCTGTTCAAGGCGATGGGGCTGATCGTGCGCGTCGCGCCGC
TCGGCGTGCTCGGCGCCATCGGCTTCACCGTCGGCAAATACGGCATCGGCTCGTTGAAGCAGCTGCTGGCGCTGGTCGTG
CTGTTCTACGTCTCGGTGGCGCTGTTCGTCGTCATCGCGCTCGGCCTCGTGATGCGCATCGCCGGCCTCAGCCTGTTCAA
GTTCCTGAGCTACCTGAAGGAGGAGCTCCTGATCGTGCTGGCGACGGCGTCGTCCGATTCCGTGCTGCCGCAGATCATGC
GCAAACTCGAGGCGATGGGGGTGAAGCAGGAGGTCGTCGGCCTGGTCATCCCGACCGGCTATTCGTTCAACCTCGATGCG
TTCTCGATCTATCTGACGCTCGCCGTCGTGTTCATCGCCCAGGCCACCAACACGCCGCTGTCGGTCAGCGACCTCTTGCT
GGTGCTCGGCGTGTCGCTGGTGACGTCGAAGGGCGCGCATGGCGTGCCGGGCTCGGCGATCGTCATCCTCGCCGCAACGC
TCGGTGCCGTGCCGGCGATCCCCGCGATCGGCCTCGTGCTGGTGCTGTCGGTCGACTGGTTCGTCGGCATCGCCCGCGCC
GTCGGCAACCTGATCGGCAACTGCGTCGCCACCGTGGTCGTCGCCGCCTGGGAAAATGATCTCGATCATGCCAGGGCGAA
AGCCGTGCTCGATCGCGGAACGGCGGAGGAGGTAGAGTATCAGGCGGCGTGA

Upstream 100 bases:

>100_bases
TATTCGGGCGCAGCTGCGATTGCCTTTCATAGTCTCTCCCGCCAGAGTGACATTGTGACGCGTGTGGGGCGCGCACGTCT
GGTTTGACGAGGGGGATTCG

Downstream 100 bases:

>100_bases
TTGCCGACAGACGTGGTGGTCGCGATCTCGTAGGGTCTCACGCGCTGTTGCGTCGATCCCGCATGTCGCTGCGCTCATGC
GGGCTACTGGACGGCATCGT

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MAATDVSMAGTASPKGSLFGSLFFQVVAALAIGILIGVVAPETAQGLKFFSDAFLRLIAMVVAPIVFCVVVHGIASAGDL
RKVGRVGVKSLVYFEGMTTIALAFGLVLALLFGPGHGMNIDVSKLDAKQLATFADNAHKLQGGGFGGFILNIIPPTAFDA
FARNDVLQVLFFAVIFGVSLALVGEPGKPVVNLIDAVAKVLFKAMGLIVRVAPLGVLGAIGFTVGKYGIGSLKQLLALVV
LFYVSVALFVVIALGLVMRIAGLSLFKFLSYLKEELLIVLATASSDSVLPQIMRKLEAMGVKQEVVGLVIPTGYSFNLDA
FSIYLTLAVVFIAQATNTPLSVSDLLLVLGVSLVTSKGAHGVPGSAIVILAATLGAVPAIPAIGLVLVLSVDWFVGIARA
VGNLIGNCVATVVVAAWENDLDHARAKAVLDRGTAEEVEYQAA

Sequences:

>Translated_443_residues
MAATDVSMAGTASPKGSLFGSLFFQVVAALAIGILIGVVAPETAQGLKFFSDAFLRLIAMVVAPIVFCVVVHGIASAGDL
RKVGRVGVKSLVYFEGMTTIALAFGLVLALLFGPGHGMNIDVSKLDAKQLATFADNAHKLQGGGFGGFILNIIPPTAFDA
FARNDVLQVLFFAVIFGVSLALVGEPGKPVVNLIDAVAKVLFKAMGLIVRVAPLGVLGAIGFTVGKYGIGSLKQLLALVV
LFYVSVALFVVIALGLVMRIAGLSLFKFLSYLKEELLIVLATASSDSVLPQIMRKLEAMGVKQEVVGLVIPTGYSFNLDA
FSIYLTLAVVFIAQATNTPLSVSDLLLVLGVSLVTSKGAHGVPGSAIVILAATLGAVPAIPAIGLVLVLSVDWFVGIARA
VGNLIGNCVATVVVAAWENDLDHARAKAVLDRGTAEEVEYQAA
>Mature_442_residues
AATDVSMAGTASPKGSLFGSLFFQVVAALAIGILIGVVAPETAQGLKFFSDAFLRLIAMVVAPIVFCVVVHGIASAGDLR
KVGRVGVKSLVYFEGMTTIALAFGLVLALLFGPGHGMNIDVSKLDAKQLATFADNAHKLQGGGFGGFILNIIPPTAFDAF
ARNDVLQVLFFAVIFGVSLALVGEPGKPVVNLIDAVAKVLFKAMGLIVRVAPLGVLGAIGFTVGKYGIGSLKQLLALVVL
FYVSVALFVVIALGLVMRIAGLSLFKFLSYLKEELLIVLATASSDSVLPQIMRKLEAMGVKQEVVGLVIPTGYSFNLDAF
SIYLTLAVVFIAQATNTPLSVSDLLLVLGVSLVTSKGAHGVPGSAIVILAATLGAVPAIPAIGLVLVLSVDWFVGIARAV
GNLIGNCVATVVVAAWENDLDHARAKAVLDRGTAEEVEYQAA

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=458, Percent_Identity=24.4541484716157, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI21314632, Length=442, Percent_Identity=25.5656108597285, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI194239697, Length=463, Percent_Identity=21.3822894168467, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI66773030, Length=447, Percent_Identity=24.8322147651007, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI40254478, Length=428, Percent_Identity=24.2990654205607, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI4827012, Length=278, Percent_Identity=24.8201438848921, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI5032093, Length=419, Percent_Identity=25.5369928400955, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI223468566, Length=268, Percent_Identity=25, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI223468564, Length=268, Percent_Identity=25, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI301601644, Length=184, Percent_Identity=29.3478260869565, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI262359914, Length=397, Percent_Identity=23.9294710327456, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789947, Length=418, Percent_Identity=49.2822966507177, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1790514, Length=419, Percent_Identity=36.9928400954654, Blast_Score=276, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI71996953, Length=420, Percent_Identity=25, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17537407, Length=424, Percent_Identity=23.5849056603774, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI193206505, Length=439, Percent_Identity=24.1457858769932, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17541374, Length=398, Percent_Identity=24.1206030150754, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193206654, Length=431, Percent_Identity=22.969837587007, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71983106, Length=270, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71983099, Length=270, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI281360483, Length=446, Percent_Identity=26.6816143497758, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17137666, Length=446, Percent_Identity=26.6816143497758, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281360481, Length=446, Percent_Identity=26.6816143497758, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24583025, Length=429, Percent_Identity=22.3776223776224, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137668, Length=429, Percent_Identity=22.3776223776224, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24583023, Length=429, Percent_Identity=22.3776223776224, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45920; Mature: 45789

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCHHHHCCCCC
>Mature Secondary Structure 
AATDVSMAGTASPKGSLFGSLFFQVVAALAIGILIGVVAPETAQGLKFFSDAFLRLIAM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VVAPIVFCVVVHGIASAGDLRKVGRVGVKSLVYFEGMTTIALAFGLVLALLFGPGHGMNI
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCC
DVSKLDAKQLATFADNAHKLQGGGFGGFILNIIPPTAFDAFARNDVLQVLFFAVIFGVSL
CHHHHHHHHHHHHHCCCCEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALVGEPGKPVVNLIDAVAKVLFKAMGLIVRVAPLGVLGAIGFTVGKYGIGSLKQLLALVV
HEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LFYVSVALFVVIALGLVMRIAGLSLFKFLSYLKEELLIVLATASSDSVLPQIMRKLEAMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHCC
VKQEVVGLVIPTGYSFNLDAFSIYLTLAVVFIAQATNTPLSVSDLLLVLGVSLVTSKGAH
CHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
GVPGSAIVILAATLGAVPAIPAIGLVLVLSVDWFVGIARAVGNLIGNCVATVVVAAWEND
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDHARAKAVLDRGTAEEVEYQAA
HHHHHHHHHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]