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The map label for this gene is ddl

Identifier: 146339809

GI number: 146339809

Start: 3013456

End: 3014559

Strand: Reverse

Name: ddl

Synonym: BRADO2810

Alternate gene names: 146339809

Gene position: 3014559-3013456 (Counterclockwise)

Preceding gene: 146339810

Following gene: 146339808

Centisome position: 40.43

GC content: 68.21

Gene sequence:

>1104_bases
ATGACTTCAGAACGAATCCGTGTGGCGATCCTGTTCGGCGGGCGCTCGGCGGAGCATGACGTCTCACGCGCGTCGGCTGC
CAACATCTTTCGCTCGCTCGATGCCGGCCGCTACGCGCTGACGCTGATCGGCATCACCCATGACGGCCGCTGGGTGCTCG
CCGACGCCGTCAATGATGCGACCAGTGCGGCGCTGATCGTGCCTGCCGATGGCCCGCAAATCGTGCTGCTGCCTGCGGGC
CGGGGCCGCGCGCTCGCCATCGACGGCAGCGCTGCCGCCCCGCGCGAACTCGCCTTCGACGTGATCTTTCCGGTGCTGCA
TGGTCCGAACGGCGAGGACGGCACCGTGCAGGGCGCGCTCGAGCTCGCCGATGTCGCCTATGTCGGCGGGCGCGTGCTCG
GCTCGGCGGCGGCGATGGACAAGGACGTCGCCAAGCGGCTCTTGCGCGATGCCGGCCTGCCGATCGTTCCGTTCGTCACC
ATGACGGCTGCATCCCCGGTGAGCTACGACGACGCCGCCCGCGCGGTGGGCTCGTCCGAGCTGTTCGTCAAGCCCGCCAA
TCTCGGCTCCTCCGTCGGCATCTCCAAGACGCGCGACGCAGCCGAGTTCGAGGCCGCCTGCCAGCTTGCGTTGCGCTTCG
ATCGCAAGATCCTGATCGAGCGCTGCATCGCGCCGGTGCGCGAGATCGAATGCGCCGTGCTGGAGCATGCCGACGGCCAG
ATCAAAGCCTCGGAGCTTGGCGAGATCGTGCCGGCGAACAGCCACGGCTTCTACTCTTACGAAGCCAAATACACCGACGC
CAACGGCGCCGCGCTGCACGTCCCGGCGCAGGTCGAGCCGGCCGTCGCGCAGCGCATCCGCAAGATGGCAACCGAGGTGT
TCGGCGCGCTCTGCTGCGAGAGCCTCGCACGCGTGGACTTCTTCGTCAGGGGCGACGAGATCTACGTCAACGAGGTGAAC
ACCCTTCCCGGCTTCACCAATATCAGCATGTATCCGAAGATGTGGGAGGCCGCCGGCCTGCCCCAGCCCGCGCTGATGGA
CGAGCTGGTCGCGCACGCGCTGGCGCGGCATGCAAGGCTGCGGGAGCTGGCGTCGCAGCGGTGA

Upstream 100 bases:

>100_bases
ACGCGAGGTGTCGGATATCTGCCGGCAAGGCACGTCAATTGCCACGCGCCGGCCTCTCGCGCTACGGTCGTCACGTCGTG
GCTCAGCCTGGTGACGGACG

Downstream 100 bases:

>100_bases
GTCCTTTGACCGGCATGAGGATCATGCGTCGCTTTCCCGTCGCGCAAGTTATGATCACCGCACTCTGTCTGATTACCGCG
GCCGCGCCCGCGCGCGGCGA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MTSERIRVAILFGGRSAEHDVSRASAANIFRSLDAGRYALTLIGITHDGRWVLADAVNDATSAALIVPADGPQIVLLPAG
RGRALAIDGSAAAPRELAFDVIFPVLHGPNGEDGTVQGALELADVAYVGGRVLGSAAAMDKDVAKRLLRDAGLPIVPFVT
MTAASPVSYDDAARAVGSSELFVKPANLGSSVGISKTRDAAEFEAACQLALRFDRKILIERCIAPVREIECAVLEHADGQ
IKASELGEIVPANSHGFYSYEAKYTDANGAALHVPAQVEPAVAQRIRKMATEVFGALCCESLARVDFFVRGDEIYVNEVN
TLPGFTNISMYPKMWEAAGLPQPALMDELVAHALARHARLRELASQR

Sequences:

>Translated_367_residues
MTSERIRVAILFGGRSAEHDVSRASAANIFRSLDAGRYALTLIGITHDGRWVLADAVNDATSAALIVPADGPQIVLLPAG
RGRALAIDGSAAAPRELAFDVIFPVLHGPNGEDGTVQGALELADVAYVGGRVLGSAAAMDKDVAKRLLRDAGLPIVPFVT
MTAASPVSYDDAARAVGSSELFVKPANLGSSVGISKTRDAAEFEAACQLALRFDRKILIERCIAPVREIECAVLEHADGQ
IKASELGEIVPANSHGFYSYEAKYTDANGAALHVPAQVEPAVAQRIRKMATEVFGALCCESLARVDFFVRGDEIYVNEVN
TLPGFTNISMYPKMWEAAGLPQPALMDELVAHALARHARLRELASQR
>Mature_366_residues
TSERIRVAILFGGRSAEHDVSRASAANIFRSLDAGRYALTLIGITHDGRWVLADAVNDATSAALIVPADGPQIVLLPAGR
GRALAIDGSAAAPRELAFDVIFPVLHGPNGEDGTVQGALELADVAYVGGRVLGSAAAMDKDVAKRLLRDAGLPIVPFVTM
TAASPVSYDDAARAVGSSELFVKPANLGSSVGISKTRDAAEFEAACQLALRFDRKILIERCIAPVREIECAVLEHADGQI
KASELGEIVPANSHGFYSYEAKYTDANGAALHVPAQVEPAVAQRIRKMATEVFGALCCESLARVDFFVRGDEIYVNEVNT
LPGFTNISMYPKMWEAAGLPQPALMDELVAHALARHARLRELASQR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=367, Percent_Identity=45.7765667574932, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1786280, Length=343, Percent_Identity=30.6122448979592, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_BRASO (A4YRU4)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001204857.1
- ProteinModelPortal:   A4YRU4
- SMR:   A4YRU4
- STRING:   A4YRU4
- GeneID:   5118091
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO2810
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   CKAINAY
- ProtClustDB:   PRK01966
- BioCyc:   BSP376:BRADO2810-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 39043; Mature: 38912

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSERIRVAILFGGRSAEHDVSRASAANIFRSLDAGRYALTLIGITHDGRWVLADAVNDA
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEECCCCC
TSAALIVPADGPQIVLLPAGRGRALAIDGSAAAPRELAFDVIFPVLHGPNGEDGTVQGAL
CCEEEEEECCCCEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
ELADVAYVGGRVLGSAAAMDKDVAKRLLRDAGLPIVPFVTMTAASPVSYDDAARAVGSSE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCC
LFVKPANLGSSVGISKTRDAAEFEAACQLALRFDRKILIERCIAPVREIECAVLEHADGQ
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IKASELGEIVPANSHGFYSYEAKYTDANGAALHVPAQVEPAVAQRIRKMATEVFGALCCE
EEHHHCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
SLARVDFFVRGDEIYVNEVNTLPGFTNISMYPKMWEAAGLPQPALMDELVAHALARHARL
HHHHCEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RELASQR
HHHHHCC
>Mature Secondary Structure 
TSERIRVAILFGGRSAEHDVSRASAANIFRSLDAGRYALTLIGITHDGRWVLADAVNDA
CCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEECCCCC
TSAALIVPADGPQIVLLPAGRGRALAIDGSAAAPRELAFDVIFPVLHGPNGEDGTVQGAL
CCEEEEEECCCCEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
ELADVAYVGGRVLGSAAAMDKDVAKRLLRDAGLPIVPFVTMTAASPVSYDDAARAVGSSE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCC
LFVKPANLGSSVGISKTRDAAEFEAACQLALRFDRKILIERCIAPVREIECAVLEHADGQ
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IKASELGEIVPANSHGFYSYEAKYTDANGAALHVPAQVEPAVAQRIRKMATEVFGALCCE
EEHHHCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
SLARVDFFVRGDEIYVNEVNTLPGFTNISMYPKMWEAAGLPQPALMDELVAHALARHARL
HHHHCEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RELASQR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA