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The map label for this gene is hpcH [H]

Identifier: 146339800

GI number: 146339800

Start: 3007353

End: 3008126

Strand: Reverse

Name: hpcH [H]

Synonym: BRADO2801

Alternate gene names: 146339800

Gene position: 3008126-3007353 (Counterclockwise)

Preceding gene: 146339801

Following gene: 146339799

Centisome position: 40.34

GC content: 67.05

Gene sequence:

>774_bases
ATGTCCGCAACGCCCAAGCCGCTCAACCGCCTCAAACAGGCCTGGCGCGACAAGCGGCCGACCTTCGGCGCCATCGCCAC
CATTCCGAGCATCCAGACGGTGCGCATCATGGCGCAACAGCTCGACTGGATCATTGTCGACCTCGAGCACGGTCCGATCG
ATCTCTCAACCGCGCATGCGATGATCACCGCGACATCAGGCACGCCCTGCGTGCCATTGGTGCGCATCGCCGCCAACGCG
CCGCATCTGGCGAAGGCGCCGATGGACTTGGGGGCGCTCGGCATCAACTTCCCGATGATCTCCAGCCGCGCCGAAGCCGA
GACCGCGGTGCGCAGCGTGCGCTATCCGCCCCGCGGCGACCGGCTCTGGGGCCCGTTCCACGCGCCGTTCCATTGGAACG
TCTCGATGGCCGAGTACATGGCGACCGCCGACGACGACATGATCTGCATGGTCACGATCGAGCATGTCGAGGCGGTCGAG
CGCATCGACGAGATCATGGCGACGCCCGGCATCGACATTGCCGTGATCGGCCCCGGCGACTTGGCGACCTCCATCAACAA
GCGCGGGCTGATCGACGATCCCGAGGTGCAGGCGCTGATGGCCCGCGCCGAGGCCGGCATTCTCAAGTCTGGCGTGCCGA
TCGGCGGCGTGGCGCGCACCGCCGAGCAGGCCAATGCGATGATCGCGCGCGGCTACCTGGCGCTGGCGCTCGGCTTCGAC
TGGTCCTTGTTCCAGCGCGGCATTGCCGCAAGCTTCGAGGGGATCAAGCGCTGA

Upstream 100 bases:

>100_bases
GGTGCCACCATGATCTTCACCTCGAACAAGCCGCATCAGCTCAGCCGCGTGCTGACGCCCTATTTCGGCGGCCGCGTGCC
CGCCTGACGGAGACATGCCG

Downstream 100 bases:

>100_bases
TTCGGCAAGTTGATACCGTCACTTCAGGTTTCGAACGAAAACCAAGATTGGGGGAGAAGCTCATGATGAAAAAGACGATT
TTCGGCCTGTCGCTGGCCGT

Product: putative 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: 2-Hydroxy-3-oxopropanoate; Pyruvate [C]

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSATPKPLNRLKQAWRDKRPTFGAIATIPSIQTVRIMAQQLDWIIVDLEHGPIDLSTAHAMITATSGTPCVPLVRIAANA
PHLAKAPMDLGALGINFPMISSRAEAETAVRSVRYPPRGDRLWGPFHAPFHWNVSMAEYMATADDDMICMVTIEHVEAVE
RIDEIMATPGIDIAVIGPGDLATSINKRGLIDDPEVQALMARAEAGILKSGVPIGGVARTAEQANAMIARGYLALALGFD
WSLFQRGIAASFEGIKR

Sequences:

>Translated_257_residues
MSATPKPLNRLKQAWRDKRPTFGAIATIPSIQTVRIMAQQLDWIIVDLEHGPIDLSTAHAMITATSGTPCVPLVRIAANA
PHLAKAPMDLGALGINFPMISSRAEAETAVRSVRYPPRGDRLWGPFHAPFHWNVSMAEYMATADDDMICMVTIEHVEAVE
RIDEIMATPGIDIAVIGPGDLATSINKRGLIDDPEVQALMARAEAGILKSGVPIGGVARTAEQANAMIARGYLALALGFD
WSLFQRGIAASFEGIKR
>Mature_256_residues
SATPKPLNRLKQAWRDKRPTFGAIATIPSIQTVRIMAQQLDWIIVDLEHGPIDLSTAHAMITATSGTPCVPLVRIAANAP
HLAKAPMDLGALGINFPMISSRAEAETAVRSVRYPPRGDRLWGPFHAPFHWNVSMAEYMATADDDMICMVTIEHVEAVER
IDEIMATPGIDIAVIGPGDLATSINKRGLIDDPEVQALMARAEAGILKSGVPIGGVARTAEQANAMIARGYLALALGFDW
SLFQRGIAASFEGIKR

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=238, Percent_Identity=30.2521008403361, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1788578, Length=203, Percent_Identity=29.5566502463054, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.20 [C]

Molecular weight: Translated: 27729; Mature: 27598

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATPKPLNRLKQAWRDKRPTFGAIATIPSIQTVRIMAQQLDWIIVDLEHGPIDLSTAHA
CCCCCHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCEEEEECCCCCCCEECCEE
MITATSGTPCVPLVRIAANAPHLAKAPMDLGALGINFPMISSRAEAETAVRSVRYPPRGD
EEEECCCCCCHHHHHHHCCCCCHHCCCCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCCC
RLWGPFHAPFHWNVSMAEYMATADDDMICMVTIEHVEAVERIDEIMATPGIDIAVIGPGD
CCCCCCCCCEEECCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCH
LATSINKRGLIDDPEVQALMARAEAGILKSGVPIGGVARTAEQANAMIARGYLALALGFD
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEECCC
WSLFQRGIAASFEGIKR
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SATPKPLNRLKQAWRDKRPTFGAIATIPSIQTVRIMAQQLDWIIVDLEHGPIDLSTAHA
CCCCHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCEEEEECCCCCCCEECCEE
MITATSGTPCVPLVRIAANAPHLAKAPMDLGALGINFPMISSRAEAETAVRSVRYPPRGD
EEEECCCCCCHHHHHHHCCCCCHHCCCCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCCC
RLWGPFHAPFHWNVSMAEYMATADDDMICMVTIEHVEAVERIDEIMATPGIDIAVIGPGD
CCCCCCCCCEEECCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCH
LATSINKRGLIDDPEVQALMARAEAGILKSGVPIGGVARTAEQANAMIARGYLALALGFD
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEECCC
WSLFQRGIAASFEGIKR
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5-Dehydro-4-deoxy-D-glucarate [C]

Specific reaction: 5-Dehydro-4-deoxy-D-glucarate --> 2-Hydroxy-3-oxopropanoate + Pyruvate [C]

General reaction: Carbon-Carbon Lyases; Aldehyde-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA