BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is fadB1 [H]

Identifier: 146339669

GI number: 146339669

Start: 2866840

End: 2867640

Strand: Reverse

Name: fadB1 [H]

Synonym: BRADO2663

Alternate gene names: 146339669

Gene position: 2867640-2866840 (Counterclockwise)

Preceding gene: 146339670

Following gene: 146339668

Centisome position: 38.46

GC content: 65.79

Gene sequence:

>801_bases
TTGATTTTGCAGGGTGGAAACATGAGCGTTTACGAGCACATCATCGTCGAGGGCAAAGGCGCGGTCGGCGTCATCACCTT
GAATCGGCCGAAGATGCTCAACGCATTGTCGTTCGGCGTCTTCCGCGAGATCGCCGCGGCCGTCGACGATCTCGAGGCCG
ACGACGCCATCGGCTGCATCGTCATCACCGGCAGCGAGAAGGCGTTTGCCGCCGGCGCCGACATCAAGGAGATGCAGCCG
AAGACTTTCATCGACATGTTCTCGAGCGACTTCACTGCGATCGGCGGCGACCGCGTCGCGCGCTGTCGCAAGCCGACCAT
CGCCGCCGTCTCGGGCTATGCGCTCGGCGGCGGCTGCGAGCTCGCGATGATGTGCGACTTCATCATCTGCTCCGACACCG
CCAAGTTCGGCCAGCCCGAGATCACGCTCGGCACTATCCCCGGCATCGGCGGCACGCAACGCCTGACGCGCGCGATCGGC
AAGGCCAAGGCGATGGACCTCTGCCTCACCGGCCGCATGATGGACGCGGCCGAAGCCGAGCGCGCCGGCCTGGTCAGCCG
CATCGTGCCCGCCGACAAGTTGATGGAAGAGACGATGGCCGCGGCCGAGAAGATCGCCGCGATGTCGCGGCCCGCCGCCG
CCATGGCCAAGGAGGCCGTCAACCGCGCCTTCGAGACGCAGCTGTCCGAGGGCATGAACGTCGAGCGCAACCTCTTCCAC
GCCACCTTCGCGCTGGAGGACCGCGCAGAGGGGATGGCCGCCTTCATCGAGAAGCGCAAGCCGGTGAATAAGAACCGCTG
A

Upstream 100 bases:

>100_bases
GCTCACCCTGGGCTCCGCCGCGCGGATGTTCATGATCTGACGATCGGCGCCGCCACCCCTACTTGATGCGCAGCCATCCA
GCGGGCAGATTGCCCGCTGT

Downstream 100 bases:

>100_bases
CCGGGACCTGGCGGGGTTCGCCCTGAAGTCGTTCGAACCCCGCCACACGAAGGGTGCGCTCCTCTCCCCGTTCCACGGGA
GGGGCAACCACCATCACCGA

Product: putative enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MILQGGNMSVYEHIIVEGKGAVGVITLNRPKMLNALSFGVFREIAAAVDDLEADDAIGCIVITGSEKAFAAGADIKEMQP
KTFIDMFSSDFTAIGGDRVARCRKPTIAAVSGYALGGGCELAMMCDFIICSDTAKFGQPEITLGTIPGIGGTQRLTRAIG
KAKAMDLCLTGRMMDAAEAERAGLVSRIVPADKLMEETMAAAEKIAAMSRPAAAMAKEAVNRAFETQLSEGMNVERNLFH
ATFALEDRAEGMAAFIEKRKPVNKNR

Sequences:

>Translated_266_residues
MILQGGNMSVYEHIIVEGKGAVGVITLNRPKMLNALSFGVFREIAAAVDDLEADDAIGCIVITGSEKAFAAGADIKEMQP
KTFIDMFSSDFTAIGGDRVARCRKPTIAAVSGYALGGGCELAMMCDFIICSDTAKFGQPEITLGTIPGIGGTQRLTRAIG
KAKAMDLCLTGRMMDAAEAERAGLVSRIVPADKLMEETMAAAEKIAAMSRPAAAMAKEAVNRAFETQLSEGMNVERNLFH
ATFALEDRAEGMAAFIEKRKPVNKNR
>Mature_266_residues
MILQGGNMSVYEHIIVEGKGAVGVITLNRPKMLNALSFGVFREIAAAVDDLEADDAIGCIVITGSEKAFAAGADIKEMQP
KTFIDMFSSDFTAIGGDRVARCRKPTIAAVSGYALGGGCELAMMCDFIICSDTAKFGQPEITLGTIPGIGGTQRLTRAIG
KAKAMDLCLTGRMMDAAEAERAGLVSRIVPADKLMEETMAAAEKIAAMSRPAAAMAKEAVNRAFETQLSEGMNVERNLFH
ATFALEDRAEGMAAFIEKRKPVNKNR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=55.5984555984556, Blast_Score=281, Evalue=4e-76,
Organism=Homo sapiens, GI31542718, Length=244, Percent_Identity=31.1475409836066, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI4502327, Length=262, Percent_Identity=37.7862595419847, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI68989263, Length=187, Percent_Identity=37.4331550802139, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI37594471, Length=285, Percent_Identity=30.8771929824561, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI37594469, Length=271, Percent_Identity=30.9963099630996, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI20127408, Length=209, Percent_Identity=34.4497607655502, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI70995211, Length=270, Percent_Identity=28.8888888888889, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI150378541, Length=242, Percent_Identity=30.9917355371901, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI157694516, Length=231, Percent_Identity=25.5411255411255, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI213417737, Length=231, Percent_Identity=25.5411255411255, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI45643119, Length=260, Percent_Identity=24.2307692307692, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI260275230, Length=260, Percent_Identity=24.2307692307692, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI260274832, Length=260, Percent_Identity=24.2307692307692, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI25453481, Length=269, Percent_Identity=22.6765799256506, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI51538573, Length=269, Percent_Identity=22.6765799256506, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI261878539, Length=93, Percent_Identity=40.8602150537634, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI4757966, Length=219, Percent_Identity=24.6575342465753, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI51538581, Length=219, Percent_Identity=24.6575342465753, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI62530384, Length=257, Percent_Identity=22.9571984435798, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI4757968, Length=261, Percent_Identity=22.2222222222222, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI49355787, Length=261, Percent_Identity=22.2222222222222, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136756, Length=260, Percent_Identity=23.0769230769231, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136753, Length=260, Percent_Identity=23.0769230769231, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221307494, Length=208, Percent_Identity=26.4423076923077, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI157694520, Length=193, Percent_Identity=24.8704663212435, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=42.8015564202335, Blast_Score=197, Evalue=5e-52,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=36.5019011406844, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=33.201581027668, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=31.7647058823529, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=31.9066147859922, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788682, Length=205, Percent_Identity=33.6585365853659, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790281, Length=181, Percent_Identity=31.4917127071823, Blast_Score=95, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=243, Percent_Identity=56.3786008230453, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI25145438, Length=240, Percent_Identity=48.75, Blast_Score=237, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=32, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17558304, Length=227, Percent_Identity=33.4801762114537, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=257, Percent_Identity=31.1284046692607, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17540306, Length=256, Percent_Identity=29.6875, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI25144276, Length=227, Percent_Identity=33.0396475770925, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=268, Percent_Identity=29.8507462686567, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17508953, Length=227, Percent_Identity=33.0396475770925, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=227, Percent_Identity=33.0396475770925, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=225, Percent_Identity=27.1111111111111, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=236, Percent_Identity=29.2372881355932, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=236, Percent_Identity=29.2372881355932, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=253, Percent_Identity=30.4347826086957, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=25.0996015936255, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320241, Length=189, Percent_Identity=30.6878306878307, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20129971, Length=252, Percent_Identity=58.3333333333333, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24653477, Length=252, Percent_Identity=58.3333333333333, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=35.8267716535433, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=29.4573643410853, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19920382, Length=260, Percent_Identity=32.6923076923077, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21357171, Length=262, Percent_Identity=29.0076335877863, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24650670, Length=225, Percent_Identity=29.7777777777778, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24654903, Length=252, Percent_Identity=25.3968253968254, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19921000, Length=229, Percent_Identity=33.6244541484716, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583077, Length=229, Percent_Identity=33.6244541484716, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583079, Length=229, Percent_Identity=33.6244541484716, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571730, Length=179, Percent_Identity=30.7262569832402, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI28571729, Length=179, Percent_Identity=30.7262569832402, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24583165, Length=233, Percent_Identity=25.7510729613734, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921018, Length=175, Percent_Identity=26.8571428571429, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28321; Mature: 28321

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILQGGNMSVYEHIIVEGKGAVGVITLNRPKMLNALSFGVFREIAAAVDDLEADDAIGCI
CEECCCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
VITGSEKAFAAGADIKEMQPKTFIDMFSSDFTAIGGDRVARCRKPTIAAVSGYALGGGCE
EEECCCCCEECCCCHHHCCCHHHHHHHHCCCCCCCCHHHHHHCCCCEEEECCEEECCCCH
LAMMCDFIICSDTAKFGQPEITLGTIPGIGGTQRLTRAIGKAKAMDLCLTGRMMDAAEAE
HHHHHCCCEECCCHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RAGLVSRIVPADKLMEETMAAAEKIAAMSRPAAAMAKEAVNRAFETQLSEGMNVERNLFH
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ATFALEDRAEGMAAFIEKRKPVNKNR
HHEEHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MILQGGNMSVYEHIIVEGKGAVGVITLNRPKMLNALSFGVFREIAAAVDDLEADDAIGCI
CEECCCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
VITGSEKAFAAGADIKEMQPKTFIDMFSSDFTAIGGDRVARCRKPTIAAVSGYALGGGCE
EEECCCCCEECCCCHHHCCCHHHHHHHHCCCCCCCCHHHHHHCCCCEEEECCEEECCCCH
LAMMCDFIICSDTAKFGQPEITLGTIPGIGGTQRLTRAIGKAKAMDLCLTGRMMDAAEAE
HHHHHCCCEECCCHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RAGLVSRIVPADKLMEETMAAAEKIAAMSRPAAAMAKEAVNRAFETQLSEGMNVERNLFH
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ATFALEDRAEGMAAFIEKRKPVNKNR
HHEEHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]