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The map label for this gene is ispH [H]

Identifier: 146339639

GI number: 146339639

Start: 2824586

End: 2825554

Strand: Reverse

Name: ispH [H]

Synonym: BRADO2632

Alternate gene names: 146339639

Gene position: 2825554-2824586 (Counterclockwise)

Preceding gene: 146339641

Following gene: 146339638

Centisome position: 37.89

GC content: 64.6

Gene sequence:

>969_bases
GTGGCTGACGGGCGTCCCTTGCAGAGTCTCTTGCAAAGTCCCTTGCAGGTCTATCTCGCGCAGCCGCGCGGGTTTTGTGC
TGGCGTCGTGCGTGCGATCGAGATCGTTGAGCGCGCGCTGGAGAAGCACGGCCCGCCGGTCTATGTGCGTCACGAGATCG
TTCACAACAAGCACGTCGTGGAGAGCCTGAAGGCCAAAGGCGCCATCTTCGTCGAGGACCTGTCCGAGGTGCCGCCGCGC
GCCGTCACCGTGTTCAGCGCCCACGGCGTTGCCCGCTCGGTCGAGGAAGAGGCTGCGGCTCGAGGCCTGCCGGTGCTCAA
TGCAACCTGCCCGCTGGTCACGAAGGTCCACAACCAGGGCAAGCGCTACATGGCCAAGGGGCGCACCCTGGTGCTGATCG
GCCATGCCGGGCATCCGGAGGTCGAGGGAACCATGGGTCAGGTTCCCGGCCCGGTCCATCTCGTGCAGAACGTGGCCGAT
GTGGCCGCCCTGCCCCTGCCATTGGACACGCCGATGGCCTATATCACCCAGACGACGCTCAGCGTGGACGATACCAGGGA
CATCATTTCGGCGCTCCAAGCCCGCTTTACAGACCTTCAAGGTCCCGATATCCGGGATATCTGCTATGCGACACAGAACC
GGCAATCTGCGGTAAGGGACCTGGGAAAGCTGGTGGACGTCATCCTGGTGGTGGGCGCCGCCAACAGCTCCAACTCGAAC
AGACTGCGCGAGATCGGCACCGAAGCCGGTATCCCGAGCTATCTGATCGCAGACGGGCGCGAGCTGAATCCGGTTTGGCT
GAAAGGGGCCAAGGCCGTCGGCATCACCGCCGGCGCCTCGGCTCCCGAAGTGCTCGTTGACGACGTGATACAGGCCTTGC
GGCGCATCGGACCGGTTGCGGTCTCGGTCGTCCCGGGGCGGGAAGAAAACATCGAATTCCGCTTGCCGGCCGAACTGGTC
GCGGGCTGA

Upstream 100 bases:

>100_bases
GCGCTTCCATGCGCATCCGCGGGCAAATTGGGGCATCCGCTTGGTGTGGATATTGCACTCATTCGCACGCACGGAGGTGG
CAGGTCGAAAGTGAGGCAAC

Downstream 100 bases:

>100_bases
TCGGTCCTCGTTTGGTCCAGAAAGAAGAGACGTAATGGCAATCCCCTTCCATAAGGAACTGCGTATCGGGGCCTATCTGA
TGAAGCAGAAGCTTCTCGGC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MADGRPLQSLLQSPLQVYLAQPRGFCAGVVRAIEIVERALEKHGPPVYVRHEIVHNKHVVESLKAKGAIFVEDLSEVPPR
AVTVFSAHGVARSVEEEAAARGLPVLNATCPLVTKVHNQGKRYMAKGRTLVLIGHAGHPEVEGTMGQVPGPVHLVQNVAD
VAALPLPLDTPMAYITQTTLSVDDTRDIISALQARFTDLQGPDIRDICYATQNRQSAVRDLGKLVDVILVVGAANSSNSN
RLREIGTEAGIPSYLIADGRELNPVWLKGAKAVGITAGASAPEVLVDDVIQALRRIGPVAVSVVPGREENIEFRLPAELV
AG

Sequences:

>Translated_322_residues
MADGRPLQSLLQSPLQVYLAQPRGFCAGVVRAIEIVERALEKHGPPVYVRHEIVHNKHVVESLKAKGAIFVEDLSEVPPR
AVTVFSAHGVARSVEEEAAARGLPVLNATCPLVTKVHNQGKRYMAKGRTLVLIGHAGHPEVEGTMGQVPGPVHLVQNVAD
VAALPLPLDTPMAYITQTTLSVDDTRDIISALQARFTDLQGPDIRDICYATQNRQSAVRDLGKLVDVILVVGAANSSNSN
RLREIGTEAGIPSYLIADGRELNPVWLKGAKAVGITAGASAPEVLVDDVIQALRRIGPVAVSVVPGREENIEFRLPAELV
AG
>Mature_321_residues
ADGRPLQSLLQSPLQVYLAQPRGFCAGVVRAIEIVERALEKHGPPVYVRHEIVHNKHVVESLKAKGAIFVEDLSEVPPRA
VTVFSAHGVARSVEEEAAARGLPVLNATCPLVTKVHNQGKRYMAKGRTLVLIGHAGHPEVEGTMGQVPGPVHLVQNVADV
AALPLPLDTPMAYITQTTLSVDDTRDIISALQARFTDLQGPDIRDICYATQNRQSAVRDLGKLVDVILVVGAANSSNSNR
LREIGTEAGIPSYLIADGRELNPVWLKGAKAVGITAGASAPEVLVDDVIQALRRIGPVAVSVVPGREENIEFRLPAELVA
G

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=308, Percent_Identity=53.5714285714286, Blast_Score=335, Evalue=3e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 34402; Mature: 34271

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADGRPLQSLLQSPLQVYLAQPRGFCAGVVRAIEIVERALEKHGPPVYVRHEIVHNKHVV
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHH
ESLKAKGAIFVEDLSEVPPRAVTVFSAHGVARSVEEEAAARGLPVLNATCPLVTKVHNQG
HHHHHCCCEEEEHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEECCCCHHHHHHHHCC
KRYMAKGRTLVLIGHAGHPEVEGTMGQVPGPVHLVQNVADVAALPLPLDTPMAYITQTTL
HHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHC
SVDDTRDIISALQARFTDLQGPDIRDICYATQNRQSAVRDLGKLVDVILVVGAANSSNSN
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCCCH
RLREIGTEAGIPSYLIADGRELNPVWLKGAKAVGITAGASAPEVLVDDVIQALRRIGPVA
HHHHHHHHCCCCCEEECCCCCCCHHEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEE
VSVVPGREENIEFRLPAELVAG
EEEECCCCCCEEEECCHHHHCC
>Mature Secondary Structure 
ADGRPLQSLLQSPLQVYLAQPRGFCAGVVRAIEIVERALEKHGPPVYVRHEIVHNKHVV
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHH
ESLKAKGAIFVEDLSEVPPRAVTVFSAHGVARSVEEEAAARGLPVLNATCPLVTKVHNQG
HHHHHCCCEEEEHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEECCCCHHHHHHHHCC
KRYMAKGRTLVLIGHAGHPEVEGTMGQVPGPVHLVQNVADVAALPLPLDTPMAYITQTTL
HHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHC
SVDDTRDIISALQARFTDLQGPDIRDICYATQNRQSAVRDLGKLVDVILVVGAANSSNSN
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCCCH
RLREIGTEAGIPSYLIADGRELNPVWLKGAKAVGITAGASAPEVLVDDVIQALRRIGPVA
HHHHHHHHCCCCCEEECCCCCCCHHEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEE
VSVVPGREENIEFRLPAELVAG
EEEECCCCCCEEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]