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The map label for this gene is gsiB [H]

Identifier: 146339515

GI number: 146339515

Start: 2677305

End: 2678777

Strand: Reverse

Name: gsiB [H]

Synonym: BRADO2505

Alternate gene names: 146339515

Gene position: 2678777-2677305 (Counterclockwise)

Preceding gene: 146339516

Following gene: 146339514

Centisome position: 35.92

GC content: 64.63

Gene sequence:

>1473_bases
ATGGCCTCTTGCGGGACAGCCGCCCAGGCGCAAACTACGCTGCGCATTGGGCTCGCCGAGGACGCCGACATTCTCGATCC
GACGATGGCGCGGACCTATGTCGGCCGCATCGTGTTCGCCGCCGTCTGCGACAAGCTGTTCGACATCGACGAGAAGCTGA
ACATCGTGCCGCAGCTCGCGCTGTCGCACGAGACCTCCGCCGACGGCAAGGAAGTCACGATCAGGCTGCGGCCCGGCGTC
AAATTTCATGACGGCGAGCCGTTCGATGCCGAGGCGGCCAAGTTCTCGCTCGATCGTCACCTCTCGATGCCCGGCTCGTT
CCGCAAGCCGGAGATCGCCTCGCTCGACCATGTCGATGTCGTCGATCCCCTGACGATCAAGCTGGTCTTGAAGACGCCGT
TCTCGCCGCTGCTCGCCCAGCTCACCGACCGCGCCGGCATGATGGTGTCGCCGAAGGCCGCCAAAGAGGCCGGCGACAAG
TTCGGCCTGCGCCCGGTCTGCGCCGGCCCCTACAAATTCGTCGAGCGCGTCCAGCAGGACCGCATCGTGTTCGAGAAGTT
CGCCGACTACTGGAACAAGGACAACGTCTTCATCGACAAGATCGTCTATCAGCCGATCGTCGACGCCACGGTGCGGCTGG
CGAACCTCAAGTCCGGCAGCCTCGACATGATCGAGCGGCTGCTCGCCACCGACATCAAGGACGTCACCAGCGACAAGCGC
CTGAAGCTCTCGACCGCGACCAGCCTCGGCTATCGCGGTCTGACGCTCAATATCGGCAAGGACAAGACCAAGGGCCCGCT
CAGCCAGTCGGCGAAGGTCCGCCAGGCCTTGGATCTGTCGATCGATCGCGAGGCGCTCAACCAGGTGGTCTTCAACGGCG
AGTTCAAGCCTGGCAATCAATTCGTCAACCCCGAGAACCCCTACTACCAGTCCGCCTTCCCGGTGCGCGGCCGTGACGTC
GAGAAGGCCAAGGCGCTCTTGAAGGAAGCCGGCGTGGCGCCGCCCGTCACCGTCGACTTCATGGTGCCGAAGGAGAGCGA
GAGCGAAGCCGCCGCGCAGGTGCTGCAGGCCATGGCGGCGGAGGCCGGCTTCGACCTGAAGATCCGCATCACCGAGTTCG
CGACCTCGCTCAAACAGGCCGAGGCCGGCGAGTACCAGGCCTACATGCTGGAATGGAGTGGCCGCAGCGATCCCGACGGC
AACACCTACAACCACCTGCATTCCGGCGCGGCGCTCAACTACAGCGCCTGGAGCAACGCCGCCGCCGACAAGGCGCTGGA
GGACGCGCGGCTCGTGAGCGACATCGCCAAGCGCAAGGCGATCTACGAGAGGCTGGTCAAGGAGCAGCAGGCCGACGAGC
CGATCCTGTATCTCTATCACCGCAAGTTCCTGTTCGCGCAGACCACGAAGCTGGAAGGCTTCAAGCCCATGCCGGACGGC
CTGCTGCGCGTGGTCGGGCTGAAGCTGAAGTGA

Upstream 100 bases:

>100_bases
GGGGTTGGTACTGATAGTGCAACAATGCAGGCTGCATGTTCGTAGGGGATTTCGTATGCTGAGGAAGAACTGGTGCTTGG
CCGCAATCGCGGCCGTCGTC

Downstream 100 bases:

>100_bases
GGGCTGAGTTGTCAAGAGAAGCTATCACTCGGTTGTTCGTCATGCCCGGGCTTGACCACGCCTTCGTTTCCAAAGGCCGC
AAAGACGTGGATGGCCGGGT

Product: putative ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MASCGTAAQAQTTLRIGLAEDADILDPTMARTYVGRIVFAAVCDKLFDIDEKLNIVPQLALSHETSADGKEVTIRLRPGV
KFHDGEPFDAEAAKFSLDRHLSMPGSFRKPEIASLDHVDVVDPLTIKLVLKTPFSPLLAQLTDRAGMMVSPKAAKEAGDK
FGLRPVCAGPYKFVERVQQDRIVFEKFADYWNKDNVFIDKIVYQPIVDATVRLANLKSGSLDMIERLLATDIKDVTSDKR
LKLSTATSLGYRGLTLNIGKDKTKGPLSQSAKVRQALDLSIDREALNQVVFNGEFKPGNQFVNPENPYYQSAFPVRGRDV
EKAKALLKEAGVAPPVTVDFMVPKESESEAAAQVLQAMAAEAGFDLKIRITEFATSLKQAEAGEYQAYMLEWSGRSDPDG
NTYNHLHSGAALNYSAWSNAAADKALEDARLVSDIAKRKAIYERLVKEQQADEPILYLYHRKFLFAQTTKLEGFKPMPDG
LLRVVGLKLK

Sequences:

>Translated_490_residues
MASCGTAAQAQTTLRIGLAEDADILDPTMARTYVGRIVFAAVCDKLFDIDEKLNIVPQLALSHETSADGKEVTIRLRPGV
KFHDGEPFDAEAAKFSLDRHLSMPGSFRKPEIASLDHVDVVDPLTIKLVLKTPFSPLLAQLTDRAGMMVSPKAAKEAGDK
FGLRPVCAGPYKFVERVQQDRIVFEKFADYWNKDNVFIDKIVYQPIVDATVRLANLKSGSLDMIERLLATDIKDVTSDKR
LKLSTATSLGYRGLTLNIGKDKTKGPLSQSAKVRQALDLSIDREALNQVVFNGEFKPGNQFVNPENPYYQSAFPVRGRDV
EKAKALLKEAGVAPPVTVDFMVPKESESEAAAQVLQAMAAEAGFDLKIRITEFATSLKQAEAGEYQAYMLEWSGRSDPDG
NTYNHLHSGAALNYSAWSNAAADKALEDARLVSDIAKRKAIYERLVKEQQADEPILYLYHRKFLFAQTTKLEGFKPMPDG
LLRVVGLKLK
>Mature_489_residues
ASCGTAAQAQTTLRIGLAEDADILDPTMARTYVGRIVFAAVCDKLFDIDEKLNIVPQLALSHETSADGKEVTIRLRPGVK
FHDGEPFDAEAAKFSLDRHLSMPGSFRKPEIASLDHVDVVDPLTIKLVLKTPFSPLLAQLTDRAGMMVSPKAAKEAGDKF
GLRPVCAGPYKFVERVQQDRIVFEKFADYWNKDNVFIDKIVYQPIVDATVRLANLKSGSLDMIERLLATDIKDVTSDKRL
KLSTATSLGYRGLTLNIGKDKTKGPLSQSAKVRQALDLSIDREALNQVVFNGEFKPGNQFVNPENPYYQSAFPVRGRDVE
KAKALLKEAGVAPPVTVDFMVPKESESEAAAQVLQAMAAEAGFDLKIRITEFATSLKQAEAGEYQAYMLEWSGRSDPDGN
TYNHLHSGAALNYSAWSNAAADKALEDARLVSDIAKRKAIYERLVKEQQADEPILYLYHRKFLFAQTTKLEGFKPMPDGL
LRVVGLKLK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=493, Percent_Identity=28.1947261663286, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789966, Length=461, Percent_Identity=29.7180043383948, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1789887, Length=449, Percent_Identity=24.9443207126949, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787762, Length=501, Percent_Identity=24.9500998003992, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081878, Length=491, Percent_Identity=24.847250509165, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1787495, Length=527, Percent_Identity=22.9601518026565, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789397, Length=504, Percent_Identity=22.4206349206349, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787551, Length=481, Percent_Identity=22.037422037422, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87082063, Length=278, Percent_Identity=23.7410071942446, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 54146; Mature: 54014

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASCGTAAQAQTTLRIGLAEDADILDPTMARTYVGRIVFAAVCDKLFDIDEKLNIVPQLA
CCCCCCCHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LSHETSADGKEVTIRLRPGVKFHDGEPFDAEAAKFSLDRHLSMPGSFRKPEIASLDHVDV
HCCCCCCCCCEEEEEECCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
VDPLTIKLVLKTPFSPLLAQLTDRAGMMVSPKAAKEAGDKFGLRPVCAGPYKFVERVQQD
CCCEEEEEEECCCCHHHHHHHHHCCCCEECCHHHHHCCHHCCCCCCCCCHHHHHHHHHHH
RIVFEKFADYWNKDNVFIDKIVYQPIVDATVRLANLKSGSLDMIERLLATDIKDVTSDKR
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCE
LKLSTATSLGYRGLTLNIGKDKTKGPLSQSAKVRQALDLSIDREALNQVVFNGEFKPGNQ
EEEEEHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCC
FVNPENPYYQSAFPVRGRDVEKAKALLKEAGVAPPVTVDFMVPKESESEAAAQVLQAMAA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
EAGFDLKIRITEFATSLKQAEAGEYQAYMLEWSGRSDPDGNTYNHLHSGAALNYSAWSNA
HCCCCEEEEHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHCCCCEEECHHHCCH
AADKALEDARLVSDIAKRKAIYERLVKEQQADEPILYLYHRKFLFAQTTKLEGFKPMPDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHEECCCCCCCCCCHHH
LLRVVGLKLK
HHHHHCCCCC
>Mature Secondary Structure 
ASCGTAAQAQTTLRIGLAEDADILDPTMARTYVGRIVFAAVCDKLFDIDEKLNIVPQLA
CCCCCCHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LSHETSADGKEVTIRLRPGVKFHDGEPFDAEAAKFSLDRHLSMPGSFRKPEIASLDHVDV
HCCCCCCCCCEEEEEECCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
VDPLTIKLVLKTPFSPLLAQLTDRAGMMVSPKAAKEAGDKFGLRPVCAGPYKFVERVQQD
CCCEEEEEEECCCCHHHHHHHHHCCCCEECCHHHHHCCHHCCCCCCCCCHHHHHHHHHHH
RIVFEKFADYWNKDNVFIDKIVYQPIVDATVRLANLKSGSLDMIERLLATDIKDVTSDKR
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCE
LKLSTATSLGYRGLTLNIGKDKTKGPLSQSAKVRQALDLSIDREALNQVVFNGEFKPGNQ
EEEEEHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCC
FVNPENPYYQSAFPVRGRDVEKAKALLKEAGVAPPVTVDFMVPKESESEAAAQVLQAMAA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
EAGFDLKIRITEFATSLKQAEAGEYQAYMLEWSGRSDPDGNTYNHLHSGAALNYSAWSNA
HCCCCEEEEHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHCCCCEEECHHHCCH
AADKALEDARLVSDIAKRKAIYERLVKEQQADEPILYLYHRKFLFAQTTKLEGFKPMPDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHEECCCCCCCCCCHHH
LLRVVGLKLK
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA