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The map label for this gene is fixL [H]

Identifier: 146339449

GI number: 146339449

Start: 2595203

End: 2596714

Strand: Reverse

Name: fixL [H]

Synonym: BRADO2435

Alternate gene names: 146339449

Gene position: 2596714-2595203 (Counterclockwise)

Preceding gene: 146339460

Following gene: 146339448

Centisome position: 34.82

GC content: 66.93

Gene sequence:

>1512_bases
GTGCGCGGCGTCACTCAACCTTTTCCGGATGCGTCCGTCCGTCCCGAAGATGCCCATGCGTTGGGGTTCGGCGCGTGGGA
TCTCGACTTCGCCACCGGCGACTTCTACTGGTCCGCGACCACGCGCAAGCTGTTCGGGATCCCGCAAGACCAGCCGGTGA
CTTACGGGGCCTTCCTGGCCCTGCTCGGGGCCCAGGATCGTGCCCCGGTCGAAGCGGCGATCGAGCATGCGCGCCACCAG
GGCACCTGGTTCGACATGTCGTTCAGGGTGCCGAACGGCGGACACTGGATTCGCGTGCTTGCCGGCGTGGTGCCCGGCGA
CGACGGCCAGCCGCGTCATCTGCGCGGCATCGCGCTGGACATCGACCAGGAGAAGCGGCTCGAGGAAGCGTTGCGGCTGC
GCGAGCAGCACTTCCGCTCGATCCTCGACACCGTGCCCGACGCGATGATCGTGATCGACGGCGGCGGCATCATCCAGTTC
TTCAGCGCCGCGGCCGAGCGAATGTTCGGCTACACCGAGGAGGAAGCGGTCGGCCTCGACGTCAGCGAGCTGATGCCGGA
GCCCGACCGCTCGCGCCATGCCGGCTATCTCACCCGTTATCACGGCACCCGCGAGCGCCACATCATCGGCATCGGCCGTA
TCGTGACCGGGCTGCGGCGCGACGGCACGACCTTCCCGATGCATCTGTCGATCGGCGAGATGCAGTCCGGCGGCAAGCCC
TATTTCACCGGCTTCGCCCATGACCTCACCGAGCATCAGCAGACGCAGGCGCGGCTGCGCGAGCTGCAGTCCGAGCTGGT
CCATGTCTCGCGGCTCAGCGCGATGGGCGAGATGGCCTCGGCGCTGGCGCACGAGCTCAACCAGCCGCTCGCCGCCATCA
GCAACTACATGAAGGGCTCGCGCCGGCTGCTCGCCGGCAGCAGCGATCCGAACACGGGGAAGATCGAAAGCGCGATGGAC
CGCGCCGCCGAGCAGGCGATCCGCGCCGGCCAGATCATCCGCCGCCTGCGCGACTTCGTCTCGCGCGGTGAATCGGAAAA
GCGCGTCGAAAGCCTGTCGAAGCTGATCGAGGAAGCCGGAGCGCTCGGCCTTGCCGGCGCGCGCGAGCAGAACGTCCAGT
TGCGCTTTCAGCTCAATCCGGATGCCGACCTGGTGCTGGCCGACCGCGTGCAGATCCAGCAGGTGCTGGTCAATCTGTTT
CGCAACGCGCTCGAGGCGATGTCGCATTCCGCGCGGCGCGAGCTCGCGGCGACCAATCGCCGGGTCGGCGACGACTTGAT
CGAGGTCGCCGTCTCGGATACCGGGACGGGGTTCCACGACGACGTCATGCCCAACCTTTTCAAGACCTTCTTCACCACCA
AGGATACCGGAATGGGAGTCGGCCTGTCGATCAGCCGTTCCATCATCGAGGCCCATGGCGGGCGCATGTGGGCCGAAAAC
AACGAGGCAGGCGGCGCGACATTCCGCTTCACCCTGCCCGCCGCTCCAACCAAGGTTGAGCATGATGGCTGA

Upstream 100 bases:

>100_bases
CTCGGCAAGTGGGCTTGCGGCAGAACGGGTTTTGGCAGCACGGGTTTTCGGCAAAACGGCTTCTCTCTGAACCACGGCGA
ATCGATTCGAAAGTCTTGAA

Downstream 100 bases:

>100_bases
ACGGGGAAGGGTCTATGTGATCGACGACGACGAGGCGATGCGGGATTCGCTCAACTTCCTGCTCGATTCCTCGGGCTTCG
ACGTCACGCTGTTCGAGAAT

Product: sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MRGVTQPFPDASVRPEDAHALGFGAWDLDFATGDFYWSATTRKLFGIPQDQPVTYGAFLALLGAQDRAPVEAAIEHARHQ
GTWFDMSFRVPNGGHWIRVLAGVVPGDDGQPRHLRGIALDIDQEKRLEEALRLREQHFRSILDTVPDAMIVIDGGGIIQF
FSAAAERMFGYTEEEAVGLDVSELMPEPDRSRHAGYLTRYHGTRERHIIGIGRIVTGLRRDGTTFPMHLSIGEMQSGGKP
YFTGFAHDLTEHQQTQARLRELQSELVHVSRLSAMGEMASALAHELNQPLAAISNYMKGSRRLLAGSSDPNTGKIESAMD
RAAEQAIRAGQIIRRLRDFVSRGESEKRVESLSKLIEEAGALGLAGAREQNVQLRFQLNPDADLVLADRVQIQQVLVNLF
RNALEAMSHSARRELAATNRRVGDDLIEVAVSDTGTGFHDDVMPNLFKTFFTTKDTGMGVGLSISRSIIEAHGGRMWAEN
NEAGGATFRFTLPAAPTKVEHDG

Sequences:

>Translated_503_residues
MRGVTQPFPDASVRPEDAHALGFGAWDLDFATGDFYWSATTRKLFGIPQDQPVTYGAFLALLGAQDRAPVEAAIEHARHQ
GTWFDMSFRVPNGGHWIRVLAGVVPGDDGQPRHLRGIALDIDQEKRLEEALRLREQHFRSILDTVPDAMIVIDGGGIIQF
FSAAAERMFGYTEEEAVGLDVSELMPEPDRSRHAGYLTRYHGTRERHIIGIGRIVTGLRRDGTTFPMHLSIGEMQSGGKP
YFTGFAHDLTEHQQTQARLRELQSELVHVSRLSAMGEMASALAHELNQPLAAISNYMKGSRRLLAGSSDPNTGKIESAMD
RAAEQAIRAGQIIRRLRDFVSRGESEKRVESLSKLIEEAGALGLAGAREQNVQLRFQLNPDADLVLADRVQIQQVLVNLF
RNALEAMSHSARRELAATNRRVGDDLIEVAVSDTGTGFHDDVMPNLFKTFFTTKDTGMGVGLSISRSIIEAHGGRMWAEN
NEAGGATFRFTLPAAPTKVEHDG
>Mature_503_residues
MRGVTQPFPDASVRPEDAHALGFGAWDLDFATGDFYWSATTRKLFGIPQDQPVTYGAFLALLGAQDRAPVEAAIEHARHQ
GTWFDMSFRVPNGGHWIRVLAGVVPGDDGQPRHLRGIALDIDQEKRLEEALRLREQHFRSILDTVPDAMIVIDGGGIIQF
FSAAAERMFGYTEEEAVGLDVSELMPEPDRSRHAGYLTRYHGTRERHIIGIGRIVTGLRRDGTTFPMHLSIGEMQSGGKP
YFTGFAHDLTEHQQTQARLRELQSELVHVSRLSAMGEMASALAHELNQPLAAISNYMKGSRRLLAGSSDPNTGKIESAMD
RAAEQAIRAGQIIRRLRDFVSRGESEKRVESLSKLIEEAGALGLAGAREQNVQLRFQLNPDADLVLADRVQIQQVLVNLF
RNALEAMSHSARRELAATNRRVGDDLIEVAVSDTGTGFHDDVMPNLFKTFFTTKDTGMGVGLSISRSIIEAHGGRMWAEN
NEAGGATFRFTLPAAPTKVEHDG

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=398, Percent_Identity=29.6482412060301, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1790436, Length=242, Percent_Identity=29.7520661157025, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788381, Length=160, Percent_Identity=30, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI48994928, Length=404, Percent_Identity=24.2574257425743, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788393, Length=148, Percent_Identity=30.4054054054054, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790300, Length=358, Percent_Identity=24.0223463687151, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1786783, Length=230, Percent_Identity=26.0869565217391, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55443; Mature: 55443

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGVTQPFPDASVRPEDAHALGFGAWDLDFATGDFYWSATTRKLFGIPQDQPVTYGAFLA
CCCCCCCCCCCCCCCCCCCEEEECCEEECCCCCCEEEECCCHHHHCCCCCCCCHHHHHHH
LLGAQDRAPVEAAIEHARHQGTWFDMSFRVPNGGHWIRVLAGVVPGDDGQPRHLRGIALD
HHCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHEECCCCCCCCCCCEEEEEEEC
IDQEKRLEEALRLREQHFRSILDTVPDAMIVIDGGGIIQFFSAAAERMFGYTEEEAVGLD
CCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCHHHCCCC
VSELMPEPDRSRHAGYLTRYHGTRERHIIGIGRIVTGLRRDGTTFPMHLSIGEMQSGGKP
HHHHCCCCCCCCCCCCCEECCCCCCCEEEEHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
YFTGFAHDLTEHQQTQARLRELQSELVHVSRLSAMGEMASALAHELNQPLAAISNYMKGS
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
RRLLAGSSDPNTGKIESAMDRAAEQAIRAGQIIRRLRDFVSRGESEKRVESLSKLIEEAG
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
ALGLAGAREQNVQLRFQLNPDADLVLADRVQIQQVLVNLFRNALEAMSHSARRELAATNR
CCCCCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVGDDLIEVAVSDTGTGFHDDVMPNLFKTFFTTKDTGMGVGLSISRSIIEAHGGRMWAEN
HCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEECCC
NEAGGATFRFTLPAAPTKVEHDG
CCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MRGVTQPFPDASVRPEDAHALGFGAWDLDFATGDFYWSATTRKLFGIPQDQPVTYGAFLA
CCCCCCCCCCCCCCCCCCCEEEECCEEECCCCCCEEEECCCHHHHCCCCCCCCHHHHHHH
LLGAQDRAPVEAAIEHARHQGTWFDMSFRVPNGGHWIRVLAGVVPGDDGQPRHLRGIALD
HHCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHEECCCCCCCCCCCEEEEEEEC
IDQEKRLEEALRLREQHFRSILDTVPDAMIVIDGGGIIQFFSAAAERMFGYTEEEAVGLD
CCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCHHHCCCC
VSELMPEPDRSRHAGYLTRYHGTRERHIIGIGRIVTGLRRDGTTFPMHLSIGEMQSGGKP
HHHHCCCCCCCCCCCCCEECCCCCCCEEEEHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
YFTGFAHDLTEHQQTQARLRELQSELVHVSRLSAMGEMASALAHELNQPLAAISNYMKGS
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
RRLLAGSSDPNTGKIESAMDRAAEQAIRAGQIIRRLRDFVSRGESEKRVESLSKLIEEAG
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
ALGLAGAREQNVQLRFQLNPDADLVLADRVQIQQVLVNLFRNALEAMSHSARRELAATNR
CCCCCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVGDDLIEVAVSDTGTGFHDDVMPNLFKTFFTTKDTGMGVGLSISRSIIEAHGGRMWAEN
HCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEECCC
NEAGGATFRFTLPAAPTKVEHDG
CCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]