Click here to switch to the map view.

The map label for this gene is pcaK [H]

Identifier: 146339355

GI number: 146339355

Start: 2489108

End: 2490484

Strand: Direct

Name: pcaK [H]

Synonym: BRADO2336

Alternate gene names: 146339355

Gene position: 2489108-2490484 (Clockwise)

Preceding gene: 146339354

Following gene: 146339356

Centisome position: 33.38

GC content: 67.9

Gene sequence:

>1377_bases
ATGCCCACGACCGCCCGTGTCGACGTCCAGGCCTTTCTCGACGACCACCCGTTTTCCGGTTTCCAGTGGCTGATCTTCGC
GCTCGGCTTCGTCATCGTGCTGCTGGACGGCTTCGACACCGCTGCCATCGGCTTCATCGCGCCGTCGCTGCTCAAGGAGT
GGGGCATCGAGAAGTCGGCGCTCGGGCCGGTGCTGAGCGCCGCGCTGTTCGGCCTGGCCGCCGGCGCGCTGTTCGCCGGT
CCGGTGGCCGACCGTCTGGGCCGCAAGCTGGTGCTGGTCGGCTCGGTGCTGATCTTCGGCGTGGCCTGTCTCGCCTCGGC
GTTCGCCACCAACCTCACCGAGCTCACCGTGCTCCGCTGCGTCACCGGCGTCGGCCTCGGCGCGGCGATGCCCAACGCCG
TGACCCTGCTCAGCGAGTACTCGCCGAGCCGGCTGCGCGCCACGATGACCAATCTGATGTTCTGCGGCTTCCCGCTGGGC
GCGGCCTGCGGCGGCTTCCTCGCGGCCTGGATGATCCCGCAATGGGGCTGGCGCAGCGTGCTGGTGCTCGGCGGCGTCAC
GCCGCTGGTGCTCACGGTGTTGCTGATCGCGATCCTGCCTGAATCCGTCCACTATTTGCTGTCGCGCGGCGCTTCGGTCG
AACGCGTCCGCAAGGTGATGCGCCGGATCTCGGCCAAGGCCGCCGATGTCGGCCAGTTCGTGATGCTGGAGCCGGAGCAC
GCGGAGACCACCCGGCGCGTGAACGCGACCGGTATCGGCATGGTGCTGTCGCCGGTGCTGCGGATCGGCTCGCTGATGCT
GTGGGTCACCTATTTCATGGGCCTGGTGATCTTCTATTCGCTGATCAACTGGATGCCGATCCTGTTCAAGGATGTCGGAC
TCAACCCGCAGACCGCGACCCTGATCTCGGCGCTGTTCCCGCTCGGTGGCGTCGGCGCGGTGTTCTTCGGGTTCCTGATG
GACCGCTTCAATGCCAACCGGATCGTGGCGGTCGGCTATGCGCTGACGGCGGTGACGATCTTCCTGATCGGGCAGGCGGC
CGGCAACATGGGCTTCCTGGTGGTGATCGTGTTCGTCGCCGGCGTCGTCATGAACACGGCGCAGTCGTCGCTGCCGGCGC
TGGCGGCGTCGTTTTACCCGACCCAGGGCCGCGCCACCGGCGTCGCCTGGATGATGGGCATCGGCCGCTTCGGCGGTATC
GCCGGCTCCTTCCTGGTCGCCGAACTGACCGCGCGGCACCTGACCTTCAGCGAGATTTTCACCGTGATCGCAGTTCCCGG
CTTGATCGCGGCGCTCGCCCTTGTGGTAAAGCAGGTCGTGGGACCGGCTGGCCGCGCCAGCGGCAAGGATGCGGGCGCCC
CCGTGGTCGCGCATTGA

Upstream 100 bases:

>100_bases
GCCGAAGCTGCAGGACCCATCCGCGCCACGAACGCGCGCCTGCGGACCTCGGTCAACGCGCCAGAACAGCACCCAAGAGC
ACCCAAGGGAAGCAGAAGCG

Downstream 100 bases:

>100_bases
GCGGTTCGCAAGAAAATTTGAGGGAGAGACTGACGTGATCATCGACGTGCACGGCCATTACACGACGGCCCCGAAGGCGC
TGGAAGAGTGGCGCAACCGG

Product: MFS superfamily protocatechuate transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MPTTARVDVQAFLDDHPFSGFQWLIFALGFVIVLLDGFDTAAIGFIAPSLLKEWGIEKSALGPVLSAALFGLAAGALFAG
PVADRLGRKLVLVGSVLIFGVACLASAFATNLTELTVLRCVTGVGLGAAMPNAVTLLSEYSPSRLRATMTNLMFCGFPLG
AACGGFLAAWMIPQWGWRSVLVLGGVTPLVLTVLLIAILPESVHYLLSRGASVERVRKVMRRISAKAADVGQFVMLEPEH
AETTRRVNATGIGMVLSPVLRIGSLMLWVTYFMGLVIFYSLINWMPILFKDVGLNPQTATLISALFPLGGVGAVFFGFLM
DRFNANRIVAVGYALTAVTIFLIGQAAGNMGFLVVIVFVAGVVMNTAQSSLPALAASFYPTQGRATGVAWMMGIGRFGGI
AGSFLVAELTARHLTFSEIFTVIAVPGLIAALALVVKQVVGPAGRASGKDAGAPVVAH

Sequences:

>Translated_458_residues
MPTTARVDVQAFLDDHPFSGFQWLIFALGFVIVLLDGFDTAAIGFIAPSLLKEWGIEKSALGPVLSAALFGLAAGALFAG
PVADRLGRKLVLVGSVLIFGVACLASAFATNLTELTVLRCVTGVGLGAAMPNAVTLLSEYSPSRLRATMTNLMFCGFPLG
AACGGFLAAWMIPQWGWRSVLVLGGVTPLVLTVLLIAILPESVHYLLSRGASVERVRKVMRRISAKAADVGQFVMLEPEH
AETTRRVNATGIGMVLSPVLRIGSLMLWVTYFMGLVIFYSLINWMPILFKDVGLNPQTATLISALFPLGGVGAVFFGFLM
DRFNANRIVAVGYALTAVTIFLIGQAAGNMGFLVVIVFVAGVVMNTAQSSLPALAASFYPTQGRATGVAWMMGIGRFGGI
AGSFLVAELTARHLTFSEIFTVIAVPGLIAALALVVKQVVGPAGRASGKDAGAPVVAH
>Mature_457_residues
PTTARVDVQAFLDDHPFSGFQWLIFALGFVIVLLDGFDTAAIGFIAPSLLKEWGIEKSALGPVLSAALFGLAAGALFAGP
VADRLGRKLVLVGSVLIFGVACLASAFATNLTELTVLRCVTGVGLGAAMPNAVTLLSEYSPSRLRATMTNLMFCGFPLGA
ACGGFLAAWMIPQWGWRSVLVLGGVTPLVLTVLLIAILPESVHYLLSRGASVERVRKVMRRISAKAADVGQFVMLEPEHA
ETTRRVNATGIGMVLSPVLRIGSLMLWVTYFMGLVIFYSLINWMPILFKDVGLNPQTATLISALFPLGGVGAVFFGFLMD
RFNANRIVAVGYALTAVTIFLIGQAAGNMGFLVVIVFVAGVVMNTAQSSLPALAASFYPTQGRATGVAWMMGIGRFGGIA
GSFLVAELTARHLTFSEIFTVIAVPGLIAALALVVKQVVGPAGRASGKDAGAPVVAH

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=461, Percent_Identity=24.5119305856833, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI4506999, Length=386, Percent_Identity=27.2020725388601, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI302148518, Length=357, Percent_Identity=25.7703081232493, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI166064021, Length=363, Percent_Identity=25.068870523416, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI7662270, Length=217, Percent_Identity=24.8847926267281, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI23510410, Length=215, Percent_Identity=29.3023255813954, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI8923870, Length=392, Percent_Identity=24.7448979591837, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI216548223, Length=410, Percent_Identity=22.6829268292683, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI87081723, Length=392, Percent_Identity=34.9489795918367, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI87082231, Length=442, Percent_Identity=25.7918552036199, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788074, Length=361, Percent_Identity=26.8698060941828, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786229, Length=233, Percent_Identity=27.0386266094421, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082404, Length=408, Percent_Identity=23.5294117647059, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87082159, Length=395, Percent_Identity=24.5569620253165, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789207, Length=314, Percent_Identity=27.7070063694268, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788068, Length=453, Percent_Identity=24.2825607064018, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790463, Length=386, Percent_Identity=24.3523316062176, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787880, Length=160, Percent_Identity=29.375, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=428, Percent_Identity=24.7663551401869, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71980582, Length=361, Percent_Identity=22.7146814404432, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71980584, Length=339, Percent_Identity=22.1238938053097, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17565978, Length=179, Percent_Identity=28.4916201117318, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320595, Length=208, Percent_Identity=28.8461538461538, Blast_Score=63, Evalue=1e-10,
Organism=Drosophila melanogaster, GI19922874, Length=446, Percent_Identity=24.6636771300448, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24640198, Length=462, Percent_Identity=24.6753246753247, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640196, Length=462, Percent_Identity=24.6753246753247, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640200, Length=462, Percent_Identity=24.6753246753247, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48138; Mature: 48007

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PTTARVDVQAFLDDHPFSGFQWLIFALGFVIVLLDGFDTAAIGFIAPSLLKEWGIEKSA
CCCCEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHH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HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]