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The map label for this gene is dctA

Identifier: 146339322

GI number: 146339322

Start: 2450843

End: 2452162

Strand: Direct

Name: dctA

Synonym: BRADO2301

Alternate gene names: 146339322

Gene position: 2450843-2452162 (Clockwise)

Preceding gene: 146339321

Following gene: 146339323

Centisome position: 32.87

GC content: 64.24

Gene sequence:

>1320_bases
ATGTCTGCAACCACACCAGCAGCCGCGGCCCCGAAGAAGCCGTTCTACCGCGTCCTCTATATCCAGGTTCTGGTCGCGAT
CGTGCTCGGCGTGATCGTCGGCTGGCTATGGCCCGAGATCGGCAAGAACGACTGGATCAAGGCGCTCGGCGACGGCTTCG
TCAAGCTGATCAAGATGGTGATCGCGCCGGTCATTTTCTGTACCGTCGTCTCGGGCATCGCCCATGTCTCCGAGGTGAAG
AAGGTCGGCCGAGTCGCCATCAAGGCGCTGATCTATTTCGAGATCGTCTCGACCTTCGCGCTTGCGCTCGGGCTGATCGT
CGCGAATGTCATTCAGCCCGGTGCCGGCTTCCAGGGCCAGACCAACGCCGCCGCGGTTGCCAACTACGCCAAGCAGGCGA
GCGAGATGAAGTCGGTCGACTTCGTGCTCCACATCATTCCGGACACTGTGGTCGGCGCGTTTGCGCAAGGCGAGATACTG
CAGGTGCTGCTGTTCTCGATCCTTTTCGGCATCGCGCTGATGAGCCTCGGCGAGCGCGGCCACACCATGCGCGCCTTCGT
CGACGACGCGGCGCATGCGATCTTCGGCGTCATCGCCATCGTCGTGAAGGCCGCCCCGATCGGCGCGTTCGGCGCGATGG
CTTTCACCATCGGCCGCTATGGCCCGCAGGCGCTCGGCAACCTCGCCGGCCTGATCGCGACCTTCTATCTCACCGCGCTG
GCCTTCGTCATCATCGTGCTCGGCATCATCGCCCGCATCGCCGGCTTCTCGATCTTCAAGTTCCTGACATACATCAAGGA
CGAACTGTTGATCGTGCTCGGCACCTCGTCGTCGGAGAGCGCGCTGCCGCAGATGATGGAGAAGCTCGAGCGGCTCGGCT
GCTCCAAGCCGGTCGTCGGCCTGGTCGTACCGACCGGCTACTCGTTCAATCTCGACGGCACCAACATCTACATGACGCTG
GCGACCTTGTTCATCGCCCAGGCGATGAACGTGCACCTCTCCTTCGGCGAGCAGATCACGATCCTGCTCGTGGCCATGCT
GACCTCGAAGGGCGCCTCCGGCATCACCGGCGCGGGCTTCATTACCCTGGCCGGCACGCTCGCCGCTGTGCGGCCGGAGC
TCGTTCCGGGCATGGCCATCGTGCTCGGCATCGACAAGTTCATGAGCGAGTGCCGCGCGCTGACCAATCTCTGCGGCAAC
GGCGTCGCCTGCGTGATCGTCGCCTGGTGGGAGGGCGAGCTCGACCGCGACAAGCTGCGCGCGGCGCTCGACCGCGACAT
CGATCCGTCGGATGTCGAGGTCGCCGTCACGACCGGCTGA

Upstream 100 bases:

>100_bases
CGCGCTCGTGGACGTGTGACTTCGGCCCGGCTTGCAGGGAGAAGCGGACGTCACCGGCGAGACGAGATTGAGACGCTTGA
GACCAGGGAGATCGAATTCA

Downstream 100 bases:

>100_bases
AGCCGACGCCGCTCGCGCGCGGCGACGCCAGGCGGCGCGTCGCCAGCTCGCGGCGATCCCGAGCTGAACCGGCGGACGCG
CCGGCGAGGCCCAATCGTGA

Product: C4-dicarboxylate transport protein

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSATTPAAAAPKKPFYRVLYIQVLVAIVLGVIVGWLWPEIGKNDWIKALGDGFVKLIKMVIAPVIFCTVVSGIAHVSEVK
KVGRVAIKALIYFEIVSTFALALGLIVANVIQPGAGFQGQTNAAAVANYAKQASEMKSVDFVLHIIPDTVVGAFAQGEIL
QVLLFSILFGIALMSLGERGHTMRAFVDDAAHAIFGVIAIVVKAAPIGAFGAMAFTIGRYGPQALGNLAGLIATFYLTAL
AFVIIVLGIIARIAGFSIFKFLTYIKDELLIVLGTSSSESALPQMMEKLERLGCSKPVVGLVVPTGYSFNLDGTNIYMTL
ATLFIAQAMNVHLSFGEQITILLVAMLTSKGASGITGAGFITLAGTLAAVRPELVPGMAIVLGIDKFMSECRALTNLCGN
GVACVIVAWWEGELDRDKLRAALDRDIDPSDVEVAVTTG

Sequences:

>Translated_439_residues
MSATTPAAAAPKKPFYRVLYIQVLVAIVLGVIVGWLWPEIGKNDWIKALGDGFVKLIKMVIAPVIFCTVVSGIAHVSEVK
KVGRVAIKALIYFEIVSTFALALGLIVANVIQPGAGFQGQTNAAAVANYAKQASEMKSVDFVLHIIPDTVVGAFAQGEIL
QVLLFSILFGIALMSLGERGHTMRAFVDDAAHAIFGVIAIVVKAAPIGAFGAMAFTIGRYGPQALGNLAGLIATFYLTAL
AFVIIVLGIIARIAGFSIFKFLTYIKDELLIVLGTSSSESALPQMMEKLERLGCSKPVVGLVVPTGYSFNLDGTNIYMTL
ATLFIAQAMNVHLSFGEQITILLVAMLTSKGASGITGAGFITLAGTLAAVRPELVPGMAIVLGIDKFMSECRALTNLCGN
GVACVIVAWWEGELDRDKLRAALDRDIDPSDVEVAVTTG
>Mature_438_residues
SATTPAAAAPKKPFYRVLYIQVLVAIVLGVIVGWLWPEIGKNDWIKALGDGFVKLIKMVIAPVIFCTVVSGIAHVSEVKK
VGRVAIKALIYFEIVSTFALALGLIVANVIQPGAGFQGQTNAAAVANYAKQASEMKSVDFVLHIIPDTVVGAFAQGEILQ
VLLFSILFGIALMSLGERGHTMRAFVDDAAHAIFGVIAIVVKAAPIGAFGAMAFTIGRYGPQALGNLAGLIATFYLTALA
FVIIVLGIIARIAGFSIFKFLTYIKDELLIVLGTSSSESALPQMMEKLERLGCSKPVVGLVVPTGYSFNLDGTNIYMTLA
TLFIAQAMNVHLSFGEQITILLVAMLTSKGASGITGAGFITLAGTLAAVRPELVPGMAIVLGIDKFMSECRALTNLCGNG
VACVIVAWWEGELDRDKLRAALDRDIDPSDVEVAVTTG

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI169790839, Length=479, Percent_Identity=26.3048016701461, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4827012, Length=416, Percent_Identity=25.2403846153846, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI194239697, Length=476, Percent_Identity=23.9495798319328, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI262359914, Length=411, Percent_Identity=25.5474452554745, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI40254478, Length=449, Percent_Identity=24.2761692650334, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI5032093, Length=395, Percent_Identity=26.5822784810127, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI21314632, Length=400, Percent_Identity=25.75, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI66773030, Length=444, Percent_Identity=23.4234234234234, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI223468566, Length=278, Percent_Identity=29.136690647482, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI223468564, Length=270, Percent_Identity=28.8888888888889, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI301601644, Length=151, Percent_Identity=27.8145695364238, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789947, Length=417, Percent_Identity=58.0335731414868, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1790514, Length=419, Percent_Identity=36.9928400954654, Blast_Score=284, Evalue=9e-78,
Organism=Escherichia coli, GI1788024, Length=406, Percent_Identity=21.4285714285714, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789473, Length=315, Percent_Identity=22.8571428571429, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17537407, Length=441, Percent_Identity=24.9433106575964, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71996953, Length=441, Percent_Identity=26.3038548752834, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71983099, Length=468, Percent_Identity=25.6410256410256, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71983106, Length=468, Percent_Identity=25.6410256410256, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI193206654, Length=450, Percent_Identity=22.2222222222222, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI193206505, Length=451, Percent_Identity=23.2815964523282, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17541374, Length=447, Percent_Identity=24.3847874720358, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24583025, Length=463, Percent_Identity=22.4622030237581, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17137668, Length=463, Percent_Identity=22.4622030237581, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24583023, Length=463, Percent_Identity=22.4622030237581, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI281360481, Length=466, Percent_Identity=22.7467811158798, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281360483, Length=466, Percent_Identity=22.7467811158798, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17137666, Length=466, Percent_Identity=22.7467811158798, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA1_BRASO (A4YQF7)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001204370.1
- ProteinModelPortal:   A4YQF7
- SMR:   A4YQF7
- GeneID:   5119636
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO2301
- eggNOG:   COG1301
- HOGENOM:   HBG739804
- OMA:   EQITILL
- ProtClustDB:   CLSK818651
- BioCyc:   BSP376:BRADO2301-MONOMER
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 46398; Mature: 46266

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x18aa42d0)-; HASH(0x19d14d30)-; HASH(0x40a37acc)-; HASH(0x1a34da24)-; HASH(0x40a8ee38)-; HASH(0x40a755fc)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCCCEEEEECH
>Mature Secondary Structure 
SATTPAAAAPKKPFYRVLYIQVLVAIVLGVIVGWLWPEIGKNDWIKALGDGFVKLIKMV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IAPVIFCTVVSGIAHVSEVKKVGRVAIKALIYFEIVSTFALALGLIVANVIQPGAGFQGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TNAAAVANYAKQASEMKSVDFVLHIIPDTVVGAFAQGEILQVLLFSILFGIALMSLGERG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
HTMRAFVDDAAHAIFGVIAIVVKAAPIGAFGAMAFTIGRYGPQALGNLAGLIATFYLTAL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AFVIIVLGIIARIAGFSIFKFLTYIKDELLIVLGTSSSESALPQMMEKLERLGCSKPVVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEE
LVVPTGYSFNLDGTNIYMTLATLFIAQAMNVHLSFGEQITILLVAMLTSKGASGITGAGF
EEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHH
ITLAGTLAAVRPELVPGMAIVLGIDKFMSECRALTNLCGNGVACVIVAWWEGELDRDKLR
HHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHH
AALDRDIDPSDVEVAVTTG
HHHCCCCCCCCCEEEEECH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA