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The map label for this gene is wcaI [H]

Identifier: 146339235

GI number: 146339235

Start: 2333192

End: 2334478

Strand: Reverse

Name: wcaI [H]

Synonym: BRADO2201

Alternate gene names: 146339235

Gene position: 2334478-2333192 (Counterclockwise)

Preceding gene: 146339236

Following gene: 146339233

Centisome position: 31.31

GC content: 64.02

Gene sequence:

>1287_bases
ATGCGCCTCTTGGTGATCGGCATCAATTATGCCCCAGATCTGATCGGCGTCGCCAAATACAATTCAGAGCTGTGTGAGGG
GCTGGCCTCGCTGGGCCATGAGGTCCGCATCGTTACGGCTCCCCCCTATTATCCGGACTGGGTCATCCCGGCGGGCTATC
GTTCATTATGGTATGAAAGGGAAAGGTCGTACGGCGTCGATGTCATCCGGGCGCCGATCTATGTTCCCAAGTCGCCGTCC
GGCTTCAAACGTATCGTCCATCACGGTTCGTTCCTGCTTTCCGCGTCGGCTCCCGTCCTCAGCAGCGCGATGCGCTGGCG
GCCGGACTTGGTCCTGGCCGTTGCACCGTCGCTGCTCTCGGCCTCTCTCGCCGCGGCTGCCGCCCGTGTTGCCAATGCAT
CCTGCTGGCTCCACGTCCAGGACTTCGAGGTCGATGCAGCCTTCGAACTCGGACTACTCGGAGCGAACCCGAGAATGCGC
GCCGCAATGCTCGCACTCGAGCGACGCCTGATGGATGCGTTCGACCACGTCTCGACGATCTCGCCGCACATGTTGCGCTG
CCTGCGCAAGAAGGGGCTCGCCGCCGCGAAGCTGTCCGAGTTGCGCAACTGGATCGATACGGAAGCGATTGCTCCCGGCC
GCCACGACACCGCCTTCCGTGCGCAGCTCGGGCTTGATACGTCCGACGTGATCGCCCTTTATTCCGGCGCAATGTCCAAC
AAGCAAGGCCTCGACCTCGTGATCGACATGGCTGCCAGCCTGCGCGAGCGCTGCTCGCTGCATTTCGTGCTTTGCGGAAA
TGGGCCGATCAAGGCCGAACTGATGCGCATGGCCGGAGGGCTCGGCAACGTCCATTTCCTCGATCTGCAACCTTCCGAGC
GGCTGAGCGAATTGCTCAGTACCGCCGATTTTCATCTTCTGCCGCAGAAGGCGCAGATCTCGGACCTCGTGCTCCCGTCG
AAGCTCGCGGGAATGCTCGCGTCCGGCCGCCCAGTGGTCGCGATGACCACCCCCGGCTCCGGCATCGCGGAGGAGATCGA
AGGCGCGGGTCTGATCGTTCCGCCGGGCGATGTCGGCGCGCTGTCAGCCGCCGTCGTGCGCCTCTCGAAGGACCAGGACA
TGCGCGCCGCCCTCGGCCGCGTTGCACGGCTACGCGCCCGTCAAAAATGGGATCGGTCGGTCGTCATTCGCAATATCGAG
CGCGAATTCATGGCGGTCGCGGAGAGCAGGCCTCGGTCCCGATCGACACCGTCCGAGGCAGCGCGCCCGCTGCCCTCCGC
CGATTAA

Upstream 100 bases:

>100_bases
CATCGTCGACGCTCGACATCAGCGACCCGGCCGCCTTCGGATCTGCCGAGATACGTTCGATCGGGGCGAGCAAATTGCAT
TGGCAGATCCGAGGAACGTC

Downstream 100 bases:

>100_bases
GCGACAGCCGACGATGACGACGCACGTGAGTGCGCAGCGTCGGCGCAAGTCTGCGGCTGTGAGGGATCGTTCGACAGGCG
ACCGGGACATATTACCGGGG

Product: putative glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MRLLVIGINYAPDLIGVAKYNSELCEGLASLGHEVRIVTAPPYYPDWVIPAGYRSLWYERERSYGVDVIRAPIYVPKSPS
GFKRIVHHGSFLLSASAPVLSSAMRWRPDLVLAVAPSLLSASLAAAAARVANASCWLHVQDFEVDAAFELGLLGANPRMR
AAMLALERRLMDAFDHVSTISPHMLRCLRKKGLAAAKLSELRNWIDTEAIAPGRHDTAFRAQLGLDTSDVIALYSGAMSN
KQGLDLVIDMAASLRERCSLHFVLCGNGPIKAELMRMAGGLGNVHFLDLQPSERLSELLSTADFHLLPQKAQISDLVLPS
KLAGMLASGRPVVAMTTPGSGIAEEIEGAGLIVPPGDVGALSAAVVRLSKDQDMRAALGRVARLRARQKWDRSVVIRNIE
REFMAVAESRPRSRSTPSEAARPLPSAD

Sequences:

>Translated_428_residues
MRLLVIGINYAPDLIGVAKYNSELCEGLASLGHEVRIVTAPPYYPDWVIPAGYRSLWYERERSYGVDVIRAPIYVPKSPS
GFKRIVHHGSFLLSASAPVLSSAMRWRPDLVLAVAPSLLSASLAAAAARVANASCWLHVQDFEVDAAFELGLLGANPRMR
AAMLALERRLMDAFDHVSTISPHMLRCLRKKGLAAAKLSELRNWIDTEAIAPGRHDTAFRAQLGLDTSDVIALYSGAMSN
KQGLDLVIDMAASLRERCSLHFVLCGNGPIKAELMRMAGGLGNVHFLDLQPSERLSELLSTADFHLLPQKAQISDLVLPS
KLAGMLASGRPVVAMTTPGSGIAEEIEGAGLIVPPGDVGALSAAVVRLSKDQDMRAALGRVARLRARQKWDRSVVIRNIE
REFMAVAESRPRSRSTPSEAARPLPSAD
>Mature_428_residues
MRLLVIGINYAPDLIGVAKYNSELCEGLASLGHEVRIVTAPPYYPDWVIPAGYRSLWYERERSYGVDVIRAPIYVPKSPS
GFKRIVHHGSFLLSASAPVLSSAMRWRPDLVLAVAPSLLSASLAAAAARVANASCWLHVQDFEVDAAFELGLLGANPRMR
AAMLALERRLMDAFDHVSTISPHMLRCLRKKGLAAAKLSELRNWIDTEAIAPGRHDTAFRAQLGLDTSDVIALYSGAMSN
KQGLDLVIDMAASLRERCSLHFVLCGNGPIKAELMRMAGGLGNVHFLDLQPSERLSELLSTADFHLLPQKAQISDLVLPS
KLAGMLASGRPVVAMTTPGSGIAEEIEGAGLIVPPGDVGALSAAVVRLSKDQDMRAALGRVARLRARQKWDRSVVIRNIE
REFMAVAESRPRSRSTPSEAARPLPSAD

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788363, Length=415, Percent_Identity=40, Blast_Score=239, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 46380; Mature: 46380

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLVIGINYAPDLIGVAKYNSELCEGLASLGHEVRIVTAPPYYPDWVIPAGYRSLWYER
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
ERSYGVDVIRAPIYVPKSPSGFKRIVHHGSFLLSASAPVLSSAMRWRPDLVLAVAPSLLS
HHCCCCEEEECCEECCCCCHHHHHHHHCCCEEEECCCHHHHHHHHCCCCEEEEHHHHHHH
ASLAAAAARVANASCWLHVQDFEVDAAFELGLLGANPRMRAAMLALERRLMDAFDHVSTI
HHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
SPHMLRCLRKKGLAAAKLSELRNWIDTEAIAPGRHDTAFRAQLGLDTSDVIALYSGAMSN
CHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHCCCCC
KQGLDLVIDMAASLRERCSLHFVLCGNGPIKAELMRMAGGLGNVHFLDLQPSERLSELLS
CCCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
TADFHLLPQKAQISDLVLPSKLAGMLASGRPVVAMTTPGSGIAEEIEGAGLIVPPGDVGA
HCCCEECCCCCCHHHHCCHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCEEEECCCCHHH
LSAAVVRLSKDQDMRAALGRVARLRARQKWDRSVVIRNIEREFMAVAESRPRSRSTPSEA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ARPLPSAD
HCCCCCCC
>Mature Secondary Structure
MRLLVIGINYAPDLIGVAKYNSELCEGLASLGHEVRIVTAPPYYPDWVIPAGYRSLWYER
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
ERSYGVDVIRAPIYVPKSPSGFKRIVHHGSFLLSASAPVLSSAMRWRPDLVLAVAPSLLS
HHCCCCEEEECCEECCCCCHHHHHHHHCCCEEEECCCHHHHHHHHCCCCEEEEHHHHHHH
ASLAAAAARVANASCWLHVQDFEVDAAFELGLLGANPRMRAAMLALERRLMDAFDHVSTI
HHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
SPHMLRCLRKKGLAAAKLSELRNWIDTEAIAPGRHDTAFRAQLGLDTSDVIALYSGAMSN
CHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHCCCCC
KQGLDLVIDMAASLRERCSLHFVLCGNGPIKAELMRMAGGLGNVHFLDLQPSERLSELLS
CCCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
TADFHLLPQKAQISDLVLPSKLAGMLASGRPVVAMTTPGSGIAEEIEGAGLIVPPGDVGA
HCCCEECCCCCCHHHHCCHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCEEEECCCCHHH
LSAAVVRLSKDQDMRAALGRVARLRARQKWDRSVVIRNIEREFMAVAESRPRSRSTPSEA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ARPLPSAD
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503 [H]