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The map label for this gene is rpoE [H]

Identifier: 146339190

GI number: 146339190

Start: 2286182

End: 2286670

Strand: Reverse

Name: rpoE [H]

Synonym: BRADO2150

Alternate gene names: 146339190

Gene position: 2286670-2286182 (Counterclockwise)

Preceding gene: 146339195

Following gene: 146339189

Centisome position: 30.67

GC content: 70.35

Gene sequence:

>489_bases
ATGAGCGCGTTTCGCCAGAGCGTCGAGGCCATGATTCCGGCACTGCGCCGCTATGCTCGCGCCTTGGCGCGCGACATGGA
CGCCGCTGACGACCTGGTCCAGGACACCCTGGTGCGGGCGCTGCGGTCCGAGCGGCTCTTCCTCGGCGGCGACCTGCGCA
GCTGGCTCTATACGATCCTCACCAACCTCAACAAGAACCGGCGCCGCTCGCTGGCGCGGCGGCCACACTTCATGCCGCTG
CAGGACGACAATCCCGATGCCAGCGGCACCGAGGCCGAGGGCCGCGACATCGAGCGCGCGCTGGCGACCCTGGTAGAGGA
GCAACGCGCCGTGCTGCTGCTCGTCGTGCTCGAGGGGCTGAGCTACCGCGAGGTGGCCGACATCCAGGGTGTCCCGATCG
GCACCGTGATGTCGCGCCTGGCCCGTGCCCGCGCTCATGTCCGCGCCGTCCTCGAGGGCGAGCGCCCGGCGCTGCGGAGG
GTGAAATGA

Upstream 100 bases:

>100_bases
CCGGGGCGGAATAGGTCTGCCATCGCGGGGTTATGACCATTGCAAGGCGCGGTTGCGGCCCGCGCCCTGGGCACAGAAAG
GCACGGCCGCAGGACAGTCC

Downstream 100 bases:

>100_bases
GGCCGCCGTTCACCGTACAAAAGCCGCTCTGCCGCGTTCGCCTGCCCAAGGCGCCTGTGGCCGCCGATCGTCGCGGCCGC
CAGACTGCAACGAGATCCAG

Product: ECF family RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MSAFRQSVEAMIPALRRYARALARDMDAADDLVQDTLVRALRSERLFLGGDLRSWLYTILTNLNKNRRRSLARRPHFMPL
QDDNPDASGTEAEGRDIERALATLVEEQRAVLLLVVLEGLSYREVADIQGVPIGTVMSRLARARAHVRAVLEGERPALRR
VK

Sequences:

>Translated_162_residues
MSAFRQSVEAMIPALRRYARALARDMDAADDLVQDTLVRALRSERLFLGGDLRSWLYTILTNLNKNRRRSLARRPHFMPL
QDDNPDASGTEAEGRDIERALATLVEEQRAVLLLVVLEGLSYREVADIQGVPIGTVMSRLARARAHVRAVLEGERPALRR
VK
>Mature_161_residues
SAFRQSVEAMIPALRRYARALARDMDAADDLVQDTLVRALRSERLFLGGDLRSWLYTILTNLNKNRRRSLARRPHFMPLQ
DDNPDASGTEAEGRDIERALATLVEEQRAVLLLVVLEGLSYREVADIQGVPIGTVMSRLARARAHVRAVLEGERPALRRV
K

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=147, Percent_Identity=31.9727891156463, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 18302; Mature: 18171

Theoretical pI: Translated: 10.81; Mature: 10.81

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFRQSVEAMIPALRRYARALARDMDAADDLVQDTLVRALRSERLFLGGDLRSWLYTIL
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TNLNKNRRRSLARRPHFMPLQDDNPDASGTEAEGRDIERALATLVEEQRAVLLLVVLEGL
HHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
SYREVADIQGVPIGTVMSRLARARAHVRAVLEGERPALRRVK
CHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure 
SAFRQSVEAMIPALRRYARALARDMDAADDLVQDTLVRALRSERLFLGGDLRSWLYTIL
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TNLNKNRRRSLARRPHFMPLQDDNPDASGTEAEGRDIERALATLVEEQRAVLLLVVLEGL
HHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
SYREVADIQGVPIGTVMSRLARARAHVRAVLEGERPALRRVK
CHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]