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The map label for this gene is potD [H]

Identifier: 146339152

GI number: 146339152

Start: 2243309

End: 2244373

Strand: Reverse

Name: potD [H]

Synonym: BRADO2110

Alternate gene names: 146339152

Gene position: 2244373-2243309 (Counterclockwise)

Preceding gene: 146339153

Following gene: 146339151

Centisome position: 30.1

GC content: 64.23

Gene sequence:

>1065_bases
ATGACCGCCTCATCGCGTCGCTTCCCCCTCAGCCGCCGCCGCCTGCTGACCGCGGCAGCTTCGCTCGGCGCCGGCTTCGT
CGCGGCGCCTTATGTCGCGCGCGCCGAGGAGCCGGTGCTGAACGTCACCGGCTGGGGCGGCAAATGGGGCGAGGTGATGA
AGGCCGAGATCGGCCTGGCCTTCGAGAGCGAGTTCAAGTGCAAGCTCAAGACCGACACCGCGCTGCCCTTCCTGCCGAAG
CTGCAGGCGAGCTCGCGCTCCGCGCCGGTCTACGACGTGCTGCACACCAACTCCAACGAGCAATGGGCCGCCGTCGAGAT
GGGGCTCGTCGAGCCGAAGGTCGACCCCAAGCTGGTGCCGAACATCGCCGACGTCTATCCCTACGCGGTCAGCGACAAGA
TCGTCGGCGTCTGCATCTTCACCAGCGCCATCGGCCTGGGCATGCGCACCGACAAGGGCTATGGCAGCGTCACGTCGTGG
AAGGACTATTGGGACGCCAAGTTCAACGGCGCCCGCGGCGGATACGTCATCCCCGCGAACAGCCTCGGACAGGCGTTCGT
GATGATGTGCGGCACGCTCTACGGCAAGGGCCAGACCGATCTCGATGCCGCCTATGCGGCGTTGGAGAAGCTGAAGCCGA
TCAAGCTGGTCGATTTCACCGGCGCGATGGAGAAGCTGATCCTGTCGGGCGAGGCCGGCATCGGCATCCTCCACGATTCC
GGCATCTATCGGTATGACGGGCAGAACCAGCCGATCGACTTCGTCACGCCGAGCGAGGGCGTGCTGTCGCTCGAGCAGGT
GCTGAGCATCACGCCGGGCAGCAAGGTGAAGGAACTCGGCAACGCCTACATCAACTACATGCTGCGCCCGGATGTTCAAA
AGCGCCTCGCCGAAACCGTGTGGTACTCGCCGGCCAACAAGAAGGTGAAGCTCGACGCCAAGTATGACGCGAAGCTTCTG
ACCACGCCGGAGAAGGTCGGCAAGCTGATCCAGGTCGACTGGAAGTGGTACAACGCGCAGAAGGACGAGATCGACAGCAG
GGTCAACCGGATCTTCCGTGCATGA

Upstream 100 bases:

>100_bases
CCTCCGTCATTTCGATCCTCCTCGCCTTCATCGTCGTCATCGCGCTCGACCGCACCGTTGGCCTGCGGTCCACCGTCAAA
CTGTAAGGGAGCTCCCGGAT

Downstream 100 bases:

>100_bases
GCGCTGCGATCGAGATTGCCGGGGTCAGCAAGATCTATGACGGCGGCGTGCGCGCCGTCGATGCGGTGGCGATGGACATC
AGGCCCGGCGAATTCTTTTC

Product: putative spermidine/putrescine-binding periplasmic protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MTASSRRFPLSRRRLLTAAASLGAGFVAAPYVARAEEPVLNVTGWGGKWGEVMKAEIGLAFESEFKCKLKTDTALPFLPK
LQASSRSAPVYDVLHTNSNEQWAAVEMGLVEPKVDPKLVPNIADVYPYAVSDKIVGVCIFTSAIGLGMRTDKGYGSVTSW
KDYWDAKFNGARGGYVIPANSLGQAFVMMCGTLYGKGQTDLDAAYAALEKLKPIKLVDFTGAMEKLILSGEAGIGILHDS
GIYRYDGQNQPIDFVTPSEGVLSLEQVLSITPGSKVKELGNAYINYMLRPDVQKRLAETVWYSPANKKVKLDAKYDAKLL
TTPEKVGKLIQVDWKWYNAQKDEIDSRVNRIFRA

Sequences:

>Translated_354_residues
MTASSRRFPLSRRRLLTAAASLGAGFVAAPYVARAEEPVLNVTGWGGKWGEVMKAEIGLAFESEFKCKLKTDTALPFLPK
LQASSRSAPVYDVLHTNSNEQWAAVEMGLVEPKVDPKLVPNIADVYPYAVSDKIVGVCIFTSAIGLGMRTDKGYGSVTSW
KDYWDAKFNGARGGYVIPANSLGQAFVMMCGTLYGKGQTDLDAAYAALEKLKPIKLVDFTGAMEKLILSGEAGIGILHDS
GIYRYDGQNQPIDFVTPSEGVLSLEQVLSITPGSKVKELGNAYINYMLRPDVQKRLAETVWYSPANKKVKLDAKYDAKLL
TTPEKVGKLIQVDWKWYNAQKDEIDSRVNRIFRA
>Mature_353_residues
TASSRRFPLSRRRLLTAAASLGAGFVAAPYVARAEEPVLNVTGWGGKWGEVMKAEIGLAFESEFKCKLKTDTALPFLPKL
QASSRSAPVYDVLHTNSNEQWAAVEMGLVEPKVDPKLVPNIADVYPYAVSDKIVGVCIFTSAIGLGMRTDKGYGSVTSWK
DYWDAKFNGARGGYVIPANSLGQAFVMMCGTLYGKGQTDLDAAYAALEKLKPIKLVDFTGAMEKLILSGEAGIGILHDSG
IYRYDGQNQPIDFVTPSEGVLSLEQVLSITPGSKVKELGNAYINYMLRPDVQKRLAETVWYSPANKKVKLDAKYDAKLLT
TPEKVGKLIQVDWKWYNAQKDEIDSRVNRIFRA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=155, Percent_Identity=30.9677419354839, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38893; Mature: 38762

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS00697 DNA_LIGASE_A1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASSRRFPLSRRRLLTAAASLGAGFVAAPYVARAEEPVLNVTGWGGKWGEVMKAEIGLA
CCCCCCCCCHHHHHHHHHHHHHCCCHHHCCHHHCCCCCEEEEECCCCCHHHHHHHHHCEE
FESEFKCKLKTDTALPFLPKLQASSRSAPVYDVLHTNSNEQWAAVEMGLVEPKVDPKLVP
ECCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCC
NIADVYPYAVSDKIVGVCIFTSAIGLGMRTDKGYGSVTSWKDYWDAKFNGARGGYVIPAN
CHHHHCCCHHCCCCEEHHEEHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEECC
SLGQAFVMMCGTLYGKGQTDLDAAYAALEKLKPIKLVDFTGAMEKLILSGEAGIGILHDS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCEEEEECC
GIYRYDGQNQPIDFVTPSEGVLSLEQVLSITPGSKVKELGNAYINYMLRPDVQKRLAETV
CEEEECCCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCHHHHHHHHH
WYSPANKKVKLDAKYDAKLLTTPEKVGKLIQVDWKWYNAQKDEIDSRVNRIFRA
EECCCCCEEEEECCCCCEEECCHHHHCCEEEEEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TASSRRFPLSRRRLLTAAASLGAGFVAAPYVARAEEPVLNVTGWGGKWGEVMKAEIGLA
CCCCCCCCHHHHHHHHHHHHHCCCHHHCCHHHCCCCCEEEEECCCCCHHHHHHHHHCEE
FESEFKCKLKTDTALPFLPKLQASSRSAPVYDVLHTNSNEQWAAVEMGLVEPKVDPKLVP
ECCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCC
NIADVYPYAVSDKIVGVCIFTSAIGLGMRTDKGYGSVTSWKDYWDAKFNGARGGYVIPAN
CHHHHCCCHHCCCCEEHHEEHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEECC
SLGQAFVMMCGTLYGKGQTDLDAAYAALEKLKPIKLVDFTGAMEKLILSGEAGIGILHDS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCEEEEECC
GIYRYDGQNQPIDFVTPSEGVLSLEQVLSITPGSKVKELGNAYINYMLRPDVQKRLAETV
CEEEECCCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCHHHHHHHHH
WYSPANKKVKLDAKYDAKLLTTPEKVGKLIQVDWKWYNAQKDEIDSRVNRIFRA
EECCCCCEEEEECCCCCEEECCHHHHCCEEEEEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]