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The map label for this gene is pncA [H]

Identifier: 146339148

GI number: 146339148

Start: 2238829

End: 2239464

Strand: Direct

Name: pncA [H]

Synonym: BRADO2106

Alternate gene names: 146339148

Gene position: 2238829-2239464 (Clockwise)

Preceding gene: 146339146

Following gene: 146339158

Centisome position: 30.02

GC content: 65.25

Gene sequence:

>636_bases
GTGTGGATAGGAATGCAGATCAAGACCCATGAGGATGTGCTGCTCGTCATCGACGTGCAGAACGATTTTTGTCCGGGCGG
GCAGCTTGCCGTCGCCGAGGGCGACGCCGTCGTGCCCGTCATCAACCGGCTGAGCGGGCTGTTCGATCACGTCGTGCTGA
CCCAGGACTGGCATCCGGCAGGCCACAGCTCGTTCGCCTCCAGCCACCCCGGTAAGGCGCCGTTCGAGAGCGTGACCATG
CCCTATGGGCCGCAGACCCTGTGGCCGGATCATTGCATCCAGGGCACAAAGGGGGCGGCGTTTCATGACGATCTCGCGAC
AGACAAGGCGCAGCTGATCATCCGCAAGGGGTTTCGCGCGGCGATCGATTCCTATTCCGCGTTCTTCGAGAACGACAAGA
CCACGCCCACAGGCCTCGCCGGCTATTTGCGCGAGCGCGGCCTGAAGCGGGTGTTCCTGGTCGGGCTCGCGACCGATTTC
TGCGTGCACTACTCGGCGGTCGACGCGCGGCGGCTCGGCTTTTCGGCTGTTGTCATCGACAACGCGTGCCGCGGCATCGA
TCTCGGCGGCTCGATGGCAGCGGCCAAGGCGCAAGGCGCGGAGGCCGGCGTGGAGCGGATCGCGGAGCTCGTGTGA

Upstream 100 bases:

>100_bases
TTCCGAATTGGAGGGGCTCCTCCCCCAGGCAGAATCTTCGCTGTCCCGGCTTGAGATCGGCCCCGGCTGCGGTCATCCTC
GGGTCAATTCAGGCAAGCGG

Downstream 100 bases:

>100_bases
GATGAGATGGTGAGGCTGGCGATGCGGCTGAGCTACGCCGCCGCATCGATGTCGGGCTCGGCGATGGCGCCGGCGACGCG
GACGCGGACACGGAGTGCAT

Product: nicotinamidase/pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MWIGMQIKTHEDVLLVIDVQNDFCPGGQLAVAEGDAVVPVINRLSGLFDHVVLTQDWHPAGHSSFASSHPGKAPFESVTM
PYGPQTLWPDHCIQGTKGAAFHDDLATDKAQLIIRKGFRAAIDSYSAFFENDKTTPTGLAGYLRERGLKRVFLVGLATDF
CVHYSAVDARRLGFSAVVIDNACRGIDLGGSMAAAKAQGAEAGVERIAELV

Sequences:

>Translated_211_residues
MWIGMQIKTHEDVLLVIDVQNDFCPGGQLAVAEGDAVVPVINRLSGLFDHVVLTQDWHPAGHSSFASSHPGKAPFESVTM
PYGPQTLWPDHCIQGTKGAAFHDDLATDKAQLIIRKGFRAAIDSYSAFFENDKTTPTGLAGYLRERGLKRVFLVGLATDF
CVHYSAVDARRLGFSAVVIDNACRGIDLGGSMAAAKAQGAEAGVERIAELV
>Mature_211_residues
MWIGMQIKTHEDVLLVIDVQNDFCPGGQLAVAEGDAVVPVINRLSGLFDHVVLTQDWHPAGHSSFASSHPGKAPFESVTM
PYGPQTLWPDHCIQGTKGAAFHDDLATDKAQLIIRKGFRAAIDSYSAFFENDKTTPTGLAGYLRERGLKRVFLVGLATDF
CVHYSAVDARRLGFSAVVIDNACRGIDLGGSMAAAKAQGAEAGVERIAELV

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=179, Percent_Identity=44.1340782122905, Blast_Score=163, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI193206735, Length=214, Percent_Identity=36.4485981308411, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17543130, Length=214, Percent_Identity=36.4485981308411, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71999339, Length=197, Percent_Identity=32.994923857868, Blast_Score=93, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6321401, Length=190, Percent_Identity=33.6842105263158, Blast_Score=107, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648247, Length=210, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22668; Mature: 22668

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWIGMQIKTHEDVLLVIDVQNDFCPGGQLAVAEGDAVVPVINRLSGLFDHVVLTQDWHPA
CEECCEEECCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHEEECCCCCC
GHSSFASSHPGKAPFESVTMPYGPQTLWPDHCIQGTKGAAFHDDLATDKAQLIIRKGFRA
CCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AIDSYSAFFENDKTTPTGLAGYLRERGLKRVFLVGLATDFCVHYSAVDARRLGFSAVVID
HHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCEEEEEEC
NACRGIDLGGSMAAAKAQGAEAGVERIAELV
CCCCCCCCCCCHHHHHHCCHHHHHHHHHHHC
>Mature Secondary Structure
MWIGMQIKTHEDVLLVIDVQNDFCPGGQLAVAEGDAVVPVINRLSGLFDHVVLTQDWHPA
CEECCEEECCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHEEECCCCCC
GHSSFASSHPGKAPFESVTMPYGPQTLWPDHCIQGTKGAAFHDDLATDKAQLIIRKGFRA
CCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AIDSYSAFFENDKTTPTGLAGYLRERGLKRVFLVGLATDFCVHYSAVDARRLGFSAVVID
HHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCEEEEEEC
NACRGIDLGGSMAAAKAQGAEAGVERIAELV
CCCCCCCCCCCHHHHHHCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]