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The map label for this gene is bam [H]

Identifier: 146339134

GI number: 146339134

Start: 2223962

End: 2225386

Strand: Reverse

Name: bam [H]

Synonym: BRADO2088

Alternate gene names: 146339134

Gene position: 2225386-2223962 (Counterclockwise)

Preceding gene: 146339138

Following gene: 146339130

Centisome position: 29.84

GC content: 70.18

Gene sequence:

>1425_bases
TTGGATCACAAAGGTTCGCTTCGCATGGATACCCCGCTGTGGCGCTGGTCGGCCACGCAACTCGCATCCGCCATCCGCAA
CAAAGAGGTCACGAGTCGCGAGGCCGTGACGGCCTGTCTCGACCGCATCGGCGCGGTCAATCCCGAGTTCAACGCCGTGG
TCTCGGTGCAGGCGGAGGAGGCGCTCGCGGCGGCCGACCTCGCTGACCGCATGGTGGCCGCGGGGACCACGCTGGGGCCG
CTGCACGGCGTGCCGGTGACGACCAAGATCAATGTCGACCAGAAGGGCCTGCCGACCACCAACGGCGTCGTCGCCTTCAA
GGACAACATCGCCGCGGACGACGCGCCGGTCGTCGCCAATCTGCGCAAGGCGGGCGCCGTGATCATCGGCCGCACCAACA
CGCCGGCATTCTCGATGCGCTGGTTCACCGACAACGAGCTGCACGGCCGCACGCTCAACCCGTGGCGGCCCGGCCGCACG
CCGGGCGGCTCCAGCGGCGGCGCGGCGGTCGCAGCGGCGACCGGCATGTGCGCGATCGCACATGGCAATGACGGCGGCGG
CTCGATCCGCTATCCGGCCTATTGCACCGGCACCGTGGGCCTGCGCCCATCCTTCGGCCGCGTGCCGGCATTCAACGGCA
CCGCGCCGTCCGAGCGGCAATTGTCGCTGCAGCTGATCTCGGTGCAGGGCGCGATCACGCGCAACGTCGAAGACGCAAGG
CTGGCGCTGGCCGCGATGGCCGCGCCCGATCCGCGCGATCCCTGGCACGTCGCGATGCCGCTCGAGGGCCCGCGCATCGA
TCATCCCGGCGTCGCCGCCTGCCTCGATCCCGCGGGCGTCGGCATGGCGCCGGAGGTCGAGGCGGCCGTGCGCAAGGCCG
CGGCGATCCTGCACCGCGCAGGCTATGAGGTGACGTGGCGCGATCCGCCGGATGTCGCCGCCGCTGCGCAGGCCTGGAAT
GATTTCGCACAAGGCGAGGCGCGCGTGACGCTGGCTTCGCAAGTGGAGCAGCATGGCGACGCGCTGGCGCGGCGCGCCTT
CGCGCTGATGATGAACCGCACGCCTGAGCTCGACGTCACCGGACTGATCAAGGCGTCTTCGGCGCGCGCGACCTATCTGC
GGCGCTGGCAGCTGTTCATGCAGGAGCACCCGCTGGTACTCTGCCCCGTGGCGATGGAGACGGCGCTGCCCTATGGCGTC
GATCTCGACAGCGACGCCAGCGTCGACCGGCTGTATCGCTCGCACGTCTTCCTGTTCGCGACCGCGTATCTCGGGCTGCC
GTCGATCAGCGTGCCGACGGGCGTGCACGAGGGCATTCCGATCGGCGTTCAAATCGTGGGACCGCGCTTCCGCGAGGATC
TCGTGCTGGATGCGGCCGCTGCGGTCGAGCATGCCGCCGCCGAGGCGGCGCCGACGCTATCGTAA

Upstream 100 bases:

>100_bases
GAACACAATTTTTCGCCCATGGCCGCCGAGTCACCTATGCTCGCGCGGTTAGGACGTGACGCTGGGGCTGCGCGCCCTGC
TTCGCGGCTGAATCATTGGT

Downstream 100 bases:

>100_bases
GCGTCCGGCATCACGTCAGCACATGAGAGGGCCGCGCGGGTTCAGGAGCTGCGCGCGCGGCTCCATTCGCTCTCCATTTT
TGCCGCATCGATCGCCGCCG

Product: indoleacetamide hydrolase

Products: NA

Alternate protein names: IAH; Indole-3-acetamide hydrolase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MDHKGSLRMDTPLWRWSATQLASAIRNKEVTSREAVTACLDRIGAVNPEFNAVVSVQAEEALAAADLADRMVAAGTTLGP
LHGVPVTTKINVDQKGLPTTNGVVAFKDNIAADDAPVVANLRKAGAVIIGRTNTPAFSMRWFTDNELHGRTLNPWRPGRT
PGGSSGGAAVAAATGMCAIAHGNDGGGSIRYPAYCTGTVGLRPSFGRVPAFNGTAPSERQLSLQLISVQGAITRNVEDAR
LALAAMAAPDPRDPWHVAMPLEGPRIDHPGVAACLDPAGVGMAPEVEAAVRKAAAILHRAGYEVTWRDPPDVAAAAQAWN
DFAQGEARVTLASQVEQHGDALARRAFALMMNRTPELDVTGLIKASSARATYLRRWQLFMQEHPLVLCPVAMETALPYGV
DLDSDASVDRLYRSHVFLFATAYLGLPSISVPTGVHEGIPIGVQIVGPRFREDLVLDAAAAVEHAAAEAAPTLS

Sequences:

>Translated_474_residues
MDHKGSLRMDTPLWRWSATQLASAIRNKEVTSREAVTACLDRIGAVNPEFNAVVSVQAEEALAAADLADRMVAAGTTLGP
LHGVPVTTKINVDQKGLPTTNGVVAFKDNIAADDAPVVANLRKAGAVIIGRTNTPAFSMRWFTDNELHGRTLNPWRPGRT
PGGSSGGAAVAAATGMCAIAHGNDGGGSIRYPAYCTGTVGLRPSFGRVPAFNGTAPSERQLSLQLISVQGAITRNVEDAR
LALAAMAAPDPRDPWHVAMPLEGPRIDHPGVAACLDPAGVGMAPEVEAAVRKAAAILHRAGYEVTWRDPPDVAAAAQAWN
DFAQGEARVTLASQVEQHGDALARRAFALMMNRTPELDVTGLIKASSARATYLRRWQLFMQEHPLVLCPVAMETALPYGV
DLDSDASVDRLYRSHVFLFATAYLGLPSISVPTGVHEGIPIGVQIVGPRFREDLVLDAAAAVEHAAAEAAPTLS
>Mature_474_residues
MDHKGSLRMDTPLWRWSATQLASAIRNKEVTSREAVTACLDRIGAVNPEFNAVVSVQAEEALAAADLADRMVAAGTTLGP
LHGVPVTTKINVDQKGLPTTNGVVAFKDNIAADDAPVVANLRKAGAVIIGRTNTPAFSMRWFTDNELHGRTLNPWRPGRT
PGGSSGGAAVAAATGMCAIAHGNDGGGSIRYPAYCTGTVGLRPSFGRVPAFNGTAPSERQLSLQLISVQGAITRNVEDAR
LALAAMAAPDPRDPWHVAMPLEGPRIDHPGVAACLDPAGVGMAPEVEAAVRKAAAILHRAGYEVTWRDPPDVAAAAQAWN
DFAQGEARVTLASQVEQHGDALARRAFALMMNRTPELDVTGLIKASSARATYLRRWQLFMQEHPLVLCPVAMETALPYGV
DLDSDASVDRLYRSHVFLFATAYLGLPSISVPTGVHEGIPIGVQIVGPRFREDLVLDAAAAVEHAAAEAAPTLS

Specific function: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid (IAA) [H]

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=498, Percent_Identity=28.5140562248996, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=25.748502994012, Blast_Score=135, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17537465, Length=294, Percent_Identity=30.6122448979592, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=306, Percent_Identity=29.0849673202614, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=321, Percent_Identity=29.595015576324, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=328, Percent_Identity=27.1341463414634, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17556276, Length=188, Percent_Identity=33.5106382978723, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=188, Percent_Identity=33.5106382978723, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=299, Percent_Identity=26.4214046822742, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320448, Length=228, Percent_Identity=30.2631578947368, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319685, Length=206, Percent_Identity=34.9514563106796, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=480, Percent_Identity=23.75, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24644968, Length=493, Percent_Identity=29.2089249492901, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI45550774, Length=289, Percent_Identity=33.5640138408304, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24648435, Length=289, Percent_Identity=33.5640138408304, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24648437, Length=289, Percent_Identity=33.5640138408304, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24652985, Length=241, Percent_Identity=37.344398340249, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922090, Length=241, Percent_Identity=37.344398340249, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24652981, Length=241, Percent_Identity=37.344398340249, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24652983, Length=241, Percent_Identity=37.344398340249, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078093, Length=496, Percent_Identity=26.6129032258064, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648441, Length=228, Percent_Identity=32.4561403508772, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648439, Length=228, Percent_Identity=32.4561403508772, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356731, Length=490, Percent_Identity=24.4897959183673, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24648113, Length=502, Percent_Identity=26.6932270916335, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 50049; Mature: 50049

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHKGSLRMDTPLWRWSATQLASAIRNKEVTSREAVTACLDRIGAVNPEFNAVVSVQAEE
CCCCCCEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHH
ALAAADLADRMVAAGTTLGPLHGVPVTTKINVDQKGLPTTNGVVAFKDNIAADDAPVVAN
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHH
LRKAGAVIIGRTNTPAFSMRWFTDNELHGRTLNPWRPGRTPGGSSGGAAVAAATGMCAIA
HHHCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEE
HGNDGGGSIRYPAYCTGTVGLRPSFGRVPAFNGTAPSERQLSLQLISVQGAITRNVEDAR
ECCCCCCEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCHHHCCCHHHH
LALAAMAAPDPRDPWHVAMPLEGPRIDHPGVAACLDPAGVGMAPEVEAAVRKAAAILHRA
HHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHC
GYEVTWRDPPDVAAAAQAWNDFAQGEARVTLASQVEQHGDALARRAFALMMNRTPELDVT
CCEEEECCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
GLIKASSARATYLRRWQLFMQEHPLVLCPVAMETALPYGVDLDSDASVDRLYRSHVFLFA
EEEECCCHHHHHHHHHHHHHHCCCEEEECHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
TAYLGLPSISVPTGVHEGIPIGVQIVGPRFREDLVLDAAAAVEHAAAEAAPTLS
HHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDHKGSLRMDTPLWRWSATQLASAIRNKEVTSREAVTACLDRIGAVNPEFNAVVSVQAEE
CCCCCCEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHH
ALAAADLADRMVAAGTTLGPLHGVPVTTKINVDQKGLPTTNGVVAFKDNIAADDAPVVAN
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHH
LRKAGAVIIGRTNTPAFSMRWFTDNELHGRTLNPWRPGRTPGGSSGGAAVAAATGMCAIA
HHHCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEE
HGNDGGGSIRYPAYCTGTVGLRPSFGRVPAFNGTAPSERQLSLQLISVQGAITRNVEDAR
ECCCCCCEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCHHHCCCHHHH
LALAAMAAPDPRDPWHVAMPLEGPRIDHPGVAACLDPAGVGMAPEVEAAVRKAAAILHRA
HHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHC
GYEVTWRDPPDVAAAAQAWNDFAQGEARVTLASQVEQHGDALARRAFALMMNRTPELDVT
CCEEEECCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
GLIKASSARATYLRRWQLFMQEHPLVLCPVAMETALPYGVDLDSDASVDRLYRSHVFLFA
EEEECCCHHHHHHHHHHHHHHCCCEEEECHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
TAYLGLPSISVPTGVHEGIPIGVQIVGPRFREDLVLDAAAAVEHAAAEAAPTLS
HHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]