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The map label for this gene is norB [H]

Identifier: 146338840

GI number: 146338840

Start: 1897431

End: 1898777

Strand: Direct

Name: norB [H]

Synonym: BRADO1777

Alternate gene names: 146338840

Gene position: 1897431-1898777 (Clockwise)

Preceding gene: 146338839

Following gene: 146338841

Centisome position: 25.45

GC content: 63.1

Gene sequence:

>1347_bases
ATGAGATATGAGACCCAGAAGGTCGCGATGCTGTACTTCTACGGCGCGCTGACGCTCTTCCTCGCCCAGGTCCTGTTCGG
CCTGCTCGCCGGCACCATCTACGTGCTGCCGAACACGCTGTCGGTCCTGCTGCCCTTCAACATCGTCCGCATGATCCACA
CCAACGCGCTGATCGTGTGGTCGCTGATCGGCTTCATGGGCGCGACCTATTTCCTACTGCCCGAGGAGACCGAGACCGAG
CTCTACAGCCCGCTGCTCGCCAAGATCCAGTTCTGGATGTTCTTCGGCGCCGCTGCTGCAGCCGTGGTCGGTTATCTCTT
CCACTACCACGAGGGCCGCGAGTTCCTCGAGCAGCCCTTCATCATCAAGGTCGGCATCGTCGTCGTCTGCCTGATGTTCC
TGTTCAACGTCACCATGACGGCGCTGAAGGGCCGCAAGACCACGGTGACCAACATCCTGCTGTTCGGGATGTGGGGGGTG
GCGATCTTCTTCCTGTTCGCGTTCTACAACCCGGCCAATCTCGCCGTCGACAAGATGTACTGGTGGTACATCGTCCATCT
CTGGGTCGAGGGCGTCTGGGAGCTGATCATGGCCTCGATCCTGGCCTTCCTGATGATCAAGCTCAACGGCATCGACCGTG
AGGTCGTCGAGAAGTGGCTCTATGTCATCATCGGCCTGGCGCTGTTCTCCGGTATCCTCGGCACCGGCCACCATTTCTAC
TGGATCGGCGCTCCCGGCTACTGGCAGTGGATCGGCTCGCTGTTCTCGACGCTGGAGGTCGCGCCGTTCTTCACCATGGT
GATCTTCACGGTGCAGATGACGCTCAAGGCCGGCCGCAAGCATCCGAACCGCGCCGCGCTCCTGTGGTCCGTCGGCTGCT
CCGTGATGGCCTTCCTCGGCGCCGGCGTGTGGGGCTTCCTGCATACGCTGTCGAGCGTAAACTACTACACCCACGGCACC
CAGGTGACCGCCGCGCACGGCCATCTCGCCTTCTTCGGCGCCTATGTGATGCTCAACCTCGCGGTGATGGCCTACGCGGT
GCCGCAGCTGCGGCAGCGGGCGCCGTACAATCAGTGGCTCTCGATCGCGAGCTTCTGGATCATGTGCACGGCGATGATGG
TGATGACCTTCGCGCTGACCTTCGCGGGCATCCTCCAGGTGCATCTGCAGCGCGTGCTCGGCCAGAGCTACATGGACGTG
CAGGACCAGCTCGCGATGTTCTACTGGGTGCGGCTCGGTTCCGGCGTGTTCGTGGCGATCTCGGCGCTGATGTTCGTCTG
GGCCGTGCTGGTGCCCGGCCGCGCGAAGCAGGCCGTGATCCCCGGCGCGCTGCAGCCGGCGGAGTGA

Upstream 100 bases:

>100_bases
CTTCCTGCAATGGACCGACACGATCAAGCGCCAGGATTGGCCGCCGAACAAGGCCGGCTGAGCTCCGCATCCCGCCCTTC
ATCCACGAGAGAGATCCGCG

Downstream 100 bases:

>100_bases
AGGCCGACAGGCGGCGGCCCTGCGGTCGCCGCCTGCATCCCCCGATGTTGGAGAGAAATCATGAAAGCCGCGCTGCACGC
GATCGACACCGTTCCCGCGT

Product: nitric-oxide reductase subunit B

Products: NA

Alternate protein names: NOR large subunit; Nitric oxide reductase cytochrome b subunit [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MRYETQKVAMLYFYGALTLFLAQVLFGLLAGTIYVLPNTLSVLLPFNIVRMIHTNALIVWSLIGFMGATYFLLPEETETE
LYSPLLAKIQFWMFFGAAAAAVVGYLFHYHEGREFLEQPFIIKVGIVVVCLMFLFNVTMTALKGRKTTVTNILLFGMWGV
AIFFLFAFYNPANLAVDKMYWWYIVHLWVEGVWELIMASILAFLMIKLNGIDREVVEKWLYVIIGLALFSGILGTGHHFY
WIGAPGYWQWIGSLFSTLEVAPFFTMVIFTVQMTLKAGRKHPNRAALLWSVGCSVMAFLGAGVWGFLHTLSSVNYYTHGT
QVTAAHGHLAFFGAYVMLNLAVMAYAVPQLRQRAPYNQWLSIASFWIMCTAMMVMTFALTFAGILQVHLQRVLGQSYMDV
QDQLAMFYWVRLGSGVFVAISALMFVWAVLVPGRAKQAVIPGALQPAE

Sequences:

>Translated_448_residues
MRYETQKVAMLYFYGALTLFLAQVLFGLLAGTIYVLPNTLSVLLPFNIVRMIHTNALIVWSLIGFMGATYFLLPEETETE
LYSPLLAKIQFWMFFGAAAAAVVGYLFHYHEGREFLEQPFIIKVGIVVVCLMFLFNVTMTALKGRKTTVTNILLFGMWGV
AIFFLFAFYNPANLAVDKMYWWYIVHLWVEGVWELIMASILAFLMIKLNGIDREVVEKWLYVIIGLALFSGILGTGHHFY
WIGAPGYWQWIGSLFSTLEVAPFFTMVIFTVQMTLKAGRKHPNRAALLWSVGCSVMAFLGAGVWGFLHTLSSVNYYTHGT
QVTAAHGHLAFFGAYVMLNLAVMAYAVPQLRQRAPYNQWLSIASFWIMCTAMMVMTFALTFAGILQVHLQRVLGQSYMDV
QDQLAMFYWVRLGSGVFVAISALMFVWAVLVPGRAKQAVIPGALQPAE
>Mature_448_residues
MRYETQKVAMLYFYGALTLFLAQVLFGLLAGTIYVLPNTLSVLLPFNIVRMIHTNALIVWSLIGFMGATYFLLPEETETE
LYSPLLAKIQFWMFFGAAAAAVVGYLFHYHEGREFLEQPFIIKVGIVVVCLMFLFNVTMTALKGRKTTVTNILLFGMWGV
AIFFLFAFYNPANLAVDKMYWWYIVHLWVEGVWELIMASILAFLMIKLNGIDREVVEKWLYVIIGLALFSGILGTGHHFY
WIGAPGYWQWIGSLFSTLEVAPFFTMVIFTVQMTLKAGRKHPNRAALLWSVGCSVMAFLGAGVWGFLHTLSSVNYYTHGT
QVTAAHGHLAFFGAYVMLNLAVMAYAVPQLRQRAPYNQWLSIASFWIMCTAMMVMTFALTFAGILQVHLQRVLGQSYMDV
QDQLAMFYWVRLGSGVFVAISALMFVWAVLVPGRAKQAVIPGALQPAE

Specific function: Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification). NorB is the catalytic subunit of the enzyme complex. Shows proton pump activity across the membrane in denitrifying bacterial cells. The mononitrogen red

COG id: COG3256

COG function: function code P; Nitric oxide reductase large subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heme-copper respiratory oxidase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000883 [H]

Pfam domain/function: PF00115 COX1 [H]

EC number: =1.7.99.7 [H]

Molecular weight: Translated: 50580; Mature: 50580

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50855 COX1 ; PS00077 COX1_CUB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7508388; 2542222; 9521721 [H]