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The map label for this gene is podJ [H]

Identifier: 146338746

GI number: 146338746

Start: 1796509

End: 1797825

Strand: Direct

Name: podJ [H]

Synonym: BRADO1680

Alternate gene names: 146338746

Gene position: 1796509-1797825 (Clockwise)

Preceding gene: 146338745

Following gene: 146338747

Centisome position: 24.09

GC content: 67.43

Gene sequence:

>1317_bases
ATGATCAAGACGCTCGCCATCTTTTTGACGTTGTTGCTCGCGGCTGCGACTAGCGCCGCGGCCGCCGAGCTCGAGGAGGC
CGTCGCCGCGGCGGCGAATGGACAGCCCGCGGTGGCGTTGCGAAGGCTCCAGCCGCTCGCCGTCAAGGGCGACGCGCGGG
CACAATTCGATGTCGGCTTCATGCAGGCCTTCGGCGTCGGCGTCGCGCGCAACCAGACGGAGGCGCTGGTCTGGTACCGC
AAGGCCGCCGATCAGGGGCTCGCCATCGCCCAGCATTATCTGGGCATCGCCTATTTCAACGGCGAAGGCGCCGCTCGCGA
TCACGGCGAAGCCGCGCGCTGGTTCAACCGCGCCGCCGCCCAAGGCTTCGCCCAATCGCAATACATGCTCGGCCTGATGA
TGCTCGACGGCCGGGTCATGTCCCAGGATGTCGTGCAGGGTTATGCCTGGCTGGTGATGGCCGGCCGCAACGGCGTAAGG
TCGGCCGGCCGCGAGATCCAGCGTATCAAGCTTAGCGACGCACAGCGGCGTCAGGCCGAGCAGATCATCGCGGCGTGGAG
GCCGAAGCCGGAAGCGGCGGATGCCGGCATTGCCAATCCGCGCCCCGAGCAGCTGCTCGGCCTCGACCGGCACTTCGGTG
ATGTCGTCGATCCCACCTCCTGGCCGGCATCGTCGATCGGTGTCGTGACCCAGGCGCAATACAGCACGGGAGGCTCGTGT
ACCGGCACCTTGGTGGCTCCCCGCGTCGTGCTGACGGCGGCGCACTGCGTCTTCAACGGTCTGGCTCAGGTGAGTCCGGG
CAATGTCCATTTTCTGGCGGGCCTGAACAAAGGCACCCCCGCCGCCTCCTCAGTCGCCGACAAGCTGATTGTCGCGCCGG
ACTTCATCCCGACTCAGCACGACCAATGGTCGGCCGACCGTTCGCCGGCGGATTGGGCGCTGATCATCCTGAAGGATGCG
CTGCCGTCCAGGCCGGTGGCGGTAAGGGCCGTGAGCCGCGACGAGCTTGCGGCCGCGACCCAGGCGGGCACGATCTCCGA
GATCGGCTACGGCCAGGAGCGGCGCTATTCGCCGACCGGCCAGCGCAATTGCAGCGCCGGCATGACCAAGGATGCGAAGC
TGCTGACCGTCCATTGCCTCGCCAATTTCGGCTATTCGGGCTCCCCGATTCTCGCCGAGATCAACGGCGAACCGGTCGTG
GTCGGCATCTTCTCGGCGTTCCACGAAGAGACGAGGCTGATGTTCGCCGCGCCTGCAAGCCAGTTCGAGGCGACCGTGCG
GCGCGAGATCGCGGCGCAGAGCGCCTCGGCGCGGTGA

Upstream 100 bases:

>100_bases
CTGAGCAGGCCTGATCGCGCGGTCTGCGCGGTCACGCCGCTGCCGTCTTGATCATGTGTGATGGCGTTCCCATCATCATG
GTCGGGCCTCGGCAGACACA

Downstream 100 bases:

>100_bases
GAGGAGGGCGGGGAGGGAAAGAGTTGGACATGGGCTGGTCAATGTTTGTGCGTGACCGGATAGCGGATTGCCAAGGGAAT
GCGGTGAACCTCGGCCGCGC

Product: hypothetical protein

Products: NA

Alternate protein names: Polar organelle development protein [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MIKTLAIFLTLLLAAATSAAAAELEEAVAAAANGQPAVALRRLQPLAVKGDARAQFDVGFMQAFGVGVARNQTEALVWYR
KAADQGLAIAQHYLGIAYFNGEGAARDHGEAARWFNRAAAQGFAQSQYMLGLMMLDGRVMSQDVVQGYAWLVMAGRNGVR
SAGREIQRIKLSDAQRRQAEQIIAAWRPKPEAADAGIANPRPEQLLGLDRHFGDVVDPTSWPASSIGVVTQAQYSTGGSC
TGTLVAPRVVLTAAHCVFNGLAQVSPGNVHFLAGLNKGTPAASSVADKLIVAPDFIPTQHDQWSADRSPADWALIILKDA
LPSRPVAVRAVSRDELAAATQAGTISEIGYGQERRYSPTGQRNCSAGMTKDAKLLTVHCLANFGYSGSPILAEINGEPVV
VGIFSAFHEETRLMFAAPASQFEATVRREIAAQSASAR

Sequences:

>Translated_438_residues
MIKTLAIFLTLLLAAATSAAAAELEEAVAAAANGQPAVALRRLQPLAVKGDARAQFDVGFMQAFGVGVARNQTEALVWYR
KAADQGLAIAQHYLGIAYFNGEGAARDHGEAARWFNRAAAQGFAQSQYMLGLMMLDGRVMSQDVVQGYAWLVMAGRNGVR
SAGREIQRIKLSDAQRRQAEQIIAAWRPKPEAADAGIANPRPEQLLGLDRHFGDVVDPTSWPASSIGVVTQAQYSTGGSC
TGTLVAPRVVLTAAHCVFNGLAQVSPGNVHFLAGLNKGTPAASSVADKLIVAPDFIPTQHDQWSADRSPADWALIILKDA
LPSRPVAVRAVSRDELAAATQAGTISEIGYGQERRYSPTGQRNCSAGMTKDAKLLTVHCLANFGYSGSPILAEINGEPVV
VGIFSAFHEETRLMFAAPASQFEATVRREIAAQSASAR
>Mature_438_residues
MIKTLAIFLTLLLAAATSAAAAELEEAVAAAANGQPAVALRRLQPLAVKGDARAQFDVGFMQAFGVGVARNQTEALVWYR
KAADQGLAIAQHYLGIAYFNGEGAARDHGEAARWFNRAAAQGFAQSQYMLGLMMLDGRVMSQDVVQGYAWLVMAGRNGVR
SAGREIQRIKLSDAQRRQAEQIIAAWRPKPEAADAGIANPRPEQLLGLDRHFGDVVDPTSWPASSIGVVTQAQYSTGGSC
TGTLVAPRVVLTAAHCVFNGLAQVSPGNVHFLAGLNKGTPAASSVADKLIVAPDFIPTQHDQWSADRSPADWALIILKDA
LPSRPVAVRAVSRDELAAATQAGTISEIGYGQERRYSPTGQRNCSAGMTKDAKLLTVHCLANFGYSGSPILAEINGEPVV
VGIFSAFHEETRLMFAAPASQFEATVRREIAAQSASAR

Specific function: PodJL provides the positional information for the localization of several polar organelles (pili, adhesive holdfast and chemotactic apparatus) by recruiting structural (CpaE) and regulatory (PleC) proteins to a specific cell pole [H]

COG id: COG0790

COG function: function code R; FOG: TPR repeat, SEL1 subfamily

Gene ontology:

Cell location: Membrane; Single-pass membrane protein. Note=Both PodJL and podJS localize to the swarmer pole at different stages of the cell division [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 Sel1-like repeats [H]

Homologues:

Organism=Escherichia coli, GI87081769, Length=128, Percent_Identity=33.59375, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1786867, Length=119, Percent_Identity=31.0924369747899, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002477
- InterPro:   IPR006597
- InterPro:   IPR011990 [H]

Pfam domain/function: PF01471 PG_binding_1; PF08238 Sel1 [H]

EC number: NA

Molecular weight: Translated: 46521; Mature: 46521

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTLAIFLTLLLAAATSAAAAELEEAVAAAANGQPAVALRRLQPLAVKGDARAQFDVGF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCCEEHHHH
MQAFGVGVARNQTEALVWYRKAADQGLAIAQHYLGIAYFNGEGAARDHGEAARWFNRAAA
HHHHCCCCCCCCCHHHEEEHHHCCCCHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHH
QGFAQSQYMLGLMMLDGRVMSQDVVQGYAWLVMAGRNGVRSAGREIQRIKLSDAQRRQAE
HHHHHHHHHHHHHEECCCHHHHHHHCCEEEEEEECCCCHHHHHHHHHEECCCHHHHHHHH
QIIAAWRPKPEAADAGIANPRPEQLLGLDRHFGDVVDPTSWPASSIGVVTQAQYSTGGSC
HHHHHCCCCCCCCCCCCCCCCHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCC
TGTLVAPRVVLTAAHCVFNGLAQVSPGNVHFLAGLNKGTPAASSVADKLIVAPDFIPTQH
EEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHEEECCCCCCCCC
DQWSADRSPADWALIILKDALPSRPVAVRAVSRDELAAATQAGTISEIGYGQERRYSPTG
CCCCCCCCCCCEEEEEEECCCCCCCEEEEEECHHHHHHHHCCCCHHHCCCCCCCCCCCCC
QRNCSAGMTKDAKLLTVHCLANFGYSGSPILAEINGEPVVVGIFSAFHEETRLMFAAPAS
CCCCCCCCCCCCCEEEEEEHHHCCCCCCCEEEEECCCEEEEEHHHHHHHHCEEEEECCHH
QFEATVRREIAAQSASAR
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIKTLAIFLTLLLAAATSAAAAELEEAVAAAANGQPAVALRRLQPLAVKGDARAQFDVGF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCCEEHHHH
MQAFGVGVARNQTEALVWYRKAADQGLAIAQHYLGIAYFNGEGAARDHGEAARWFNRAAA
HHHHCCCCCCCCCHHHEEEHHHCCCCHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHH
QGFAQSQYMLGLMMLDGRVMSQDVVQGYAWLVMAGRNGVRSAGREIQRIKLSDAQRRQAE
HHHHHHHHHHHHHEECCCHHHHHHHCCEEEEEEECCCCHHHHHHHHHEECCCHHHHHHHH
QIIAAWRPKPEAADAGIANPRPEQLLGLDRHFGDVVDPTSWPASSIGVVTQAQYSTGGSC
HHHHHCCCCCCCCCCCCCCCCHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCC
TGTLVAPRVVLTAAHCVFNGLAQVSPGNVHFLAGLNKGTPAASSVADKLIVAPDFIPTQH
EEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHEEECCCCCCCCC
DQWSADRSPADWALIILKDALPSRPVAVRAVSRDELAAATQAGTISEIGYGQERRYSPTG
CCCCCCCCCCCEEEEEEECCCCCCCEEEEEECHHHHHHHHCCCCHHHCCCCCCCCCCCCC
QRNCSAGMTKDAKLLTVHCLANFGYSGSPILAEINGEPVVVGIFSAFHEETRLMFAAPAS
CCCCCCCCCCCCCEEEEEEHHHCCCCCCCEEEEECCCEEEEEHHHHHHHHCEEEEECCHH
QFEATVRREIAAQSASAR
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12370432 [H]