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The map label for this gene is ooxB [H]

Identifier: 146338624

GI number: 146338624

Start: 1660380

End: 1661528

Strand: Direct

Name: ooxB [H]

Synonym: BRADO1552

Alternate gene names: 146338624

Gene position: 1660380-1661528 (Clockwise)

Preceding gene: 146338623

Following gene: 146338625

Centisome position: 22.27

GC content: 69.19

Gene sequence:

>1149_bases
ATGACCCGGCGCATTGACTGCGATGTCGCGATCATCGGCGGCGGGCTCGTCGGCAGCTCCGCGGCGTTGGCGCTGCGCGG
CATGGGTTTCTCGGTTACCTTGCTCGACAAGGGGTTCTGCGGCGCGCAGGCGAGCGGCGTGAACTATGGCGGCGTGCGCC
GTCAGGGCCGTCCGCCCGAGCAATTGCCGCTGTCGCAGCGCTCGCACGCGATCTGGCCGCGGCTGAAACAACTCATCGGC
ATCGACGGTGAATTCCTGCGCTCCGGCCATCTCAAGCTGGCGCGGACGCCCGAGGATATGGCGAGCCTGGAGCGCTATGC
CGCCGAGGTCGCGCCGTTCGGACTCGATCTCGAACTGGTCGGCCACAATCAGCTCAGCGAGCGCTTCGGCATCGCCGGCG
GCGTCGTCGGCGGCTCGTTTTGTGCTGGCGACGGCCATGCCAATCCGCGGCTGGTTTCGACGGCGTTTGCCGCGGCGGCG
CGCCGCGCGGGTGCGGAGGTGCTGGAGAACACCAGGGTCATTGGTGCCACCACCGCGAACGGCGGCTTTGCGCTGGAGGC
CGAGGGCGTCGCCATCACCGCGCGCACGCTGATCAACAGCGCGGGCGCCTGGGCCGACAGCTTTGCGGCTGCATTCAACG
AGCCGGTGCCGCTGGAGCGCACCTATCCGTCGATGATCGTGACCGAGCCGCTTGATCCGTTCCTGTCCGTCAACATCGGC
ATCGAAGGCGGCGGCATCTATGCGCGCCAGGTGACGCGCGGCAATGTCGTGGTCGGCGGCGAGCGCGCCGCGCCGCTCGC
GGATCCGGATTATTCGCGCCCGCGCAGCGACGGCGCCCTCGCGATCATGCGGCGCGCCAGCGAGCTGTTCGAACCACTGC
GCCACGCGCAGGCGATCAGGTTCTGGAGCGGCACCGAGGCGACGATGTCCGACCGCAATCCGGTGATCGGCCCGAGCGCG
ACGACGCCGGGACTCATCCACGCCTTCGGCTTCTCCGGCGCCGGCTTCCAGATCGCGCCGGGCGTAGGCGAGGTCCTCGC
CGAACTGGTGCGCGATGGCCGGACCGCGACGCCGATCGACGCATTCACGATCAGCCGTTTCTCACCCGCATCGCAGCCGG
GCGATGCTCCCTCCCTGAAGACAGGATAA

Upstream 100 bases:

>100_bases
CAGGCGAACCGCAACATCGGCGCGGTTGGCCGGCTGCGCGCGCAGGCCCCGATCAAGCCGATTCCATTGGCCGTCAGCCT
GTCCACCGACGAGAAGCAGC

Downstream 100 bases:

>100_bases
GGAGACCTCGCCATGATCCATGCCCGTGCCGCGCTCGGCTCAGCCTTCGCGCTGATGCTCGCGACATCCGTCGCGCACGC
CGAGACCAAGACGCTCTATG

Product: oxidoreductase

Products: glycine; formaldehyde; H2O2

Alternate protein names: Octopine oxidase subunit B [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTRRIDCDVAIIGGGLVGSSAALALRGMGFSVTLLDKGFCGAQASGVNYGGVRRQGRPPEQLPLSQRSHAIWPRLKQLIG
IDGEFLRSGHLKLARTPEDMASLERYAAEVAPFGLDLELVGHNQLSERFGIAGGVVGGSFCAGDGHANPRLVSTAFAAAA
RRAGAEVLENTRVIGATTANGGFALEAEGVAITARTLINSAGAWADSFAAAFNEPVPLERTYPSMIVTEPLDPFLSVNIG
IEGGGIYARQVTRGNVVVGGERAAPLADPDYSRPRSDGALAIMRRASELFEPLRHAQAIRFWSGTEATMSDRNPVIGPSA
TTPGLIHAFGFSGAGFQIAPGVGEVLAELVRDGRTATPIDAFTISRFSPASQPGDAPSLKTG

Sequences:

>Translated_382_residues
MTRRIDCDVAIIGGGLVGSSAALALRGMGFSVTLLDKGFCGAQASGVNYGGVRRQGRPPEQLPLSQRSHAIWPRLKQLIG
IDGEFLRSGHLKLARTPEDMASLERYAAEVAPFGLDLELVGHNQLSERFGIAGGVVGGSFCAGDGHANPRLVSTAFAAAA
RRAGAEVLENTRVIGATTANGGFALEAEGVAITARTLINSAGAWADSFAAAFNEPVPLERTYPSMIVTEPLDPFLSVNIG
IEGGGIYARQVTRGNVVVGGERAAPLADPDYSRPRSDGALAIMRRASELFEPLRHAQAIRFWSGTEATMSDRNPVIGPSA
TTPGLIHAFGFSGAGFQIAPGVGEVLAELVRDGRTATPIDAFTISRFSPASQPGDAPSLKTG
>Mature_381_residues
TRRIDCDVAIIGGGLVGSSAALALRGMGFSVTLLDKGFCGAQASGVNYGGVRRQGRPPEQLPLSQRSHAIWPRLKQLIGI
DGEFLRSGHLKLARTPEDMASLERYAAEVAPFGLDLELVGHNQLSERFGIAGGVVGGSFCAGDGHANPRLVSTAFAAAAR
RAGAEVLENTRVIGATTANGGFALEAEGVAITARTLINSAGAWADSFAAAFNEPVPLERTYPSMIVTEPLDPFLSVNIGI
EGGGIYARQVTRGNVVVGGERAAPLADPDYSRPRSDGALAIMRRASELFEPLRHAQAIRFWSGTEATMSDRNPVIGPSAT
TPGLIHAFGFSGAGFQIAPGVGEVLAELVRDGRTATPIDAFTISRFSPASQPGDAPSLKTG

Specific function: Oxidative cleavage of octopine into L-arginine and pyruvate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI194306651, Length=379, Percent_Identity=24.2744063324538, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI197927446, Length=383, Percent_Identity=22.4543080939948, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI21361378, Length=383, Percent_Identity=22.4543080939948, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1787559, Length=394, Percent_Identity=25.6345177664975, Blast_Score=63, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28571104, Length=384, Percent_Identity=24.7395833333333, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI20130091, Length=382, Percent_Identity=24.0837696335079, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.5.3.1

Molecular weight: Translated: 39848; Mature: 39716

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRIDCDVAIIGGGLVGSSAALALRGMGFSVTLLDKGFCGAQASGVNYGGVRRQGRPPE
CCCEEECCEEEEECCCCCCCHHHEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCH
QLPLSQRSHAIWPRLKQLIGIDGEFLRSGHLKLARTPEDMASLERYAAEVAPFGLDLELV
HCCCCCCCCHHHHHHHHHHCCCHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEE
GHNQLSERFGIAGGVVGGSFCAGDGHANPRLVSTAFAAAARRAGAEVLENTRVIGATTAN
CCCHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
GGFALEAEGVAITARTLINSAGAWADSFAAAFNEPVPLERTYPSMIVTEPLDPFLSVNIG
CCEEEEECCEEEEHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEEEEE
IEGGGIYARQVTRGNVVVGGERAAPLADPDYSRPRSDGALAIMRRASELFEPLRHAQAIR
EECCCEEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEE
FWSGTEATMSDRNPVIGPSATTPGLIHAFGFSGAGFQIAPGVGEVLAELVRDGRTATPID
EECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEECCCHHHHHHHHHHCCCCCCCCC
AFTISRFSPASQPGDAPSLKTG
CEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRRIDCDVAIIGGGLVGSSAALALRGMGFSVTLLDKGFCGAQASGVNYGGVRRQGRPPE
CCEEECCEEEEECCCCCCCHHHEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCH
QLPLSQRSHAIWPRLKQLIGIDGEFLRSGHLKLARTPEDMASLERYAAEVAPFGLDLELV
HCCCCCCCCHHHHHHHHHHCCCHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEE
GHNQLSERFGIAGGVVGGSFCAGDGHANPRLVSTAFAAAARRAGAEVLENTRVIGATTAN
CCCHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
GGFALEAEGVAITARTLINSAGAWADSFAAAFNEPVPLERTYPSMIVTEPLDPFLSVNIG
CCEEEEECCEEEEHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEEEEE
IEGGGIYARQVTRGNVVVGGERAAPLADPDYSRPRSDGALAIMRRASELFEPLRHAQAIR
EECCCEEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEE
FWSGTEATMSDRNPVIGPSATTPGLIHAFGFSGAGFQIAPGVGEVLAELVRDGRTATPID
EECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEECCCHHHHHHHHHHCCCCCCCCC
AFTISRFSPASQPGDAPSLKTG
CEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sarcosine; H2O; O2

Specific reaction: sarcosine + H2O + O2 = glycine + formaldehyde + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045881 [H]