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The map label for this gene is kpnIM [H]

Identifier: 146338458

GI number: 146338458

Start: 1460106

End: 1462307

Strand: Direct

Name: kpnIM [H]

Synonym: BRADO1375

Alternate gene names: 146338458

Gene position: 1460106-1462307 (Clockwise)

Preceding gene: 146338457

Following gene: 146338459

Centisome position: 19.58

GC content: 50.23

Gene sequence:

>2202_bases
ATGGCAAAGATTGAAGATTTGATTGCAAGAATCCCGGACGCCGGCCTCCGTATCGCGATAGGCAATGAAGTTAGGGAACT
GAAGAAGGCTAAGAAATTCGGACTGGTTTTCGAGAATCACTTGCCTGAGACGGTTCAACTCCCAAACCTGCCTGTCAGAG
TCGGGGAAACGGTTGCCCGCAAACGGGATTCAGGGGAAATCTTGTGGATCGTGAAATCGATTCAAAAGGGGGTCGCAACA
CTTAGGCCAATGATTGAAGCGCCGACTCAAAATGAAATGCCCGTTGATGTCGGTATTGACGAGCTGGTTGTCGTCAGGCG
CTTCGGTGATCCAATCTATCCCACCTTGGTGTCAGTTGACAGGGTGAATCGAGGCGGACCGCAGAAGCCTTGGCACACGC
TCATTAAGGCGGACAATTTTCACGCCCTGCAGCTTTTGCTTTTCTGCTACCCGTCATCCGTGGACGTCATCTATATCGAC
CCGCCTTATAACTCCGGTGCGCGCGACTGGAAGTACAACAATGACTATGTTGATAAGACCGATACTTTCCGGCACTCGAA
GTGGCTATCAATGATGAAGAAGAGGCTTCTAATCGCGAAGCATCTCCTCAAGCCCGATGGGGTTCTCATCGTAACCATAG
ATGAGAACGAGCTTCACCACTTAGGCCTGCTGCTGGAGGATGTATTTGAATCTTACCTTCGTCACACTGTAACGATCGTT
ATAAACCCGAAGGGTGCCGGCAAGAGAAATTTTGCCCGAACAGAAGAGCACGCACTCTTTTGTGTCCCAAAAACGGGGCA
GGCAATCGTCAACGCGAATATGCTTCGTGATCTGTCAAGAGTCGGGCTCTCTACCGAGATGGTTCAAGAAGAGTTCGACG
AAGAGCTTGATGACGAGCCAGCCGAGCTTGCAGACGAAACCTCCGACGATGGATCTTTATTTGAACAGCAACTTTCCGAC
GATGTCGGATTAGACGAGCTGCCATTTCCGCCGGACGAACTAGATCAATGGGAGCTTCGACATGCGCGTCGACGCGGCAA
TGAATCTAGCTATCGGCATCAGCGTAAGAATCAGTTCTATCCAATCTACATTGATGAAAAGAGGCGGGTGGTCGAAGAGA
TTGGTGAGCCGCTTCTTCCTCTCGAAGCTCAACCGAGCTTCAAGAAAAAAAATGGTCTGACGCCCGTTTGGCCAATTGAC
AAAGAGGGGAATCATCGCTGCTGGCGATTCATTGCGTCTTCCATGAAGAAAATTCATGAAGAGAAGCGGCTGGTCGTAGG
CAGGCAAGACCCAACCACAAAAGGCTGGACCCTCAACTATTGGATTCCGAAGAGCAAAACTAAAAATGTCAAAACGGTCT
GGTGGCACGCTCGCCACGACGCAGGCACTCACGGGACCAGTATGCTCCACAAGATTCTCGGGCGACGCGATGCTTTTCCT
TTTCCAAAATCTCTTTACGCTACCCGAGATGCGCTCCTGACTGTAATTGGAAGTCGCCCTAATGCTCTCGTTCTAGATTT
TTTCGCTGGTTCAGGTACTACTTTGCACGCTACAGCTCTACTGAATGCCCAGCTTGGTGGAAGTCGACGTTGCATTCTCG
TATCTAATAATGAGCCGGGTGCTACCGTCGCTGGTAAGCTTCGCCGCAAACAAATATATCCCGGAGACGCGGATTACGAA
GCTGCGGGTATCTGTGAGAGTGTAACTTGGCCGCGGTGTAAGTTTGTCATCAATGGTAAACGCGATGACGGAACAGAGCT
TGCTGGGACTTACCTAGACATTGAGGGGCACGAGAAAAATCTGCGTTGGTCGGACGGCTTTGAAGAGAATATCGAGTATT
TCAATCTCGACTTTCTCGAGCCAGATGAGGTAGCCCGAGGAGATGCTTTTCGATCGATTCTTCCAATTCTTTGGTTGATT
GCTGGGGCGTCGGGCGAGCGTGAAGACTCTAAGGGCACGTCTGCCTGGCACCTTCCGGCGCGCTCTCCCTATGCAGTTTT
AATACGCGAAAAGGAGTTCTTGCACTTCAAAGAACGGTTGGCAGGGCGGAGTGACGTTGATTGGGTTTTCTTGGTGACTG
ATAGCGAAGAAAACTTTGCCCTCATGCGTCGTGCTCTCGGTAAGAAATACAAATGCTATCAGCTCTACAAGCAGTATCTT
GAGAATTTTCGGATCAACGTGGCCGAAGTACTGATGGATTAG

Upstream 100 bases:

>100_bases
CGGAAGTTTACTGGCAAAATAGACAACAACACCGAGCCTGACATTGCTCAGGTTTGCCGACCGAACTAAAAACTGACCGA
TACGCAAAGCGAAAGTCGTT

Downstream 100 bases:

>100_bases
CGCGCATGAAAGTTGAGCTTAAGACCTTCCAAACTGATGCGGCGCTTAGCGTTATCGAAGAGATTGACGAGGCGAGGGTA
GCGGTCGGCAAGGGTAAGCT

Product: putative adenine-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.KpnI; Adenine-specific methyltransferase KpnI [H]

Number of amino acids: Translated: 733; Mature: 732

Protein sequence:

>733_residues
MAKIEDLIARIPDAGLRIAIGNEVRELKKAKKFGLVFENHLPETVQLPNLPVRVGETVARKRDSGEILWIVKSIQKGVAT
LRPMIEAPTQNEMPVDVGIDELVVVRRFGDPIYPTLVSVDRVNRGGPQKPWHTLIKADNFHALQLLLFCYPSSVDVIYID
PPYNSGARDWKYNNDYVDKTDTFRHSKWLSMMKKRLLIAKHLLKPDGVLIVTIDENELHHLGLLLEDVFESYLRHTVTIV
INPKGAGKRNFARTEEHALFCVPKTGQAIVNANMLRDLSRVGLSTEMVQEEFDEELDDEPAELADETSDDGSLFEQQLSD
DVGLDELPFPPDELDQWELRHARRRGNESSYRHQRKNQFYPIYIDEKRRVVEEIGEPLLPLEAQPSFKKKNGLTPVWPID
KEGNHRCWRFIASSMKKIHEEKRLVVGRQDPTTKGWTLNYWIPKSKTKNVKTVWWHARHDAGTHGTSMLHKILGRRDAFP
FPKSLYATRDALLTVIGSRPNALVLDFFAGSGTTLHATALLNAQLGGSRRCILVSNNEPGATVAGKLRRKQIYPGDADYE
AAGICESVTWPRCKFVINGKRDDGTELAGTYLDIEGHEKNLRWSDGFEENIEYFNLDFLEPDEVARGDAFRSILPILWLI
AGASGEREDSKGTSAWHLPARSPYAVLIREKEFLHFKERLAGRSDVDWVFLVTDSEENFALMRRALGKKYKCYQLYKQYL
ENFRINVAEVLMD

Sequences:

>Translated_733_residues
MAKIEDLIARIPDAGLRIAIGNEVRELKKAKKFGLVFENHLPETVQLPNLPVRVGETVARKRDSGEILWIVKSIQKGVAT
LRPMIEAPTQNEMPVDVGIDELVVVRRFGDPIYPTLVSVDRVNRGGPQKPWHTLIKADNFHALQLLLFCYPSSVDVIYID
PPYNSGARDWKYNNDYVDKTDTFRHSKWLSMMKKRLLIAKHLLKPDGVLIVTIDENELHHLGLLLEDVFESYLRHTVTIV
INPKGAGKRNFARTEEHALFCVPKTGQAIVNANMLRDLSRVGLSTEMVQEEFDEELDDEPAELADETSDDGSLFEQQLSD
DVGLDELPFPPDELDQWELRHARRRGNESSYRHQRKNQFYPIYIDEKRRVVEEIGEPLLPLEAQPSFKKKNGLTPVWPID
KEGNHRCWRFIASSMKKIHEEKRLVVGRQDPTTKGWTLNYWIPKSKTKNVKTVWWHARHDAGTHGTSMLHKILGRRDAFP
FPKSLYATRDALLTVIGSRPNALVLDFFAGSGTTLHATALLNAQLGGSRRCILVSNNEPGATVAGKLRRKQIYPGDADYE
AAGICESVTWPRCKFVINGKRDDGTELAGTYLDIEGHEKNLRWSDGFEENIEYFNLDFLEPDEVARGDAFRSILPILWLI
AGASGEREDSKGTSAWHLPARSPYAVLIREKEFLHFKERLAGRSDVDWVFLVTDSEENFALMRRALGKKYKCYQLYKQYL
ENFRINVAEVLMD
>Mature_732_residues
AKIEDLIARIPDAGLRIAIGNEVRELKKAKKFGLVFENHLPETVQLPNLPVRVGETVARKRDSGEILWIVKSIQKGVATL
RPMIEAPTQNEMPVDVGIDELVVVRRFGDPIYPTLVSVDRVNRGGPQKPWHTLIKADNFHALQLLLFCYPSSVDVIYIDP
PYNSGARDWKYNNDYVDKTDTFRHSKWLSMMKKRLLIAKHLLKPDGVLIVTIDENELHHLGLLLEDVFESYLRHTVTIVI
NPKGAGKRNFARTEEHALFCVPKTGQAIVNANMLRDLSRVGLSTEMVQEEFDEELDDEPAELADETSDDGSLFEQQLSDD
VGLDELPFPPDELDQWELRHARRRGNESSYRHQRKNQFYPIYIDEKRRVVEEIGEPLLPLEAQPSFKKKNGLTPVWPIDK
EGNHRCWRFIASSMKKIHEEKRLVVGRQDPTTKGWTLNYWIPKSKTKNVKTVWWHARHDAGTHGTSMLHKILGRRDAFPF
PKSLYATRDALLTVIGSRPNALVLDFFAGSGTTLHATALLNAQLGGSRRCILVSNNEPGATVAGKLRRKQIYPGDADYEA
AGICESVTWPRCKFVINGKRDDGTELAGTYLDIEGHEKNLRWSDGFEENIEYFNLDFLEPDEVARGDAFRSILPILWLIA
GASGEREDSKGTSAWHLPARSPYAVLIREKEFLHFKERLAGRSDVDWVFLVTDSEENFALMRRALGKKYKCYQLYKQYLE
NFRINVAEVLMD

Specific function: This methylase recognizes the double-stranded sequence GGTACC, causes specific methylation on A-4 on both strands, and protects the DNA from cleavage by the KpnI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 83717; Mature: 83586

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIEDLIARIPDAGLRIAIGNEVRELKKAKKFGLVFENHLPETVQLPNLPVRVGETVAR
CCHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCEECCCCCCEEECCCCCCHHCHHHHH
KRDSGEILWIVKSIQKGVATLRPMIEAPTQNEMPVDVGIDELVVVRRFGDPIYPTLVSVD
CCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
RVNRGGPQKPWHTLIKADNFHALQLLLFCYPSSVDVIYIDPPYNSGARDWKYNNDYVDKT
HCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC
DTFRHSKWLSMMKKRLLIAKHLLKPDGVLIVTIDENELHHLGLLLEDVFESYLRHTVTIV
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEE
INPKGAGKRNFARTEEHALFCVPKTGQAIVNANMLRDLSRVGLSTEMVQEEFDEELDDEP
ECCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCH
AELADETSDDGSLFEQQLSDDVGLDELPFPPDELDQWELRHARRRGNESSYRHQRKNQFY
HHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCEE
PIYIDEKRRVVEEIGEPLLPLEAQPSFKKKNGLTPVWPIDKEGNHRCWRFIASSMKKIHE
EEEECCHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EKRLVVGRQDPTTKGWTLNYWIPKSKTKNVKTVWWHARHDAGTHGTSMLHKILGRRDAFP
HHHEEECCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCC
FPKSLYATRDALLTVIGSRPNALVLDFFAGSGTTLHATALLNAQLGGSRRCILVSNNEPG
CCHHHHHHHHHHHHEECCCCCEEEEEEECCCCCEEEEEEEEEECCCCCEEEEEEECCCCC
ATVAGKLRRKQIYPGDADYEAAGICESVTWPRCKFVINGKRDDGTELAGTYLDIEGHEKN
CHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCC
LRWSDGFEENIEYFNLDFLEPDEVARGDAFRSILPILWLIAGASGEREDSKGTSAWHLPA
CCCCCCHHHCCCEEEECCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCC
RSPYAVLIREKEFLHFKERLAGRSDVDWVFLVTDSEENFALMRRALGKKYKCYQLYKQYL
CCCEEEEEECHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHH
ENFRINVAEVLMD
HHCCCCHHHHHCC
>Mature Secondary Structure 
AKIEDLIARIPDAGLRIAIGNEVRELKKAKKFGLVFENHLPETVQLPNLPVRVGETVAR
CHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCEECCCCCCEEECCCCCCHHCHHHHH
KRDSGEILWIVKSIQKGVATLRPMIEAPTQNEMPVDVGIDELVVVRRFGDPIYPTLVSVD
CCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
RVNRGGPQKPWHTLIKADNFHALQLLLFCYPSSVDVIYIDPPYNSGARDWKYNNDYVDKT
HCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC
DTFRHSKWLSMMKKRLLIAKHLLKPDGVLIVTIDENELHHLGLLLEDVFESYLRHTVTIV
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEE
INPKGAGKRNFARTEEHALFCVPKTGQAIVNANMLRDLSRVGLSTEMVQEEFDEELDDEP
ECCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCH
AELADETSDDGSLFEQQLSDDVGLDELPFPPDELDQWELRHARRRGNESSYRHQRKNQFY
HHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCEE
PIYIDEKRRVVEEIGEPLLPLEAQPSFKKKNGLTPVWPIDKEGNHRCWRFIASSMKKIHE
EEEECCHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EKRLVVGRQDPTTKGWTLNYWIPKSKTKNVKTVWWHARHDAGTHGTSMLHKILGRRDAFP
HHHEEECCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCC
FPKSLYATRDALLTVIGSRPNALVLDFFAGSGTTLHATALLNAQLGGSRRCILVSNNEPG
CCHHHHHHHHHHHHEECCCCCEEEEEEECCCCCEEEEEEEEEECCCCCEEEEEEECCCCC
ATVAGKLRRKQIYPGDADYEAAGICESVTWPRCKFVINGKRDDGTELAGTYLDIEGHEKN
CHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCC
LRWSDGFEENIEYFNLDFLEPDEVARGDAFRSILPILWLIAGASGEREDSKGTSAWHLPA
CCCCCCHHHCCCEEEECCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCC
RSPYAVLIREKEFLHFKERLAGRSDVDWVFLVTDSEENFALMRRALGKKYKCYQLYKQYL
CCCEEEEEECHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHH
ENFRINVAEVLMD
HHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1754388 [H]