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The map label for this gene is dnaG [H]

Identifier: 146338437

GI number: 146338437

Start: 1438075

End: 1440081

Strand: Direct

Name: dnaG [H]

Synonym: BRADO1353

Alternate gene names: 146338437

Gene position: 1438075-1440081 (Clockwise)

Preceding gene: 146338436

Following gene: 146338438

Centisome position: 19.29

GC content: 67.91

Gene sequence:

>2007_bases
ATGGCCCGCTTCACGCCCGAATTCCTCGACGAGCTGCGCGCCCGGCTTCCGGTGTCGGATGTCGTCGGCAAGCGCGTGAA
GCTCAAGAAGGCGGGGCGCGAGTGGAAGGGGCTGTCGCCGTTCCAGCAGGAGAAGACGCCGTCCTTCTACGTCAACGACC
AGAAGGGTTTCTATCACGACTTCTCCTCCGGCAAGCACGGCAACATCTTCGACTTCATCATGGAGACCGAGGGCTGCTCG
TTCCCCGAGGCCGTGGAGCGGCTGGCAGGACTCGCCGGCCTGGCGCTGCCGGTGGCGACGCCGGATGCGGCGCGCGAGGC
GCGGCAACGCAAGACGCTGTATGACGTGATGGAGCTGGCGGCGAAGTTCTTCGCCGACACGCTGGCCTCCAGGCACGGCG
CAAGAGCCCGCGGCTATCTCGCCGATCGCGCGATCTTGCCGCAGACGCAGCTTCAGTTCCGGCTCGGCTACGCGCCGCCG
GATCGCTTCGCGCTGAAGGAACATCTCGGCGCGCAGGGCGTGCCGGTCAACGACATGGTCGAGGCGGGGCTGCTCATTGG
CGGCGAGGACATTCCCGTGCCGTACGACCGCTTCAAAGACCGCGTGATGTTCCCGATCACCGACCTCCGCGGCCGCGTCA
TCGCCTTCGGCGGCCGGGCGATGCAGAAGGACGTGCCGGCGAAATATTTGAACTCGCCGGAGACGCCGCTCTTCCACAAG
GGCGACAATCTCTACAACCTGGCCAGCGCCCGCCAGGCTGCGCACAACGGCGCGCCGATGATCGTGGTCGAGGGCTATGT
CGACGTCATCGCGCTGGTCGCGGCGGGCTTTGCCGGCGCGGTGGCGCCGCTCGGCACCGCGCTGACCGAGAGCCAGCTGT
CCTTGATCTGGAAGATGGTCGACGAGCCGATCCTGTGCTTCGACGGCGACCGCGCCGGCCAGAAGGCGGCCCATCGCGCC
GCCGATATCGCGCTGCCGCTGCTCAAGCCGGGCAAGAGCCTGCGCTTCGCGCTGCTGCCCGAGGGCCAGGACCCGGACGA
TCTCGTCCGCTCCGGCGGCCGCGGCGCGGTCGAGGAGGTGCTGAAAATCGCAAAGCCGCTCGCCGAGGTGATCTGGTCGC
GCGAGATCGAGGGCGGCAGCTTCAACACTCCCGAGCGGCGCGCCGCGCTGGAGGCGCGCATCGGCCAGCTCACCGGCGCC
ATCGCCGACGAGGTGGTACGCCGTTATTACAAGCAGGACCTCAGCAACCGCCTTTACCAAGCCTTCGCCCCTGACGGCGG
CCGCAGGTTCAGCCGCGGGTTCGGTGCCGAATCAGGACGCCGGATTGCCCCGCGCGGTCCCGGTTCCGGACAGCAGGGGC
GGTTATCCACAGGGGGCGGCCGCCGCGGCGGTCCGCCATCCACAGGCCCGGCGATGGCCCGCGGCCCCTATATGGCGGCC
AGCCCGCAGCTTGCCATGAGTCCGATCATGCGCGGCCAGCGCTCGGCGATCTCGCGCCGCGAGGCGCTGATCCTGCAATG
CCTGATCAACCACCCCTGGCTGCTGCACGACCATCTCGAGGAGATCGCAGCGCTCGAGCTCGCCCACCCCGAGGCGCACA
AGCTCCGCGCCGCCATCATCGCCGCCTTCGCCCACGACCACCACCATTCGCCGGATCCCAACGAGCAGGCCGAGAAGATG
CGCGCGGACCTCGAAAAGGGCGGCTTTTCCGAGCTTCTTCAACGGGTTGAGCGGGCGATCACGACGCCTGCGGTGTGGGC
CGCCAAGCCCGGCGCGGCGCGCGATGACGTTCTTGCGACATGGCACCAGCTCGTCGCCTTGCATCGGCAATGGCACTCCT
TACTTAGGGAATTGAAGGATGCCGAGCTTGCGTTGGGAGAGGACCCGAGCGAGCCGAACATGGGCTGGTTGAGGGACGTG
AAGGCCAGGATGGCCGAGATGGATGGCACCGAAGCGCTGATCGAAGGCTTCGGGGAACTGTCCGGACGTACCAACCGAAA
CGTGTAG

Upstream 100 bases:

>100_bases
CGCGGCCTGTGAATAGCCCGGACAAGGCCGAGGAAGTTCACATTTCGTACACGGCTGCCTATATGCGTAAGTCCAGCAGA
ACAGGACCGCCACACCGCCC

Downstream 100 bases:

>100_bases
ATCTGCGGCCCGGCGCCCTGTCGTTGACGAGCACATTCCGGCCCGGCTGGTGTCAGCGGTCACGGGCCCGCGCGAAAAGA
CTCGCCAAATCCATGGTTTG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MARFTPEFLDELRARLPVSDVVGKRVKLKKAGREWKGLSPFQQEKTPSFYVNDQKGFYHDFSSGKHGNIFDFIMETEGCS
FPEAVERLAGLAGLALPVATPDAAREARQRKTLYDVMELAAKFFADTLASRHGARARGYLADRAILPQTQLQFRLGYAPP
DRFALKEHLGAQGVPVNDMVEAGLLIGGEDIPVPYDRFKDRVMFPITDLRGRVIAFGGRAMQKDVPAKYLNSPETPLFHK
GDNLYNLASARQAAHNGAPMIVVEGYVDVIALVAAGFAGAVAPLGTALTESQLSLIWKMVDEPILCFDGDRAGQKAAHRA
ADIALPLLKPGKSLRFALLPEGQDPDDLVRSGGRGAVEEVLKIAKPLAEVIWSREIEGGSFNTPERRAALEARIGQLTGA
IADEVVRRYYKQDLSNRLYQAFAPDGGRRFSRGFGAESGRRIAPRGPGSGQQGRLSTGGGRRGGPPSTGPAMARGPYMAA
SPQLAMSPIMRGQRSAISRREALILQCLINHPWLLHDHLEEIAALELAHPEAHKLRAAIIAAFAHDHHHSPDPNEQAEKM
RADLEKGGFSELLQRVERAITTPAVWAAKPGAARDDVLATWHQLVALHRQWHSLLRELKDAELALGEDPSEPNMGWLRDV
KARMAEMDGTEALIEGFGELSGRTNRNV

Sequences:

>Translated_668_residues
MARFTPEFLDELRARLPVSDVVGKRVKLKKAGREWKGLSPFQQEKTPSFYVNDQKGFYHDFSSGKHGNIFDFIMETEGCS
FPEAVERLAGLAGLALPVATPDAAREARQRKTLYDVMELAAKFFADTLASRHGARARGYLADRAILPQTQLQFRLGYAPP
DRFALKEHLGAQGVPVNDMVEAGLLIGGEDIPVPYDRFKDRVMFPITDLRGRVIAFGGRAMQKDVPAKYLNSPETPLFHK
GDNLYNLASARQAAHNGAPMIVVEGYVDVIALVAAGFAGAVAPLGTALTESQLSLIWKMVDEPILCFDGDRAGQKAAHRA
ADIALPLLKPGKSLRFALLPEGQDPDDLVRSGGRGAVEEVLKIAKPLAEVIWSREIEGGSFNTPERRAALEARIGQLTGA
IADEVVRRYYKQDLSNRLYQAFAPDGGRRFSRGFGAESGRRIAPRGPGSGQQGRLSTGGGRRGGPPSTGPAMARGPYMAA
SPQLAMSPIMRGQRSAISRREALILQCLINHPWLLHDHLEEIAALELAHPEAHKLRAAIIAAFAHDHHHSPDPNEQAEKM
RADLEKGGFSELLQRVERAITTPAVWAAKPGAARDDVLATWHQLVALHRQWHSLLRELKDAELALGEDPSEPNMGWLRDV
KARMAEMDGTEALIEGFGELSGRTNRNV
>Mature_667_residues
ARFTPEFLDELRARLPVSDVVGKRVKLKKAGREWKGLSPFQQEKTPSFYVNDQKGFYHDFSSGKHGNIFDFIMETEGCSF
PEAVERLAGLAGLALPVATPDAAREARQRKTLYDVMELAAKFFADTLASRHGARARGYLADRAILPQTQLQFRLGYAPPD
RFALKEHLGAQGVPVNDMVEAGLLIGGEDIPVPYDRFKDRVMFPITDLRGRVIAFGGRAMQKDVPAKYLNSPETPLFHKG
DNLYNLASARQAAHNGAPMIVVEGYVDVIALVAAGFAGAVAPLGTALTESQLSLIWKMVDEPILCFDGDRAGQKAAHRAA
DIALPLLKPGKSLRFALLPEGQDPDDLVRSGGRGAVEEVLKIAKPLAEVIWSREIEGGSFNTPERRAALEARIGQLTGAI
ADEVVRRYYKQDLSNRLYQAFAPDGGRRFSRGFGAESGRRIAPRGPGSGQQGRLSTGGGRRGGPPSTGPAMARGPYMAAS
PQLAMSPIMRGQRSAISRREALILQCLINHPWLLHDHLEEIAALELAHPEAHKLRAAIIAAFAHDHHHSPDPNEQAEKMR
ADLEKGGFSELLQRVERAITTPAVWAAKPGAARDDVLATWHQLVALHRQWHSLLRELKDAELALGEDPSEPNMGWLRDVK
ARMAEMDGTEALIEGFGELSGRTNRNV

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=413, Percent_Identity=41.1622276029056, Blast_Score=317, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 73143; Mature: 73012

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFTPEFLDELRARLPVSDVVGKRVKLKKAGREWKGLSPFQQEKTPSFYVNDQKGFYHD
CCCCCHHHHHHHHHHCCHHHHHCCHHHHHHCCCCCCCCCCHHHHCCCCEEECCCCCCCCC
FSSGKHGNIFDFIMETEGCSFPEAVERLAGLAGLALPVATPDAAREARQRKTLYDVMELA
CCCCCCCCHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AKFFADTLASRHGARARGYLADRAILPQTQLQFRLGYAPPDRFALKEHLGAQGVPVNDMV
HHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHEEECCCCCHHHHHHHHCCCCCCCHHHHH
EAGLLIGGEDIPVPYDRFKDRVMFPITDLRGRVIAFGGRAMQKDVPAKYLNSPETPLFHK
HCCEEECCCCCCCCHHHHCCCEEEEECCCCCCEEEECCCHHHHCCCHHHCCCCCCCCEEC
GDNLYNLASARQAAHNGAPMIVVEGYVDVIALVAAGFAGAVAPLGTALTESQLSLIWKMV
CCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEPILCFDGDRAGQKAAHRAADIALPLLKPGKSLRFALLPEGQDPDDLVRSGGRGAVEEV
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHCCCCHHHHHH
LKIAKPLAEVIWSREIEGGSFNTPERRAALEARIGQLTGAIADEVVRRYYKQDLSNRLYQ
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFAPDGGRRFSRGFGAESGRRIAPRGPGSGQQGRLSTGGGRRGGPPSTGPAMARGPYMAA
HHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SPQLAMSPIMRGQRSAISRREALILQCLINHPWLLHDHLEEIAALELAHPEAHKLRAAII
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
AAFAHDHHHSPDPNEQAEKMRADLEKGGFSELLQRVERAITTPAVWAAKPGAARDDVLAT
HHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHH
WHQLVALHRQWHSLLRELKDAELALGEDPSEPNMGWLRDVKARMAEMDGTEALIEGFGEL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
SGRTNRNV
CCCCCCCC
>Mature Secondary Structure 
ARFTPEFLDELRARLPVSDVVGKRVKLKKAGREWKGLSPFQQEKTPSFYVNDQKGFYHD
CCCCHHHHHHHHHHCCHHHHHCCHHHHHHCCCCCCCCCCHHHHCCCCEEECCCCCCCCC
FSSGKHGNIFDFIMETEGCSFPEAVERLAGLAGLALPVATPDAAREARQRKTLYDVMELA
CCCCCCCCHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AKFFADTLASRHGARARGYLADRAILPQTQLQFRLGYAPPDRFALKEHLGAQGVPVNDMV
HHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHEEECCCCCHHHHHHHHCCCCCCCHHHHH
EAGLLIGGEDIPVPYDRFKDRVMFPITDLRGRVIAFGGRAMQKDVPAKYLNSPETPLFHK
HCCEEECCCCCCCCHHHHCCCEEEEECCCCCCEEEECCCHHHHCCCHHHCCCCCCCCEEC
GDNLYNLASARQAAHNGAPMIVVEGYVDVIALVAAGFAGAVAPLGTALTESQLSLIWKMV
CCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEPILCFDGDRAGQKAAHRAADIALPLLKPGKSLRFALLPEGQDPDDLVRSGGRGAVEEV
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHCCCCHHHHHH
LKIAKPLAEVIWSREIEGGSFNTPERRAALEARIGQLTGAIADEVVRRYYKQDLSNRLYQ
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFAPDGGRRFSRGFGAESGRRIAPRGPGSGQQGRLSTGGGRRGGPPSTGPAMARGPYMAA
HHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SPQLAMSPIMRGQRSAISRREALILQCLINHPWLLHDHLEEIAALELAHPEAHKLRAAII
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
AAFAHDHHHSPDPNEQAEKMRADLEKGGFSELLQRVERAITTPAVWAAKPGAARDDVLAT
HHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHH
WHQLVALHRQWHSLLRELKDAELALGEDPSEPNMGWLRDVKARMAEMDGTEALIEGFGEL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
SGRTNRNV
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]