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The map label for this gene is yafC [H]

Identifier: 146338405

GI number: 146338405

Start: 1409026

End: 1409949

Strand: Reverse

Name: yafC [H]

Synonym: BRADO1320

Alternate gene names: 146338405

Gene position: 1409949-1409026 (Counterclockwise)

Preceding gene: 146338406

Following gene: 146338404

Centisome position: 18.91

GC content: 64.5

Gene sequence:

>924_bases
ATGCCACGATCACGGGATGGATTTACGGATATGGATTGGGACAAGCTGAAGGTGTTTCACGCAGCGGCGGAAGCGGGCAG
CTTCACGCATGCCGGGGAGCAGCTCGGGCTGTCGCAATCGGCGGTCTCGCGCCAAGTCAGCGCGCTCGAGCAGGAGCTCG
CGGTCTCGCTGTTCCATCGCCACGCGCGCGGGCTGATCCTCACCGAGCAGGGTGATCTGCTGTTCCGCACGGCGCACGAC
GTCTTCATGCAGCTGCAGGCCGCGCGCGCCAAGCTGACCGACAGCCGCGAGCGGCCGAGCGGCGATCTCAAGATCACGAC
CACGCCCGGCGTCGGCATCAACTGGCTGATCCCGCGGCTCGGCGAGTTCACCGCGATGTATCCGGAGATCCGGATCTCGC
TGATCGTCACCGACGAGGAGCTCGATTTGTCGATGCGCGAGGCCGACGTTGCGATCCGCACCCGCAAGCCGACGCAGCCC
GACCTGATTCAGCGCAAGCTGTTCGCGATCAGCTTCCACGCCTACTGCTCGCCGGAATATGTGAAGCGCTTCGGCACGCC
GCGCACGCTCGACGAGCTCGACGACCATCGCATCATCATGCTGGCCGACGGCCAGGTCGCGCCGCATCTGCAGAACCGCA
ACTGGCTGGTCGATGCCGGCCGCAACGGCTCCGGTCCGCGCGAGGCCTATTTCAAGGTCAACAACATCCTCGGTCTGGTG
CGCGCCTGTCAGCAGGGCCTCGGCATTGCCGCGCTGCCGGACTATCTCGTCGAAGAAAACAGCCGTCTCGTGCAGCTGTT
CGGCGAGTCGGATTCGATTCAGCTCGACACCTACTTTGTCTATCCCGAGGAATTGAAGACGGTCGCGCGCGTGCAGGTGT
TCCGTGACTTTGTCGTGAGCAAAGCGCAGCGCTGGCCGGCCTGA

Upstream 100 bases:

>100_bases
GCGATCACCGCCGCCGGCATGGGCTGCATGGCCGCGCTAGAGGCCGAACGCTTCATCGCCCACGCGGCGAGCGAACGCGC
CGCTGCGGAATGAACTGACG

Downstream 100 bases:

>100_bases
TCCGCACATCTGACAGTGCATGTCTGACATGCGGCGCACCTGCTTGCTGCGGGGCCGTGTCGAGGACCATAGTGTCCGTG
TGCTGAGCGTTGGCGCCGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPRSRDGFTDMDWDKLKVFHAAAEAGSFTHAGEQLGLSQSAVSRQVSALEQELAVSLFHRHARGLILTEQGDLLFRTAHD
VFMQLQAARAKLTDSRERPSGDLKITTTPGVGINWLIPRLGEFTAMYPEIRISLIVTDEELDLSMREADVAIRTRKPTQP
DLIQRKLFAISFHAYCSPEYVKRFGTPRTLDELDDHRIIMLADGQVAPHLQNRNWLVDAGRNGSGPREAYFKVNNILGLV
RACQQGLGIAALPDYLVEENSRLVQLFGESDSIQLDTYFVYPEELKTVARVQVFRDFVVSKAQRWPA

Sequences:

>Translated_307_residues
MPRSRDGFTDMDWDKLKVFHAAAEAGSFTHAGEQLGLSQSAVSRQVSALEQELAVSLFHRHARGLILTEQGDLLFRTAHD
VFMQLQAARAKLTDSRERPSGDLKITTTPGVGINWLIPRLGEFTAMYPEIRISLIVTDEELDLSMREADVAIRTRKPTQP
DLIQRKLFAISFHAYCSPEYVKRFGTPRTLDELDDHRIIMLADGQVAPHLQNRNWLVDAGRNGSGPREAYFKVNNILGLV
RACQQGLGIAALPDYLVEENSRLVQLFGESDSIQLDTYFVYPEELKTVARVQVFRDFVVSKAQRWPA
>Mature_306_residues
PRSRDGFTDMDWDKLKVFHAAAEAGSFTHAGEQLGLSQSAVSRQVSALEQELAVSLFHRHARGLILTEQGDLLFRTAHDV
FMQLQAARAKLTDSRERPSGDLKITTTPGVGINWLIPRLGEFTAMYPEIRISLIVTDEELDLSMREADVAIRTRKPTQPD
LIQRKLFAISFHAYCSPEYVKRFGTPRTLDELDDHRIIMLADGQVAPHLQNRNWLVDAGRNGSGPREAYFKVNNILGLVR
ACQQGLGIAALPDYLVEENSRLVQLFGESDSIQLDTYFVYPEELKTVARVQVFRDFVVSKAQRWPA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=300, Percent_Identity=32, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=29.0969899665552, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=29.1525423728814, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=29.1525423728814, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787128, Length=192, Percent_Identity=31.25, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1789440, Length=294, Percent_Identity=25.1700680272109, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=28.4722222222222, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=26.7123287671233, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789639, Length=289, Percent_Identity=28.3737024221453, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788706, Length=223, Percent_Identity=29.1479820627803, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI157672245, Length=229, Percent_Identity=28.3842794759825, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790262, Length=253, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=23.6947791164659, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788626, Length=260, Percent_Identity=25.3846153846154, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787601, Length=195, Percent_Identity=23.5897435897436, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34639; Mature: 34508

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRSRDGFTDMDWDKLKVFHAAAEAGSFTHAGEQLGLSQSAVSRQVSALEQELAVSLFHR
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
HARGLILTEQGDLLFRTAHDVFMQLQAARAKLTDSRERPSGDLKITTTPGVGINWLIPRL
HHCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHH
GEFTAMYPEIRISLIVTDEELDLSMREADVAIRTRKPTQPDLIQRKLFAISFHAYCSPEY
HHHHEECCEEEEEEEEECCCCCCHHHHCCEEEEECCCCCHHHHHHHHHHEEEECCCCHHH
VKRFGTPRTLDELDDHRIIMLADGQVAPHLQNRNWLVDAGRNGSGPREAYFKVNNILGLV
HHHCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
RACQQGLGIAALPDYLVEENSRLVQLFGESDSIQLDTYFVYPEELKTVARVQVFRDFVVS
HHHHHCCCEECCCHHHHCCCCEEEEEECCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHH
KAQRWPA
HHHCCCC
>Mature Secondary Structure 
PRSRDGFTDMDWDKLKVFHAAAEAGSFTHAGEQLGLSQSAVSRQVSALEQELAVSLFHR
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
HARGLILTEQGDLLFRTAHDVFMQLQAARAKLTDSRERPSGDLKITTTPGVGINWLIPRL
HHCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHH
GEFTAMYPEIRISLIVTDEELDLSMREADVAIRTRKPTQPDLIQRKLFAISFHAYCSPEY
HHHHEECCEEEEEEEEECCCCCCHHHHCCEEEEECCCCCHHHHHHHHHHEEEECCCCHHH
VKRFGTPRTLDELDDHRIIMLADGQVAPHLQNRNWLVDAGRNGSGPREAYFKVNNILGLV
HHHCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
RACQQGLGIAALPDYLVEENSRLVQLFGESDSIQLDTYFVYPEELKTVARVQVFRDFVVS
HHHHHCCCEECCCHHHHCCCCEEEEEECCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHH
KAQRWPA
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]