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The map label for this gene is hemN [H]

Identifier: 146338324

GI number: 146338324

Start: 1306803

End: 1308227

Strand: Direct

Name: hemN [H]

Synonym: BRADO1229

Alternate gene names: 146338324

Gene position: 1306803-1308227 (Clockwise)

Preceding gene: 146338319

Following gene: 146338325

Centisome position: 17.53

GC content: 64.35

Gene sequence:

>1425_bases
TTGCTTGGTGTCAGACAGGTCCAAACTCCGAATGACCCCCCAAGTCGACAGGCACGAGTGGACAGGCACGACATGAGACC
CGATCTCGCGGCATGCTACGGACAAGAGCGCCTTCCGCGCTACACCAGCTATCCCACGGCACCCCATTTCTCGGCTGCGG
TCAGTCCGTCCACCTATGCCGAATGGTTGCGCGCCATTCCCGCAACCGCGACGGCATCTCTTTACCTGCACGTGCCGTTC
TGTCGGTCGATGTGCTGGTATTGCGGCTGCAACACCATGGTCGCCAAGCGCGACGAGCCGATCGCGATCTATGAGTCGGC
ATTGCGCTGCGAGATCGACATGGTCTCCCGCCAGATCGGCCGGCCGCTTCCGGTCGGGCACATCCATTTCGGCGGCGGCA
CGCCGACCATCATGGCGCCAGAGTCATTCGTCGATCTGGTGGGGTCGTTGCGCCACTCCTTCCGCGTGCTGCCGTCGGCC
GAGATCGCGATCGAGATTGACCCGCGCACGCTGACCAAGGCGATGATCGATGCGCTGGCGGGGAGTGGCGTCAATCGCGC
GAGCCTGGGTGTGCAGAGCTTCGATCCGCTCGTGCAGCATGCGATCAACCGCGTGCAGAGCTTCGAGCAGACCGCTGCGG
CCACCAATGGGCTGCGCCGGGCGGGCGTCGCGGGGATCAATTTCGACCTGATCTACGGCCTGCCGCATCAAACCGTGGCG
TCTTGTCTCGACACCGTGCGGCGCGCCATCGACCTGCGGCCAGACCGGCTCTCCGTGTTCGGCTATGCCCATGTGCCATC
CTTCAAGACGCATCAGCGCAAGATCCGGGATGACGCGCTGCCGGACAGCCTCGAACGCTACGATCAGGCCTGCGGCATCG
CGGATGCGCTGAAGGACGCGGGCTATGTTCAGATCGGCCTCGATCACTTCGCCCTGCCGACCGACCCGATGACCAACGCC
TTCCGCGAGGGACGTCTGCATCGCAATTTTCAGGGCTATACGACGGACGCCAATGACCTGCTGCTCGGCTTCGGTGCCTC
GGCGATCGGCCAGCTGCCGCAAGGCTACGTTCAGAACGCGCCTCAGGTGCGCGCCTATGAGGAGAAGATCGCCGCAGGTT
TCCTCGCCACCGTCAAGGGCTACGGCCTGACCGACGACGATCGCCTGCGCGCCGACATCATCGAGCGCATCATGTGCGAC
TATGGCGTCGACCTCGACGTCATCTGCACGCGCCACGGCACGGAAACCGACGAGATGCTGCGATCGGCTCCCCGATTGCA
GTCGTTGATTTCGGATGGCGTGGTCGAACTCAACGGCAGCGCGCTCGCCGTGACCGAGCATTCCCGCTTCCTGGTGCGCA
GCGTAGCCGCCGCGTTCGACGCGCATCTGGACGCATCGAAGCAGCTGCACAGCCGGGCTGTGTAG

Upstream 100 bases:

>100_bases
CACTTTGACGAAGCGCAATGTCGCTGGCGAAAGCCGGGTTTTTACTGGCAGGGACAGGGCATTCGTCGGCCAGCCTTGAG
GAAGCTCAAGGCAGCGTCGG

Downstream 100 bases:

>100_bases
CGCGCGCCACGCCGCGGTGCTTGTGCCGCGACAAAGAGACGGGACGGCATCGCTGCTACCATTCGCTTTCGAATGGAGGC
CCGCATGCCCATCTCCTTTG

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MLGVRQVQTPNDPPSRQARVDRHDMRPDLAACYGQERLPRYTSYPTAPHFSAAVSPSTYAEWLRAIPATATASLYLHVPF
CRSMCWYCGCNTMVAKRDEPIAIYESALRCEIDMVSRQIGRPLPVGHIHFGGGTPTIMAPESFVDLVGSLRHSFRVLPSA
EIAIEIDPRTLTKAMIDALAGSGVNRASLGVQSFDPLVQHAINRVQSFEQTAAATNGLRRAGVAGINFDLIYGLPHQTVA
SCLDTVRRAIDLRPDRLSVFGYAHVPSFKTHQRKIRDDALPDSLERYDQACGIADALKDAGYVQIGLDHFALPTDPMTNA
FREGRLHRNFQGYTTDANDLLLGFGASAIGQLPQGYVQNAPQVRAYEEKIAAGFLATVKGYGLTDDDRLRADIIERIMCD
YGVDLDVICTRHGTETDEMLRSAPRLQSLISDGVVELNGSALAVTEHSRFLVRSVAAAFDAHLDASKQLHSRAV

Sequences:

>Translated_474_residues
MLGVRQVQTPNDPPSRQARVDRHDMRPDLAACYGQERLPRYTSYPTAPHFSAAVSPSTYAEWLRAIPATATASLYLHVPF
CRSMCWYCGCNTMVAKRDEPIAIYESALRCEIDMVSRQIGRPLPVGHIHFGGGTPTIMAPESFVDLVGSLRHSFRVLPSA
EIAIEIDPRTLTKAMIDALAGSGVNRASLGVQSFDPLVQHAINRVQSFEQTAAATNGLRRAGVAGINFDLIYGLPHQTVA
SCLDTVRRAIDLRPDRLSVFGYAHVPSFKTHQRKIRDDALPDSLERYDQACGIADALKDAGYVQIGLDHFALPTDPMTNA
FREGRLHRNFQGYTTDANDLLLGFGASAIGQLPQGYVQNAPQVRAYEEKIAAGFLATVKGYGLTDDDRLRADIIERIMCD
YGVDLDVICTRHGTETDEMLRSAPRLQSLISDGVVELNGSALAVTEHSRFLVRSVAAAFDAHLDASKQLHSRAV
>Mature_474_residues
MLGVRQVQTPNDPPSRQARVDRHDMRPDLAACYGQERLPRYTSYPTAPHFSAAVSPSTYAEWLRAIPATATASLYLHVPF
CRSMCWYCGCNTMVAKRDEPIAIYESALRCEIDMVSRQIGRPLPVGHIHFGGGTPTIMAPESFVDLVGSLRHSFRVLPSA
EIAIEIDPRTLTKAMIDALAGSGVNRASLGVQSFDPLVQHAINRVQSFEQTAAATNGLRRAGVAGINFDLIYGLPHQTVA
SCLDTVRRAIDLRPDRLSVFGYAHVPSFKTHQRKIRDDALPDSLERYDQACGIADALKDAGYVQIGLDHFALPTDPMTNA
FREGRLHRNFQGYTTDANDLLLGFGASAIGQLPQGYVQNAPQVRAYEEKIAAGFLATVKGYGLTDDDRLRADIIERIMCD
YGVDLDVICTRHGTETDEMLRSAPRLQSLISDGVVELNGSALAVTEHSRFLVRSVAAAFDAHLDASKQLHSRAV

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=294, Percent_Identity=26.530612244898, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI87082341, Length=443, Percent_Identity=39.0519187358916, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI1789325, Length=403, Percent_Identity=26.5508684863524, Blast_Score=105, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004558
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 52007; Mature: 52007

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGVRQVQTPNDPPSRQARVDRHDMRPDLAACYGQERLPRYTSYPTAPHFSAAVSPSTYA
CCCCCCCCCCCCCCCHHHHCCHHHCCCHHHHHHCHHHCCCCCCCCCCCCCCCCCCHHHHH
EWLRAIPATATASLYLHVPFCRSMCWYCGCNTMVAKRDEPIAIYESALRCEIDMVSRQIG
HHHHHCCCCCEEEEEEECHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHC
RPLPVGHIHFGGGTPTIMAPESFVDLVGSLRHSFRVLPSAEIAIEIDPRTLTKAMIDALA
CCCCCCEEEECCCCCCEECCHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHH
GSGVNRASLGVQSFDPLVQHAINRVQSFEQTAAATNGLRRAGVAGINFDLIYGLPHQTVA
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHH
SCLDTVRRAIDLRPDRLSVFGYAHVPSFKTHQRKIRDDALPDSLERYDQACGIADALKDA
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCC
GYVQIGLDHFALPTDPMTNAFREGRLHRNFQGYTTDANDLLLGFGASAIGQLPQGYVQNA
CEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHEEECCHHHHHHCCHHHHCCC
PQVRAYEEKIAAGFLATVKGYGLTDDDRLRADIIERIMCDYGVDLDVICTRHGTETDEML
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
RSAPRLQSLISDGVVELNGSALAVTEHSRFLVRSVAAAFDAHLDASKQLHSRAV
HHHHHHHHHHHCCCEEECCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MLGVRQVQTPNDPPSRQARVDRHDMRPDLAACYGQERLPRYTSYPTAPHFSAAVSPSTYA
CCCCCCCCCCCCCCCHHHHCCHHHCCCHHHHHHCHHHCCCCCCCCCCCCCCCCCCHHHHH
EWLRAIPATATASLYLHVPFCRSMCWYCGCNTMVAKRDEPIAIYESALRCEIDMVSRQIG
HHHHHCCCCCEEEEEEECHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHC
RPLPVGHIHFGGGTPTIMAPESFVDLVGSLRHSFRVLPSAEIAIEIDPRTLTKAMIDALA
CCCCCCEEEECCCCCCEECCHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHH
GSGVNRASLGVQSFDPLVQHAINRVQSFEQTAAATNGLRRAGVAGINFDLIYGLPHQTVA
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHH
SCLDTVRRAIDLRPDRLSVFGYAHVPSFKTHQRKIRDDALPDSLERYDQACGIADALKDA
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCC
GYVQIGLDHFALPTDPMTNAFREGRLHRNFQGYTTDANDLLLGFGASAIGQLPQGYVQNA
CEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHEEECCHHHHHHCCHHHHCCC
PQVRAYEEKIAAGFLATVKGYGLTDDDRLRADIIERIMCDYGVDLDVICTRHGTETDEML
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
RSAPRLQSLISDGVVELNGSALAVTEHSRFLVRSVAAAFDAHLDASKQLHSRAV
HHHHHHHHHHHCCCEEECCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11157943; 12597275 [H]