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The map label for this gene is pyk [H]

Identifier: 146338281

GI number: 146338281

Start: 1260092

End: 1261528

Strand: Reverse

Name: pyk [H]

Synonym: BRADO1185

Alternate gene names: 146338281

Gene position: 1261528-1260092 (Counterclockwise)

Preceding gene: 146338282

Following gene: 146338273

Centisome position: 16.92

GC content: 68.75

Gene sequence:

>1437_bases
ATGAGACGCCTGCGCCGCATCAAGATTCTCGCCACGCTCGGTCCCGCCTCCTCGGACAGCGCGATGATTCGCAAGCTGTT
CGAGGCCGGGGCCGACATCTTCCGCATCAACATGAGCCACACCCCGCATGACAAGCTGCGGGAGCTGGTGGCCACGATCC
GCAGCGTCGAAGCCAGCTATGGCCGGCCGATCGGCATCCTGGTCGATCTGCAGGGCCCCAAACTGCGGCTTGGCACGTTT
GCCGAAGGTCCGGTCCAGCTCAACAACGGACAGAGCTTCGTGCTCGATTCCGACAAGACGCCCGGCGATGCGACACGGGT
GTACCTGCCGCATCCGGAGATCCTGGCAGCCCTGCAGCCCGGCCACGCGCTGCTGCTCGACGACGGCAAGGTGCGGCTGA
TCGCCGAGGAGACGACCCCGACGCGCGCCGTCACCCGCGTCGTCATCGGCGGCAAGATGTCCGACCGCAAGGGCGTGAGC
CTGCCCGACACCGATCTGCCGGTCTCGGCGATGACGCCGAAGGACCGCGCCGATCTCGAAGCGGCGCTGAACATCGGCAT
CGACTGGATCGCGCTGTCCTTCGTGCAGCGCGCCGATGACGTGCTCGAGGCGAAGAAGATCATCCGCGGCCGCGCCGCCG
TGATGTCCAAGATCGAGAAACCGCAGGCGATCGAGCGTCTCAACGACATCGTCGATGTCTCCGACGCGCTGATGGTGGCG
CGCGGCGATCTCGGCGTCGAGCTGCCGCTGGAGCGCGTGCCGGGCCTGCAGAAGCAGATGACACGGTTGGCGCGCCGCGC
CGGCAAGCCGGTGGTGGTGGCGACGCAGATGCTGGAATCGATGATCCAGTCGCCGGTCCCGACCCGCGCGGAGGTCTCCG
ACGTGGCGACCGCGGTCTATGAGGGCGCTGACGCGATCATGCTGTCGGCGGAATCGGCGGCCGGCAAGTTTCCGGTCGAG
GCGGTCTCGACGATGAACCGGATCGGCGAGGAGGTCGAACGCGACATCGTCTATCGCTCGGTGATCGCGGCGCAGCGGCC
CGAGCCGGAGTCGACCGCGGGCGACGCCATCGCCGAGGCGGCGCGCAGGATTGCCGAAAATCTCGATCTGCCGGCGATCA
TCTGCTGGACGTCGTCCGGCTCGACCGCGGTCCGCGTCGCGCGCGAGCGGCCCAAGCCGCCGGTGGTCGCGATCACCCCG
AACCTGGCGACCGGGCGCCGCCTCGCGGTGGTGTGGGGCGTCCATTGCGTGGTGGCCGAGGATGCCCGCGACCAGGACGA
CATGGTCGAACGTGCCGGCTCGATCGCGTTTCGCGACGGCTTCGTCCGCGCCGGCCAGCGCGTCATCGTCGTTGCCGGCG
TCCCGCTCGGCACCCCCGGCACCACCAACATGGTGCGCATCGCCTATGTCGGGCCGTCGAACGAGGCCCATCTCTGA

Upstream 100 bases:

>100_bases
GTCCGCTCGGCCCCGGCAATGTCCCTGGCCTGCCCAATGGTCCTCAGGCGGCGCCGCCTGCCGGATCCGGCCTGCCGTCC
GGGCCGGCAGCGCCCAAACC

Downstream 100 bases:

>100_bases
GCTGATCAGTCGGCGCTGCCGACCGGCTTGAGACCCGCGGTCGAAGCCGCGGGCAGCGCGGCCGAGATCACCGGACAGAC
CATGATGTCGGTGCCATCGG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MRRLRRIKILATLGPASSDSAMIRKLFEAGADIFRINMSHTPHDKLRELVATIRSVEASYGRPIGILVDLQGPKLRLGTF
AEGPVQLNNGQSFVLDSDKTPGDATRVYLPHPEILAALQPGHALLLDDGKVRLIAEETTPTRAVTRVVIGGKMSDRKGVS
LPDTDLPVSAMTPKDRADLEAALNIGIDWIALSFVQRADDVLEAKKIIRGRAAVMSKIEKPQAIERLNDIVDVSDALMVA
RGDLGVELPLERVPGLQKQMTRLARRAGKPVVVATQMLESMIQSPVPTRAEVSDVATAVYEGADAIMLSAESAAGKFPVE
AVSTMNRIGEEVERDIVYRSVIAAQRPEPESTAGDAIAEAARRIAENLDLPAIICWTSSGSTAVRVARERPKPPVVAITP
NLATGRRLAVVWGVHCVVAEDARDQDDMVERAGSIAFRDGFVRAGQRVIVVAGVPLGTPGTTNMVRIAYVGPSNEAHL

Sequences:

>Translated_478_residues
MRRLRRIKILATLGPASSDSAMIRKLFEAGADIFRINMSHTPHDKLRELVATIRSVEASYGRPIGILVDLQGPKLRLGTF
AEGPVQLNNGQSFVLDSDKTPGDATRVYLPHPEILAALQPGHALLLDDGKVRLIAEETTPTRAVTRVVIGGKMSDRKGVS
LPDTDLPVSAMTPKDRADLEAALNIGIDWIALSFVQRADDVLEAKKIIRGRAAVMSKIEKPQAIERLNDIVDVSDALMVA
RGDLGVELPLERVPGLQKQMTRLARRAGKPVVVATQMLESMIQSPVPTRAEVSDVATAVYEGADAIMLSAESAAGKFPVE
AVSTMNRIGEEVERDIVYRSVIAAQRPEPESTAGDAIAEAARRIAENLDLPAIICWTSSGSTAVRVARERPKPPVVAITP
NLATGRRLAVVWGVHCVVAEDARDQDDMVERAGSIAFRDGFVRAGQRVIVVAGVPLGTPGTTNMVRIAYVGPSNEAHL
>Mature_478_residues
MRRLRRIKILATLGPASSDSAMIRKLFEAGADIFRINMSHTPHDKLRELVATIRSVEASYGRPIGILVDLQGPKLRLGTF
AEGPVQLNNGQSFVLDSDKTPGDATRVYLPHPEILAALQPGHALLLDDGKVRLIAEETTPTRAVTRVVIGGKMSDRKGVS
LPDTDLPVSAMTPKDRADLEAALNIGIDWIALSFVQRADDVLEAKKIIRGRAAVMSKIEKPQAIERLNDIVDVSDALMVA
RGDLGVELPLERVPGLQKQMTRLARRAGKPVVVATQMLESMIQSPVPTRAEVSDVATAVYEGADAIMLSAESAAGKFPVE
AVSTMNRIGEEVERDIVYRSVIAAQRPEPESTAGDAIAEAARRIAENLDLPAIICWTSSGSTAVRVARERPKPPVVAITP
NLATGRRLAVVWGVHCVVAEDARDQDDMVERAGSIAFRDGFVRAGQRVIVVAGVPLGTPGTTNMVRIAYVGPSNEAHL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=491, Percent_Identity=36.6598778004073, Blast_Score=278, Evalue=9e-75,
Organism=Homo sapiens, GI10835121, Length=491, Percent_Identity=36.6598778004073, Blast_Score=278, Evalue=9e-75,
Organism=Homo sapiens, GI33286422, Length=486, Percent_Identity=34.7736625514403, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI33286420, Length=486, Percent_Identity=34.7736625514403, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI33286418, Length=491, Percent_Identity=35.234215885947, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=31.3588850174216, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=31.3588850174216, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=27.0042194092827, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=27.0042194092827, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=26.1682242990654, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1788160, Length=483, Percent_Identity=39.5445134575569, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI1787965, Length=466, Percent_Identity=33.9055793991416, Blast_Score=279, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=34.504132231405, Blast_Score=275, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI17506829, Length=483, Percent_Identity=35.1966873706004, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI71984413, Length=483, Percent_Identity=35.1966873706004, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI71984406, Length=483, Percent_Identity=35.1966873706004, Blast_Score=259, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17506831, Length=483, Percent_Identity=35.1966873706004, Blast_Score=258, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=32.5773195876289, Blast_Score=240, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6324923, Length=492, Percent_Identity=32.1138211382114, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=34.6938775510204, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=34.6938775510204, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24648966, Length=425, Percent_Identity=33.4117647058824, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24581235, Length=485, Percent_Identity=30.3092783505155, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24646914, Length=240, Percent_Identity=36.25, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51452; Mature: 51452

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLRRIKILATLGPASSDSAMIRKLFEAGADIFRINMSHTPHDKLRELVATIRSVEASY
CCCHHEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHC
GRPIGILVDLQGPKLRLGTFAEGPVQLNNGQSFVLDSDKTPGDATRVYLPHPEILAALQP
CCCEEEEEECCCCCEEECCCCCCCEEECCCCEEEEECCCCCCCCEEEEECCHHHHHEECC
GHALLLDDGKVRLIAEETTPTRAVTRVVIGGKMSDRKGVSLPDTDLPVSAMTPKDRADLE
CCEEEEECCCEEEEEECCCCHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
AALNIGIDWIALSFVQRADDVLEAKKIIRGRAAVMSKIEKPQAIERLNDIVDVSDALMVA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEE
RGDLGVELPLERVPGLQKQMTRLARRAGKPVVVATQMLESMIQSPVPTRAEVSDVATAVY
ECCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EGADAIMLSAESAAGKFPVEAVSTMNRIGEEVERDIVYRSVIAAQRPEPESTAGDAIAEA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ARRIAENLDLPAIICWTSSGSTAVRVARERPKPPVVAITPNLATGRRLAVVWGVHCVVAE
HHHHHHCCCCCEEEEEECCCCCEEEEEHHCCCCCEEEECCCCCCCCEEEEEEEEEEEEEC
DARDQDDMVERAGSIAFRDGFVRAGQRVIVVAGVPLGTPGTTNMVRIAYVGPSNEAHL
CCCCHHHHHHHHCCEEEECHHHHCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MRRLRRIKILATLGPASSDSAMIRKLFEAGADIFRINMSHTPHDKLRELVATIRSVEASY
CCCHHEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHC
GRPIGILVDLQGPKLRLGTFAEGPVQLNNGQSFVLDSDKTPGDATRVYLPHPEILAALQP
CCCEEEEEECCCCCEEECCCCCCCEEECCCCEEEEECCCCCCCCEEEEECCHHHHHEECC
GHALLLDDGKVRLIAEETTPTRAVTRVVIGGKMSDRKGVSLPDTDLPVSAMTPKDRADLE
CCEEEEECCCEEEEEECCCCHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
AALNIGIDWIALSFVQRADDVLEAKKIIRGRAAVMSKIEKPQAIERLNDIVDVSDALMVA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEE
RGDLGVELPLERVPGLQKQMTRLARRAGKPVVVATQMLESMIQSPVPTRAEVSDVATAVY
ECCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EGADAIMLSAESAAGKFPVEAVSTMNRIGEEVERDIVYRSVIAAQRPEPESTAGDAIAEA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ARRIAENLDLPAIICWTSSGSTAVRVARERPKPPVVAITPNLATGRRLAVVWGVHCVVAE
HHHHHHCCCCCEEEEEECCCCCEEEEEHHCCCCCEEEECCCCCCCCEEEEEEEEEEEEEC
DARDQDDMVERAGSIAFRDGFVRAGQRVIVVAGVPLGTPGTTNMVRIAYVGPSNEAHL
CCCCHHHHHHHHCCEEEECHHHHCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA