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The map label for this gene is nirA [H]

Identifier: 146338169

GI number: 146338169

Start: 1134504

End: 1136159

Strand: Direct

Name: nirA [H]

Synonym: BRADO1067

Alternate gene names: 146338169

Gene position: 1134504-1136159 (Clockwise)

Preceding gene: 146338168

Following gene: 146338170

Centisome position: 15.21

GC content: 64.01

Gene sequence:

>1656_bases
ATGTATGCTTATGACGACATCGACCGCACTTTGGTCAACGAGCGCGTCGCCGAATTCCGCGATCAGGTGAAGCGCCGTCT
CTCGGGCGAGCTCACCGAGGACGAGTTCAAGATGCTGCGGCTGCAGAACGGCGTGTATCTGCAGCTGCACGCCTACATGT
ACCGCATCGCCATTCCCTACGGCACGCTGTCGTCGGATCAGCTGCGCCGGCTGGCGCATGTCGCGCGCCGCTACGATCGC
GGCTACGGCCACTTCACGACCCGGCAAAACATCCAGTTCAACTGGATCAAGCTCGCCGAGCTGCCGGATGCGATGGCCGA
TCTGGCCGAGGTCGGCATCCAGTCGATGCAGACCTCCGGCAACAACATGCGCAACGTCACCTCCGACCAGTGGGCCGGCG
TCGCGCCGGGCGAGGTCGAGGATCCCCGCATCTGGGCCGAGCTGCTGCGCCAGTTCACGACCCTGCATCCGGAATTCTCG
TTCCTGCCGCGCAAGTTCAAGATCGCGATCACGGCGTCCGAGCACGATCGCGCCGCGATCAAGATCCACGACATCGGCCT
GCGTCTGAAGAAGAACGAGCAGGGCGAGACCGGCTTCGAGGTGCTGGTCGGCGGCGGCCTCGGCCGTTCGCCCTTCATCG
CCAAGACCATCAAGCCGTTCGTGCACGGCCACGACATTCTGAGCTATGTCGAGGCGATCCTGCGCGTCTACAATCAGTAC
GGCCGCCGTGACAACATCTACAAGGCGCGCATCAAGATCCTGGTCCACGAGCTTGGCATCGAGAAGTTCGCGGCCGAGGT
CGAGGAGGAATGGCAGCAGATCCGCGACGGCGTGCTCAAGCTCGATCAGGGCATCGTCGAGGACATCCGCTCGCGCTTCT
TCTATCCGGCCTACGAGACGCTGCCGCACATGCCGGACGAGCTGAAGCAGGCCGCCCATGATGCCAAGTTCGAGGCCTGG
CGCAAGAACTCTGTCTTTCCGCACAAGCAGCAGGGCTACGCGATCGCCGTGGTGTCGCTGAAGCCGGTCGGCAAGCCGCC
GGGCGACGCGACCGCCGACCAGATGGATGCGCTGGCCGACATTGCCGACAGATACTCGTTCGGCGAGATCCGCGTCGGCC
ACGAGCAAAATCTGGCGCTGCCGCATGTGCGCAAGCGCGACCTGCCGGCGGTGTGGAAGGCGCTCGAGGCGATCGGGCTC
GCCATCCCGAACGTCAACCTCGTCTCGGACATCATCGCCTGCCCGGGCCTGGACTACTGCTCGCTCGCCAATGCGCGCTC
GATTCCGATCGCGCAGGAGATCACGCGCCGCTTCGCCAACCACGACACCGCGAACCTGATCGGCCGGCTGCACATCAATA
TCTCCGGCTGCATCAATGCCTGCGGCCACCATCATGTCGGGCACATCGGCATCCTCGGCGTCGAGAAGAACAACGAGGAA
TTCTATCAGATCACCATCGGCGGCCGCGCCGACGAGGGCGCCGAGCTCGGCACCTTGATCGGCCCTGCGGTGCCCTACGC
CGAAGTTGCCGATGTCGTCGAGGACATCGTCGAAGCCTATCTTGCGCTGCGCGTGAGCCCTGAGGAGCTGTTCGTCGACA
CGGTGAAGCGGCTCGGGGTCGAACCATTCAGGGAGCGGGTCTATGCCACTCGTTAA

Upstream 100 bases:

>100_bases
GTCGCCGCCGACGGCGCGGTGAAGCCCGGCAACATGCGCGAACACATCCGGCTGACGGGCGCCACCATCGCGCTCCCGGC
TCAAGCCTGAAGGCCTTCTC

Downstream 100 bases:

>100_bases
GGGCGGACACATCATCGCGGACGAGTTCGTCCGCGTCACCGACGATGCCGAGCTGCCGGCGCAAGGCGCGATCCTCGTCT
CCGAGACGCGGTTTCTTGCC

Product: sulfite/ferredoxin-nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MYAYDDIDRTLVNERVAEFRDQVKRRLSGELTEDEFKMLRLQNGVYLQLHAYMYRIAIPYGTLSSDQLRRLAHVARRYDR
GYGHFTTRQNIQFNWIKLAELPDAMADLAEVGIQSMQTSGNNMRNVTSDQWAGVAPGEVEDPRIWAELLRQFTTLHPEFS
FLPRKFKIAITASEHDRAAIKIHDIGLRLKKNEQGETGFEVLVGGGLGRSPFIAKTIKPFVHGHDILSYVEAILRVYNQY
GRRDNIYKARIKILVHELGIEKFAAEVEEEWQQIRDGVLKLDQGIVEDIRSRFFYPAYETLPHMPDELKQAAHDAKFEAW
RKNSVFPHKQQGYAIAVVSLKPVGKPPGDATADQMDALADIADRYSFGEIRVGHEQNLALPHVRKRDLPAVWKALEAIGL
AIPNVNLVSDIIACPGLDYCSLANARSIPIAQEITRRFANHDTANLIGRLHINISGCINACGHHHVGHIGILGVEKNNEE
FYQITIGGRADEGAELGTLIGPAVPYAEVADVVEDIVEAYLALRVSPEELFVDTVKRLGVEPFRERVYATR

Sequences:

>Translated_551_residues
MYAYDDIDRTLVNERVAEFRDQVKRRLSGELTEDEFKMLRLQNGVYLQLHAYMYRIAIPYGTLSSDQLRRLAHVARRYDR
GYGHFTTRQNIQFNWIKLAELPDAMADLAEVGIQSMQTSGNNMRNVTSDQWAGVAPGEVEDPRIWAELLRQFTTLHPEFS
FLPRKFKIAITASEHDRAAIKIHDIGLRLKKNEQGETGFEVLVGGGLGRSPFIAKTIKPFVHGHDILSYVEAILRVYNQY
GRRDNIYKARIKILVHELGIEKFAAEVEEEWQQIRDGVLKLDQGIVEDIRSRFFYPAYETLPHMPDELKQAAHDAKFEAW
RKNSVFPHKQQGYAIAVVSLKPVGKPPGDATADQMDALADIADRYSFGEIRVGHEQNLALPHVRKRDLPAVWKALEAIGL
AIPNVNLVSDIIACPGLDYCSLANARSIPIAQEITRRFANHDTANLIGRLHINISGCINACGHHHVGHIGILGVEKNNEE
FYQITIGGRADEGAELGTLIGPAVPYAEVADVVEDIVEAYLALRVSPEELFVDTVKRLGVEPFRERVYATR
>Mature_551_residues
MYAYDDIDRTLVNERVAEFRDQVKRRLSGELTEDEFKMLRLQNGVYLQLHAYMYRIAIPYGTLSSDQLRRLAHVARRYDR
GYGHFTTRQNIQFNWIKLAELPDAMADLAEVGIQSMQTSGNNMRNVTSDQWAGVAPGEVEDPRIWAELLRQFTTLHPEFS
FLPRKFKIAITASEHDRAAIKIHDIGLRLKKNEQGETGFEVLVGGGLGRSPFIAKTIKPFVHGHDILSYVEAILRVYNQY
GRRDNIYKARIKILVHELGIEKFAAEVEEEWQQIRDGVLKLDQGIVEDIRSRFFYPAYETLPHMPDELKQAAHDAKFEAW
RKNSVFPHKQQGYAIAVVSLKPVGKPPGDATADQMDALADIADRYSFGEIRVGHEQNLALPHVRKRDLPAVWKALEAIGL
AIPNVNLVSDIIACPGLDYCSLANARSIPIAQEITRRFANHDTANLIGRLHINISGCINACGHHHVGHIGILGVEKNNEE
FYQITIGGRADEGAELGTLIGPAVPYAEVADVVEDIVEAYLALRVSPEELFVDTVKRLGVEPFRERVYATR

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=287, Percent_Identity=25.0871080139373, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 62222; Mature: 62222

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAYDDIDRTLVNERVAEFRDQVKRRLSGELTEDEFKMLRLQNGVYLQLHAYMYRIAIPY
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEEEEHHEECC
GTLSSDQLRRLAHVARRYDRGYGHFTTRQNIQFNWIKLAELPDAMADLAEVGIQSMQTSG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHCCC
NNMRNVTSDQWAGVAPGEVEDPRIWAELLRQFTTLHPEFSFLPRKFKIAITASEHDRAAI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCCEEEEEEEECCCCCEEE
KIHDIGLRLKKNEQGETGFEVLVGGGLGRSPFIAKTIKPFVHGHDILSYVEAILRVYNQY
EEEECCEEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRRDNIYKARIKILVHELGIEKFAAEVEEEWQQIRDGVLKLDQGIVEDIRSRFFYPAYET
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LPHMPDELKQAAHDAKFEAWRKNSVFPHKQQGYAIAVVSLKPVGKPPGDATADQMDALAD
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHH
IADRYSFGEIRVGHEQNLALPHVRKRDLPAVWKALEAIGLAIPNVNLVSDIIACPGLDYC
HHHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHH
SLANARSIPIAQEITRRFANHDTANLIGRLHINISGCINACGHHHVGHIGILGVEKNNEE
HCCCCCCCCHHHHHHHHHHCCCHHHEEEEEEEEHHHHHHHCCCCCCCEEEEEEEECCCCE
FYQITIGGRADEGAELGTLIGPAVPYAEVADVVEDIVEAYLALRVSPEELFVDTVKRLGV
EEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
EPFRERVYATR
CHHHHHHCCCC
>Mature Secondary Structure
MYAYDDIDRTLVNERVAEFRDQVKRRLSGELTEDEFKMLRLQNGVYLQLHAYMYRIAIPY
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEEEEHHEECC
GTLSSDQLRRLAHVARRYDRGYGHFTTRQNIQFNWIKLAELPDAMADLAEVGIQSMQTSG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHCCC
NNMRNVTSDQWAGVAPGEVEDPRIWAELLRQFTTLHPEFSFLPRKFKIAITASEHDRAAI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCCEEEEEEEECCCCCEEE
KIHDIGLRLKKNEQGETGFEVLVGGGLGRSPFIAKTIKPFVHGHDILSYVEAILRVYNQY
EEEECCEEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRRDNIYKARIKILVHELGIEKFAAEVEEEWQQIRDGVLKLDQGIVEDIRSRFFYPAYET
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LPHMPDELKQAAHDAKFEAWRKNSVFPHKQQGYAIAVVSLKPVGKPPGDATADQMDALAD
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHH
IADRYSFGEIRVGHEQNLALPHVRKRDLPAVWKALEAIGLAIPNVNLVSDIIACPGLDYC
HHHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHH
SLANARSIPIAQEITRRFANHDTANLIGRLHINISGCINACGHHHVGHIGILGVEKNNEE
HCCCCCCCCHHHHHHHHHHCCCHHHEEEEEEEEHHHHHHHCCCCCCCEEEEEEEECCCCE
FYQITIGGRADEGAELGTLIGPAVPYAEVADVVEDIVEAYLALRVSPEELFVDTVKRLGV
EEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
EPFRERVYATR
CHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7766873 [H]