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The map label for this gene is cysG [H]

Identifier: 146338167

GI number: 146338167

Start: 1132748

End: 1134178

Strand: Direct

Name: cysG [H]

Synonym: BRADO1065

Alternate gene names: 146338167

Gene position: 1132748-1134178 (Clockwise)

Preceding gene: 146338164

Following gene: 146338168

Centisome position: 15.19

GC content: 70.09

Gene sequence:

>1431_bases
ATGCAATATCTGCCGGTGTTTCTCGATCTGAAGACCGGCCCCGTGCTGCTGATCGGCGCGGGCGAGCTCGTGCGCGCGAA
GCTTAGAGTCTTGCTGTCCGCGGGTGCGCGCATCCGCTGGTTCGCGACCGATGGCGATTTCGATCTCGGAGGATTGCACG
ACGATCATGGCCAGATCACGCGCGCTGACGGCGATCCGCTGACCACCGACCTGTCGGGCGTCATCGCCGTGCTGTGCGCG
GGCGCCGGCGATATCGGCGTGGCGATGTCGGCGCGGGCGCGCAGCGTCGGCCTGCCGGTCAACGTGATGGACGACCTCGC
GCATTCCAGCTTCATCTTCCCGGCGATCGTCGATCGCGGCGACGTCGTGGTGGCGATCGGCACCGGCGGCTCGTCGCCCG
TGGTCGCGCGCCGCGTGCGCGAGCGCATCGAGGCGCTGCTGCCGGCGCGGATCGGCGAGCTCGCGGGTTTCATCGGCGAC
TTCCGCAAGATCATCAACCCGCGGATCGCGGAGATGCCGCTGCGCCGTCGCTTCTGGGAGCGTGTTGTCGATGGCCCGAT
CGGCGCGCTGGTGCTGGCCGGCCGCAAGCACGACGCCGAGGCGGCGCTGAACAGCATCGCCGATCCTACCGCATTCGCGG
GGGCCGATCATGCCGGCCGCGGCGAGGGGCATGTGACCCTGGTCGGCGCCGGGCCTGGCGATCCCGATCTCTTGACGATC
AAGGCGCTGCGCGCGCTGCAGGACGCCGACGTCGTGTTCTACGACGAGCTGGTGACGCCGGAGATTCTCGACCGCATCCG
CCGCGACGCCTCGCGCGTTCCGGTGGGCCGCCGCGTCGGCAAGCCCGGCATCGGCCAGGACGCGGTGAACAAGCTGCTGA
TCGACGCCGCGCGCGAGGGCAAGCGCGCGGTGCGCCTGAAGGGCGGCGACGCTTTCGTGTTCGGCCGCGGCGGCGAGGAG
GTCGACGCGCTGCGCCAGGCGAGCGTGTCCTACACGATCGTGCCCGGCATCACGGCGGGCATCGGCGCCGCCGCGCAGTT
CGAGGTGCCCTTGACCTTCCGCAGCAAGGCGCTGCGCATCACCTTCCTCACCGCGCACAAGGAGCGCGATGCCGAGGCGG
TCGACTGGTCGGCGCTGACCGATACCAAGATGACCGTCGTCGTCTACATGGGCATGACAGCGGCGCCGGCGGTGCGCTCC
GGCCTGCTCGCGGCGGGCCGCTCGCCGCAGACGCCGGTCGGCGTGTTCGCGCGCGTGACGCGCCCCGATGCGCAGGCCGC
GGTCGGTACGCTCGATCGATTGCCGGATCTCGTCGCCAAGATCGAGGGCGGTCCGGCCATCCTCATCATCGGTGATGTCG
TGGCGCATTCGGCGCCGTGGCGTCAGCAGACTCTCAGTGGCGTGATTGCAAAACTCCTGGAACCCGTCTGA

Upstream 100 bases:

>100_bases
TGGCGAAGCCAAGAAATAATTTTCTCTTTACGTCCGTAGCGTGACACTTATATAGAAAATAATTCCAGTCAACCCCGAAG
AGTCGAAGCCGAAGAGCGCA

Downstream 100 bases:

>100_bases
ATGACCTCCCCGCTGCAACAAAAGATCAAGATCACCGGTCCCTCGATCGTCACGGCGAACCGGACATGGGACGGCGCGGT
GATCTATCGCACCGGTTCGA

Product: siroheme synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MQYLPVFLDLKTGPVLLIGAGELVRAKLRVLLSAGARIRWFATDGDFDLGGLHDDHGQITRADGDPLTTDLSGVIAVLCA
GAGDIGVAMSARARSVGLPVNVMDDLAHSSFIFPAIVDRGDVVVAIGTGGSSPVVARRVRERIEALLPARIGELAGFIGD
FRKIINPRIAEMPLRRRFWERVVDGPIGALVLAGRKHDAEAALNSIADPTAFAGADHAGRGEGHVTLVGAGPGDPDLLTI
KALRALQDADVVFYDELVTPEILDRIRRDASRVPVGRRVGKPGIGQDAVNKLLIDAAREGKRAVRLKGGDAFVFGRGGEE
VDALRQASVSYTIVPGITAGIGAAAQFEVPLTFRSKALRITFLTAHKERDAEAVDWSALTDTKMTVVVYMGMTAAPAVRS
GLLAAGRSPQTPVGVFARVTRPDAQAAVGTLDRLPDLVAKIEGGPAILIIGDVVAHSAPWRQQTLSGVIAKLLEPV

Sequences:

>Translated_476_residues
MQYLPVFLDLKTGPVLLIGAGELVRAKLRVLLSAGARIRWFATDGDFDLGGLHDDHGQITRADGDPLTTDLSGVIAVLCA
GAGDIGVAMSARARSVGLPVNVMDDLAHSSFIFPAIVDRGDVVVAIGTGGSSPVVARRVRERIEALLPARIGELAGFIGD
FRKIINPRIAEMPLRRRFWERVVDGPIGALVLAGRKHDAEAALNSIADPTAFAGADHAGRGEGHVTLVGAGPGDPDLLTI
KALRALQDADVVFYDELVTPEILDRIRRDASRVPVGRRVGKPGIGQDAVNKLLIDAAREGKRAVRLKGGDAFVFGRGGEE
VDALRQASVSYTIVPGITAGIGAAAQFEVPLTFRSKALRITFLTAHKERDAEAVDWSALTDTKMTVVVYMGMTAAPAVRS
GLLAAGRSPQTPVGVFARVTRPDAQAAVGTLDRLPDLVAKIEGGPAILIIGDVVAHSAPWRQQTLSGVIAKLLEPV
>Mature_476_residues
MQYLPVFLDLKTGPVLLIGAGELVRAKLRVLLSAGARIRWFATDGDFDLGGLHDDHGQITRADGDPLTTDLSGVIAVLCA
GAGDIGVAMSARARSVGLPVNVMDDLAHSSFIFPAIVDRGDVVVAIGTGGSSPVVARRVRERIEALLPARIGELAGFIGD
FRKIINPRIAEMPLRRRFWERVVDGPIGALVLAGRKHDAEAALNSIADPTAFAGADHAGRGEGHVTLVGAGPGDPDLLTI
KALRALQDADVVFYDELVTPEILDRIRRDASRVPVGRRVGKPGIGQDAVNKLLIDAAREGKRAVRLKGGDAFVFGRGGEE
VDALRQASVSYTIVPGITAGIGAAAQFEVPLTFRSKALRITFLTAHKERDAEAVDWSALTDTKMTVVVYMGMTAAPAVRS
GLLAAGRSPQTPVGVFARVTRPDAQAAVGTLDRLPDLVAKIEGGPAILIIGDVVAHSAPWRQQTLSGVIAKLLEPV

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=460, Percent_Identity=38.0434782608696, Blast_Score=286, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6322922, Length=236, Percent_Identity=29.2372881355932, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 50197; Mature: 50197

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYLPVFLDLKTGPVLLIGAGELVRAKLRVLLSAGARIRWFATDGDFDLGGLHDDHGQIT
CCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEE
RADGDPLTTDLSGVIAVLCAGAGDIGVAMSARARSVGLPVNVMDDLAHSSFIFPAIVDRG
ECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCHHHCCCCHHHHHHHHHCCEEHHHHCCCC
DVVVAIGTGGSSPVVARRVRERIEALLPARIGELAGFIGDFRKIINPRIAEMPLRRRFWE
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHH
RVVDGPIGALVLAGRKHDAEAALNSIADPTAFAGADHAGRGEGHVTLVGAGPGDPDLLTI
HHHCCCCCEEEEECCCCCHHHHHHHCCCCCHHCCCCCCCCCCCEEEEEECCCCCCCEEHH
KALRALQDADVVFYDELVTPEILDRIRRDASRVPVGRRVGKPGIGQDAVNKLLIDAAREG
HHHHHHHCCCEEEEHHCCCHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHCC
KRAVRLKGGDAFVFGRGGEEVDALRQASVSYTIVPGITAGIGAAAQFEVPLTFRSKALRI
CEEEEEECCCEEEECCCCHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECEEECCCEEEE
TFLTAHKERDAEAVDWSALTDTKMTVVVYMGMTAAPAVRSGLLAAGRSPQTPVGVFARVT
EEEEECCCCCCCCCCCHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCHHEEEEEC
RPDAQAAVGTLDRLPDLVAKIEGGPAILIIGDVVAHSAPWRQQTLSGVIAKLLEPV
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQYLPVFLDLKTGPVLLIGAGELVRAKLRVLLSAGARIRWFATDGDFDLGGLHDDHGQIT
CCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEE
RADGDPLTTDLSGVIAVLCAGAGDIGVAMSARARSVGLPVNVMDDLAHSSFIFPAIVDRG
ECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCHHHCCCCHHHHHHHHHCCEEHHHHCCCC
DVVVAIGTGGSSPVVARRVRERIEALLPARIGELAGFIGDFRKIINPRIAEMPLRRRFWE
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHH
RVVDGPIGALVLAGRKHDAEAALNSIADPTAFAGADHAGRGEGHVTLVGAGPGDPDLLTI
HHHCCCCCEEEEECCCCCHHHHHHHCCCCCHHCCCCCCCCCCCEEEEEECCCCCCCEEHH
KALRALQDADVVFYDELVTPEILDRIRRDASRVPVGRRVGKPGIGQDAVNKLLIDAAREG
HHHHHHHCCCEEEEHHCCCHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHCC
KRAVRLKGGDAFVFGRGGEEVDALRQASVSYTIVPGITAGIGAAAQFEVPLTFRSKALRI
CEEEEEECCCEEEECCCCHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECEEECCCEEEE
TFLTAHKERDAEAVDWSALTDTKMTVVVYMGMTAAPAVRSGLLAAGRSPQTPVGVFARVT
EEEEECCCCCCCCCCCHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCHHEEEEEC
RPDAQAAVGTLDRLPDLVAKIEGGPAILIIGDVVAHSAPWRQQTLSGVIAKLLEPV
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA