Click here to switch to the map view.

The map label for this gene is cysD [H]

Identifier: 146338166

GI number: 146338166

Start: 1131719

End: 1132534

Strand: Reverse

Name: cysD [H]

Synonym: BRADO1064

Alternate gene names: 146338166

Gene position: 1132534-1131719 (Counterclockwise)

Preceding gene: 146338180

Following gene: 146338165

Centisome position: 15.19

GC content: 65.81

Gene sequence:

>816_bases
GTGCTGAGCGCCGCGCCGACGATGGATCATCTCGACCAGCTTGAGGCGCAGAGCATTTATATTCTCCGCGAGGCCTTTGC
ACGGCTGAAGAAGCTCGCGCTGCTGTGGTCGCTGGGAAAAGATTCCAACGTCATGATCTGGCTGGCGCGCAAGGCGTTCT
TCGGCCGCGTGCCGTTCCCGGCGATGCATGTCGATACCGGCAAGAAGTTTCCGGAGATGTATGCCTTCCGCGACACTTAC
GCGCAGGAATGGGGCCTCGATCTCCGCGTCGAGCCCTGCCCGCCGATCGATGCCGTCGATCCGACCCTGCCTCCGGCCGC
CCGCTCGGCCGCGCGCAAGACCGAGGGTCTGAAGATGGCGCTCGCGAAATACGGCTTCGACGGCCTGATCGCCGGCATCC
GCCGTGACGAGGAGGCGACGCGCGCCAAGGAGCGCGTGTTCTCGCCGCGCGGCGCCGAAGGCCATTGGGACGTCCGCGAC
CAGCCGCCGGAGTTCTGGGACCATTTCAACGCCTCGCCGCCGCAGGGCGCGCATTTGCGTATCCATCCGATCCTGCATTG
GACCGAGGCCGACATCTGGGCCTACACCGCGCGCGAGAACATTCCGATCATCCCGCTGTATCTGTCGAAGAACGGCAAGC
GCTATCGCTCGCTGGGCGATCAGGACATCACCTTCCCCGTCGCCTCCAACGCCTCGAGCATCGCGGAGATTCTGGCCGAG
CTCGAAAGCACCAAGGTGCCGGAGCGCGCCGGCCGCGCGCTCGACCACGAGACCGAGGACGCCTTCGAGCGTCTGCGCGT
CGCCGGCTATCTCTGA

Upstream 100 bases:

>100_bases
CAAGCAGGCGATGGCTGATAGCATGCACATTCTCACAAACGACGTTTCCAGAGATCGGTTGCCCTCCTCCGAGCTCGAGC
AAGCATCTTCCATGCCGCGC

Downstream 100 bases:

>100_bases
AGCGGAGCGCGAGGGTCATGAACATGAGAGTCCCGGCCGCCGCTCCCGCCACGCCGAACGGCACCAGCCGCCCGCAGGTC
TCAATCGTCATCGTCGGCCA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MLSAAPTMDHLDQLEAQSIYILREAFARLKKLALLWSLGKDSNVMIWLARKAFFGRVPFPAMHVDTGKKFPEMYAFRDTY
AQEWGLDLRVEPCPPIDAVDPTLPPAARSAARKTEGLKMALAKYGFDGLIAGIRRDEEATRAKERVFSPRGAEGHWDVRD
QPPEFWDHFNASPPQGAHLRIHPILHWTEADIWAYTARENIPIIPLYLSKNGKRYRSLGDQDITFPVASNASSIAEILAE
LESTKVPERAGRALDHETEDAFERLRVAGYL

Sequences:

>Translated_271_residues
MLSAAPTMDHLDQLEAQSIYILREAFARLKKLALLWSLGKDSNVMIWLARKAFFGRVPFPAMHVDTGKKFPEMYAFRDTY
AQEWGLDLRVEPCPPIDAVDPTLPPAARSAARKTEGLKMALAKYGFDGLIAGIRRDEEATRAKERVFSPRGAEGHWDVRD
QPPEFWDHFNASPPQGAHLRIHPILHWTEADIWAYTARENIPIIPLYLSKNGKRYRSLGDQDITFPVASNASSIAEILAE
LESTKVPERAGRALDHETEDAFERLRVAGYL
>Mature_271_residues
MLSAAPTMDHLDQLEAQSIYILREAFARLKKLALLWSLGKDSNVMIWLARKAFFGRVPFPAMHVDTGKKFPEMYAFRDTY
AQEWGLDLRVEPCPPIDAVDPTLPPAARSAARKTEGLKMALAKYGFDGLIAGIRRDEEATRAKERVFSPRGAEGHWDVRD
QPPEFWDHFNASPPQGAHLRIHPILHWTEADIWAYTARENIPIIPLYLSKNGKRYRSLGDQDITFPVASNASSIAEILAE
LESTKVPERAGRALDHETEDAFERLRVAGYL

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=299, Percent_Identity=41.1371237458194, Blast_Score=218, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 30637; Mature: 30637

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAAPTMDHLDQLEAQSIYILREAFARLKKLALLWSLGKDSNVMIWLARKAFFGRVPFP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCC
AMHVDTGKKFPEMYAFRDTYAQEWGLDLRVEPCPPIDAVDPTLPPAARSAARKTEGLKMA
EEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LAKYGFDGLIAGIRRDEEATRAKERVFSPRGAEGHWDVRDQPPEFWDHFNASPPQGAHLR
HHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEE
IHPILHWTEADIWAYTARENIPIIPLYLSKNGKRYRSLGDQDITFPVASNASSIAEILAE
EEEEEEEECCCEEEEECCCCCCEEEEEECCCCHHHHHCCCCCEEEECCCCHHHHHHHHHH
LESTKVPERAGRALDHETEDAFERLRVAGYL
HHHCCCCHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSAAPTMDHLDQLEAQSIYILREAFARLKKLALLWSLGKDSNVMIWLARKAFFGRVPFP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCC
AMHVDTGKKFPEMYAFRDTYAQEWGLDLRVEPCPPIDAVDPTLPPAARSAARKTEGLKMA
EEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LAKYGFDGLIAGIRRDEEATRAKERVFSPRGAEGHWDVRDQPPEFWDHFNASPPQGAHLR
HHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEE
IHPILHWTEADIWAYTARENIPIIPLYLSKNGKRYRSLGDQDITFPVASNASSIAEILAE
EEEEEEEECCCEEEEECCCCCCEEEEEECCCCHHHHHCCCCCEEEECCCCHHHHHHHHHH
LESTKVPERAGRALDHETEDAFERLRVAGYL
HHHCCCCHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA