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The map label for this gene is kdsB [H]

Identifier: 146338104

GI number: 146338104

Start: 1065496

End: 1066266

Strand: Reverse

Name: kdsB [H]

Synonym: BRADO1000

Alternate gene names: 146338104

Gene position: 1066266-1065496 (Counterclockwise)

Preceding gene: 146338111

Following gene: 146338103

Centisome position: 14.3

GC content: 69.13

Gene sequence:

>771_bases
TTGCGACCGGCTCCGACCTGCCGTTTTCGGATGACCCGATCCAAGATCCTCGTGCTGATCCCGGCCCGCATGGCCTCCAC
CCGCCTGCCCGGCAAGCCACTGCTCGACATCGCGGGCGTGCCGATGATCGTGCAGGTGTTGCGCCGTGCGGAAGAAGCGG
CGATCGGTCGCGTGGCGGTGGCGACCGACACGCCGGAGATCGCCGCGGCGGTGACCGCGGCGGGCGGCGAGGTGGTGATG
ACGAGGCCGGATCATCCCTCCGGGTCCGATCGCATCTACGAGGCGCTGTGCAAGCTCGATCCCGCTGGTGAGGCCGATTT
CGTGGTCAACCTGCAGGGCGACTTCCCGACCATCGACCCCAGGAGCATCAGCGACGTGCTGCCGCCGCTGGACGATCCCG
CCGTCGACATCGCCACGCTCGCCGCACAGATCCACACCGAAGAAGAGAGCCTCAATCCGAACGTGGTCAAGGCCGTCGGC
TCGCCCCTGGGTGGACGGCGCATGCGGGCGCTGTATTTCACCCGGGCCACGGCGCCGCATGGCGACGGGCCGCGCTACCA
CCACATCGGTCTCTATGCCTACCGTCGCGCGGCGCTGGAGCGCTTCGTGCGCCTGCCGCCGTCGCCACTCGAACAGCAGG
AGAAGCTGGAACAGCTGCGGGCGCTGGAGGCCGGCATGCGCATCGACGTCATGGTCGTCGATGCCGTCCCGCGCGGCGTG
GATACGCCGGCCGACCTCGAAACCGCCCGCCGGCTGCTGTCCAAAGCTTGA

Upstream 100 bases:

>100_bases
CCTCCCCCTGCGGCAGAAGTGAGATATCGGTTTGCCCGTGCTCTGGCAACCCGTATAAGCGGGCGGTTTTTGCTGTCCCC
ATCCCCGTCCGGACGCGCTG

Downstream 100 bases:

>100_bases
GCTGCTAGAAGGCCCATCATGAGCAAGATCTTGAAAATCGCATTCCAGGGCGAGCCGGGAGCCAACTCCCACATCGCCAT
TTCCGAGGCCTATCCGACTG

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRPAPTCRFRMTRSKILVLIPARMASTRLPGKPLLDIAGVPMIVQVLRRAEEAAIGRVAVATDTPEIAAAVTAAGGEVVM
TRPDHPSGSDRIYEALCKLDPAGEADFVVNLQGDFPTIDPRSISDVLPPLDDPAVDIATLAAQIHTEEESLNPNVVKAVG
SPLGGRRMRALYFTRATAPHGDGPRYHHIGLYAYRRAALERFVRLPPSPLEQQEKLEQLRALEAGMRIDVMVVDAVPRGV
DTPADLETARRLLSKA

Sequences:

>Translated_256_residues
MRPAPTCRFRMTRSKILVLIPARMASTRLPGKPLLDIAGVPMIVQVLRRAEEAAIGRVAVATDTPEIAAAVTAAGGEVVM
TRPDHPSGSDRIYEALCKLDPAGEADFVVNLQGDFPTIDPRSISDVLPPLDDPAVDIATLAAQIHTEEESLNPNVVKAVG
SPLGGRRMRALYFTRATAPHGDGPRYHHIGLYAYRRAALERFVRLPPSPLEQQEKLEQLRALEAGMRIDVMVVDAVPRGV
DTPADLETARRLLSKA
>Mature_256_residues
MRPAPTCRFRMTRSKILVLIPARMASTRLPGKPLLDIAGVPMIVQVLRRAEEAAIGRVAVATDTPEIAAAVTAAGGEVVM
TRPDHPSGSDRIYEALCKLDPAGEADFVVNLQGDFPTIDPRSISDVLPPLDDPAVDIATLAAQIHTEEESLNPNVVKAVG
SPLGGRRMRALYFTRATAPHGDGPRYHHIGLYAYRRAALERFVRLPPSPLEQQEKLEQLRALEAGMRIDVMVVDAVPRGV
DTPADLETARRLLSKA

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria [H]

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family [H]

Homologues:

Organism=Escherichia coli, GI1787147, Length=249, Percent_Identity=48.5943775100402, Blast_Score=196, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003329
- InterPro:   IPR004528 [H]

Pfam domain/function: PF02348 CTP_transf_3 [H]

EC number: =2.7.7.38 [H]

Molecular weight: Translated: 27773; Mature: 27773

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPAPTCRFRMTRSKILVLIPARMASTRLPGKPLLDIAGVPMIVQVLRRAEEAAIGRVAV
CCCCCCCEEEECCCEEEEEECCHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCEEEE
ATDTPEIAAAVTAAGGEVVMTRPDHPSGSDRIYEALCKLDPAGEADFVVNLQGDFPTIDP
ECCCCHHHHHEEECCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCC
RSISDVLPPLDDPAVDIATLAAQIHTEEESLNPNVVKAVGSPLGGRRMRALYFTRATAPH
CCHHHHCCCCCCCHHHHHHHHHHHHCCHHHCCCHHHHHHCCCCCCCCEEEEEEEECCCCC
GDGPRYHHIGLYAYRRAALERFVRLPPSPLEQQEKLEQLRALEAGMRIDVMVVDAVPRGV
CCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
DTPADLETARRLLSKA
CCCCCHHHHHHHHHCC
>Mature Secondary Structure
MRPAPTCRFRMTRSKILVLIPARMASTRLPGKPLLDIAGVPMIVQVLRRAEEAAIGRVAV
CCCCCCCEEEECCCEEEEEECCHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCEEEE
ATDTPEIAAAVTAAGGEVVMTRPDHPSGSDRIYEALCKLDPAGEADFVVNLQGDFPTIDP
ECCCCHHHHHEEECCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCC
RSISDVLPPLDDPAVDIATLAAQIHTEEESLNPNVVKAVGSPLGGRRMRALYFTRATAPH
CCHHHHCCCCCCCHHHHHHHHHHHHCCHHHCCCHHHHHHCCCCCCCCEEEEEEEECCCCC
GDGPRYHHIGLYAYRRAALERFVRLPPSPLEQQEKLEQLRALEAGMRIDVMVVDAVPRGV
CCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
DTPADLETARRLLSKA
CCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA