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The map label for this gene is metF [H]

Identifier: 146338099

GI number: 146338099

Start: 1060265

End: 1061182

Strand: Reverse

Name: metF [H]

Synonym: BRADO0995

Alternate gene names: 146338099

Gene position: 1061182-1060265 (Counterclockwise)

Preceding gene: 146338101

Following gene: 146338098

Centisome position: 14.23

GC content: 66.01

Gene sequence:

>918_bases
ATGACCGACCTCGCCACCGCCGTCACTGACGGACATTCCGCGATCATCCGGCCGAAGATCTCCTTCGAGTTCTTTCCGCC
GAAGACCGAGGAAATGGACCGGACGCTGTGGAGTTCGATCGAGCGGCTGGCGCCGCTGGAGCCGAGCTTCGTCTCCGTCA
CCTACGGCGCCGGCGGCTCGACCCGCGAGCGCACCCACGCCACTATCGCGCGCATCCTGAAAGAAACCGCGCTGCTGCCG
GCGGCGCATCTGACCTGCGTCGGCGCCTCCCGCCAGGAGATCGACGAGGTTGTCGATCGCTATCACGAGGTCGGCGTCCG
CCACATCGTGGCTCTGCGCGGCGATCCGCCAGGCGGCATCGGCACGCCCTATCACACCCACGCCGAGGGCTATCAGACCT
CGCCCGAACTGATCGCCGGCATCAAGAAGCGCCATCCGGATGTTGAGATCTCGGTCTCGGCCTATCCGGAGAAGCACCCG
GAGAGCCGCGACTTCGACGCCGACATCGACATGCTCAAGGCCAAGGTCGACGCCGGCGCGACGCGGGCCATCACGCAGGT
CTTCTTCGACAACGACCTCTACTTCCGCTACCTCGACCGGGTCCGCGCCCGCGGCATCGACATTCCGATCGTGCCCGGGA
TCATGCCGATGCACAATTTCAAGCAGGCGCGCGGCTTCGTCACCCGCGCCGGCACCACCGTGCCGGACTGGCTGGCCGAG
AAGTTCGACGGCCTCGACAACGATCCTGAGACCCGCAAGCTGGTGGCGGCGACCGTCGCCGCCGGCCAGGTGCACAAGCT
CGCCAAGCATGGCGTCGACACCTTCCATTTCTACACCATGAACCGCGCCGACCTCGTGTTCGCGATCAGCCATCTGCTTG
GCATCCGTCCGAAGTCCGTCAACAAGGTCGCCGCCTGA

Upstream 100 bases:

>100_bases
GTGGGCTGTTTATTGCGACCCACTTCCCCTATCTTGGCGCATCTGAGCAGCGATCCGCTCTCAAGTTGAAATTTACGCGC
CATCGGGAACCTGCGCCGCC

Downstream 100 bases:

>100_bases
TGTCCTCCCCTGTGTCCACCGCGCGGTCCGCTTTCCTCGCTGCAGCAGCCAAGCGCATCCTGATCCTCGACGGTGCCATG
GGCACGATGATCCAGGCGTT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTDLATAVTDGHSAIIRPKISFEFFPPKTEEMDRTLWSSIERLAPLEPSFVSVTYGAGGSTRERTHATIARILKETALLP
AAHLTCVGASRQEIDEVVDRYHEVGVRHIVALRGDPPGGIGTPYHTHAEGYQTSPELIAGIKKRHPDVEISVSAYPEKHP
ESRDFDADIDMLKAKVDAGATRAITQVFFDNDLYFRYLDRVRARGIDIPIVPGIMPMHNFKQARGFVTRAGTTVPDWLAE
KFDGLDNDPETRKLVAATVAAGQVHKLAKHGVDTFHFYTMNRADLVFAISHLLGIRPKSVNKVAA

Sequences:

>Translated_305_residues
MTDLATAVTDGHSAIIRPKISFEFFPPKTEEMDRTLWSSIERLAPLEPSFVSVTYGAGGSTRERTHATIARILKETALLP
AAHLTCVGASRQEIDEVVDRYHEVGVRHIVALRGDPPGGIGTPYHTHAEGYQTSPELIAGIKKRHPDVEISVSAYPEKHP
ESRDFDADIDMLKAKVDAGATRAITQVFFDNDLYFRYLDRVRARGIDIPIVPGIMPMHNFKQARGFVTRAGTTVPDWLAE
KFDGLDNDPETRKLVAATVAAGQVHKLAKHGVDTFHFYTMNRADLVFAISHLLGIRPKSVNKVAA
>Mature_304_residues
TDLATAVTDGHSAIIRPKISFEFFPPKTEEMDRTLWSSIERLAPLEPSFVSVTYGAGGSTRERTHATIARILKETALLPA
AHLTCVGASRQEIDEVVDRYHEVGVRHIVALRGDPPGGIGTPYHTHAEGYQTSPELIAGIKKRHPDVEISVSAYPEKHPE
SRDFDADIDMLKAKVDAGATRAITQVFFDNDLYFRYLDRVRARGIDIPIVPGIMPMHNFKQARGFVTRAGTTVPDWLAEK
FDGLDNDPETRKLVAATVAAGQVHKLAKHGVDTFHFYTMNRADLVFAISHLLGIRPKSVNKVAA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=285, Percent_Identity=33.6842105263158, Blast_Score=148, Evalue=5e-36,
Organism=Escherichia coli, GI1790377, Length=279, Percent_Identity=51.2544802867383, Blast_Score=292, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI25147436, Length=284, Percent_Identity=26.7605633802817, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI25147441, Length=284, Percent_Identity=26.7605633802817, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6325234, Length=307, Percent_Identity=32.2475570032573, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6321313, Length=289, Percent_Identity=30.4498269896194, Blast_Score=139, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24662658, Length=270, Percent_Identity=27.4074074074074, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33703; Mature: 33572

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLATAVTDGHSAIIRPKISFEFFPPKTEEMDRTLWSSIERLAPLEPSFVSVTYGAGGS
CCCHHHHHCCCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
TRERTHATIARILKETALLPAAHLTCVGASRQEIDEVVDRYHEVGVRHIVALRGDPPGGI
CHHHHHHHHHHHHHHHHHCCHHHHEEECCCHHHHHHHHHHHHHCCHHEEEEEECCCCCCC
GTPYHTHAEGYQTSPELIAGIKKRHPDVEISVSAYPEKHPESRDFDADIDMLKAKVDAGA
CCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCH
TRAITQVFFDNDLYFRYLDRVRARGIDIPIVPGIMPMHNFKQARGFVTRAGTTVPDWLAE
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHH
KFDGLDNDPETRKLVAATVAAGQVHKLAKHGVDTFHFYTMNRADLVFAISHLLGIRPKSV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCC
NKVAA
HHCCC
>Mature Secondary Structure 
TDLATAVTDGHSAIIRPKISFEFFPPKTEEMDRTLWSSIERLAPLEPSFVSVTYGAGGS
CCHHHHHCCCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
TRERTHATIARILKETALLPAAHLTCVGASRQEIDEVVDRYHEVGVRHIVALRGDPPGGI
CHHHHHHHHHHHHHHHHHCCHHHHEEECCCHHHHHHHHHHHHHCCHHEEEEEECCCCCCC
GTPYHTHAEGYQTSPELIAGIKKRHPDVEISVSAYPEKHPESRDFDADIDMLKAKVDAGA
CCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCH
TRAITQVFFDNDLYFRYLDRVRARGIDIPIVPGIMPMHNFKQARGFVTRAGTTVPDWLAE
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHH
KFDGLDNDPETRKLVAATVAAGQVHKLAKHGVDTFHFYTMNRADLVFAISHLLGIRPKSV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCC
NKVAA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA