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The map label for this gene is pcaR [H]

Identifier: 146338084

GI number: 146338084

Start: 1038766

End: 1039731

Strand: Reverse

Name: pcaR [H]

Synonym: BRADO0980

Alternate gene names: 146338084

Gene position: 1039731-1038766 (Counterclockwise)

Preceding gene: 146338092

Following gene: 146338083

Centisome position: 13.94

GC content: 62.84

Gene sequence:

>966_bases
ATGAAACGCCCAGGGAAGAAACCCGCGACCGATCGCAACTTCGTGGTTGCGCTGTCACGCGGCCTCGAGGTGCTCAGGGC
TTTCCGGCCCAATGACGGCCTGCTGGGCAACCAGGAGATCGCGGCACGCACCAATCTGCCCAAGGCCACGGTGTCGCGAC
TGACCTATACGCTGACCAAGCTTGGTTATCTCACCCCAGTTCCTCGTTTCGAGAAATATCAATTGGCGCCATCAGCCCTG
CTGCTCGGCTACGCCGCGCTCGCCAATCTCGGCGTGCGCCAACTCTCCGAACCGTTCCGCGAGCAGTTGATGCGCGAGAC
CGGCGGCGCGGTGGCGATCGGCGGCCGCGACCGGCTCAGCATGATCTATATCGGGCAGAGCCGTCACGGTGTCGTCAACG
TCGAACTCGACGTCGGCTCACGCATTCCGATCGCCACCACCGCCATGGGCCGGGCCTATCTTTGGGCGTTGCCCGAGGAG
GAACGCACCGCGCTGATGCGCGAGATGCGCGAGCATTACGGCAACCGCTGGCCGAAAATCCGCGACGGCATCGAGCGCGC
CGGCGAGACCGTCGCCAGGCACGGCTTCACCATCTCGGCCGGCGAATGGCAGGACGACGTCCACGCCGTCGGCGCGGCGC
TGAGGCTCAACGATGGAACGGGTCCCTATGCGATCAATTGCGGCGCGCCCGCATTCCGCTTCACGGAAGAGCGTCTGCTG
ACCGACATTGGACCTCGCCTCGTCGCGATGGTAAGAAAAATCGAAGCAGCCTTGGCGGACTCGCGCCGCAAACTAATCAG
ACCAATAATCAGACGAGCCAATCGTCTCACAAAGACCTCAATAAAAAGCCGAAGCCAGGAGGAAAAGTTGCCCGTGTTGC
CGAGGGTATCAGATAGCCTGCATCATCGCGGTCGGCTGGCGCCGCGCCTTCACCTGAACGATTTCGGGAGGGGCGAGACG
AGATGA

Upstream 100 bases:

>100_bases
TCAATTCCGCAGTGCGAAACGTAATTCCACATCTTGACAGCCAGGGTTATTTTGAAGCACGAGCGTTGTCAACGAGGCTC
ATCAATCAACAGCCGATGAC

Downstream 100 bases:

>100_bases
CGCAGGCACAACTCGCCCAGACCAACGACCCGCTGCTCGCGGTCCGCGATGTCAGCGTCGTGTTCGGCGGCATCGTGGCG
CTGAACGGCGTGTCGTTCGA

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKRPGKKPATDRNFVVALSRGLEVLRAFRPNDGLLGNQEIAARTNLPKATVSRLTYTLTKLGYLTPVPRFEKYQLAPSAL
LLGYAALANLGVRQLSEPFREQLMRETGGAVAIGGRDRLSMIYIGQSRHGVVNVELDVGSRIPIATTAMGRAYLWALPEE
ERTALMREMREHYGNRWPKIRDGIERAGETVARHGFTISAGEWQDDVHAVGAALRLNDGTGPYAINCGAPAFRFTEERLL
TDIGPRLVAMVRKIEAALADSRRKLIRPIIRRANRLTKTSIKSRSQEEKLPVLPRVSDSLHHRGRLAPRLHLNDFGRGET
R

Sequences:

>Translated_321_residues
MKRPGKKPATDRNFVVALSRGLEVLRAFRPNDGLLGNQEIAARTNLPKATVSRLTYTLTKLGYLTPVPRFEKYQLAPSAL
LLGYAALANLGVRQLSEPFREQLMRETGGAVAIGGRDRLSMIYIGQSRHGVVNVELDVGSRIPIATTAMGRAYLWALPEE
ERTALMREMREHYGNRWPKIRDGIERAGETVARHGFTISAGEWQDDVHAVGAALRLNDGTGPYAINCGAPAFRFTEERLL
TDIGPRLVAMVRKIEAALADSRRKLIRPIIRRANRLTKTSIKSRSQEEKLPVLPRVSDSLHHRGRLAPRLHLNDFGRGET
R
>Mature_321_residues
MKRPGKKPATDRNFVVALSRGLEVLRAFRPNDGLLGNQEIAARTNLPKATVSRLTYTLTKLGYLTPVPRFEKYQLAPSAL
LLGYAALANLGVRQLSEPFREQLMRETGGAVAIGGRDRLSMIYIGQSRHGVVNVELDVGSRIPIATTAMGRAYLWALPEE
ERTALMREMREHYGNRWPKIRDGIERAGETVARHGFTISAGEWQDDVHAVGAALRLNDGTGPYAINCGAPAFRFTEERLL
TDIGPRLVAMVRKIEAALADSRRKLIRPIIRRANRLTKTSIKSRSQEEKLPVLPRVSDSLHHRGRLAPRLHLNDFGRGET
R

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786715, Length=262, Percent_Identity=24.0458015267176, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87082359, Length=272, Percent_Identity=23.5294117647059, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 35841; Mature: 35841

Theoretical pI: Translated: 11.20; Mature: 11.20

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPGKKPATDRNFVVALSRGLEVLRAFRPNDGLLGNQEIAARTNLPKATVSRLTYTLTK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
LGYLTPVPRFEKYQLAPSALLLGYAALANLGVRQLSEPFREQLMRETGGAVAIGGRDRLS
HHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEE
MIYIGQSRHGVVNVELDVGSRIPIATTAMGRAYLWALPEEERTALMREMREHYGNRWPKI
EEEEECCCCCEEEEEEECCCCCCCHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCHH
RDGIERAGETVARHGFTISAGEWQDDVHAVGAALRLNDGTGPYAINCGAPAFRFTEERLL
HHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHEEEECCCCCCEEEECCCCCHHHHHHHHH
TDIGPRLVAMVRKIEAALADSRRKLIRPIIRRANRLTKTSIKSRSQEEKLPVLPRVSDSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHH
HHRGRLAPRLHLNDFGRGETR
HHCCCCCCEEECCCCCCCCCC
>Mature Secondary Structure
MKRPGKKPATDRNFVVALSRGLEVLRAFRPNDGLLGNQEIAARTNLPKATVSRLTYTLTK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
LGYLTPVPRFEKYQLAPSALLLGYAALANLGVRQLSEPFREQLMRETGGAVAIGGRDRLS
HHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEE
MIYIGQSRHGVVNVELDVGSRIPIATTAMGRAYLWALPEEERTALMREMREHYGNRWPKI
EEEEECCCCCEEEEEEECCCCCCCHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCHH
RDGIERAGETVARHGFTISAGEWQDDVHAVGAALRLNDGTGPYAINCGAPAFRFTEERLL
HHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHEEEECCCCCCEEEECCCCCHHHHHHHHH
TDIGPRLVAMVRKIEAALADSRRKLIRPIIRRANRLTKTSIKSRSQEEKLPVLPRVSDSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHH
HHRGRLAPRLHLNDFGRGETR
HHCCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]