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The map label for this gene is braD [H]

Identifier: 146338075

GI number: 146338075

Start: 1029397

End: 1030422

Strand: Reverse

Name: braD [H]

Synonym: BRADO0971

Alternate gene names: 146338075

Gene position: 1030422-1029397 (Counterclockwise)

Preceding gene: 146338077

Following gene: 146338074

Centisome position: 13.82

GC content: 66.08

Gene sequence:

>1026_bases
TTGCTGTTCCTGTTGCAGGACGGCATTACCAACGGCGCGATCTACGCCCTGCTCGGGCTGGCGCTGGTGCTGGTATTCGC
CGTCACGCGCGTCATCCTCATCCCGCAGGGCGAATTCGTCACCTACGGCGCGCTCACCTACGCCTCGCTTGCCGCCGGCC
GCATGCCCGGGACCGCCAAGCTTGCGGTCGTGATCGGCCTCGTCGCCTTTGCCTTTGACCTCTATGCGATGCGCAAGACG
CTGCACGCGCCAAAGATCGCGCGCGCCTTCGCCCTTCACGTCGCCTTTCCGGTCGTCGTACTGCTCGCCAGCATGTGGGC
TGCCGACCAACGCATGCCAGCGGGCGTCAGCCTGGTGCTGTCGCTGATGATCGTGGCGAGCATCGGCCTGTCGCTGTACC
GCATCGTATTCCAGCCGCTCGCCCACACCTCGGTGCTGGTGCTGCTGATCGCCTCGGTCGGCGTCCACCTCGCGCTGCAG
GGCCTTGGCCTGTTGTTCTTCGGCGCCGAAGGGCAGCGCGGCCCGACGCTCCTGACCGCCAGCGCAACGCTCGGCTCGCT
CCGCTTCACCGGCCAGGGCATGGCGGTCTACGGCATCACCGTTGCGTTCATCATCGGCCTCTGGCTGTTCTTCGGCTTGA
CCTTGTACGGCAAGGCGCTGCGCGCCACCGCCGTCAACCGGCTCGGCGCCCGCCTGGTCGGCATCCGCACCACGCTGTCC
GGCCAGATCGCGTTCCTGCTGGCCTCCACCATCGGCGCGCTCTCCGGCATCCTGATCGTCCCGATCACGACGCTCTATTA
CGACACCGGCTTCCTGATCGGCCTGAAGGGCTTCATCGCCGCGATCATCGGCGGTCTCATCAGCTATCCGCTGACCGCCG
TGGCCGCGATCGTCGTTGGCATCGTCGAGGCGTTCTCCTCGTTCTACGCCTCGAACTTCAAGGAAGTGATCGTCTTCACA
CTGCTGATCCCGGTCCTGCTGCTGCGTTCGCTCGCCGCGCCCGCGGTCGAAGAAGAGAAGGATTGA

Upstream 100 bases:

>100_bases
CGGGCAGCCATTCAACTGCTCCGACCAGCCGCGGGCGCAAGGCACCCCGCGGCGTGCGGTCACCATTGAGGGGGAGCGTC
CTGTCTTGAACACAACGATC

Downstream 100 bases:

>100_bases
GCCGTGCGCGAGCGTTGGCCTCTCATCATCTTTGCCGTCCTCGTCGCGGCGATCCCGTTCATCCCGGGCATGCCGCCGTT
CTGGATCGTGCTGCTCGACA

Product: putative branched-chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MLFLLQDGITNGAIYALLGLALVLVFAVTRVILIPQGEFVTYGALTYASLAAGRMPGTAKLAVVIGLVAFAFDLYAMRKT
LHAPKIARAFALHVAFPVVVLLASMWAADQRMPAGVSLVLSLMIVASIGLSLYRIVFQPLAHTSVLVLLIASVGVHLALQ
GLGLLFFGAEGQRGPTLLTASATLGSLRFTGQGMAVYGITVAFIIGLWLFFGLTLYGKALRATAVNRLGARLVGIRTTLS
GQIAFLLASTIGALSGILIVPITTLYYDTGFLIGLKGFIAAIIGGLISYPLTAVAAIVVGIVEAFSSFYASNFKEVIVFT
LLIPVLLLRSLAAPAVEEEKD

Sequences:

>Translated_341_residues
MLFLLQDGITNGAIYALLGLALVLVFAVTRVILIPQGEFVTYGALTYASLAAGRMPGTAKLAVVIGLVAFAFDLYAMRKT
LHAPKIARAFALHVAFPVVVLLASMWAADQRMPAGVSLVLSLMIVASIGLSLYRIVFQPLAHTSVLVLLIASVGVHLALQ
GLGLLFFGAEGQRGPTLLTASATLGSLRFTGQGMAVYGITVAFIIGLWLFFGLTLYGKALRATAVNRLGARLVGIRTTLS
GQIAFLLASTIGALSGILIVPITTLYYDTGFLIGLKGFIAAIIGGLISYPLTAVAAIVVGIVEAFSSFYASNFKEVIVFT
LLIPVLLLRSLAAPAVEEEKD
>Mature_341_residues
MLFLLQDGITNGAIYALLGLALVLVFAVTRVILIPQGEFVTYGALTYASLAAGRMPGTAKLAVVIGLVAFAFDLYAMRKT
LHAPKIARAFALHVAFPVVVLLASMWAADQRMPAGVSLVLSLMIVASIGLSLYRIVFQPLAHTSVLVLLIASVGVHLALQ
GLGLLFFGAEGQRGPTLLTASATLGSLRFTGQGMAVYGITVAFIIGLWLFFGLTLYGKALRATAVNRLGARLVGIRTTLS
GQIAFLLASTIGALSGILIVPITTLYYDTGFLIGLKGFIAAIIGGLISYPLTAVAAIVVGIVEAFSSFYASNFKEVIVFT
LLIPVLLLRSLAAPAVEEEKD

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=263, Percent_Identity=26.9961977186312, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 35846; Mature: 35846

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFLLQDGITNGAIYALLGLALVLVFAVTRVILIPQGEFVTYGALTYASLAAGRMPGTAK
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCHHH
LAVVIGLVAFAFDLYAMRKTLHAPKIARAFALHVAFPVVVLLASMWAADQRMPAGVSLVL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
SLMIVASIGLSLYRIVFQPLAHTSVLVLLIASVGVHLALQGLGLLFFGAEGQRGPTLLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEE
SATLGSLRFTGQGMAVYGITVAFIIGLWLFFGLTLYGKALRATAVNRLGARLVGIRTTLS
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQIAFLLASTIGALSGILIVPITTLYYDTGFLIGLKGFIAAIIGGLISYPLTAVAAIVVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVEAFSSFYASNFKEVIVFTLLIPVLLLRSLAAPAVEEEKD
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLFLLQDGITNGAIYALLGLALVLVFAVTRVILIPQGEFVTYGALTYASLAAGRMPGTAK
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCHHH
LAVVIGLVAFAFDLYAMRKTLHAPKIARAFALHVAFPVVVLLASMWAADQRMPAGVSLVL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
SLMIVASIGLSLYRIVFQPLAHTSVLVLLIASVGVHLALQGLGLLFFGAEGQRGPTLLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEE
SATLGSLRFTGQGMAVYGITVAFIIGLWLFFGLTLYGKALRATAVNRLGARLVGIRTTLS
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQIAFLLASTIGALSGILIVPITTLYYDTGFLIGLKGFIAAIIGGLISYPLTAVAAIVVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVEAFSSFYASNFKEVIVFTLLIPVLLLRSLAAPAVEEEKD
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]