BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is Hadh [H]

Identifier: 146338069

GI number: 146338069

Start: 1023216

End: 1023980

Strand: Direct

Name: Hadh [H]

Synonym: BRADO0964

Alternate gene names: 146338069

Gene position: 1023216-1023980 (Clockwise)

Preceding gene: 146338068

Following gene: 146338070

Centisome position: 13.72

GC content: 69.15

Gene sequence:

>765_bases
ATGTTGTTGAAGGATCAGGCCGCCATCGTCACCGGCGGCGCCTCGGGCCTCGGCGCCGCCACCGCCCGCAAGCTCGCCTC
GCAGGGCGCCAAGGTCGCCGTGTTCGATCTCAATGCCAAGCTGGCGGAGGAGGTCGCGGCCGAGATCAAGGGCGTTCCGG
TGATCTGCGACGTCTCCGATGCCGCGGGCGCGGAAGCCGCCGTCGCCAAGGCGGTCGAGGCGCTCGGTCAGCAGCCGCGC
GTGCTGGTCAACTGCGCCGGCATCGGCGTCGCCAAGCGCGTCGTCGGCCGTGACGGCCCGATGGCGCTGTCGGATTTCGA
GAAGGTCATCAAGGTCAACCTGATCGGCTCGTTCAACATGCTGCGCCTGGCCACCAACGGCATGACCAAGCTGGAGCCGC
AGGCGACCGGCGAGCGCGGCGTCGTCATCAACACCGCGTCCGTCGCAGCCTATGACGGCCAGATCGGGCAGTCGGCCTAT
TCGGCGTCGAAGGGCGGCATCGTCGGTATGACCCTGCCGATCGCCCGCGAGCTCGCGCAGTTCGGCATCCGCGTGTTGAC
CATCGCGCCCGGCCTGTTCCTGACCCCGCTGCTCGCCAATTTGCCGCAGGAAGCCCAGGACTCGCTGGCCGCCGCGATCC
CGTTCCCCCGCCGCCTCGGCGATGCCTCCGAGTTCGCCGCGCTGGCGCTGCACATGGTCGAGAACCCCTATCTCAATGGC
GAAGTCGTCCGCCTCGACGGCTCGCTCCGCATGGCGCCGAAGTGA

Upstream 100 bases:

>100_bases
GCTGGAGAACCGCAAGGACCTGATCGAGCGCATCTACGCGTCGACACCGGACGACGACATCATCATCGCCGGCTGATCAA
CGAAATAAGGGAGAGACGAC

Downstream 100 bases:

>100_bases
GAATTCATCATTGCGAGGAGCGAAGCGACGAAGCAATCCAGAGTCCCGATGGGACCCTGGATTGCTTCGCTTCGCTCGCA
ATGACGGAGAGAAACGTAGG

Product: 3-hydroxyacyl-CoA dehydrogenase

Products: 3-oxoacyl-CoA; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLLKDQAAIVTGGASGLGAATARKLASQGAKVAVFDLNAKLAEEVAAEIKGVPVICDVSDAAGAEAAVAKAVEALGQQPR
VLVNCAGIGVAKRVVGRDGPMALSDFEKVIKVNLIGSFNMLRLATNGMTKLEPQATGERGVVINTASVAAYDGQIGQSAY
SASKGGIVGMTLPIARELAQFGIRVLTIAPGLFLTPLLANLPQEAQDSLAAAIPFPRRLGDASEFAALALHMVENPYLNG
EVVRLDGSLRMAPK

Sequences:

>Translated_254_residues
MLLKDQAAIVTGGASGLGAATARKLASQGAKVAVFDLNAKLAEEVAAEIKGVPVICDVSDAAGAEAAVAKAVEALGQQPR
VLVNCAGIGVAKRVVGRDGPMALSDFEKVIKVNLIGSFNMLRLATNGMTKLEPQATGERGVVINTASVAAYDGQIGQSAY
SASKGGIVGMTLPIARELAQFGIRVLTIAPGLFLTPLLANLPQEAQDSLAAAIPFPRRLGDASEFAALALHMVENPYLNG
EVVRLDGSLRMAPK
>Mature_254_residues
MLLKDQAAIVTGGASGLGAATARKLASQGAKVAVFDLNAKLAEEVAAEIKGVPVICDVSDAAGAEAAVAKAVEALGQQPR
VLVNCAGIGVAKRVVGRDGPMALSDFEKVIKVNLIGSFNMLRLATNGMTKLEPQATGERGVVINTASVAAYDGQIGQSAY
SASKGGIVGMTLPIARELAQFGIRVLTIAPGLFLTPLLANLPQEAQDSLAAAIPFPRRLGDASEFAALALHMVENPYLNG
EVVRLDGSLRMAPK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=255, Percent_Identity=51.7647058823529, Blast_Score=245, Evalue=3e-65,
Organism=Homo sapiens, GI83715985, Length=255, Percent_Identity=48.6274509803922, Blast_Score=221, Evalue=6e-58,
Organism=Homo sapiens, GI15277342, Length=263, Percent_Identity=32.319391634981, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI40254992, Length=252, Percent_Identity=32.5396825396825, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI31542939, Length=267, Percent_Identity=28.8389513108614, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI5031737, Length=263, Percent_Identity=28.5171102661597, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI33667109, Length=199, Percent_Identity=32.1608040201005, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI22507405, Length=228, Percent_Identity=29.8245614035088, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI224922801, Length=179, Percent_Identity=33.5195530726257, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI59889578, Length=224, Percent_Identity=26.3392857142857, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI32483357, Length=254, Percent_Identity=27.5590551181102, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4504505, Length=193, Percent_Identity=32.6424870466321, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI10190704, Length=257, Percent_Identity=26.8482490272374, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI150247226, Length=200, Percent_Identity=30, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=30.7692307692308, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787335, Length=258, Percent_Identity=30.6201550387597, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87082160, Length=255, Percent_Identity=32.5490196078431, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789057, Length=209, Percent_Identity=32.0574162679426, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1788459, Length=255, Percent_Identity=29.0196078431373, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI2367175, Length=253, Percent_Identity=31.2252964426877, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787905, Length=253, Percent_Identity=30.0395256916996, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789378, Length=266, Percent_Identity=28.9473684210526, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789208, Length=253, Percent_Identity=27.6679841897233, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1786812, Length=182, Percent_Identity=32.4175824175824, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790717, Length=254, Percent_Identity=30.7086614173228, Blast_Score=67, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17538182, Length=247, Percent_Identity=52.2267206477733, Blast_Score=225, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17555706, Length=255, Percent_Identity=33.7254901960784, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25147288, Length=256, Percent_Identity=32.421875, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560150, Length=262, Percent_Identity=25.9541984732824, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17562910, Length=266, Percent_Identity=25.5639097744361, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=199, Percent_Identity=30.1507537688442, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=262, Percent_Identity=25.1908396946565, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17531453, Length=208, Percent_Identity=30.2884615384615, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17538480, Length=201, Percent_Identity=29.8507462686567, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17567345, Length=203, Percent_Identity=28.0788177339901, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17551412, Length=216, Percent_Identity=28.2407407407407, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17507613, Length=206, Percent_Identity=32.0388349514563, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32563809, Length=206, Percent_Identity=32.0388349514563, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17562906, Length=200, Percent_Identity=29.5, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17568967, Length=221, Percent_Identity=28.5067873303167, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=259, Percent_Identity=27.027027027027, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17562990, Length=263, Percent_Identity=26.2357414448669, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17562908, Length=196, Percent_Identity=30.1020408163265, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17560220, Length=200, Percent_Identity=29.5, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17562904, Length=202, Percent_Identity=25.7425742574257, Blast_Score=64, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=200, Percent_Identity=28, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322861, Length=203, Percent_Identity=35.4679802955665, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320089, Length=214, Percent_Identity=26.1682242990654, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI17737361, Length=256, Percent_Identity=51.5625, Blast_Score=247, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24639444, Length=257, Percent_Identity=33.852140077821, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24644339, Length=205, Percent_Identity=31.219512195122, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221331218, Length=211, Percent_Identity=30.8056872037915, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24665243, Length=211, Percent_Identity=30.8056872037915, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221331216, Length=212, Percent_Identity=31.1320754716981, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24665247, Length=212, Percent_Identity=31.1320754716981, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI23397609, Length=236, Percent_Identity=30.9322033898305, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21357041, Length=211, Percent_Identity=30.3317535545024, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355319, Length=249, Percent_Identity=26.9076305220884, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571526, Length=200, Percent_Identity=31, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.35

Molecular weight: Translated: 26100; Mature: 26100

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKDQAAIVTGGASGLGAATARKLASQGAKVAVFDLNAKLAEEVAAEIKGVPVICDVSD
CCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCEEEEECCC
AAGAEAAVAKAVEALGQQPRVLVNCAGIGVAKRVVGRDGPMALSDFEKVIKVNLIGSFNM
CCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHEEEEECCCCE
LRLATNGMTKLEPQATGERGVVINTASVAAYDGQIGQSAYSASKGGIVGMTLPIARELAQ
EEEECCCCCCCCCCCCCCCCEEEEECEEEEECCCCCHHHHCCCCCCEEEEHHHHHHHHHH
FGIRVLTIAPGLFLTPLLANLPQEAQDSLAAAIPFPRRLGDASEFAALALHMVENPYLNG
CCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCCCC
EVVRLDGSLRMAPK
EEEEECCCEECCCC
>Mature Secondary Structure
MLLKDQAAIVTGGASGLGAATARKLASQGAKVAVFDLNAKLAEEVAAEIKGVPVICDVSD
CCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCEEEEECCC
AAGAEAAVAKAVEALGQQPRVLVNCAGIGVAKRVVGRDGPMALSDFEKVIKVNLIGSFNM
CCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHEEEEECCCCE
LRLATNGMTKLEPQATGERGVVINTASVAAYDGQIGQSAYSASKGGIVGMTLPIARELAQ
EEEECCCCCCCCCCCCCCCCEEEEECEEEEECCCCCHHHHCCCCCCEEEEHHHHHHHHHH
FGIRVLTIAPGLFLTPLLANLPQEAQDSLAAAIPFPRRLGDASEFAALALHMVENPYLNG
CCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCCCC
EVVRLDGSLRMAPK
EEEEECCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-3-hydroxyacyl-CoA; NAD+

Specific reaction: (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]