Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 146338029

GI number: 146338029

Start: 984832

End: 986301

Strand: Reverse

Name: gatA [H]

Synonym: BRADO0922

Alternate gene names: 146338029

Gene position: 986301-984832 (Counterclockwise)

Preceding gene: 146338030

Following gene: 146338026

Centisome position: 13.23

GC content: 68.84

Gene sequence:

>1470_bases
TTGGCCGCATCGCAATGGACGTTCGCAACCGCAGTCGACACAGCGAAGGCATTGGCGCGTCGCGAGATCTCGTCGGTCGA
GCTGACGCAGCTCGCGATCGATCGCATCATCCGACATGACGACAAGATCAACGCCATCTGCGTGTGCGATTTCGATCGCG
CACTGGCTGCCGCACGCGCCGCCGATGCGGCGCTTGCACGCGGCGAGCACGGGCCGCTGCTCGGCCTGCCACTGACGGTG
AAGGAGTCCTTCAACGTCGCCGGGCTGCCGACGACCTGGGGCTTTCCGCAGCAGAAAGACTTCATTGCCGCCGAGGATGC
GCTGACCGTGGCGCGCGTGAAGGACGCCGGCGGCATCGTGGTCGGCAAGACCAACGTGCCGATCGCGCTGGGCGACTGGC
AGAGCTACAACGACATCTACGGCACCACCAACAACCCCTACGACCTCGGCCGCACGCCCGGTGGCTCCTCCGGCGGCTCT
TCGGCAGCGCTCGCGGCGGGCTATGGCTCGCTCTCGCTCGGCTCCGACATCGGCGGCTCCTTGCGCGTGCCGGCCTTCCA
TTGCGGCGTGACCGCGCACAAGCCCACTTATGGTCTTGTCCCTACGCGCGGCCACACGCCGCCGCCATTCCCGCCGCTGC
CGAACGAGGCGGACCTTGCGGTGGTCGGCCCGATGGCGCGCTCGGCCGCCGATCTCGCGCTGCTGCTCGACGTGATGGCA
GGCCCCGATCCGCTGGAAGCCGGCAAAGCGTATCGGCTGGAGCTGCCGCCGGCGCGGCACACGCGGCTGCAGGACTTTCG
CGTGCTCGTCGTCGCCAGCCATCCGCTGCTTCCGACCGACACCGCCGTGCGCGGCGCGATCGAGCGGCTCGCAGCCAATC
TGGCGAAAGCGGGCGTCAGCATCGTCATGGAAAGCCCGCTGCTGCCCGATTTCGCCAGCTCCAGCCGGCTTTATATGCGC
CTGCTGCTCGGCTTCCTCGGCGCAACCATGCCGCCGGACGTTCATGCCGGCGCCATCCAGGCAGCCGCGCACCTGCCCAA
GGAGGCCTTAAGCCTCGGCGCCGAGCGTCTGCGCGGCATCGCGCTCAGCCACCGCGACTGGGTGCAGGCCAGCAACGCCC
GTTTCGGGCTGCGCGCGCAGTGGCGAGCTTTGTTCGAGAGCTTCGACGCGGTGATCTGCCCGATCATGCCGACACCCGCG
TTTCGGCACGACCACTCGCCTGACCAGACGCAGCGCCGCATCAGCATCGACGGCGTCGAGCATGACTACAATGACCAGCT
CGCCTGGCCTGGGGTCGCGACCCTGCCCGGCCTCCCCGCCACGCAGATTCCGCTCGGGCTCGTCGACGGCTTGCCGGTCG
GCGCGCAGATCGTCGGCCCGTTCCTCGAGGACCGCACGCCGCTGAAGCTGGCCGAACTGATCGAGCGCGAGTTCGGCGGC
TTCACGCCACCGCCGATGTTTGCGGATTAG

Upstream 100 bases:

>100_bases
GCGCATGCTGCCCAGCTAGTCTCAACCGCGCAATTTCGATCAAGCGCCTGCCATCGCCCTGAGGTAGAAACGGACCTGCA
CGCAAACAGGGAGCAGGACC

Downstream 100 bases:

>100_bases
TTCACGTCAGGAGCGCTGCCCACCACCGTCGTCCCGGCCTTGAGCCGGGACCCATAGCCACCGGCGCTTTGGGAACGATG
ACTCGTGATTGAGCTAACTC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MAASQWTFATAVDTAKALARREISSVELTQLAIDRIIRHDDKINAICVCDFDRALAAARAADAALARGEHGPLLGLPLTV
KESFNVAGLPTTWGFPQQKDFIAAEDALTVARVKDAGGIVVGKTNVPIALGDWQSYNDIYGTTNNPYDLGRTPGGSSGGS
SAALAAGYGSLSLGSDIGGSLRVPAFHCGVTAHKPTYGLVPTRGHTPPPFPPLPNEADLAVVGPMARSAADLALLLDVMA
GPDPLEAGKAYRLELPPARHTRLQDFRVLVVASHPLLPTDTAVRGAIERLAANLAKAGVSIVMESPLLPDFASSSRLYMR
LLLGFLGATMPPDVHAGAIQAAAHLPKEALSLGAERLRGIALSHRDWVQASNARFGLRAQWRALFESFDAVICPIMPTPA
FRHDHSPDQTQRRISIDGVEHDYNDQLAWPGVATLPGLPATQIPLGLVDGLPVGAQIVGPFLEDRTPLKLAELIEREFGG
FTPPPMFAD

Sequences:

>Translated_489_residues
MAASQWTFATAVDTAKALARREISSVELTQLAIDRIIRHDDKINAICVCDFDRALAAARAADAALARGEHGPLLGLPLTV
KESFNVAGLPTTWGFPQQKDFIAAEDALTVARVKDAGGIVVGKTNVPIALGDWQSYNDIYGTTNNPYDLGRTPGGSSGGS
SAALAAGYGSLSLGSDIGGSLRVPAFHCGVTAHKPTYGLVPTRGHTPPPFPPLPNEADLAVVGPMARSAADLALLLDVMA
GPDPLEAGKAYRLELPPARHTRLQDFRVLVVASHPLLPTDTAVRGAIERLAANLAKAGVSIVMESPLLPDFASSSRLYMR
LLLGFLGATMPPDVHAGAIQAAAHLPKEALSLGAERLRGIALSHRDWVQASNARFGLRAQWRALFESFDAVICPIMPTPA
FRHDHSPDQTQRRISIDGVEHDYNDQLAWPGVATLPGLPATQIPLGLVDGLPVGAQIVGPFLEDRTPLKLAELIEREFGG
FTPPPMFAD
>Mature_488_residues
AASQWTFATAVDTAKALARREISSVELTQLAIDRIIRHDDKINAICVCDFDRALAAARAADAALARGEHGPLLGLPLTVK
ESFNVAGLPTTWGFPQQKDFIAAEDALTVARVKDAGGIVVGKTNVPIALGDWQSYNDIYGTTNNPYDLGRTPGGSSGGSS
AALAAGYGSLSLGSDIGGSLRVPAFHCGVTAHKPTYGLVPTRGHTPPPFPPLPNEADLAVVGPMARSAADLALLLDVMAG
PDPLEAGKAYRLELPPARHTRLQDFRVLVVASHPLLPTDTAVRGAIERLAANLAKAGVSIVMESPLLPDFASSSRLYMRL
LLGFLGATMPPDVHAGAIQAAAHLPKEALSLGAERLRGIALSHRDWVQASNARFGLRAQWRALFESFDAVICPIMPTPAF
RHDHSPDQTQRRISIDGVEHDYNDQLAWPGVATLPGLPATQIPLGLVDGLPVGAQIVGPFLEDRTPLKLAELIEREFGGF
TPPPMFAD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=509, Percent_Identity=32.2200392927308, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI222831590, Length=514, Percent_Identity=25.4863813229572, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI166795287, Length=364, Percent_Identity=27.4725274725275, Blast_Score=73, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=502, Percent_Identity=29.0836653386454, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17538252, Length=419, Percent_Identity=28.4009546539379, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=257, Percent_Identity=33.0739299610895, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71990152, Length=252, Percent_Identity=32.1428571428571, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17556276, Length=414, Percent_Identity=30.1932367149758, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=414, Percent_Identity=30.1932367149758, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=429, Percent_Identity=26.3403263403263, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538254, Length=516, Percent_Identity=25, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320448, Length=253, Percent_Identity=29.6442687747036, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319685, Length=439, Percent_Identity=25.9681093394077, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648435, Length=499, Percent_Identity=31.8637274549098, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24648437, Length=499, Percent_Identity=31.8637274549098, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI45550774, Length=499, Percent_Identity=31.8637274549098, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24652985, Length=509, Percent_Identity=31.434184675835, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI19922090, Length=509, Percent_Identity=31.434184675835, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24652981, Length=509, Percent_Identity=31.434184675835, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24652983, Length=509, Percent_Identity=31.434184675835, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24644968, Length=519, Percent_Identity=28.5163776493256, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI161078093, Length=501, Percent_Identity=29.3413173652695, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24648441, Length=429, Percent_Identity=31.7016317016317, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24648439, Length=429, Percent_Identity=31.7016317016317, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21356731, Length=510, Percent_Identity=29.2156862745098, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24648113, Length=458, Percent_Identity=29.0393013100437, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 51843; Mature: 51712

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASQWTFATAVDTAKALARREISSVELTQLAIDRIIRHDDKINAICVCDFDRALAAARA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHH
ADAALARGEHGPLLGLPLTVKESFNVAGLPTTWGFPQQKDFIAAEDALTVARVKDAGGIV
HHHHHHCCCCCCEEECEEEECCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEEECCCCEE
VGKTNVPIALGDWQSYNDIYGTTNNPYDLGRTPGGSSGGSSAALAAGYGSLSLGSDIGGS
ECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCC
LRVPAFHCGVTAHKPTYGLVPTRGHTPPPFPPLPNEADLAVVGPMARSAADLALLLDVMA
EECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHC
GPDPLEAGKAYRLELPPARHTRLQDFRVLVVASHPLLPTDTAVRGAIERLAANLAKAGVS
CCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCE
IVMESPLLPDFASSSRLYMRLLLGFLGATMPPDVHAGAIQAAAHLPKEALSLGAERLRGI
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
ALSHRDWVQASNARFGLRAQWRALFESFDAVICPIMPTPAFRHDHSPDQTQRRISIDGVE
HCCCHHHHHCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHEEEECCCC
HDYNDQLAWPGVATLPGLPATQIPLGLVDGLPVGAQIVGPFLEDRTPLKLAELIEREFGG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCC
FTPPPMFAD
CCCCCCCCC
>Mature Secondary Structure 
AASQWTFATAVDTAKALARREISSVELTQLAIDRIIRHDDKINAICVCDFDRALAAARA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHH
ADAALARGEHGPLLGLPLTVKESFNVAGLPTTWGFPQQKDFIAAEDALTVARVKDAGGIV
HHHHHHCCCCCCEEECEEEECCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEEECCCCEE
VGKTNVPIALGDWQSYNDIYGTTNNPYDLGRTPGGSSGGSSAALAAGYGSLSLGSDIGGS
ECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCC
LRVPAFHCGVTAHKPTYGLVPTRGHTPPPFPPLPNEADLAVVGPMARSAADLALLLDVMA
EECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHC
GPDPLEAGKAYRLELPPARHTRLQDFRVLVVASHPLLPTDTAVRGAIERLAANLAKAGVS
CCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCE
IVMESPLLPDFASSSRLYMRLLLGFLGATMPPDVHAGAIQAAAHLPKEALSLGAERLRGI
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
ALSHRDWVQASNARFGLRAQWRALFESFDAVICPIMPTPAFRHDHSPDQTQRRISIDGVE
HCCCHHHHHCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHEEEECCCC
HDYNDQLAWPGVATLPGLPATQIPLGLVDGLPVGAQIVGPFLEDRTPLKLAELIEREFGG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCC
FTPPPMFAD
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA