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The map label for this gene is gatA [H]

Identifier: 146338015

GI number: 146338015

Start: 968351

End: 969763

Strand: Direct

Name: gatA [H]

Synonym: BRADO0908

Alternate gene names: 146338015

Gene position: 968351-969763 (Clockwise)

Preceding gene: 146338014

Following gene: 146338016

Centisome position: 12.99

GC content: 69.43

Gene sequence:

>1413_bases
ATGAGCACGGAACCCGCTCTGATGACCTTGACGGAGGTTGCCAAGGCGATCGCCGAGAAGAAGCTCTCCTCGGTCGACGT
GACGCGCTCCTGCCTGCATCGCATCGCGCAGTGGCAGCCGAAGCTGAACGCCTTCATGGCGATCGAGTCCGAGCCGGCGC
TGAAGGCCGCCGCCGAGGCCGATGCGGCGCTCGCCAAGGACGGCCCTAAGGGCCCCCTGCATGGCGTGCCGCTGGCGCAT
AAGGACATGTATTACGAGGCCGGCCATGTCTCGACCTGCGGCTCGCTGATCCGCCGCGACTTCGTCGCGACGACCACCTC
GACCGCGCTGCAGCGGCTGAAGGATGCCGGAGCGATCCGGCTCGGCACCTTGCAGATGGCCGAGTTCGCCTATGGTCCGA
CCGGCCACAACGCGCATTACGGTCCGGTGCACAATCCCTGGAAGCTCGGCTACGTCACCGGCGGTTCGTCATCCGGCTCG
GGCTCTGCGGTCGGCGCCCGTCTGACCTTTGCCGCGCTCGGCTCGGACACCGGCGGCTCGATCCGGATGCCCGCGAATTT
CTGCGGCGTCACCGGGCTGAAGGTGACTTGGGGCCGCGTCAGCCGCGCCGGCGCGATGCCGCTGTCGCAGTCGCTCGACA
CGGTCGGCCCGCTGGCGCAGACGGCGGAGGATTGCGCGCTGCTGCTCGGCCTGATGGCCGGTCCGGACCCCGAGGATCCG
ACCGCCAGCGCTGCCCAGGTGCAGGACTATGTCGCGGCCACCCAGTCGTCGATCAAGGGTCTCAAGATCGGCGTGCCCAA
ATCGTTCTACGTCGACGATCTCGATCCCGAGGTGGCGCGCTGCCTCGACGCGGCGATCACGGTGCTGAAGACCGAGGGCG
CCGATGTCGTGCAAGTCGAGCTGCCCGACCAGCGCCAGCTCACCGCGGCGTGCCAGCTCGTGCTGGCGGTCGAGGCGGCG
GCGTTCCACAAGCGCTGGATGATAGAGCGGCCGCAGGATTATGGCCCGCAGGTCCTGATGCGGCTGCAGAACGCGCTGGC
CGTCTCCGGCGTCGCCTATCTCGAGGCGCTGCGCTGGCGCGGCGCCGCGCTGGCGGCGCATGTCGCGGCAACCGCAGGCG
TCGATGCGATCATCGCGCCGGTGTCGCCGCTGGTGACCCCGTCGATCGCCGAGAGCGATGTCGGCGGCGCGCCCGGCGCG
GAGGCCGTGATCCAGCGCATCACCCGCTTTACCCGGCCGGTGAACTATCTCGGCCTGCCGTCGCTGGCGATCCCCACCGG
CTTCACGGCGACGGGGCTCCCGGTTGGCATGCAGCTGATCGGCCGCTCCTTTGACGAAGCCACCATCCTGACCATCGGCG
CCGCCTTCCAACGCGCGACCGATTTCCACGCGCGGGTGCCGACGCTGTCATGA

Upstream 100 bases:

>100_bases
TCGGACCGGCGTTCGACAATTTCGCAGCCATCGCCGGCACGCTGCCGTTCGATCTGGAGCCCGCGAGCTACGTCCTCGCG
CAGACGCTGAAGGTAGGCCG

Downstream 100 bases:

>100_bases
AGCGCTGTGTTGGCGAGGAACTCCGGCCACATGACGCTCAAGGCGATGCAAGAGGTCGGCCTGCGCGCGCCGGAATCGAA
GCTGTCGCCTCACATTCGGT

Product: putative glutamyl-tRNA(Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSTEPALMTLTEVAKAIAEKKLSSVDVTRSCLHRIAQWQPKLNAFMAIESEPALKAAAEADAALAKDGPKGPLHGVPLAH
KDMYYEAGHVSTCGSLIRRDFVATTTSTALQRLKDAGAIRLGTLQMAEFAYGPTGHNAHYGPVHNPWKLGYVTGGSSSGS
GSAVGARLTFAALGSDTGGSIRMPANFCGVTGLKVTWGRVSRAGAMPLSQSLDTVGPLAQTAEDCALLLGLMAGPDPEDP
TASAAQVQDYVAATQSSIKGLKIGVPKSFYVDDLDPEVARCLDAAITVLKTEGADVVQVELPDQRQLTAACQLVLAVEAA
AFHKRWMIERPQDYGPQVLMRLQNALAVSGVAYLEALRWRGAALAAHVAATAGVDAIIAPVSPLVTPSIAESDVGGAPGA
EAVIQRITRFTRPVNYLGLPSLAIPTGFTATGLPVGMQLIGRSFDEATILTIGAAFQRATDFHARVPTLS

Sequences:

>Translated_470_residues
MSTEPALMTLTEVAKAIAEKKLSSVDVTRSCLHRIAQWQPKLNAFMAIESEPALKAAAEADAALAKDGPKGPLHGVPLAH
KDMYYEAGHVSTCGSLIRRDFVATTTSTALQRLKDAGAIRLGTLQMAEFAYGPTGHNAHYGPVHNPWKLGYVTGGSSSGS
GSAVGARLTFAALGSDTGGSIRMPANFCGVTGLKVTWGRVSRAGAMPLSQSLDTVGPLAQTAEDCALLLGLMAGPDPEDP
TASAAQVQDYVAATQSSIKGLKIGVPKSFYVDDLDPEVARCLDAAITVLKTEGADVVQVELPDQRQLTAACQLVLAVEAA
AFHKRWMIERPQDYGPQVLMRLQNALAVSGVAYLEALRWRGAALAAHVAATAGVDAIIAPVSPLVTPSIAESDVGGAPGA
EAVIQRITRFTRPVNYLGLPSLAIPTGFTATGLPVGMQLIGRSFDEATILTIGAAFQRATDFHARVPTLS
>Mature_469_residues
STEPALMTLTEVAKAIAEKKLSSVDVTRSCLHRIAQWQPKLNAFMAIESEPALKAAAEADAALAKDGPKGPLHGVPLAHK
DMYYEAGHVSTCGSLIRRDFVATTTSTALQRLKDAGAIRLGTLQMAEFAYGPTGHNAHYGPVHNPWKLGYVTGGSSSGSG
SAVGARLTFAALGSDTGGSIRMPANFCGVTGLKVTWGRVSRAGAMPLSQSLDTVGPLAQTAEDCALLLGLMAGPDPEDPT
ASAAQVQDYVAATQSSIKGLKIGVPKSFYVDDLDPEVARCLDAAITVLKTEGADVVQVELPDQRQLTAACQLVLAVEAAA
FHKRWMIERPQDYGPQVLMRLQNALAVSGVAYLEALRWRGAALAAHVAATAGVDAIIAPVSPLVTPSIAESDVGGAPGAE
AVIQRITRFTRPVNYLGLPSLAIPTGFTATGLPVGMQLIGRSFDEATILTIGAAFQRATDFHARVPTLS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=31.0276679841897, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI195972892, Length=507, Percent_Identity=28.5996055226824, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI166795287, Length=170, Percent_Identity=32.3529411764706, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=431, Percent_Identity=28.538283062645, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17537465, Length=491, Percent_Identity=26.6802443991853, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17556264, Length=309, Percent_Identity=26.8608414239482, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=307, Percent_Identity=25.7328990228013, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=307, Percent_Identity=25.7328990228013, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=307, Percent_Identity=27.0358306188925, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=273, Percent_Identity=24.1758241758242, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=435, Percent_Identity=25.9770114942529, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=464, Percent_Identity=26.5086206896552, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6319685, Length=402, Percent_Identity=28.6069651741293, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=228, Percent_Identity=29.8245614035088, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648113, Length=501, Percent_Identity=30.5389221556886, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24652985, Length=518, Percent_Identity=27.7992277992278, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI19922090, Length=518, Percent_Identity=27.7992277992278, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24652981, Length=518, Percent_Identity=27.7992277992278, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24652983, Length=518, Percent_Identity=27.7992277992278, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=25.4901960784314, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI161078093, Length=489, Percent_Identity=26.3803680981595, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI21356731, Length=234, Percent_Identity=32.4786324786325, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648435, Length=497, Percent_Identity=23.7424547283702, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24648437, Length=497, Percent_Identity=23.7424547283702, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI45550774, Length=497, Percent_Identity=23.7424547283702, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648441, Length=430, Percent_Identity=23.2558139534884, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648439, Length=430, Percent_Identity=23.2558139534884, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49116; Mature: 48985

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEPALMTLTEVAKAIAEKKLSSVDVTRSCLHRIAQWQPKLNAFMAIESEPALKAAAEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
DAALAKDGPKGPLHGVPLAHKDMYYEAGHVSTCGSLIRRDFVATTTSTALQRLKDAGAIR
HHHHHCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LGTLQMAEFAYGPTGHNAHYGPVHNPWKLGYVTGGSSSGSGSAVGARLTFAALGSDTGGS
ECHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHEEEEEEECCCCCCE
IRMPANFCGVTGLKVTWGRVSRAGAMPLSQSLDTVGPLAQTAEDCALLLGLMAGPDPEDP
EECCHHHHCCCCCEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TASAAQVQDYVAATQSSIKGLKIGVPKSFYVDDLDPEVARCLDAAITVLKTEGADVVQVE
CCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
LPDQRQLTAACQLVLAVEAAAFHKRWMIERPQDYGPQVLMRLQNALAVSGVAYLEALRWR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GAALAAHVAATAGVDAIIAPVSPLVTPSIAESDVGGAPGAEAVIQRITRFTRPVNYLGLP
CHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
SLAIPTGFTATGLPVGMQLIGRSFDEATILTIGAAFQRATDFHARVPTLS
CHHCCCCCCCCCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STEPALMTLTEVAKAIAEKKLSSVDVTRSCLHRIAQWQPKLNAFMAIESEPALKAAAEA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
DAALAKDGPKGPLHGVPLAHKDMYYEAGHVSTCGSLIRRDFVATTTSTALQRLKDAGAIR
HHHHHCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LGTLQMAEFAYGPTGHNAHYGPVHNPWKLGYVTGGSSSGSGSAVGARLTFAALGSDTGGS
ECHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHEEEEEEECCCCCCE
IRMPANFCGVTGLKVTWGRVSRAGAMPLSQSLDTVGPLAQTAEDCALLLGLMAGPDPEDP
EECCHHHHCCCCCEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TASAAQVQDYVAATQSSIKGLKIGVPKSFYVDDLDPEVARCLDAAITVLKTEGADVVQVE
CCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
LPDQRQLTAACQLVLAVEAAAFHKRWMIERPQDYGPQVLMRLQNALAVSGVAYLEALRWR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GAALAAHVAATAGVDAIIAPVSPLVTPSIAESDVGGAPGAEAVIQRITRFTRPVNYLGLP
CHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
SLAIPTGFTATGLPVGMQLIGRSFDEATILTIGAAFQRATDFHARVPTLS
CHHCCCCCCCCCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA