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The map label for this gene is potD [H]

Identifier: 146337981

GI number: 146337981

Start: 925199

End: 926245

Strand: Reverse

Name: potD [H]

Synonym: BRADO0873

Alternate gene names: 146337981

Gene position: 926245-925199 (Counterclockwise)

Preceding gene: 146337982

Following gene: 146337980

Centisome position: 12.42

GC content: 63.61

Gene sequence:

>1047_bases
ATGATGCTTGATCGCCGCCGGCTCCTGACCAGCGCCCTGACGCTGGGGGCCATGCACGCCTTCCCGGGCCTGAGCCTCGC
ACAGGCACGGCCGCTAGTGTTTGCGACCTTCACCGGCAGTTGGGAGGAAGCGCACAAGGCGGTGCTGGTGCCCGCCTTCC
GCAAGGCCAATGGCGATGCGCCCATCGTGCTCGACCCGATGCTGTCGGTCGACCAGATCGCCAAGGTCAACGCCGCCAAG
GCCAATCCGCCGATCGACGTGATGCTGCACGATCCGGGTCCGGCGCTGGTCGCGATCGGGCAGGATCTGGTCGATGCCTA
CCCGGCCGACAAGAGCACCTATTTCAAGGACCTGATTCCAGAGGCGCAGGACGCGATGGGCCCGGCGCCGTTCTTCCAGG
TCGTCGGCCTGACCTACAATCCGGAGACCGTGAAGACGCCACCGACCTCGTGGGCGGATCTGTGGAAGCCCGAGTTCAAG
GGCAAGGTCGGCATCACCAACCTCAACTCCACCTTGGGCACCGGCTGGCTGGTCGAGATTGCGCGCATGCGTGGCGGCTC
CGAAGCCAATGTCGACGAAGGCTTCAAGGCGCTCGAGGAACTGAAGCCGAACCTGGCTGCCGTCGCCGCCAATCCCGGCG
CGCTGGCGACCCTGTTCCAGCAGGGCCAGATCGACATCTCGCCCGGCAATTTCAACGCCATCCAGATCCTCAAAGCCCGC
GGCGTGCCGGTGGAGTTCGTGGCGCCGAAGGAAGGCGCGATCGCGTTCAAGACCACGATCCACATCGTCAAGAACTCGCC
GAACAAGGAGCTCGCCTTCAAGCTGATCGAGGCGGCGCTCTCGCCGGAGGTACAGACGACCCTGATGAACTCGCCCTATC
TGATCGTGCCGACAAACACCAAGGTCGCGATGTCCGGCGAGATCGCCAAGGTGCTGGCAAAGGACACCGCGGAGCTGAAG
AAGAAGTTCGTGTTCCAGGACTGGAAGAAGATCAACGAGCAGCGCGCGTCGTGGATCGAGAAGTTCAACCGCGACATCAA
GATCTGA

Upstream 100 bases:

>100_bases
GCCGTTGCGCGAGGCTGGTAGCGCGGTGCGCATCGCGCCGCTCGCGCCCGGACTCGCCACTGTCTTCCCGCAATGAACAA
CCAACACATCGGAGTGAAAC

Downstream 100 bases:

>100_bases
CGCGATGAAGAGCGCCACGACAGCACGGATGACGGCGCCTGGAGACGTGACCAGCTTCAGCCTGGGGCTTGCACTGCCCT
TGGGCCTGGTGTTCGCGATC

Product: ABC transporter substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MMLDRRRLLTSALTLGAMHAFPGLSLAQARPLVFATFTGSWEEAHKAVLVPAFRKANGDAPIVLDPMLSVDQIAKVNAAK
ANPPIDVMLHDPGPALVAIGQDLVDAYPADKSTYFKDLIPEAQDAMGPAPFFQVVGLTYNPETVKTPPTSWADLWKPEFK
GKVGITNLNSTLGTGWLVEIARMRGGSEANVDEGFKALEELKPNLAAVAANPGALATLFQQGQIDISPGNFNAIQILKAR
GVPVEFVAPKEGAIAFKTTIHIVKNSPNKELAFKLIEAALSPEVQTTLMNSPYLIVPTNTKVAMSGEIAKVLAKDTAELK
KKFVFQDWKKINEQRASWIEKFNRDIKI

Sequences:

>Translated_348_residues
MMLDRRRLLTSALTLGAMHAFPGLSLAQARPLVFATFTGSWEEAHKAVLVPAFRKANGDAPIVLDPMLSVDQIAKVNAAK
ANPPIDVMLHDPGPALVAIGQDLVDAYPADKSTYFKDLIPEAQDAMGPAPFFQVVGLTYNPETVKTPPTSWADLWKPEFK
GKVGITNLNSTLGTGWLVEIARMRGGSEANVDEGFKALEELKPNLAAVAANPGALATLFQQGQIDISPGNFNAIQILKAR
GVPVEFVAPKEGAIAFKTTIHIVKNSPNKELAFKLIEAALSPEVQTTLMNSPYLIVPTNTKVAMSGEIAKVLAKDTAELK
KKFVFQDWKKINEQRASWIEKFNRDIKI
>Mature_348_residues
MMLDRRRLLTSALTLGAMHAFPGLSLAQARPLVFATFTGSWEEAHKAVLVPAFRKANGDAPIVLDPMLSVDQIAKVNAAK
ANPPIDVMLHDPGPALVAIGQDLVDAYPADKSTYFKDLIPEAQDAMGPAPFFQVVGLTYNPETVKTPPTSWADLWKPEFK
GKVGITNLNSTLGTGWLVEIARMRGGSEANVDEGFKALEELKPNLAAVAANPGALATLFQQGQIDISPGNFNAIQILKAR
GVPVEFVAPKEGAIAFKTTIHIVKNSPNKELAFKLIEAALSPEVQTTLMNSPYLIVPTNTKVAMSGEIAKVLAKDTAELK
KKFVFQDWKKINEQRASWIEKFNRDIKI

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=158, Percent_Identity=31.6455696202532, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37809; Mature: 37809

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLDRRRLLTSALTLGAMHAFPGLSLAQARPLVFATFTGSWEEAHKAVLVPAFRKANGDA
CCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEECCCHHHHCCEEEEEHHHCCCCCC
PIVLDPMLSVDQIAKVNAAKANPPIDVMLHDPGPALVAIGQDLVDAYPADKSTYFKDLIP
CEEECCCCCHHHHHHCCCCCCCCCEEEEEECCCCEEEEECHHHHHHCCCCCHHHHHHHCC
EAQDAMGPAPFFQVVGLTYNPETVKTPPTSWADLWKPEFKGKVGITNLNSTLGTGWLVEI
CHHHHCCCCCHHEEEEEEECCCCCCCCCCCHHHCCCCCCCCCEEEEECCCHHCCHHHHHH
ARMRGGSEANVDEGFKALEELKPNLAAVAANPGALATLFQQGQIDISPGNFNAIQILKAR
HHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCEEEECCCCCCEEEEEEEC
GVPVEFVAPKEGAIAFKTTIHIVKNSPNKELAFKLIEAALSPEVQTTLMNSPYLIVPTNT
CCCEEEECCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCC
KVAMSGEIAKVLAKDTAELKKKFVFQDWKKINEQRASWIEKFNRDIKI
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MMLDRRRLLTSALTLGAMHAFPGLSLAQARPLVFATFTGSWEEAHKAVLVPAFRKANGDA
CCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEECCCHHHHCCEEEEEHHHCCCCCC
PIVLDPMLSVDQIAKVNAAKANPPIDVMLHDPGPALVAIGQDLVDAYPADKSTYFKDLIP
CEEECCCCCHHHHHHCCCCCCCCCEEEEEECCCCEEEEECHHHHHHCCCCCHHHHHHHCC
EAQDAMGPAPFFQVVGLTYNPETVKTPPTSWADLWKPEFKGKVGITNLNSTLGTGWLVEI
CHHHHCCCCCHHEEEEEEECCCCCCCCCCCHHHCCCCCCCCCEEEEECCCHHCCHHHHHH
ARMRGGSEANVDEGFKALEELKPNLAAVAANPGALATLFQQGQIDISPGNFNAIQILKAR
HHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCEEEECCCCCCEEEEEEEC
GVPVEFVAPKEGAIAFKTTIHIVKNSPNKELAFKLIEAALSPEVQTTLMNSPYLIVPTNT
CCCEEEECCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCC
KVAMSGEIAKVLAKDTAELKKKFVFQDWKKINEQRASWIEKFNRDIKI
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]