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The map label for this gene is thiE [C]

Identifier: 146337903

GI number: 146337903

Start: 833151

End: 833423

Strand: Direct

Name: thiE [C]

Synonym: BRADO0791

Alternate gene names: 146337903

Gene position: 833151-833423 (Clockwise)

Preceding gene: 146337902

Following gene: 146337907

Centisome position: 11.17

GC content: 69.23

Gene sequence:

>273_bases
GTGCGGCGGTGTCTTGCCAGTTCGTCAAAGGAACAGCAGCAAGCGCCGATCGGCCTCGACGGCCTTGCGCGCATCCTCGC
GGTCTTGCGTCGCCGCGCGCCCAAGCTGCCGGTGTGCGCCAACGCCGGTATCCATGCCGCAAATGCGGCTTCCGTGATCG
CGGCCGGGGCCGATGGGGTAGCTGTCATCTCCGCACTGTCGCGGTCACCCGATCCTCGGGCTGCCGCGCAGCTTCGCCAC
GTCGTCGATCACGAGCTGGCCGCGCGCCGATGA

Upstream 100 bases:

>100_bases
GACACCGCCGCGTGCTCGGTCCCCGTGCAATCATCGGCCTGTCGATCAAATCGACGGCCGAAGCCGAGGCAGCCCCTGTG
GCCCTGATTGATTACGCGGG

Downstream 100 bases:

>100_bases
CCTGCTCCTGAATCGGGACCTGGGAAGCGCACGACCCTGGGGCTCCCTTGGCAGATACTGCCGCGTTGGAGTGCGCATCT
TTGTCAATGAAACAGTCGCC

Product: thiamine-phosphate pyrophosphorylase

Products: Diphosphate; Thiamin monophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MRRCLASSSKEQQQAPIGLDGLARILAVLRRRAPKLPVCANAGIHAANAASVIAAGADGVAVISALSRSPDPRAAAQLRH
VVDHELAARR

Sequences:

>Translated_90_residues
MRRCLASSSKEQQQAPIGLDGLARILAVLRRRAPKLPVCANAGIHAANAASVIAAGADGVAVISALSRSPDPRAAAQLRH
VVDHELAARR
>Mature_90_residues
MRRCLASSSKEQQQAPIGLDGLARILAVLRRRAPKLPVCANAGIHAANAASVIAAGADGVAVISALSRSPDPRAAAQLRH
VVDHELAARR

Specific function: Condenses 4-Methyl-5-(Beta-Hydroxyethyl)-Thiazole Monophosphate (Thz-P) And 4-Amino-5-Hydroxymethyl Pyrimidine Pyrophosphate (Hmp-Pp) To Form Thiamine Monophosphate (TMP). [C]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.5.1.3 [C]

Molecular weight: Translated: 9352; Mature: 9352

Theoretical pI: Translated: 11.89; Mature: 11.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRCLASSSKEQQQAPIGLDGLARILAVLRRRAPKLPVCANAGIHAANAASVIAAGADGV
CCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCHH
AVISALSRSPDPRAAAQLRHVVDHELAARR
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRCLASSSKEQQQAPIGLDGLARILAVLRRRAPKLPVCANAGIHAANAASVIAAGADGV
CCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCHH
AVISALSRSPDPRAAAQLRHVVDHELAARR
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0004 {thiazole} 0.00085 {hydroxymethylpyrimidine} [C]

Substrates: 2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate; 4-Methyl-5-(2-phosphoethyl)-thiazole; H+ [C]

Specific reaction: 2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-Methyl-5-(2-phosphoethyl)-thiazole + H+ --> Diphosphate + Thiamin monophosphate [C]

General reaction: Hydroxymethylpyrimidine group transfer [C]

Inhibitor: Acetyl phosphate substrates; ADP; ATP; CTP; Diphosphate; GTP; Phosphocreatine; Phosphoenolpyruvate; UTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA