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The map label for this gene is proA [H]

Identifier: 146337575

GI number: 146337575

Start: 440646

End: 441935

Strand: Reverse

Name: proA [H]

Synonym: BRADO0430

Alternate gene names: 146337575

Gene position: 441935-440646 (Counterclockwise)

Preceding gene: 146337576

Following gene: 146337574

Centisome position: 5.93

GC content: 68.22

Gene sequence:

>1290_bases
ATGACCGCCCCTCTCAAAGCCATCGACGGCAGTGCTGATCTGCCCCAGCTGATGAACGATCTCGCCAAGCGCGCTCGTGC
GGCGGCGCGGGTGCTGGCGCTGGCGCCGGCCGAGCAGAAGAACCGGGCGCTGGAGGCCATGGAGCGGCGGATCCGCGCCC
GCGCTGACGTGATCATCGCGGCCAATGCCGAGGACGTCGCCGAGGCGAAGGCGGTCGGCGTGACGCCGTCGTTCGTCGAC
CGACTGACGCTGACGCCGGCCCGCGTCGCCGCTATGGCCGACGGGATCGCCGTCGTGCGCGGCGTTGCCGATCCGGTCGG
CGTGGTCACCGAGAGCTGGCAGCGGCCGAACGGCATGACCATCGAGCGCGTGCGGGTGCCGCTCGGCGTCATTGGTGTGA
TCTTCGAGAGCCGTCCCAATGTCGCAGCCGATGCGGGGGTGCTGTGCCTGAAGTCCGGCAACGCCGTGATCCTGCGCGGC
GGCTCGGACTCGTTCCGCTCGTGCCGGGCGATCCATGAATGCCTGGTCGAGGGGCTGCGCGAGGCGGGCTTGCCCGACAC
CGCAATCACGCTGGTGCCGACGCGCGATCGCGCTGCGGTGGGCCTGCTGCTGTCCGGCCTGAATGGCGGCGTCGACGTGA
TTGTGCCGCGCGGGGGCAAGAGCCTCGTCGCGCGCGTCGAGGCCGAGGCGCGCGTGCCGGTATTCGCGCATCTCGAGGGC
GTCAATCACGTCTATGTAGACGGGGCCGCCGATCTCGACATGGCGAAGTCGATCGTGCTGAACGCCAAGATGCGTCGGAC
TGGCGTGTGCGGCGCGGCCGAGACCTTGCTGATCGACCGGGCGTCGCAGGACAAGATCAAGCCACTGGTCGACATGCTGA
TCGGCGCCGGTTGCGAAGTGCGTGGCGACGAGGCGGTGCGCGCGGCCGATGCGCGGGTGACGCCGGCGAACAACGAGGAT
TGGGACACGGAATATCTCGACGCGATCATCGCGGCGAAGGTCGTCGACGGCGTCGACGGCGCGATCGCCCATATCCACGC
GCATGGCTCGCACCACACCGATGCGATCGTAACCCAGGACAACAAAGTGGCTGACAAGTTCCTCAACGAGGTCGACTCGG
CGATCGTCCTGCACAACGCCTCGACGCAGTTCGCCGATGGCGGCGAGTTCGGGTTCGGCGCGGAAATCGGCATCGCCACG
GGCAAGTTTCATGCACGCGGGCCCGTGGGAGTGGAGCAGCTGACGAGCTTCAAATATCGCATCCACGGCACCGGTCAGAC
GCGGCCATGA

Upstream 100 bases:

>100_bases
GGCGGGGGTGGGTCCGGCCTAACGCCGCCTCCGCCGCCAGCCGTCGCAAACTGCGGCTTTCCGTGCTACTGGAGAGCCTT
GCTTCAAGGTTGAAACGCCC

Downstream 100 bases:

>100_bases
CCGCCACGCCCTGTGGTTGAGTTGCAAAAGCCCCCGGCGGTGACCGACGTGACCGATGCCGCCGCCATGCGCCTGGCCAT
CCGCCAGGCCTTGCCTCCCC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTAPLKAIDGSADLPQLMNDLAKRARAAARVLALAPAEQKNRALEAMERRIRARADVIIAANAEDVAEAKAVGVTPSFVD
RLTLTPARVAAMADGIAVVRGVADPVGVVTESWQRPNGMTIERVRVPLGVIGVIFESRPNVAADAGVLCLKSGNAVILRG
GSDSFRSCRAIHECLVEGLREAGLPDTAITLVPTRDRAAVGLLLSGLNGGVDVIVPRGGKSLVARVEAEARVPVFAHLEG
VNHVYVDGAADLDMAKSIVLNAKMRRTGVCGAAETLLIDRASQDKIKPLVDMLIGAGCEVRGDEAVRAADARVTPANNED
WDTEYLDAIIAAKVVDGVDGAIAHIHAHGSHHTDAIVTQDNKVADKFLNEVDSAIVLHNASTQFADGGEFGFGAEIGIAT
GKFHARGPVGVEQLTSFKYRIHGTGQTRP

Sequences:

>Translated_429_residues
MTAPLKAIDGSADLPQLMNDLAKRARAAARVLALAPAEQKNRALEAMERRIRARADVIIAANAEDVAEAKAVGVTPSFVD
RLTLTPARVAAMADGIAVVRGVADPVGVVTESWQRPNGMTIERVRVPLGVIGVIFESRPNVAADAGVLCLKSGNAVILRG
GSDSFRSCRAIHECLVEGLREAGLPDTAITLVPTRDRAAVGLLLSGLNGGVDVIVPRGGKSLVARVEAEARVPVFAHLEG
VNHVYVDGAADLDMAKSIVLNAKMRRTGVCGAAETLLIDRASQDKIKPLVDMLIGAGCEVRGDEAVRAADARVTPANNED
WDTEYLDAIIAAKVVDGVDGAIAHIHAHGSHHTDAIVTQDNKVADKFLNEVDSAIVLHNASTQFADGGEFGFGAEIGIAT
GKFHARGPVGVEQLTSFKYRIHGTGQTRP
>Mature_428_residues
TAPLKAIDGSADLPQLMNDLAKRARAAARVLALAPAEQKNRALEAMERRIRARADVIIAANAEDVAEAKAVGVTPSFVDR
LTLTPARVAAMADGIAVVRGVADPVGVVTESWQRPNGMTIERVRVPLGVIGVIFESRPNVAADAGVLCLKSGNAVILRGG
SDSFRSCRAIHECLVEGLREAGLPDTAITLVPTRDRAAVGLLLSGLNGGVDVIVPRGGKSLVARVEAEARVPVFAHLEGV
NHVYVDGAADLDMAKSIVLNAKMRRTGVCGAAETLLIDRASQDKIKPLVDMLIGAGCEVRGDEAVRAADARVTPANNEDW
DTEYLDAIIAAKVVDGVDGAIAHIHAHGSHHTDAIVTQDNKVADKFLNEVDSAIVLHNASTQFADGGEFGFGAEIGIATG
KFHARGPVGVEQLTSFKYRIHGTGQTRP

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=409, Percent_Identity=36.919315403423, Blast_Score=241, Evalue=7e-64,
Organism=Homo sapiens, GI21361368, Length=409, Percent_Identity=36.919315403423, Blast_Score=241, Evalue=8e-64,
Organism=Escherichia coli, GI1786438, Length=408, Percent_Identity=40.9313725490196, Blast_Score=312, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17569969, Length=414, Percent_Identity=36.9565217391304, Blast_Score=254, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI17569967, Length=414, Percent_Identity=36.9565217391304, Blast_Score=254, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6324899, Length=427, Percent_Identity=37.4707259953162, Blast_Score=265, Evalue=1e-71,
Organism=Drosophila melanogaster, GI21357643, Length=408, Percent_Identity=37.5, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 45095; Mature: 44964

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPLKAIDGSADLPQLMNDLAKRARAAARVLALAPAEQKNRALEAMERRIRARADVIIA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEE
ANAEDVAEAKAVGVTPSFVDRLTLTPARVAAMADGIAVVRGVADPVGVVTESWQRPNGMT
ECCHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCCHHHHHHCCCCHHHHHHCCCCCCCCE
IERVRVPLGVIGVIFESRPNVAADAGVLCLKSGNAVILRGGSDSFRSCRAIHECLVEGLR
EEEEECCCEEEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHH
EAGLPDTAITLVPTRDRAAVGLLLSGLNGGVDVIVPRGGKSLVARVEAEARVPVFAHLEG
HCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCEEEEECC
VNHVYVDGAADLDMAKSIVLNAKMRRTGVCGAAETLLIDRASQDKIKPLVDMLIGAGCEV
CCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHEEECCCCHHHHHHHHHHHCCCCCC
RGDEAVRAADARVTPANNEDWDTEYLDAIIAAKVVDGVDGAIAHIHAHGSHHTDAIVTQD
CCCHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECC
NKVADKFLNEVDSAIVLHNASTQFADGGEFGFGAEIGIATGKFHARGPVGVEQLTSFKYR
CHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCEEEE
IHGTGQTRP
EECCCCCCC
>Mature Secondary Structure 
TAPLKAIDGSADLPQLMNDLAKRARAAARVLALAPAEQKNRALEAMERRIRARADVIIA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEE
ANAEDVAEAKAVGVTPSFVDRLTLTPARVAAMADGIAVVRGVADPVGVVTESWQRPNGMT
ECCHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCCHHHHHHCCCCHHHHHHCCCCCCCCE
IERVRVPLGVIGVIFESRPNVAADAGVLCLKSGNAVILRGGSDSFRSCRAIHECLVEGLR
EEEEECCCEEEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHH
EAGLPDTAITLVPTRDRAAVGLLLSGLNGGVDVIVPRGGKSLVARVEAEARVPVFAHLEG
HCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCEEEEECC
VNHVYVDGAADLDMAKSIVLNAKMRRTGVCGAAETLLIDRASQDKIKPLVDMLIGAGCEV
CCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHEEECCCCHHHHHHHHHHHCCCCCC
RGDEAVRAADARVTPANNEDWDTEYLDAIIAAKVVDGVDGAIAHIHAHGSHHTDAIVTQD
CCCHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECC
NKVADKFLNEVDSAIVLHNASTQFADGGEFGFGAEIGIATGKFHARGPVGVEQLTSFKYR
CHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCEEEE
IHGTGQTRP
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA