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The map label for this gene is pnp [H]

Identifier: 146337231

GI number: 146337231

Start: 65060

End: 67219

Strand: Direct

Name: pnp [H]

Synonym: BRADO0057

Alternate gene names: 146337231

Gene position: 65060-67219 (Clockwise)

Preceding gene: 146337230

Following gene: 146337232

Centisome position: 0.87

GC content: 65.56

Gene sequence:

>2160_bases
ATGTTTACCAAGCATTCCGTCGAGATCGACTGGGGTGGACGTCCCCTCAAACTGGAAACCGGCAAGATCGCGCGCCAGGC
TGACGGCGCCGTCGTCGCCACCTATGGCGAGACCGTCGTGCTGGCGACCGTCGTCGCCGCCAAGGCGCCGCGCGACGGCG
TCGACTTCCTGCCGCTCACCGTCGACTATCAGGAAAAGGCGTACGCTGCGGGGCGCATTCCCGGCGGCTATTTCAAGCGC
GAGGGCCGTCCGACCGAGAAGGAGACGCTGGTCTCCCGCCTGATCGACCGTCCGATCCGCCCGCTTTTCGTCGATTCCTG
GCGCAACGAGACCCAGGTCATCGTGACCGTGCTGTCGCACGACATGGAGAACGATCCGGACATCGTGGCGCTGGTCGCCG
CCTCCGCCGCGCTGACCCTCTCGGGCGTACCGTTCAAGGGCCCGATCGGCGCCGCCCGCGTCGGCTTCGCCAATGACGAA
TACATCCTCAACCCGACCCTCGACGAGATGGCCGACACCCAGCTCGACCTGGTCGTCGCCGGCACCTCGGACGCCGTGCT
GATGGTGGAATCCGAAGCCAAGGAGCTCAACGAGGACATCATGCTCGGCGCGGTGATGTTCGGTCACCGTCACTTCCAGC
CGGTGATCAATGCGATCATCGAGCTCGCCGAAAAGGCCGCCAAGGATCCGCGCGACGTCGCGATCATCGACAACAGCGCG
ATCGAGAAGGAGATGCTGGGCATCGCCGAGCAGGACCTGCGCAAGGCCTACGCGATCGCGATCAAGCAGGAGCGCTACGC
CGCCGTCGGCGCCGTCAAGGAAAAGGTGATGGCGTACTTCTTCCCAGAGGGGCAAGAGCCGAAATACGACAAGCTCCGGG
TCGCCGCCGTGTTCAAGGAGCTCGAGGCCAAAATCGTTCGCTGGAACATCCTGGACACCGGCAAACGCATCGACGGCCGC
GACGCCAAGACGGTGCGCAACATCGTCTGCGAAGTCGGCGTGCTGCCGCGCGCCCATGGCTCGGCGCTGTTCACCCGCGG
TGAGACCCAGGCGCTGGTGGTGACCACGCTCGGCACCGGCGAGGACGAGCAGTACATCGACGCGCTGTCGGGAACGTACA
AAGAGACGTTCCTGCTGCATTACAACTTCCCGCCCTATTCGGTCGGTGAGACCGGCCGCCTTGGCGGCACCAAGCGCCGC
GAGATCGGCCATGGCAAGCTGGCGTGGCGCGCGATCCACCCGGTGCTGCCGCCGCACCACGAGTTCCCCTACACCACCCG
GGTGGTGTCGGAAGTGACGGAGTCCAACGGCTCGTCGTCGATGGCGACCGTCTGCGGCTCGTCGCTGGCGCTGATGGATG
CCGGCGTCCCCTTGAAGCGGCCGACCGCGGGTATCGCGATGGGCCTGATCCTGGAAGGTAGCCGCTTCGCCGTGCTGTCC
GACATTCTCGGCGACGAGGACCATCTCGGCGACATGGACTTCAAGGTGGCGGGCACTGAGTCCGGCATCACCTCGCTGCA
GATGGACATCAAGATCGAGGGCATCACCGAGGAGATCATGCGCGTGGCGCTCGGCCAGGCGCGCGAGGGCCGCATCCACA
TCCTGGGCGAAATGTCCAAGGCGCTGACCGCAGCCCGCGCCGAGCTTGGCGAATACGCGCCGCGTATCGAGACCTTCAAG
ATCGCCACCGACAAGATCCGCGAAGTGATTGGCACCGGCGGCAAGGTGATCCGCGAGATCGTCGAGAAGACCGGCGCCAA
GGTCAACATCGAGGACGACGGCACCGTGAAGGTCGCCTCCAGCGACGGCGAGGCCATGAAGGCCGCCATCAAGTGGATCA
AGTCGATCGCCTCCGATCCGGAGGTCGGCCAGATCTATGACGGCACCGTCGTCAAGGTGATGGAGTTCGGCGCCTTCGTG
AACTTCTTCGGCACCCGCGACGGCCTCGTGCACATCTCGCAGCTCGCCGACAAGCGCGTGCAGAAGACCACCGACGTCGT
CAAGGAAGGCGACAAGGTCAAGGTCAAGCTGCTCGGCTTCGACGACCGCGGCAAGACCCGGCTGTCGATGAAGGTCGTCG
ACCAGACCACCGGCGAGGACCTCGAGGCCAAGCAAAAGGACGCCCCCGCCGAGGCGCCGCGCGAGGCCGCCGGCGAGTAA

Upstream 100 bases:

>100_bases
GATCGCAGGGCGCTGATCGCAATCCAGCGCCCTGCAATCTTGCGCATGGTTTTTTTGTTTTGGGGGTCTGCCTTCGCTCA
TCCTATGAAAGAAGAGACCC

Downstream 100 bases:

>100_bases
GCGGCGCAGGAGTTTCGAGAGACACCAAAGGGCGGCTTTCGAGCCGCCCTTTTTCATTATCGGTCGCAGGTCCCTCCAGG
TCATTGCGAGCGAAGCGGCG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 719; Mature: 719

Protein sequence:

>719_residues
MFTKHSVEIDWGGRPLKLETGKIARQADGAVVATYGETVVLATVVAAKAPRDGVDFLPLTVDYQEKAYAAGRIPGGYFKR
EGRPTEKETLVSRLIDRPIRPLFVDSWRNETQVIVTVLSHDMENDPDIVALVAASAALTLSGVPFKGPIGAARVGFANDE
YILNPTLDEMADTQLDLVVAGTSDAVLMVESEAKELNEDIMLGAVMFGHRHFQPVINAIIELAEKAAKDPRDVAIIDNSA
IEKEMLGIAEQDLRKAYAIAIKQERYAAVGAVKEKVMAYFFPEGQEPKYDKLRVAAVFKELEAKIVRWNILDTGKRIDGR
DAKTVRNIVCEVGVLPRAHGSALFTRGETQALVVTTLGTGEDEQYIDALSGTYKETFLLHYNFPPYSVGETGRLGGTKRR
EIGHGKLAWRAIHPVLPPHHEFPYTTRVVSEVTESNGSSSMATVCGSSLALMDAGVPLKRPTAGIAMGLILEGSRFAVLS
DILGDEDHLGDMDFKVAGTESGITSLQMDIKIEGITEEIMRVALGQAREGRIHILGEMSKALTAARAELGEYAPRIETFK
IATDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVASSDGEAMKAAIKWIKSIASDPEVGQIYDGTVVKVMEFGAFV
NFFGTRDGLVHISQLADKRVQKTTDVVKEGDKVKVKLLGFDDRGKTRLSMKVVDQTTGEDLEAKQKDAPAEAPREAAGE

Sequences:

>Translated_719_residues
MFTKHSVEIDWGGRPLKLETGKIARQADGAVVATYGETVVLATVVAAKAPRDGVDFLPLTVDYQEKAYAAGRIPGGYFKR
EGRPTEKETLVSRLIDRPIRPLFVDSWRNETQVIVTVLSHDMENDPDIVALVAASAALTLSGVPFKGPIGAARVGFANDE
YILNPTLDEMADTQLDLVVAGTSDAVLMVESEAKELNEDIMLGAVMFGHRHFQPVINAIIELAEKAAKDPRDVAIIDNSA
IEKEMLGIAEQDLRKAYAIAIKQERYAAVGAVKEKVMAYFFPEGQEPKYDKLRVAAVFKELEAKIVRWNILDTGKRIDGR
DAKTVRNIVCEVGVLPRAHGSALFTRGETQALVVTTLGTGEDEQYIDALSGTYKETFLLHYNFPPYSVGETGRLGGTKRR
EIGHGKLAWRAIHPVLPPHHEFPYTTRVVSEVTESNGSSSMATVCGSSLALMDAGVPLKRPTAGIAMGLILEGSRFAVLS
DILGDEDHLGDMDFKVAGTESGITSLQMDIKIEGITEEIMRVALGQAREGRIHILGEMSKALTAARAELGEYAPRIETFK
IATDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVASSDGEAMKAAIKWIKSIASDPEVGQIYDGTVVKVMEFGAFV
NFFGTRDGLVHISQLADKRVQKTTDVVKEGDKVKVKLLGFDDRGKTRLSMKVVDQTTGEDLEAKQKDAPAEAPREAAGE
>Mature_719_residues
MFTKHSVEIDWGGRPLKLETGKIARQADGAVVATYGETVVLATVVAAKAPRDGVDFLPLTVDYQEKAYAAGRIPGGYFKR
EGRPTEKETLVSRLIDRPIRPLFVDSWRNETQVIVTVLSHDMENDPDIVALVAASAALTLSGVPFKGPIGAARVGFANDE
YILNPTLDEMADTQLDLVVAGTSDAVLMVESEAKELNEDIMLGAVMFGHRHFQPVINAIIELAEKAAKDPRDVAIIDNSA
IEKEMLGIAEQDLRKAYAIAIKQERYAAVGAVKEKVMAYFFPEGQEPKYDKLRVAAVFKELEAKIVRWNILDTGKRIDGR
DAKTVRNIVCEVGVLPRAHGSALFTRGETQALVVTTLGTGEDEQYIDALSGTYKETFLLHYNFPPYSVGETGRLGGTKRR
EIGHGKLAWRAIHPVLPPHHEFPYTTRVVSEVTESNGSSSMATVCGSSLALMDAGVPLKRPTAGIAMGLILEGSRFAVLS
DILGDEDHLGDMDFKVAGTESGITSLQMDIKIEGITEEIMRVALGQAREGRIHILGEMSKALTAARAELGEYAPRIETFK
IATDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVASSDGEAMKAAIKWIKSIASDPEVGQIYDGTVVKVMEFGAFV
NFFGTRDGLVHISQLADKRVQKTTDVVKEGDKVKVKLLGFDDRGKTRLSMKVVDQTTGEDLEAKQKDAPAEAPREAAGE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=720, Percent_Identity=37.9166666666667, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI4826690, Length=92, Percent_Identity=50, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI21361576, Length=87, Percent_Identity=45.9770114942529, Blast_Score=70, Evalue=8e-12,
Organism=Escherichia coli, GI145693187, Length=689, Percent_Identity=57.0391872278665, Blast_Score=780, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=105, Percent_Identity=41.9047619047619, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115534063, Length=715, Percent_Identity=33.5664335664336, Blast_Score=356, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI17535281, Length=95, Percent_Identity=48.4210526315789, Blast_Score=87, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320850, Length=94, Percent_Identity=40.4255319148936, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281362905, Length=718, Percent_Identity=38.0222841225627, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651641, Length=718, Percent_Identity=38.0222841225627, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651643, Length=718, Percent_Identity=38.0222841225627, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI161079377, Length=655, Percent_Identity=37.5572519083969, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI20129977, Length=91, Percent_Identity=47.2527472527472, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 78162; Mature: 78162

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTKHSVEIDWGGRPLKLETGKIARQADGAVVATYGETVVLATVVAAKAPRDGVDFLPLT
CCCCEEEEEECCCEEEEEECCCHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCEEEEE
VDYQEKAYAAGRIPGGYFKREGRPTEKETLVSRLIDRPIRPLFVDSWRNETQVIVTVLSH
ECCCHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEEECC
DMENDPDIVALVAASAALTLSGVPFKGPIGAARVGFANDEYILNPTLDEMADTQLDLVVA
CCCCCCCEEEEEEHHHHEEECCCCCCCCCCCCEECCCCCCEEECCCHHHHCCCCEEEEEE
GTSDAVLMVESEAKELNEDIMLGAVMFGHRHFQPVINAIIELAEKAAKDPRDVAIIDNSA
CCCCEEEEEECHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHH
IEKEMLGIAEQDLRKAYAIAIKQERYAAVGAVKEKVMAYFFPEGQEPKYDKLRVAAVFKE
HHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHH
LEAKIVRWNILDTGKRIDGRDAKTVRNIVCEVGVLPRAHGSALFTRGETQALVVTTLGTG
HHHEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECCC
EDEQYIDALSGTYKETFLLHYNFPPYSVGETGRLGGTKRREIGHGKLAWRAIHPVLPPHH
CCHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
EFPYTTRVVSEVTESNGSSSMATVCGSSLALMDAGVPLKRPTAGIAMGLILEGSRFAVLS
CCCHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCCCCCCCCEEEEEEECCCCHHHHH
DILGDEDHLGDMDFKVAGTESGITSLQMDIKIEGITEEIMRVALGQAREGRIHILGEMSK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHCCCCCCCEEEEEHHHH
ALTAARAELGEYAPRIETFKIATDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVAS
HHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEC
SDGEAMKAAIKWIKSIASDPEVGQIYDGTVVKVMEFGAFVNFFGTRDGLVHISQLADKRV
CCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
QKTTDVVKEGDKVKVKLLGFDDRGKTRLSMKVVDQTTGEDLEAKQKDAPAEAPREAAGE
HHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCCHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure
MFTKHSVEIDWGGRPLKLETGKIARQADGAVVATYGETVVLATVVAAKAPRDGVDFLPLT
CCCCEEEEEECCCEEEEEECCCHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCEEEEE
VDYQEKAYAAGRIPGGYFKREGRPTEKETLVSRLIDRPIRPLFVDSWRNETQVIVTVLSH
ECCCHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEEECC
DMENDPDIVALVAASAALTLSGVPFKGPIGAARVGFANDEYILNPTLDEMADTQLDLVVA
CCCCCCCEEEEEEHHHHEEECCCCCCCCCCCCEECCCCCCEEECCCHHHHCCCCEEEEEE
GTSDAVLMVESEAKELNEDIMLGAVMFGHRHFQPVINAIIELAEKAAKDPRDVAIIDNSA
CCCCEEEEEECHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHH
IEKEMLGIAEQDLRKAYAIAIKQERYAAVGAVKEKVMAYFFPEGQEPKYDKLRVAAVFKE
HHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHH
LEAKIVRWNILDTGKRIDGRDAKTVRNIVCEVGVLPRAHGSALFTRGETQALVVTTLGTG
HHHEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECCC
EDEQYIDALSGTYKETFLLHYNFPPYSVGETGRLGGTKRREIGHGKLAWRAIHPVLPPHH
CCHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
EFPYTTRVVSEVTESNGSSSMATVCGSSLALMDAGVPLKRPTAGIAMGLILEGSRFAVLS
CCCHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCCCCCCCCEEEEEEECCCCHHHHH
DILGDEDHLGDMDFKVAGTESGITSLQMDIKIEGITEEIMRVALGQAREGRIHILGEMSK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHCCCCCCCEEEEEHHHH
ALTAARAELGEYAPRIETFKIATDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVAS
HHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEC
SDGEAMKAAIKWIKSIASDPEVGQIYDGTVVKVMEFGAFVNFFGTRDGLVHISQLADKRV
CCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
QKTTDVVKEGDKVKVKLLGFDDRGKTRLSMKVVDQTTGEDLEAKQKDAPAEAPREAAGE
HHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCCHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA