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The map label for this gene is truB [H]

Identifier: 146337229

GI number: 146337229

Start: 63334

End: 64431

Strand: Direct

Name: truB [H]

Synonym: BRADO0055

Alternate gene names: 146337229

Gene position: 63334-64431 (Clockwise)

Preceding gene: 146337228

Following gene: 146337230

Centisome position: 0.85

GC content: 65.94

Gene sequence:

>1098_bases
ATGACCATTCCCCCGGAAACCGGCGCTGTCGATGCGCTCAGCATCGTGCCCAAGGCCGTCAACGTTCAAAAAAATTCTTC
CGACGCGCGTCCTCCGCGCGACAACAACGATCCGCGCAACAAGCAGCAGAGGGGCAATCAGCCGCGCCGCGATCGTCGCG
ACGTTCATGGCTGGGTCGTGCTCGACAAGCCGATCGGCATGACCTCGACGCAGGCCGTCGCGGTCGCCAAGCGCCTGTTC
CAGGCCAAGCGCGCCGGCCATGCCGGCACGCTCGATCCGCTCGCCTCCGGCGGGCTTCCGATCGCGCTCGGCGAGGCCAC
CAAGACCGTTCCGTTCGTGATGGACGGCCGCAAGCGCTACCGTTTCACCGTCACCTGGGGTGCGGAGCGCGATACCGATG
ACACCGAGGGGCGGGTGGTCGAGACCAGCGAGAACCGGCCGGATGTCGCCGCGATCCGCGCACTGCTTCCGCAATTTACC
GGCCGGATCGAGCAGACTCCGCCGCGTTATTCCGCGATCAAGATCCAGGGCGAGCGCGCCTATGATCTGGCCCGCGACGG
CGAGGTGGTCGAGCTGCAGCCGCGCCCGGTCGAGATTCATGAACTGACCCTCGTCGACCAGCCCGACGCCGACCATTCGG
TGTTCGAGGCGGAATGTGGCAAGGGAACCTATGTCAGGGCCCTGGCGCGCGACATGGGTCGGCTCCTCGGCTGCTACGGC
CATATCAGCGCCCTCCGGCGGACTTTGGTCGGTCCGTTTACCGAGAACGACATGATTCCGCTGGAACAGCTGGAGGCTTT
GTGCAATAGAGCCGCGTCCGGTGAGGGAAGCCTCGCCGACGCACTGCTGCCCGTTGAGACCGCGCTGGACGACATCCCGG
CACTGGCCGTCACGCGGGCGGATGCGGCAAGGCTCCATCGGGGCCAGGCCGTCTTGTTGCGCGGACGGGATGCGCCCCAG
AGTAGCGGCACAGTCTATGTCACGGTCGCAGGCCGCCTTCTGGCGCTTGCCGAAATTGGCAATGGCGAACTCATCCCCAA
GCGCGTGTTCAACCTGACCGGACTGACTGCCAGCTCCGGCCGCAACGAGAGAGATTGA

Upstream 100 bases:

>100_bases
TCCGCGTCGACGAGCGATTCGACGAAGCGGAACGGATCGAGAAATTACTCAGAACACCCGCGGTGCAGAGAGATCTCAAT
TCGGATTTGGAAGAGAGCTG

Downstream 100 bases:

>100_bases
CGATGTCGATTACCGCAGAGCGCAAGGCGGAAATCATCAAGGCGAACGCCACCAAGGCCGGCGACACCGGCTCCCCCGAA
GTGCAGGTTGCGATCCTGTC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTIPPETGAVDALSIVPKAVNVQKNSSDARPPRDNNDPRNKQQRGNQPRRDRRDVHGWVVLDKPIGMTSTQAVAVAKRLF
QAKRAGHAGTLDPLASGGLPIALGEATKTVPFVMDGRKRYRFTVTWGAERDTDDTEGRVVETSENRPDVAAIRALLPQFT
GRIEQTPPRYSAIKIQGERAYDLARDGEVVELQPRPVEIHELTLVDQPDADHSVFEAECGKGTYVRALARDMGRLLGCYG
HISALRRTLVGPFTENDMIPLEQLEALCNRAASGEGSLADALLPVETALDDIPALAVTRADAARLHRGQAVLLRGRDAPQ
SSGTVYVTVAGRLLALAEIGNGELIPKRVFNLTGLTASSGRNERD

Sequences:

>Translated_365_residues
MTIPPETGAVDALSIVPKAVNVQKNSSDARPPRDNNDPRNKQQRGNQPRRDRRDVHGWVVLDKPIGMTSTQAVAVAKRLF
QAKRAGHAGTLDPLASGGLPIALGEATKTVPFVMDGRKRYRFTVTWGAERDTDDTEGRVVETSENRPDVAAIRALLPQFT
GRIEQTPPRYSAIKIQGERAYDLARDGEVVELQPRPVEIHELTLVDQPDADHSVFEAECGKGTYVRALARDMGRLLGCYG
HISALRRTLVGPFTENDMIPLEQLEALCNRAASGEGSLADALLPVETALDDIPALAVTRADAARLHRGQAVLLRGRDAPQ
SSGTVYVTVAGRLLALAEIGNGELIPKRVFNLTGLTASSGRNERD
>Mature_364_residues
TIPPETGAVDALSIVPKAVNVQKNSSDARPPRDNNDPRNKQQRGNQPRRDRRDVHGWVVLDKPIGMTSTQAVAVAKRLFQ
AKRAGHAGTLDPLASGGLPIALGEATKTVPFVMDGRKRYRFTVTWGAERDTDDTEGRVVETSENRPDVAAIRALLPQFTG
RIEQTPPRYSAIKIQGERAYDLARDGEVVELQPRPVEIHELTLVDQPDADHSVFEAECGKGTYVRALARDMGRLLGCYGH
ISALRRTLVGPFTENDMIPLEQLEALCNRAASGEGSLADALLPVETALDDIPALAVTRADAARLHRGQAVLLRGRDAPQS
SGTVYVTVAGRLLALAEIGNGELIPKRVFNLTGLTASSGRNERD

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=216, Percent_Identity=37.5, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI2367200, Length=311, Percent_Identity=37.620578778135, Blast_Score=194, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI17553978, Length=201, Percent_Identity=28.3582089552239, Blast_Score=81, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=253, Percent_Identity=30.8300395256917, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=134, Percent_Identity=33.5820895522388, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI281364189, Length=204, Percent_Identity=28.4313725490196, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281364187, Length=204, Percent_Identity=28.4313725490196, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281364185, Length=204, Percent_Identity=28.4313725490196, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281364183, Length=204, Percent_Identity=28.4313725490196, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI62471759, Length=204, Percent_Identity=28.4313725490196, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17975520, Length=204, Percent_Identity=28.4313725490196, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 39610; Mature: 39479

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPPETGAVDALSIVPKAVNVQKNSSDARPPRDNNDPRNKQQRGNQPRRDRRDVHGWVV
CCCCCCCCCCHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHCCCCEEEE
LDKPIGMTSTQAVAVAKRLFQAKRAGHAGTLDPLASGGLPIALGEATKTVPFVMDGRKRY
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEECCCCCCCCEEECCCEEE
RFTVTWGAERDTDDTEGRVVETSENRPDVAAIRALLPQFTGRIEQTPPRYSAIKIQGERA
EEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
YDLARDGEVVELQPRPVEIHELTLVDQPDADHSVFEAECGKGTYVRALARDMGRLLGCYG
CHHCCCCCEEEECCCCEEEEEEEEECCCCCCCCHHECCCCCCHHHHHHHHHHHHHHHHHH
HISALRRTLVGPFTENDMIPLEQLEALCNRAASGEGSLADALLPVETALDDIPALAVTRA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHEEEHH
DAARLHRGQAVLLRGRDAPQSSGTVYVTVAGRLLALAEIGNGELIPKRVFNLTGLTASSG
HHHHHCCCCEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCCCCHHHHHHHCCCCCCCC
RNERD
CCCCC
>Mature Secondary Structure 
TIPPETGAVDALSIVPKAVNVQKNSSDARPPRDNNDPRNKQQRGNQPRRDRRDVHGWVV
CCCCCCCCCHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHCCCCEEEE
LDKPIGMTSTQAVAVAKRLFQAKRAGHAGTLDPLASGGLPIALGEATKTVPFVMDGRKRY
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEECCCCCCCCEEECCCEEE
RFTVTWGAERDTDDTEGRVVETSENRPDVAAIRALLPQFTGRIEQTPPRYSAIKIQGERA
EEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
YDLARDGEVVELQPRPVEIHELTLVDQPDADHSVFEAECGKGTYVRALARDMGRLLGCYG
CHHCCCCCEEEECCCCEEEEEEEEECCCCCCCCHHECCCCCCHHHHHHHHHHHHHHHHHH
HISALRRTLVGPFTENDMIPLEQLEALCNRAASGEGSLADALLPVETALDDIPALAVTRA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHEEEHH
DAARLHRGQAVLLRGRDAPQSSGTVYVTVAGRLLALAEIGNGELIPKRVFNLTGLTASSG
HHHHHCCCCEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCCCCHHHHHHHCCCCCCCC
RNERD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]