Click here to switch to the map view.

The map label for this gene is trmB [H]

Identifier: 146337222

GI number: 146337222

Start: 54463

End: 55194

Strand: Direct

Name: trmB [H]

Synonym: BRADO0048

Alternate gene names: 146337222

Gene position: 54463-55194 (Clockwise)

Preceding gene: 146337221

Following gene: 146337224

Centisome position: 0.73

GC content: 68.31

Gene sequence:

>732_bases
ATGAGCCTTGCGATGATGCCCATCGACGACGATCACACCGGCGACGGAGCCCTGCATGGCCAGGGCTCGTTCTTCGGCCG
GCGCAAGGGTCACAAGCTGCGGACGCACCAGGCCGACCTGGTCGAGAATCTGCTGCCGCATCTCGCGCTCGACCTGTCGG
CCCCCGCCGACGTCGTGCTGCCGACGCTGTATGATCCACCGGTCGACGCGCTGCGGCTCGAGATCGGCTTCGGCGGCGGC
GAGCATCTCGCGGCGGAGGCCAAGGCCTACCCCACGACGGGCTTCATCGGCTGCGAGCCCTATGTCAACGGCATGGCCAA
GATCCTCTCGGCGATCGAGGCCGACGACATCGGCAACATCCGGCTGCTCGCTGGTGACGCAGCCGAGCTGCTGGCCTGGC
TGCCCCCGCAGTCGCTGCAGCGGATCGATCTCATTCATCCGGATCCATGGCCGAAGCGGCGGCATTGGAAGCGGCGCTTC
GTGCAGGGTGCTACGATTGCCGCGATGGCCCGCGTGCTGAAGCCAGGCGGCGAGTTCCGCTTCGTCAGCGACATCGCCGA
CTATTGCGCCTGGACGCTGTGGCACATGCTGCGCGCGCCGGATTTCGTCTGGACCGCGGAGCGCGCCGACGACTGGCGCC
TGCCGTGGCCGGGCTACACGATGACCCGCTACGGCCGCAAGGCGACGCGCGAGGGTCGCGTCGCCAACTACCTGATCTTC
AGCCGGCGCTGA

Upstream 100 bases:

>100_bases
GCGTCGACGGCAAGGGCGCGCATACGCATTACGAACAGATGTACATCTCCTCGATCGAGCCGCGGGCGCGTCTGCTCCAC
CGGCTATATCAGACGCTGCG

Downstream 100 bases:

>100_bases
AGCGCCGTGGCGGGCGGTCAGTGCCGCGTCTTCCAGAACGCCACGACGCGCTCGGCCGTCGACGACAGCAGGCGCGCATA
GTTGACGCGCAGCGCGTCGA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSLAMMPIDDDHTGDGALHGQGSFFGRRKGHKLRTHQADLVENLLPHLALDLSAPADVVLPTLYDPPVDALRLEIGFGGG
EHLAAEAKAYPTTGFIGCEPYVNGMAKILSAIEADDIGNIRLLAGDAAELLAWLPPQSLQRIDLIHPDPWPKRRHWKRRF
VQGATIAAMARVLKPGGEFRFVSDIADYCAWTLWHMLRAPDFVWTAERADDWRLPWPGYTMTRYGRKATREGRVANYLIF
SRR

Sequences:

>Translated_243_residues
MSLAMMPIDDDHTGDGALHGQGSFFGRRKGHKLRTHQADLVENLLPHLALDLSAPADVVLPTLYDPPVDALRLEIGFGGG
EHLAAEAKAYPTTGFIGCEPYVNGMAKILSAIEADDIGNIRLLAGDAAELLAWLPPQSLQRIDLIHPDPWPKRRHWKRRF
VQGATIAAMARVLKPGGEFRFVSDIADYCAWTLWHMLRAPDFVWTAERADDWRLPWPGYTMTRYGRKATREGRVANYLIF
SRR
>Mature_242_residues
SLAMMPIDDDHTGDGALHGQGSFFGRRKGHKLRTHQADLVENLLPHLALDLSAPADVVLPTLYDPPVDALRLEIGFGGGE
HLAAEAKAYPTTGFIGCEPYVNGMAKILSAIEADDIGNIRLLAGDAAELLAWLPPQSLQRIDLIHPDPWPKRRHWKRRFV
QGATIAAMARVLKPGGEFRFVSDIADYCAWTLWHMLRAPDFVWTAERADDWRLPWPGYTMTRYGRKATREGRVANYLIFS
RR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=222, Percent_Identity=31.5315315315315, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 27251; Mature: 27119

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAMMPIDDDHTGDGALHGQGSFFGRRKGHKLRTHQADLVENLLPHLALDLSAPADVVL
CCEEEECCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCEEE
PTLYDPPVDALRLEIGFGGGEHLAAEAKAYPTTGFIGCEPYVNGMAKILSAIEADDIGNI
CCCCCCCCCCEEEEEECCCCCHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCE
RLLAGDAAELLAWLPPQSLQRIDLIHPDPWPKRRHWKRRFVQGATIAAMARVLKPGGEFR
EEEECCHHHHHHHCCCHHHCEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCEE
FVSDIADYCAWTLWHMLRAPDFVWTAERADDWRLPWPGYTMTRYGRKATREGRVANYLIF
EHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHCCHHHCCCCEEEEEEE
SRR
ECC
>Mature Secondary Structure 
SLAMMPIDDDHTGDGALHGQGSFFGRRKGHKLRTHQADLVENLLPHLALDLSAPADVVL
CEEEECCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCEEE
PTLYDPPVDALRLEIGFGGGEHLAAEAKAYPTTGFIGCEPYVNGMAKILSAIEADDIGNI
CCCCCCCCCCEEEEEECCCCCHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCE
RLLAGDAAELLAWLPPQSLQRIDLIHPDPWPKRRHWKRRFVQGATIAAMARVLKPGGEFR
EEEECCHHHHHHHCCCHHHCEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCEE
FVSDIADYCAWTLWHMLRAPDFVWTAERADDWRLPWPGYTMTRYGRKATREGRVANYLIF
EHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHCCHHHCCCCEEEEEEE
SRR
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA