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The map label for this gene is norM [H]

Identifier: 146337184

GI number: 146337184

Start: 12876

End: 14252

Strand: Direct

Name: norM [H]

Synonym: BRADO0009

Alternate gene names: 146337184

Gene position: 12876-14252 (Clockwise)

Preceding gene: 146337181

Following gene: 146337186

Centisome position: 0.17

GC content: 67.25

Gene sequence:

>1377_bases
ATGACCTCCTCAACGGCAACCGGCCACGCGCCGCCAACACCACCCTCTCTCGCGCTCGAACTCTGGGACACCATCCGCCT
CGCCGCCCCGATGGCGCTGACGCAGCTGTCGCAGATCGCGATGATGTCGACCGATCTCGCCTTCATCGGCCGTCTGGGCG
GCGACGCCGTCGCAGCGGCCGCCTTGGCCGGCACCGTGTATTTCGTCGGCTCGACCTTCGGCATGGGCTTGATGTCGGCG
GTCGCGCCGCTGGCCGCGCAGGCGTTCGGCGCTGATGATGCCGGACTGGTGCGGCGCGCGCTACGCGTCGGGCTGTGGGC
CGGCCTGTTCGTCGCGCTGCCGATCATGCTGATCGCGCTGAATGGCGAGACTATCCTGCTCGCGCTCTGCCAAGCCCCAG
GCGTCGCGCATCTCGCGCAACATTATCTGTCCGGCCTCGCCTGGGGCGTCGCGCCGATGCTGTGGTTCCTGGCGCTGCGC
AGCTTCATGGGCGCCGTGAATCGTCCCGAGCCGGTGCTGTGGATCACGCTGGCCGCGATCCCGCTCAATGCGCTGCTGGT
CTATCCACTGCTCTACGGCCATTGGGGCCTTCCGCAACTCGGCCTGACCGGCGTCGGGCTCGCGACATCGAGCGTCAATC
TGGGCACGTTTCTTGCCGGGCTGTGGTTCGCAGCATTCCGCCCACCGTTCCGCAACTATCATGTCCTGGCGCAGCTCGGC
CGCATCGACTGGCGGCTGCTGCGGCAGCTGCTCGTCATCGGTGCGCCGATCTCGCTGTCGTTCCTGCTCGAATATGGACT
GTTCTCGGCCGCCGCGATCCTGATGGGACTGATCAGCACCACTGCGCTCGCCGCGCATCAGATCGCGCTGCAAATCACTG
CGATCCTGTTCATGGTGCCGTTCGGCATCAGCATGGCCGCGACGGTGCGCGTCGGACATGCTGCAGGACGTCAGGATCCG
CACGCTGTCCGTCGTGCCGGCTTGATGGCGATGCTGCTCGGGCTCGGGCTCGCGGTGCTGTTCACGCTCGGCATCATCGC
CGCGCGGGTCGAGATCGTGCAGCTGTTTCTCGGGAGCGCGGTGGCCGATGCCGAGGCGACCGTCGCGCTGGCGGCCACGC
TGCTGCTGGTCGGCACCACCTTCTTCATCACCGACGCGCTGCAGACGATCACGGTTGGCGCGCTGCGCGGCATCAAGGAT
ACGCGTATCCCGCTGCTGCTCGCGGTGCTCGGCTATTGGCTGATCGGCTTTCCCTTGTCCTACGTGCTCGGCCTTCACAC
CGCCTTCGGCGCGATCGGGATCTGGATCGGGCTGTCGATCGGCACCGGCCTCTACGCGGCGCTGCTGGTGCTGCGCTTTC
TCAGGCTGTCGCGATGA

Upstream 100 bases:

>100_bases
CATCTGCTTCGTCGGTCTGCGCTCCTACGCCGCACATCCCCGCGACGGAACCTGCCCGCATGATGTGCATGCCATCACGG
GGACTGCTATAGCCGCGCGC

Downstream 100 bases:

>100_bases
GCTCAGGCCCCGGCCGTGTCCGACGTCTCCTCCGGCTCCGGCCGATGCGGCTTGCCGCTCGAGGCGCCGGCCCATTCCGC
GACCACACGCTCGAGATCGA

Product: putative MATE efflux family protein multidrug resistance protein norM

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTSSTATGHAPPTPPSLALELWDTIRLAAPMALTQLSQIAMMSTDLAFIGRLGGDAVAAAALAGTVYFVGSTFGMGLMSA
VAPLAAQAFGADDAGLVRRALRVGLWAGLFVALPIMLIALNGETILLALCQAPGVAHLAQHYLSGLAWGVAPMLWFLALR
SFMGAVNRPEPVLWITLAAIPLNALLVYPLLYGHWGLPQLGLTGVGLATSSVNLGTFLAGLWFAAFRPPFRNYHVLAQLG
RIDWRLLRQLLVIGAPISLSFLLEYGLFSAAAILMGLISTTALAAHQIALQITAILFMVPFGISMAATVRVGHAAGRQDP
HAVRRAGLMAMLLGLGLAVLFTLGIIAARVEIVQLFLGSAVADAEATVALAATLLLVGTTFFITDALQTITVGALRGIKD
TRIPLLLAVLGYWLIGFPLSYVLGLHTAFGAIGIWIGLSIGTGLYAALLVLRFLRLSR

Sequences:

>Translated_458_residues
MTSSTATGHAPPTPPSLALELWDTIRLAAPMALTQLSQIAMMSTDLAFIGRLGGDAVAAAALAGTVYFVGSTFGMGLMSA
VAPLAAQAFGADDAGLVRRALRVGLWAGLFVALPIMLIALNGETILLALCQAPGVAHLAQHYLSGLAWGVAPMLWFLALR
SFMGAVNRPEPVLWITLAAIPLNALLVYPLLYGHWGLPQLGLTGVGLATSSVNLGTFLAGLWFAAFRPPFRNYHVLAQLG
RIDWRLLRQLLVIGAPISLSFLLEYGLFSAAAILMGLISTTALAAHQIALQITAILFMVPFGISMAATVRVGHAAGRQDP
HAVRRAGLMAMLLGLGLAVLFTLGIIAARVEIVQLFLGSAVADAEATVALAATLLLVGTTFFITDALQTITVGALRGIKD
TRIPLLLAVLGYWLIGFPLSYVLGLHTAFGAIGIWIGLSIGTGLYAALLVLRFLRLSR
>Mature_457_residues
TSSTATGHAPPTPPSLALELWDTIRLAAPMALTQLSQIAMMSTDLAFIGRLGGDAVAAAALAGTVYFVGSTFGMGLMSAV
APLAAQAFGADDAGLVRRALRVGLWAGLFVALPIMLIALNGETILLALCQAPGVAHLAQHYLSGLAWGVAPMLWFLALRS
FMGAVNRPEPVLWITLAAIPLNALLVYPLLYGHWGLPQLGLTGVGLATSSVNLGTFLAGLWFAAFRPPFRNYHVLAQLGR
IDWRLLRQLLVIGAPISLSFLLEYGLFSAAAILMGLISTTALAAHQIALQITAILFMVPFGISMAATVRVGHAAGRQDPH
AVRRAGLMAMLLGLGLAVLFTLGIIAARVEIVQLFLGSAVADAEATVALAATLLLVGTTFFITDALQTITVGALRGIKDT
RIPLLLAVLGYWLIGFPLSYVLGLHTAFGAIGIWIGLSIGTGLYAALLVLRFLRLSR

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI153792564, Length=441, Percent_Identity=26.0770975056689, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI22907060, Length=441, Percent_Identity=26.3038548752834, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI38261960, Length=460, Percent_Identity=24.7826086956522, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI48994890, Length=440, Percent_Identity=30.9090909090909, Blast_Score=181, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6320545, Length=405, Percent_Identity=22.4691358024691, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 48129; Mature: 47998

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSTATGHAPPTPPSLALELWDTIRLAAPMALTQLSQIAMMSTDLAFIGRLGGDAVAAA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ALAGTVYFVGSTFGMGLMSAVAPLAAQAFGADDAGLVRRALRVGLWAGLFVALPIMLIAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
NGETILLALCQAPGVAHLAQHYLSGLAWGVAPMLWFLALRSFMGAVNRPEPVLWITLAAI
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHH
PLNALLVYPLLYGHWGLPQLGLTGVGLATSSVNLGTFLAGLWFAAFRPPFRNYHVLAQLG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RIDWRLLRQLLVIGAPISLSFLLEYGLFSAAAILMGLISTTALAAHQIALQITAILFMVP
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGISMAATVRVGHAAGRQDPHAVRRAGLMAMLLGLGLAVLFTLGIIAARVEIVQLFLGSA
CCCHHHHHEEECHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VADAEATVALAATLLLVGTTFFITDALQTITVGALRGIKDTRIPLLLAVLGYWLIGFPLS
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YVLGLHTAFGAIGIWIGLSIGTGLYAALLVLRFLRLSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSSTATGHAPPTPPSLALELWDTIRLAAPMALTQLSQIAMMSTDLAFIGRLGGDAVAAA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ALAGTVYFVGSTFGMGLMSAVAPLAAQAFGADDAGLVRRALRVGLWAGLFVALPIMLIAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
NGETILLALCQAPGVAHLAQHYLSGLAWGVAPMLWFLALRSFMGAVNRPEPVLWITLAAI
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHH
PLNALLVYPLLYGHWGLPQLGLTGVGLATSSVNLGTFLAGLWFAAFRPPFRNYHVLAQLG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RIDWRLLRQLLVIGAPISLSFLLEYGLFSAAAILMGLISTTALAAHQIALQITAILFMVP
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGISMAATVRVGHAAGRQDPHAVRRAGLMAMLLGLGLAVLFTLGIIAARVEIVQLFLGSA
CCCHHHHHEEECHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VADAEATVALAATLLLVGTTFFITDALQTITVGALRGIKDTRIPLLLAVLGYWLIGFPLS
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YVLGLHTAFGAIGIWIGLSIGTGLYAALLVLRFLRLSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]