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The map label for this gene is dnaA

Identifier: 146337176

GI number: 146337176

Start: 936

End: 2366

Strand: Direct

Name: dnaA

Synonym: BRADO0001

Alternate gene names: 146337176

Gene position: 936-2366 (Clockwise)

Preceding gene: NA

Following gene: 146337177

Centisome position: 0.01

GC content: 68.13

Gene sequence:

>1431_bases
ATGACAAATTCGGAACAGGAACGCTGGTCTCGCGTGAAGGGTCGGCTGCGCTCGACCGTTGGCGAGGACGTCTATACGAG
CTGGTTTGCCCGCATGGACCTCGAAGCCGTCCAGGACGAAAGCGTCCATCTGTCGGTGCCGACGCGCTTTCTGAAGAGCT
GGATCCAGGCGCATTATGCCGAGCGCGTGCTGTCCGCCTGGCAGGCCGAAATGCCCGAGGTGCATCGCATCGACCTCTCC
GTGCGCACCGCGATGCGCTGCGCGACGCCCGCCAAGGAAGCGCCCGCCGCGGTGGAGGCGCGCCGCCCCGAGCGTAGCGA
CGCCAAGCCGGTATCCGACGCCCGCGCACCGGTCATGACTCCGGTAGCCGCCAGCCATGATGCGCTGGGCGGCTCGCCGC
TCGACCCGCGCCTGACCTTCGCGAGCTTCGTGGTCGGCCGCGCCAACACGCTCGCCCATGCGGCCGCGCGCCAGGTGGCC
GACGGCCGACGCGGCGATCCGGTGATGTTCAATCCGCTTTACATTCACGCCGGCGTCGGTCTCGGCAAGACGCATCTGCT
GCAGGCCGTGACCTGGGCCGGCAATGCCGGCGGCGAGCGCAAGGTGCTGTATCTCACCGCCGAGAAGTTCATGTATGGCT
TCGTCGCGGCGTTGAAATCGCAGACCGCGCTGGCCTTCAAGGAGGCACTGCGCGGCATCGACGTGCTCGTGATCGACGAC
CTGCAGTTCCTGCAGGGCAAGTCGACCCAGGCGGAGTTCTGTCACACCTTGAACGCGCTCATCGACGCCGGGCGCCAGGT
GGTGATCGCCGCCGACCGGCCGCCGTCCGATCTCGAGAGCCTCGACGACCGCGTCCGCTCGCGGCTCGCCGGCGGTCTCG
TCGTCGAGATGGCGACGCTCGGCGAGGACCTTCGCCTCGGCATTCTCAAGTCGCGCGTCGCGGCGGCCCGCGCGCATCAT
GCGAGCTTCGACGTGCCGGAGGCGGTGCTGGATTATCTCGCCCGCTCCATCACCCATAACGGCCGCGACCTCGAAGGCGC
GATCAACCGCCTGCTGGCGCATTCCAAGCTCAACAACCAGCCGGTCACGCTCGACATGGCCGAGCGCGAGGTACGCGATC
TGGTCCGCCCGCAGGAGCCGAAGCGGATCAAGATCGAGGACATCCAGCGCGTCGTGGCGCGGCAATACAATGTCAGCCGC
TCCGACCTCCTGTCCTCGCGCCGCACCGCCAATGTCGTGCGCCCGCGCCAGGTTGCGATGTACCTCGCCAAGACGCTGAC
CTTGCGCTCCCTGCCCGAGATCGGCCGCCGCTTCGGCGGGCGCGACCACACCACGGTGCTGCACGCCGTGCGCAAGATCG
AGGCCCTGGTCGGCAAGGACGTCGCGCTGAACGACGAGGTCGAGTCGCTCAAGCGCCAGCTGCAGGACTAG

Upstream 100 bases:

>100_bases
AGCCTCCGCTCGAGGTCTCGCTTGGCAGACCTGTTCGTATCCAGAACATCTGCACGGCGATCACACGACACACCACTCAA
TTCGAAAAGTTGCGACTGCC

Downstream 100 bases:

>100_bases
ACGCGCTCTCGCGGGGGCGCGGACAGAAGGTCCGCCCCCAGCCGCTTGTTCCCGCCACCGAACGCTCCCGCCCGGCCGGC
CCGCAGCATCGCGGAAATGC

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTNSEQERWSRVKGRLRSTVGEDVYTSWFARMDLEAVQDESVHLSVPTRFLKSWIQAHYAERVLSAWQAEMPEVHRIDLS
VRTAMRCATPAKEAPAAVEARRPERSDAKPVSDARAPVMTPVAASHDALGGSPLDPRLTFASFVVGRANTLAHAAARQVA
DGRRGDPVMFNPLYIHAGVGLGKTHLLQAVTWAGNAGGERKVLYLTAEKFMYGFVAALKSQTALAFKEALRGIDVLVIDD
LQFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVVEMATLGEDLRLGILKSRVAAARAHH
ASFDVPEAVLDYLARSITHNGRDLEGAINRLLAHSKLNNQPVTLDMAEREVRDLVRPQEPKRIKIEDIQRVVARQYNVSR
SDLLSSRRTANVVRPRQVAMYLAKTLTLRSLPEIGRRFGGRDHTTVLHAVRKIEALVGKDVALNDEVESLKRQLQD

Sequences:

>Translated_476_residues
MTNSEQERWSRVKGRLRSTVGEDVYTSWFARMDLEAVQDESVHLSVPTRFLKSWIQAHYAERVLSAWQAEMPEVHRIDLS
VRTAMRCATPAKEAPAAVEARRPERSDAKPVSDARAPVMTPVAASHDALGGSPLDPRLTFASFVVGRANTLAHAAARQVA
DGRRGDPVMFNPLYIHAGVGLGKTHLLQAVTWAGNAGGERKVLYLTAEKFMYGFVAALKSQTALAFKEALRGIDVLVIDD
LQFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVVEMATLGEDLRLGILKSRVAAARAHH
ASFDVPEAVLDYLARSITHNGRDLEGAINRLLAHSKLNNQPVTLDMAEREVRDLVRPQEPKRIKIEDIQRVVARQYNVSR
SDLLSSRRTANVVRPRQVAMYLAKTLTLRSLPEIGRRFGGRDHTTVLHAVRKIEALVGKDVALNDEVESLKRQLQD
>Mature_475_residues
TNSEQERWSRVKGRLRSTVGEDVYTSWFARMDLEAVQDESVHLSVPTRFLKSWIQAHYAERVLSAWQAEMPEVHRIDLSV
RTAMRCATPAKEAPAAVEARRPERSDAKPVSDARAPVMTPVAASHDALGGSPLDPRLTFASFVVGRANTLAHAAARQVAD
GRRGDPVMFNPLYIHAGVGLGKTHLLQAVTWAGNAGGERKVLYLTAEKFMYGFVAALKSQTALAFKEALRGIDVLVIDDL
QFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVVEMATLGEDLRLGILKSRVAAARAHHA
SFDVPEAVLDYLARSITHNGRDLEGAINRLLAHSKLNNQPVTLDMAEREVRDLVRPQEPKRIKIEDIQRVVARQYNVSRS
DLLSSRRTANVVRPRQVAMYLAKTLTLRSLPEIGRRFGGRDHTTVLHAVRKIEALVGKDVALNDEVESLKRQLQD

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=479, Percent_Identity=36.3256784968685, Blast_Score=282, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA_BRASO (A4YJ98)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001202224.1
- ProteinModelPortal:   A4YJ98
- SMR:   A4YJ98
- STRING:   A4YJ98
- GeneID:   5121399
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO0001
- eggNOG:   COG0593
- HOGENOM:   HBG695666
- OMA:   CHTLNAL
- ProtClustDB:   PRK00149
- BioCyc:   BSP376:BRADO0001-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 52691; Mature: 52560

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSEQERWSRVKGRLRSTVGEDVYTSWFARMDLEAVQDESVHLSVPTRFLKSWIQAHYA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHH
ERVLSAWQAEMPEVHRIDLSVRTAMRCATPAKEAPAAVEARRPERSDAKPVSDARAPVMT
HHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCC
PVAASHDALGGSPLDPRLTFASFVVGRANTLAHAAARQVADGRRGDPVMFNPLYIHAGVG
CCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCEEECCEEEEECCC
LGKTHLLQAVTWAGNAGGERKVLYLTAEKFMYGFVAALKSQTALAFKEALRGIDVLVIDD
CCHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
LQFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVVEMATL
HHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHC
GEDLRLGILKSRVAAARAHHASFDVPEAVLDYLARSITHNGRDLEGAINRLLAHSKLNNQ
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
PVTLDMAEREVRDLVRPQEPKRIKIEDIQRVVARQYNVSRSDLLSSRRTANVVRPRQVAM
CEEEEHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHH
YLAKTLTLRSLPEIGRRFGGRDHTTVLHAVRKIEALVGKDVALNDEVESLKRQLQD
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TNSEQERWSRVKGRLRSTVGEDVYTSWFARMDLEAVQDESVHLSVPTRFLKSWIQAHYA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHH
ERVLSAWQAEMPEVHRIDLSVRTAMRCATPAKEAPAAVEARRPERSDAKPVSDARAPVMT
HHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCC
PVAASHDALGGSPLDPRLTFASFVVGRANTLAHAAARQVADGRRGDPVMFNPLYIHAGVG
CCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCEEECCEEEEECCC
LGKTHLLQAVTWAGNAGGERKVLYLTAEKFMYGFVAALKSQTALAFKEALRGIDVLVIDD
CCHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
LQFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVVEMATL
HHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHC
GEDLRLGILKSRVAAARAHHASFDVPEAVLDYLARSITHNGRDLEGAINRLLAHSKLNNQ
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
PVTLDMAEREVRDLVRPQEPKRIKIEDIQRVVARQYNVSRSDLLSSRRTANVVRPRQVAM
CEEEEHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHH
YLAKTLTLRSLPEIGRRFGGRDHTTVLHAVRKIEALVGKDVALNDEVESLKRQLQD
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA