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The map label for this gene is hemK [H]

Identifier: 146302781

GI number: 146302781

Start: 6094651

End: 6095505

Strand: Reverse

Name: hemK [H]

Synonym: Fjoh_5054

Alternate gene names: 146302781

Gene position: 6095505-6094651 (Counterclockwise)

Preceding gene: 146302782

Following gene: 146302779

Centisome position: 99.98

GC content: 30.99

Gene sequence:

>855_bases
ATGAAAATCAAGCAATATCGTACTCAGTTTATTAAGGAATTATCTTCTTTGTACGATGCGTACGAAGCAGAGAGTTTTTT
CTATTTAATTTTAGAAGATAAACACAAACTGCGTCAAATAGATTTAGCATTAAATCACGAATTAGCTTTTACAGAAGAAG
ATTTTGTTGTTTGGGATTCATTGTTAGCCCAGCTGAAAAAAGAAGTTCCAATTCAATATTTATTAGGAAAAACAAATTTC
TACGGTTTGGATTTTGAAGTAAACGAAAATGTTTTAATTCCGAGACCCGAAACCGAAGAGTTAGTCGAATGGATTATTAG
TGAAAATAAAGGAATTGATAGAAAAGAAAAAATAAAAATTCTTGATATTGGTACCGGAAGCGGCTGTATTGCTGTCTCAC
TCGCTAAAAATCTGCCAAATGCAGATGTTTATGCTATCGATGTTTCAAAAAAAGCAATCGAAACTGCGAAGCGAAATGCC
ATTAGAAATGATGTTAATGTAACTTTTATATTTCAGGATATTTTACAAGCCGAAGAATTAAAATGTAAATATGATGTCAT
AGTTTCTAATCCGCCTTATATACGCAATCTTGAAAAAGAAGAAATCAAAAAGAACGTTTTGGATTATGAACCTCATTTAG
CACTTTTTGTTGATGACAACGATGCTTTACTTTTCTACAGAAAAATAGCTTCGCTGGCACAAAACAATCTTTTGGCAAAG
GGACAGCTTTATTTTGAAATCAATCAATATTTAGGAAAAGAAATGATAGAACTTCTGGGTAAAATGGATTTTATCAATAT
TGATCTCCGAAAAGATATTTACGATAACGACCGAATGATAAAAGTTAATATTTAA

Upstream 100 bases:

>100_bases
CAAAAGCTGAAAATGAAAATTCAGTAAAAAAAGTTTCAGAAATAGCAGTACAATTTGACTGCTAACTAACTAAAATATTA
TCTAATAACAGATATATAAA

Downstream 100 bases:

>100_bases
ATTTTAGTTTTTAGTCGCAGTATTCAGTCACAGTATTCAGTCACGGTTAAGCTGGGAACTGCGACTGCGACTGAAAACTA
AAAAAAATTACTCGTATCGC

Product: HemK family modification methylase

Products: NA

Alternate protein names: HemK protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKIKQYRTQFIKELSSLYDAYEAESFFYLILEDKHKLRQIDLALNHELAFTEEDFVVWDSLLAQLKKEVPIQYLLGKTNF
YGLDFEVNENVLIPRPETEELVEWIISENKGIDRKEKIKILDIGTGSGCIAVSLAKNLPNADVYAIDVSKKAIETAKRNA
IRNDVNVTFIFQDILQAEELKCKYDVIVSNPPYIRNLEKEEIKKNVLDYEPHLALFVDDNDALLFYRKIASLAQNNLLAK
GQLYFEINQYLGKEMIELLGKMDFINIDLRKDIYDNDRMIKVNI

Sequences:

>Translated_284_residues
MKIKQYRTQFIKELSSLYDAYEAESFFYLILEDKHKLRQIDLALNHELAFTEEDFVVWDSLLAQLKKEVPIQYLLGKTNF
YGLDFEVNENVLIPRPETEELVEWIISENKGIDRKEKIKILDIGTGSGCIAVSLAKNLPNADVYAIDVSKKAIETAKRNA
IRNDVNVTFIFQDILQAEELKCKYDVIVSNPPYIRNLEKEEIKKNVLDYEPHLALFVDDNDALLFYRKIASLAQNNLLAK
GQLYFEINQYLGKEMIELLGKMDFINIDLRKDIYDNDRMIKVNI
>Mature_284_residues
MKIKQYRTQFIKELSSLYDAYEAESFFYLILEDKHKLRQIDLALNHELAFTEEDFVVWDSLLAQLKKEVPIQYLLGKTNF
YGLDFEVNENVLIPRPETEELVEWIISENKGIDRKEKIKILDIGTGSGCIAVSLAKNLPNADVYAIDVSKKAIETAKRNA
IRNDVNVTFIFQDILQAEELKCKYDVIVSNPPYIRNLEKEEIKKNVLDYEPHLALFVDDNDALLFYRKIASLAQNNLLAK
GQLYFEINQYLGKEMIELLGKMDFINIDLRKDIYDNDRMIKVNI

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=231, Percent_Identity=27.7056277056277, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1787463, Length=233, Percent_Identity=29.1845493562232, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87082085, Length=211, Percent_Identity=32.2274881516588, Blast_Score=102, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6324266, Length=200, Percent_Identity=36.5, Blast_Score=99, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24582226, Length=187, Percent_Identity=32.0855614973262, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR022438
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 33086; Mature: 33086

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKQYRTQFIKELSSLYDAYEAESFFYLILEDKHKLRQIDLALNHELAFTEEDFVVWDS
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHEEEHHEECCEEEECCCCHHHHHH
LLAQLKKEVPIQYLLGKTNFYGLDFEVNENVLIPRPETEELVEWIISENKGIDRKEKIKI
HHHHHHHHCCEEEEECCCCEEEEEEEECCCEEECCCCHHHHHHHHHHCCCCCCHHHCEEE
LDIGTGSGCIAVSLAKNLPNADVYAIDVSKKAIETAKRNAIRNDVNVTFIFQDILQAEEL
EEECCCCCEEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHC
KCKYDVIVSNPPYIRNLEKEEIKKNVLDYEPHLALFVDDNDALLFYRKIASLAQNNLLAK
CEEEEEEECCCCHHHCCCHHHHHHHHCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCEEC
GQLYFEINQYLGKEMIELLGKMDFINIDLRKDIYDNDRMIKVNI
CEEEEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEC
>Mature Secondary Structure
MKIKQYRTQFIKELSSLYDAYEAESFFYLILEDKHKLRQIDLALNHELAFTEEDFVVWDS
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHEEEHHEECCEEEECCCCHHHHHH
LLAQLKKEVPIQYLLGKTNFYGLDFEVNENVLIPRPETEELVEWIISENKGIDRKEKIKI
HHHHHHHHCCEEEEECCCCEEEEEEEECCCEEECCCCHHHHHHHHHHCCCCCCHHHCEEE
LDIGTGSGCIAVSLAKNLPNADVYAIDVSKKAIETAKRNAIRNDVNVTFIFQDILQAEEL
EEECCCCCEEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHC
KCKYDVIVSNPPYIRNLEKEEIKKNVLDYEPHLALFVDDNDALLFYRKIASLAQNNLLAK
CEEEEEEECCCCHHHCCCHHHHHHHHCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCEEC
GQLYFEINQYLGKEMIELLGKMDFINIDLRKDIYDNDRMIKVNI
CEEEEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA