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The map label for this gene is hisS

Identifier: 146302779

GI number: 146302779

Start: 6091653

End: 6093080

Strand: Reverse

Name: hisS

Synonym: Fjoh_5052

Alternate gene names: 146302779

Gene position: 6093080-6091653 (Counterclockwise)

Preceding gene: 146302781

Following gene: 146302778

Centisome position: 99.94

GC content: 33.82

Gene sequence:

>1428_bases
ATGGCTTCAAAACCAAGTATACCAAAAGGAACAAGAGATTTTTCACCAGCAGAGGTGTCAAAACGTCAATATATTATTCA
GACTATAAAAGCTAATTTTGAAAAATTTGGTTTTCAGCCAATCGAAACGCCTTCGTTTGAAAATTCAGATACCTTAATGG
GGAAATATGGAGAAGAAGGAGATCGTCTGATTTTTAAAATTTTAAATTCAGGTAATTTTTTCTACAATAAAAGTAAAATC
GAATTGCCAGAATCGATCGAAGAATTACAGCTAAATTCTGCTGAAAAAATAACTTTAGAGCAAAGAATTGAGCTGAATAA
ATTCACAGGGAAAATTTCTGAAAAAGCGTTGCGTTACGATTTAACAGTGCCGTTTGCAAGATACGTTGTACAACACCAAA
GTGAAATCGAATTTCCTTTTAAAAGATACCAAATCCAACCGGTTTGGAGAGCAGATAATCCGCAAAAAGGTCGTTTTAGA
GAATTTTTTCAATGTGATGCTGATGTTGTAGGTTCAAAATCTTTATGGCAGGAAGTAGAATTGGTTCAATTGTACGATAC
TGTTTTTACTTCTTTAGGTTTAGAAGGAGTTACTATTAAAATCAATAACCGAAAAATTTTATCTGGAATTGCTGAGGTAA
TTGGCGCTTCAGATAAATTAATCGACTTTACAGTTGCCCTTGATAAATTAGACAAAATTGGTGAAGACGGCGTAAAGAAA
GAAATGATCGAAAAAGGAATTTCTGAAGATGCTTTAGTAAAAGTGCAGCCACTTTTTAATTTCTCAGGAACTTTTGCAGA
CAAAATTGCACAGCTTTCGGACTTATTGTCTTCTTCTGAAGAAGGAATGAAAGGAGTTGAAGAATTGAAGTTTATTTGTG
ACAATGTTGCAGATTTAGGTTTGTCTACTGCAGTTTTAGATCTTGATGTGACATTAGCTCGTGGTTTAAATTATTATACC
GGAGCTATTTTTGAAGTTGCAGCGCCAAAAACCGTTGCAATGGGTTCTATTGGCGGAGGCGGAAGATACGACGATTTGAC
GGGTATTTTTGGTTTGAAAAATATGAGCGGTGTTGGAATCTCTTTTGGATTGGATAGAATTTATCTGGTTTTAGAAGAAT
TGCAATTATTTCCTGAAACTGTAGCTGCTACTTCAAAAGCAATATTCTTGAATTTTGGAGACAAAGAAGCTTTGTATGCT
TCAAAAGCCATTCAGAAATTAAGACAGGAAAATATAAAAGTAGAGTTATATCCGGATAATGTAAAAGTAGGTAAACAATT
TCAATATGCAGATAAGCGTTTGATTCCGTTTGCTGTATTAGTTGGAGATCAGGAAATTAATTCAAATTCTTATGCGCTTA
AAAATTTAGTGACAGGAGAACAGGTGTCTGTAGATTTTGAAGGATTGAAAAAAACTTTGTTAGGTTAA

Upstream 100 bases:

>100_bases
CAGCAATCAAAAAATAATAAATAAATTAATCTTAAAGGTTTTTGAATTTGAGTTTCAAAAGAAATCTAAAATCTAAAATC
AACAATCTAAAATAAAGAAG

Downstream 100 bases:

>100_bases
CTGTCTGTAGCGAAGGTTTTTGCTTTAGATTGCGGTCATTTAAAGGATTTTCTTTTTAAATCTCTGCCTAAAATAGTTGC
CTGTAAAGAAAAAAAACTTT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MASKPSIPKGTRDFSPAEVSKRQYIIQTIKANFEKFGFQPIETPSFENSDTLMGKYGEEGDRLIFKILNSGNFFYNKSKI
ELPESIEELQLNSAEKITLEQRIELNKFTGKISEKALRYDLTVPFARYVVQHQSEIEFPFKRYQIQPVWRADNPQKGRFR
EFFQCDADVVGSKSLWQEVELVQLYDTVFTSLGLEGVTIKINNRKILSGIAEVIGASDKLIDFTVALDKLDKIGEDGVKK
EMIEKGISEDALVKVQPLFNFSGTFADKIAQLSDLLSSSEEGMKGVEELKFICDNVADLGLSTAVLDLDVTLARGLNYYT
GAIFEVAAPKTVAMGSIGGGGRYDDLTGIFGLKNMSGVGISFGLDRIYLVLEELQLFPETVAATSKAIFLNFGDKEALYA
SKAIQKLRQENIKVELYPDNVKVGKQFQYADKRLIPFAVLVGDQEINSNSYALKNLVTGEQVSVDFEGLKKTLLG

Sequences:

>Translated_475_residues
MASKPSIPKGTRDFSPAEVSKRQYIIQTIKANFEKFGFQPIETPSFENSDTLMGKYGEEGDRLIFKILNSGNFFYNKSKI
ELPESIEELQLNSAEKITLEQRIELNKFTGKISEKALRYDLTVPFARYVVQHQSEIEFPFKRYQIQPVWRADNPQKGRFR
EFFQCDADVVGSKSLWQEVELVQLYDTVFTSLGLEGVTIKINNRKILSGIAEVIGASDKLIDFTVALDKLDKIGEDGVKK
EMIEKGISEDALVKVQPLFNFSGTFADKIAQLSDLLSSSEEGMKGVEELKFICDNVADLGLSTAVLDLDVTLARGLNYYT
GAIFEVAAPKTVAMGSIGGGGRYDDLTGIFGLKNMSGVGISFGLDRIYLVLEELQLFPETVAATSKAIFLNFGDKEALYA
SKAIQKLRQENIKVELYPDNVKVGKQFQYADKRLIPFAVLVGDQEINSNSYALKNLVTGEQVSVDFEGLKKTLLG
>Mature_474_residues
ASKPSIPKGTRDFSPAEVSKRQYIIQTIKANFEKFGFQPIETPSFENSDTLMGKYGEEGDRLIFKILNSGNFFYNKSKIE
LPESIEELQLNSAEKITLEQRIELNKFTGKISEKALRYDLTVPFARYVVQHQSEIEFPFKRYQIQPVWRADNPQKGRFRE
FFQCDADVVGSKSLWQEVELVQLYDTVFTSLGLEGVTIKINNRKILSGIAEVIGASDKLIDFTVALDKLDKIGEDGVKKE
MIEKGISEDALVKVQPLFNFSGTFADKIAQLSDLLSSSEEGMKGVEELKFICDNVADLGLSTAVLDLDVTLARGLNYYTG
AIFEVAAPKTVAMGSIGGGGRYDDLTGIFGLKNMSGVGISFGLDRIYLVLEELQLFPETVAATSKAIFLNFGDKEALYAS
KAIQKLRQENIKVELYPDNVKVGKQFQYADKRLIPFAVLVGDQEINSNSYALKNLVTGEQVSVDFEGLKKTLLG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=482, Percent_Identity=31.3278008298755, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI15029520, Length=480, Percent_Identity=29.375, Blast_Score=208, Evalue=9e-54,
Organism=Escherichia coli, GI1788861, Length=484, Percent_Identity=25, Blast_Score=109, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71993693, Length=495, Percent_Identity=33.5353535353535, Blast_Score=241, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI71993686, Length=495, Percent_Identity=33.5353535353535, Blast_Score=241, Evalue=7e-64,
Organism=Saccharomyces cerevisiae, GI6325290, Length=497, Percent_Identity=32.5955734406439, Blast_Score=198, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24643061, Length=504, Percent_Identity=31.547619047619, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24643059, Length=504, Percent_Identity=31.547619047619, Blast_Score=206, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_FLAJ1 (A5F9R5)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001197370.1
- ProteinModelPortal:   A5F9R5
- SMR:   A5F9R5
- STRING:   A5F9R5
- GeneID:   5094137
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_5052
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   YVAEHEH
- ProtClustDB:   PRK00037
- BioCyc:   FJOH376686:FJOH_5052-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 52941; Mature: 52810

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKPSIPKGTRDFSPAEVSKRQYIIQTIKANFEKFGFQPIETPSFENSDTLMGKYGEEG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCC
DRLIFKILNSGNFFYNKSKIELPESIEELQLNSAEKITLEQRIELNKFTGKISEKALRYD
CCEEEEEECCCCEEEECCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHEEE
LTVPFARYVVQHQSEIEFPFKRYQIQPVWRADNPQKGRFREFFQCDADVVGSKSLWQEVE
CCCHHHHHHHHHCCCCCCCHHHEEEEEEECCCCCCCCHHHHHHHCCCHHCCCHHHHHHHH
LVQLYDTVFTSLGLEGVTIKINNRKILSGIAEVIGASDKLIDFTVALDKLDKIGEDGVKK
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCHHH
EMIEKGISEDALVKVQPLFNFSGTFADKIAQLSDLLSSSEEGMKGVEELKFICDNVADLG
HHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
LSTAVLDLDVTLARGLNYYTGAIFEVAAPKTVAMGSIGGGGRYDDLTGIFGLKNMSGVGI
CCEEEEEEHHHHHHCCCHHHCCEEEECCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCH
SFGLDRIYLVLEELQLFPETVAATSKAIFLNFGDKEALYASKAIQKLRQENIKVELYPDN
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCC
VKVGKQFQYADKRLIPFAVLVGDQEINSNSYALKNLVTGEQVSVDFEGLKKTLLG
CCCCCCHHHCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHCC
>Mature Secondary Structure 
ASKPSIPKGTRDFSPAEVSKRQYIIQTIKANFEKFGFQPIETPSFENSDTLMGKYGEEG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCC
DRLIFKILNSGNFFYNKSKIELPESIEELQLNSAEKITLEQRIELNKFTGKISEKALRYD
CCEEEEEECCCCEEEECCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHEEE
LTVPFARYVVQHQSEIEFPFKRYQIQPVWRADNPQKGRFREFFQCDADVVGSKSLWQEVE
CCCHHHHHHHHHCCCCCCCHHHEEEEEEECCCCCCCCHHHHHHHCCCHHCCCHHHHHHHH
LVQLYDTVFTSLGLEGVTIKINNRKILSGIAEVIGASDKLIDFTVALDKLDKIGEDGVKK
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCHHH
EMIEKGISEDALVKVQPLFNFSGTFADKIAQLSDLLSSSEEGMKGVEELKFICDNVADLG
HHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
LSTAVLDLDVTLARGLNYYTGAIFEVAAPKTVAMGSIGGGGRYDDLTGIFGLKNMSGVGI
CCEEEEEEHHHHHHCCCHHHCCEEEECCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCH
SFGLDRIYLVLEELQLFPETVAATSKAIFLNFGDKEALYASKAIQKLRQENIKVELYPDN
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCC
VKVGKQFQYADKRLIPFAVLVGDQEINSNSYALKNLVTGEQVSVDFEGLKKTLLG
CCCCCCHHHCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA