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The map label for this gene is grpE [H]

Identifier: 146302778

GI number: 146302778

Start: 6090571

End: 6091149

Strand: Reverse

Name: grpE [H]

Synonym: Fjoh_5051

Alternate gene names: 146302778

Gene position: 6091149-6090571 (Counterclockwise)

Preceding gene: 146302779

Following gene: 146302777

Centisome position: 99.91

GC content: 32.3

Gene sequence:

>579_bases
ATGAAGTTTAAGAATATTTTTAAAAATAAAAGTAATATGACTACGGAAAATACAGAATTCGATCAGGAATTAGACGATGT
AACGTTAGAGAACAATGCAAACGGAGAGCAGTTAATTGTTGAAGAATTAAGTGTTGAGGAGCAATTAGCCCAAGACTTGG
CGAAAGAAAAAGATAAGTTTCTGAGATTATTTGCTGAATTTGAAAATTACAAAAAAAGAACTTCAAAAGAGCGTATCGAT
TTATTTAAAACGGCAAACCAAGAAGTTTTATTGGCTATGCTTCCTGTTTTAGATGATTTCGACAGAGCAGCTGTAGAGAT
CAACAAATCTGATGATGAAAATTTGAAAAAAGGTGTTGAACTGATTCACGAAAAATTAAAAAGCACTTTAGTTTCTAAAG
GCTTAGAGCAGGTTGAAATTCAGGCAGGAGATGCATTCAATGCTGATATTGCTGAAGCAATTACCCAAATTCCAGCTCCA
TCGGATAAATTAAAAGGGAAAGTTGTAGATGTTATTGAAAAAGGATACAAATTAGGAGAAAAAATTATTCGTTATCCTAA
AGTTGTTGTTGGAAACTAA

Upstream 100 bases:

>100_bases
CTGTTTTCTGTTTTTAAAAGTCTCATAACTAATAAAGTGACAATTTGACATTTTATGAGATTTGGCAGTACTTTTGCAAT
CCAAAAGAAAGAAATAAAAA

Downstream 100 bases:

>100_bases
AAAATGCAATAAATTTCAAAAGCATTTTTTGGATTTTGGAATTTTAAGATTTGGAACTTAATCTAATTATGAAAAAAGAT
TTTTACGAAATACTAGGCAT

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MKFKNIFKNKSNMTTENTEFDQELDDVTLENNANGEQLIVEELSVEEQLAQDLAKEKDKFLRLFAEFENYKKRTSKERID
LFKTANQEVLLAMLPVLDDFDRAAVEINKSDDENLKKGVELIHEKLKSTLVSKGLEQVEIQAGDAFNADIAEAITQIPAP
SDKLKGKVVDVIEKGYKLGEKIIRYPKVVVGN

Sequences:

>Translated_192_residues
MKFKNIFKNKSNMTTENTEFDQELDDVTLENNANGEQLIVEELSVEEQLAQDLAKEKDKFLRLFAEFENYKKRTSKERID
LFKTANQEVLLAMLPVLDDFDRAAVEINKSDDENLKKGVELIHEKLKSTLVSKGLEQVEIQAGDAFNADIAEAITQIPAP
SDKLKGKVVDVIEKGYKLGEKIIRYPKVVVGN
>Mature_192_residues
MKFKNIFKNKSNMTTENTEFDQELDDVTLENNANGEQLIVEELSVEEQLAQDLAKEKDKFLRLFAEFENYKKRTSKERID
LFKTANQEVLLAMLPVLDDFDRAAVEINKSDDENLKKGVELIHEKLKSTLVSKGLEQVEIQAGDAFNADIAEAITQIPAP
SDKLKGKVVDVIEKGYKLGEKIIRYPKVVVGN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=189, Percent_Identity=29.1005291005291, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21791; Mature: 21791

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKNIFKNKSNMTTENTEFDQELDDVTLENNANGEQLIVEELSVEEQLAQDLAKEKDKF
CCCHHHHCCCCCCCCCCCHHHHHHHCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRLFAEFENYKKRTSKERIDLFKTANQEVLLAMLPVLDDFDRAAVEINKSDDENLKKGVE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHEEECCCCHHHHHHHHH
LIHEKLKSTLVSKGLEQVEIQAGDAFNADIAEAITQIPAPSDKLKGKVVDVIEKGYKLGE
HHHHHHHHHHHHCCHHHEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KIIRYPKVVVGN
HHHHCCCHHCCC
>Mature Secondary Structure
MKFKNIFKNKSNMTTENTEFDQELDDVTLENNANGEQLIVEELSVEEQLAQDLAKEKDKF
CCCHHHHCCCCCCCCCCCHHHHHHHCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRLFAEFENYKKRTSKERIDLFKTANQEVLLAMLPVLDDFDRAAVEINKSDDENLKKGVE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHEEECCCCHHHHHHHHH
LIHEKLKSTLVSKGLEQVEIQAGDAFNADIAEAITQIPAPSDKLKGKVVDVIEKGYKLGE
HHHHHHHHHHHHCCHHHEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KIIRYPKVVVGN
HHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA