Click here to switch to the map view.

The map label for this gene is trmH [C]

Identifier: 146302736

GI number: 146302736

Start: 6047475

End: 6048137

Strand: Reverse

Name: trmH [C]

Synonym: Fjoh_5009

Alternate gene names: 146302736

Gene position: 6048137-6047475 (Counterclockwise)

Preceding gene: 146302737

Following gene: 146302727

Centisome position: 99.2

GC content: 34.84

Gene sequence:

>663_bases
ATGATTGATTTAGATTACCTGGCATTTCTTGAAGATATATTAACAGATAACCGAAAAGAGAAATTTCTAAAAGTATTAGA
AAATCGTACGAAGCATTTTACCATTGCTGTCGAAGATGTTTACCAAATGCATAACACGAGTGCTGTAATGCGCAGTTGTG
AAGTTTTTGGAATTCAGGAACTTAATATTATCGAACAGCGTTTTGGAAAAAGGATCGATAAAGAAATCGCTTTGGGTGCT
CAAAAATGGGTTGACATAAACCGATTTGATACTGTCGACGGCTGTATTTCGTCTTTAAGAGATAAAGGATATCAAATTAT
TGCGACAACGCCGCACGAAAATGACTGCTTGATTCAGGATTTTGATATTTCAAAACCAAGTGCCTTATTTTTTGGAACTG
AAAAAGATGGTCTTTCGCCGGAAATTCTTGAAAAAGCAGATGGTTTTCTTAAAATTCCAATGGTAGGGTTTACAGAGAGT
TTGAACATTTCTGTTTCGGCTGCAATTATCATCCAAAACTTAACCAACAGATTACGAAATTCAGATATCAAATGGAAACT
GTCTGATGAAGAAATACTGCAAAAACGTCTTGACTGGGCTAAAAATTCAATTAGAGATATTAAGAGAATCGAAGCACGTT
ATTATCAGGATAACCCAAGATAA

Upstream 100 bases:

>100_bases
ATTTAAATAATATACTTTTGGAGTACTCAGAACGTTGAAAATGCAAATCAATTTTCATTTTGTTTATTTCTAATGTATTT
TTGCGCCAAATACATACAAA

Downstream 100 bases:

>100_bases
ATTGCAAGATAAAAATTAGCCGTAAATTCGAATTTATGAATTCGAATTTACGGCTAAAAAAACTTAGAACCGTAGTTCTT
CAGTACCTTAGCACCTAAAA

Product: tRNA/rRNA methyltransferase SpoU

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MIDLDYLAFLEDILTDNRKEKFLKVLENRTKHFTIAVEDVYQMHNTSAVMRSCEVFGIQELNIIEQRFGKRIDKEIALGA
QKWVDINRFDTVDGCISSLRDKGYQIIATTPHENDCLIQDFDISKPSALFFGTEKDGLSPEILEKADGFLKIPMVGFTES
LNISVSAAIIIQNLTNRLRNSDIKWKLSDEEILQKRLDWAKNSIRDIKRIEARYYQDNPR

Sequences:

>Translated_220_residues
MIDLDYLAFLEDILTDNRKEKFLKVLENRTKHFTIAVEDVYQMHNTSAVMRSCEVFGIQELNIIEQRFGKRIDKEIALGA
QKWVDINRFDTVDGCISSLRDKGYQIIATTPHENDCLIQDFDISKPSALFFGTEKDGLSPEILEKADGFLKIPMVGFTES
LNISVSAAIIIQNLTNRLRNSDIKWKLSDEEILQKRLDWAKNSIRDIKRIEARYYQDNPR
>Mature_220_residues
MIDLDYLAFLEDILTDNRKEKFLKVLENRTKHFTIAVEDVYQMHNTSAVMRSCEVFGIQELNIIEQRFGKRIDKEIALGA
QKWVDINRFDTVDGCISSLRDKGYQIIATTPHENDCLIQDFDISKPSALFFGTEKDGLSPEILEKADGFLKIPMVGFTES
LNISVSAAIIIQNLTNRLRNSDIKWKLSDEEILQKRLDWAKNSIRDIKRIEARYYQDNPR

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=170, Percent_Identity=28.2352941176471, Blast_Score=104, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI193204953, Length=139, Percent_Identity=25.8992805755396, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 25492; Mature: 25492

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLDYLAFLEDILTDNRKEKFLKVLENRTKHFTIAVEDVYQMHNTSAVMRSCEVFGIQE
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHH
LNIIEQRFGKRIDKEIALGAQKWVDINRFDTVDGCISSLRDKGYQIIATTPHENDCLIQD
HHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCEEEE
FDISKPSALFFGTEKDGLSPEILEKADGFLKIPMVGFTESLNISVSAAIIIQNLTNRLRN
CCCCCCCEEEEECCCCCCCHHHHHHCCCEEEECEECCCCCCCEEHHHHHHHHHHHHHHCC
SDIKWKLSDEEILQKRLDWAKNSIRDIKRIEARYYQDNPR
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIDLDYLAFLEDILTDNRKEKFLKVLENRTKHFTIAVEDVYQMHNTSAVMRSCEVFGIQE
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHH
LNIIEQRFGKRIDKEIALGAQKWVDINRFDTVDGCISSLRDKGYQIIATTPHENDCLIQD
HHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCEEEE
FDISKPSALFFGTEKDGLSPEILEKADGFLKIPMVGFTESLNISVSAAIIIQNLTNRLRN
CCCCCCCEEEEECCCCCCCHHHHHHCCCEEEECEECCCCCCCEEHHHHHHHHHHHHHHCC
SDIKWKLSDEEILQKRLDWAKNSIRDIKRIEARYYQDNPR
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]