Click here to switch to the map view.

The map label for this gene is menE [H]

Identifier: 146302509

GI number: 146302509

Start: 5777423

End: 5778469

Strand: Reverse

Name: menE [H]

Synonym: Fjoh_4782

Alternate gene names: 146302509

Gene position: 5778469-5777423 (Counterclockwise)

Preceding gene: 146302510

Following gene: 146302505

Centisome position: 94.78

GC content: 34.38

Gene sequence:

>1047_bases
ATGCTAAACTTAACTCATAAAAACGTACATAACTATTTTAAGCTCGACGGTTATCATTTAAATGCAGCTGATTTATGCCG
AATTGGCTACAGTTTTATTAAAGAAGGCGATGCCTATGAGCGCGCAATTGGTGAATTTTTTCTGGATTGGTTCGATCATA
AGGACTATATCGAAATGACAACATCAGGAACAACCGGACTTCCTAAATTAGTAAGATTAGAAAAACAAGCCATGATTCAG
TCTGCATTGGCAACAGGAGATTTCTTCGGATTAGAGCCGGGAAATAAAGCATTATTGTGTCTGCCGACTCAGTTTATTGC
AGGAAAAATGATGCTTGTGCGCAGTTTGATTTTAGGTCTGGAATTAGATGTTACACTACCAAGTACTGAGCCTTTGGCTT
ACAATAATAAGCAATATGATTTTGTAGCAATGGTTCCGTTACAAGTTCAAAATTCAATTGAAAAGCTGGGAAATATTAAA
AAGCTGATTATTGGCGGTGCAAAAATGGATAGTGCTCTCGAAGAAAAGCTTTTGCCATTAAAGAAAACAGAAATTTACGA
AACCTACGGAATGACAGAAACAATTACGCATATTGCGGCTAAAAAAGTAGGTGAAACTGCTTTTACGATTCTGCCAAATG
TTAAAATTGAGAAAGATGACCGAGGCTGTTTAGTTATTTATGTTGCTTCGATTTCTGATGAACCAATTGTAACCAATGAT
TTAGTTGATTTAGTAAATGAAAATCAGTTTGTTTTTTTGGGAAGAATCGATAATGTCATTAACAGCGGGGGAGTAAAGCT
GATTCCTGAACAGATTGAAGCTAAATTAACAGGCAGAATTACAAACCGATTTTTTGTAACTGGTGTTCCTGATACAGTTT
TAGGAGAAAAATTGATTTTAGTAATTGAAGGGGAGAAGTATGATTTTTCGGCTGATTTTTTTGATGTTTTAGGAAAATTT
GAAAAACCCAAAGAAATTGTTTTTGTTCCGAAATTTAAAGAAAATGAAAACGGAAAGTTATTACGCAAATCTAGTTTGAA
AGTTTAA

Upstream 100 bases:

>100_bases
AAAAATACAAATGGACAAACTGGAAAGAAAAACTAACCGGAATAATTGACGAAAAAAATAATGAAACAGCAGAATTTTTA
AACTTAAATTAAAATTTATT

Downstream 100 bases:

>100_bases
AACTTTAATTTGAGGAGTATTCTTTTTTATTAATATGTAATCTCTAAGAGATATTTGAGAACTCCTTTTGGAGTTGTAGA
TCTAGACAAGAACCACATAA

Product: Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II-like protein

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MLNLTHKNVHNYFKLDGYHLNAADLCRIGYSFIKEGDAYERAIGEFFLDWFDHKDYIEMTTSGTTGLPKLVRLEKQAMIQ
SALATGDFFGLEPGNKALLCLPTQFIAGKMMLVRSLILGLELDVTLPSTEPLAYNNKQYDFVAMVPLQVQNSIEKLGNIK
KLIIGGAKMDSALEEKLLPLKKTEIYETYGMTETITHIAAKKVGETAFTILPNVKIEKDDRGCLVIYVASISDEPIVTND
LVDLVNENQFVFLGRIDNVINSGGVKLIPEQIEAKLTGRITNRFFVTGVPDTVLGEKLILVIEGEKYDFSADFFDVLGKF
EKPKEIVFVPKFKENENGKLLRKSSLKV

Sequences:

>Translated_348_residues
MLNLTHKNVHNYFKLDGYHLNAADLCRIGYSFIKEGDAYERAIGEFFLDWFDHKDYIEMTTSGTTGLPKLVRLEKQAMIQ
SALATGDFFGLEPGNKALLCLPTQFIAGKMMLVRSLILGLELDVTLPSTEPLAYNNKQYDFVAMVPLQVQNSIEKLGNIK
KLIIGGAKMDSALEEKLLPLKKTEIYETYGMTETITHIAAKKVGETAFTILPNVKIEKDDRGCLVIYVASISDEPIVTND
LVDLVNENQFVFLGRIDNVINSGGVKLIPEQIEAKLTGRITNRFFVTGVPDTVLGEKLILVIEGEKYDFSADFFDVLGKF
EKPKEIVFVPKFKENENGKLLRKSSLKV
>Mature_348_residues
MLNLTHKNVHNYFKLDGYHLNAADLCRIGYSFIKEGDAYERAIGEFFLDWFDHKDYIEMTTSGTTGLPKLVRLEKQAMIQ
SALATGDFFGLEPGNKALLCLPTQFIAGKMMLVRSLILGLELDVTLPSTEPLAYNNKQYDFVAMVPLQVQNSIEKLGNIK
KLIIGGAKMDSALEEKLLPLKKTEIYETYGMTETITHIAAKKVGETAFTILPNVKIEKDDRGCLVIYVASISDEPIVTND
LVDLVNENQFVFLGRIDNVINSGGVKLIPEQIEAKLTGRITNRFFVTGVPDTVLGEKLILVIEGEKYDFSADFFDVLGKF
EKPKEIVFVPKFKENENGKLLRKSSLKV

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=307, Percent_Identity=25.0814332247557, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 39000; Mature: 39000

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLTHKNVHNYFKLDGYHLNAADLCRIGYSFIKEGDAYERAIGEFFLDWFDHKDYIEMT
CCCCCCCCCCCEEEECCEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEE
TSGTTGLPKLVRLEKQAMIQSALATGDFFGLEPGNKALLCLPTQFIAGKMMLVRSLILGL
CCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEECHHHHHHHHHHHHHHHHCC
ELDVTLPSTEPLAYNNKQYDFVAMVPLQVQNSIEKLGNIKKLIIGGAKMDSALEEKLLPL
EEEEECCCCCCCEECCCCEEEEEEECHHHHHHHHHHCCEEEEEEECCHHHHHHHHHCCCC
KKTEIYETYGMTETITHIAAKKVGETAFTILPNVKIEKDDRGCLVIYVASISDEPIVTND
CHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCEEECCCCCEEEEEEEECCCCCCCHHH
LVDLVNENQFVFLGRIDNVINSGGVKLIPEQIEAKLTGRITNRFFVTGVPDTVLGEKLIL
HHHHHCCCCEEEEEEHHHHHCCCCCEEEHHHHHHHHHEEEECEEEEECCCCHHCCCEEEE
VIEGEKYDFSADFFDVLGKFEKPKEIVFVPKFKENENGKLLRKSSLKV
EEECCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCEEECCCCCC
>Mature Secondary Structure
MLNLTHKNVHNYFKLDGYHLNAADLCRIGYSFIKEGDAYERAIGEFFLDWFDHKDYIEMT
CCCCCCCCCCCEEEECCEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEE
TSGTTGLPKLVRLEKQAMIQSALATGDFFGLEPGNKALLCLPTQFIAGKMMLVRSLILGL
CCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEECHHHHHHHHHHHHHHHHCC
ELDVTLPSTEPLAYNNKQYDFVAMVPLQVQNSIEKLGNIKKLIIGGAKMDSALEEKLLPL
EEEEECCCCCCCEECCCCEEEEEEECHHHHHHHHHHCCEEEEEEECCHHHHHHHHHCCCC
KKTEIYETYGMTETITHIAAKKVGETAFTILPNVKIEKDDRGCLVIYVASISDEPIVTND
CHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCEEECCCCCEEEEEEEECCCCCCCHHH
LVDLVNENQFVFLGRIDNVINSGGVKLIPEQIEAKLTGRITNRFFVTGVPDTVLGEKLIL
HHHHHCCCCEEEEEEHHHHHCCCCCEEEHHHHHHHHHEEEECEEEEECCCCHHCCCEEEE
VIEGEKYDFSADFFDVLGKFEKPKEIVFVPKFKENENGKLLRKSSLKV
EEECCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]